>KMC005661A_C01 KMC005661A_c01
aaatcaaatcccttagatttattaataaaaattaacatccaggatattcatggctcaAAT
CCAAGACCTCTATTTAGAGGGATCAAGCCAGATGTAGTATATTTATTTGCATATTAACAT
AAAATACATCCTTGCATGCACCATCAATGCAAGGTCCTAAACCCTATATATTGGGTGTGT
CCCCTTTCCTATTCTACCAACCAAATTATATCATGTCATGTAAAAGCTACTCGTATAATG
TTAAAGTTGTCATACTTATGCTTAATGAGACAACAAATGAAGACGGGATCTTCATCCAAT
TAAGTTAATTAACAAGTAGTGAAATACCAATGATCATCTAAAAACTCTGAGTAAGTGATT
TGCATTGGTGAACCCTCCATCAATCATGAGATTCTGTCCACTCACATACCTCGCATCATC
ACTTGCAAGGAAGAGCACAGCATTAGCCACATCATTAGCAGTTAACTCTACCCCTTGCAA
GTTTGCATTCCTCCCAACAAAAGAACGAAAACCAGCCATTGCATCCTCAGTCCTCTCTTC
CTCAGGCAAATGAGCCA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC005661A_C01 KMC005661A_c01
         (557 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_175644.1| short chain alcohol dehydrogenase, putative; pr...   117  7e-26
gb|AAK38665.1| stem secoisolariciresinol dehydrogenase [Forsythi...    64  1e-09
ref|NP_190736.1| short-chain alcohol dehydrogenase-like protein;...    60  1e-08
sp|P50160|TS2_MAIZE SEX DETERMINATION PROTEIN TASSELSEED 2 gi|53...    59  3e-08
gb|AAB57737.1| short-chain alcohol dehydrogenase [Tripsacum dact...    59  3e-08

>ref|NP_175644.1| short chain alcohol dehydrogenase, putative; protein id:
           At1g52340.1, supported by cDNA: gi_20466273 [Arabidopsis
           thaliana] gi|25283121|pir||F96563 hypothetical protein
           F19K6.3 [imported] - Arabidopsis thaliana
           gi|12323116|gb|AAG51536.1|AC037424_1 short chain alcohol
           dehydrogenase, putative; 41546-43076 [Arabidopsis
           thaliana] gi|20466274|gb|AAM20454.1| short chain alcohol
           dehydrogenase, putative [Arabidopsis thaliana]
           gi|22651515|gb|AAL99237.1| short-chain
           dehydrogenase/reductase [Arabidopsis thaliana]
           gi|22651517|gb|AAL99238.1| short-chain
           dehydrogenase/reductase [Arabidopsis thaliana]
           gi|28059614|gb|AAO30075.1| short chain alcohol
           dehydrogenase, putative [Arabidopsis thaliana]
          Length = 285

 Score =  117 bits (294), Expect = 7e-26
 Identities = 56/73 (76%), Positives = 62/73 (84%)
 Frame = -3

Query: 555 AHLPEEERTEDAMAGFRSFVGRNANLQGVELTANDVANAVLFLASDDARYVSGQNLMIDG 376
           AHLPEEERTEDA  GFR+F   NANL+GVELT +DVANAVLFLASDD+RY+SG NLMIDG
Sbjct: 213 AHLPEEERTEDAFVGFRNFAAANANLKGVELTVDDVANAVLFLASDDSRYISGDNLMIDG 272

Query: 375 GFTNANHLLRVFR 337
           GFT  NH  +VFR
Sbjct: 273 GFTCTNHSFKVFR 285

>gb|AAK38665.1| stem secoisolariciresinol dehydrogenase [Forsythia x intermedia]
          Length = 277

 Score = 64.3 bits (155), Expect = 1e-09
 Identities = 29/58 (50%), Positives = 41/58 (70%)
 Frame = -3

Query: 510 FRSFVGRNANLQGVELTANDVANAVLFLASDDARYVSGQNLMIDGGFTNANHLLRVFR 337
           F + +    NL+G +    DVANA L+LASD+A+YVSG NL IDGGF+  N +++VF+
Sbjct: 216 FENVINFAGNLKGPKFNVEDVANAALYLASDEAKYVSGHNLFIDGGFSVCNSVIKVFQ 273

>ref|NP_190736.1| short-chain alcohol dehydrogenase-like protein; protein id:
           At3g51680.1 [Arabidopsis thaliana]
           gi|11250813|pir||T46064 short-chain alcohol
           dehydrogenase-like protein - Arabidopsis thaliana
           gi|6580150|emb|CAB63154.1| short-chain alcohol
           dehydrogenase-like protein [Arabidopsis thaliana]
          Length = 303

 Score = 60.5 bits (145), Expect = 1e-08
 Identities = 28/58 (48%), Positives = 40/58 (68%)
 Frame = -3

Query: 528 EDAMAGFRSFVGRNANLQGVELTANDVANAVLFLASDDARYVSGQNLMIDGGFTNANH 355
           +D +     FV   ANL+G  L AND+A A L+LASD+++YV+G NL++DGG T A +
Sbjct: 242 DDDVEEMEEFVRSLANLKGETLRANDIAEAALYLASDESKYVNGHNLVVDGGVTTARN 299

>sp|P50160|TS2_MAIZE SEX DETERMINATION PROTEIN TASSELSEED 2 gi|539065|pir||A47542
           short-chain alcohol dehydrogenase (EC 1.1.1.-) - maize
           gi|393184|gb|AAC37345.1| alcohol dehydrogenase
          Length = 336

 Score = 59.3 bits (142), Expect = 3e-08
 Identities = 30/65 (46%), Positives = 42/65 (64%)
 Frame = -3

Query: 543 EEERTEDAMAGFRSFVGRNANLQGVELTANDVANAVLFLASDDARYVSGQNLMIDGGFTN 364
           E E+ E+ + G        A L+G  L   D+A AVLFLASD+ARY+SG NL++DGG T 
Sbjct: 277 EVEKMEEVVRGL-------ATLKGPTLRPRDIAEAVLFLASDEARYISGHNLVVDGGVTT 329

Query: 363 ANHLL 349
           + +L+
Sbjct: 330 SRNLI 334

>gb|AAB57737.1| short-chain alcohol dehydrogenase [Tripsacum dactyloides]
          Length = 331

 Score = 59.3 bits (142), Expect = 3e-08
 Identities = 30/65 (46%), Positives = 42/65 (64%)
 Frame = -3

Query: 543 EEERTEDAMAGFRSFVGRNANLQGVELTANDVANAVLFLASDDARYVSGQNLMIDGGFTN 364
           E E+ E+ + G        A L+G  L   D+A AVLFLASD+ARY+SG NL++DGG T 
Sbjct: 272 EVEKMEEVVRGL-------ATLKGPTLRPRDIAEAVLFLASDEARYISGHNLVVDGGVTT 324

Query: 363 ANHLL 349
           + +L+
Sbjct: 325 SRNLI 329

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 464,882,764
Number of Sequences: 1393205
Number of extensions: 9616692
Number of successful extensions: 24620
Number of sequences better than 10.0: 1102
Number of HSP's better than 10.0 without gapping: 23076
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24351
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 19808345223
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFB070a02_f BP039054 1 532 2 MWL048a06_f AV769381 58 397 3 MPDL075c01_f AV780354 76 557

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