>KMC005634A_C01 KMC005634A_c01
AAGGCAGCAAAAAATCCCAAGGGAAACTTCTGAATATACAGTCAACCAGTGAGAATATAT
TTGAATATAACTTTGACAAAACAGCATTATGATTACAAGCTGACAATCACACTTTATAAT
GATCAATATTCAGGAGTAAAGAGATGTGAGATGAAGATTGATACAAGGAAGGAAGGCTTT
ACAAAGTTGTCCTCAGAAATGGTACCACAAAATAAAGAATTGCCAGGAACAGTGCAATCT
GGTATACATCTTGCTGAAAGCTGTCGTCCCTCTGGAAGAACTTGGTTGGATCGCCAGAAG
GATCTGGATAAATCTCCAAGTTCTCTTTCTCGGATTCAAAATTACTACTCATATTCTTAG
CACTATCACCCAAGTCTTTTACAGATTCAACAGGGGCAAATAGAGCACGTGATGTCCAGA
AAAATGCCTGAGCACTAGCCGATGAAGCTGACAAGTCCCAGAACTCTTCATGCTCAATGA
CTTGTCCACTGATTTGGTTAAGAGTAAATTTGGAAGTGATTCGTAGTATCACATCTCCTC
CCACCCTAGCAATAAAAGATTTAGGCTTCCCTTTCATTGTCCA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC005634A_C01 KMC005634A_c01
         (583 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_176702.2| unknown protein; protein id: At1g65230.1, suppo...   181  6e-45
dbj|BAB90296.1| P0470A12.24 [Oryza sativa (japonica cultivar-gro...   150  1e-35
pir||D96676 hypothetical protein T23K8.14 [imported] - Arabidops...   129  2e-29
dbj|BAA34091.1| 110kDa xylanase (XynE) [Aeromonas punctata]            41  0.010

>ref|NP_176702.2| unknown protein; protein id: At1g65230.1, supported by cDNA:
           gi_20466567 [Arabidopsis thaliana]
           gi|20466568|gb|AAM20601.1| unknown protein [Arabidopsis
           thaliana] gi|23198128|gb|AAN15591.1| unknown protein
           [Arabidopsis thaliana]
          Length = 286

 Score =  181 bits (459), Expect = 6e-45
 Identities = 86/134 (64%), Positives = 110/134 (81%)
 Frame = -1

Query: 583 WTMKGKPKSFIARVGGDVILRITSKFTLNQISGQVIEHEEFWDLSASSASAQAFFWTSRA 404
           WT+KGKPKS +A V GD+I+++ S+FTLNQISGQV EHEE WDLS+SS  AQA+FWTSR 
Sbjct: 154 WTVKGKPKSILAAVSGDLIVKVKSEFTLNQISGQVFEHEESWDLSSSSPIAQAYFWTSRR 213

Query: 403 LFAPVESVKDLGDSAKNMSSNFESEKENLEIYPDPSGDPTKFFQRDDSFQQDVYQIALFL 224
           LFA  ES KD+ D  K++++N  + KE+ +IY DP+ DP KFFQRD+SF++DVYQIALFL
Sbjct: 214 LFAASESAKDVADVTKDLTANLTTRKEDTDIYRDPT-DPNKFFQRDNSFERDVYQIALFL 272

Query: 223 AILYFVVPFLRTTL 182
           AI+YFVV FL+ T+
Sbjct: 273 AIVYFVVQFLKNTI 286

>dbj|BAB90296.1| P0470A12.24 [Oryza sativa (japonica cultivar-group)]
          Length = 315

 Score =  150 bits (379), Expect = 1e-35
 Identities = 81/137 (59%), Positives = 99/137 (72%), Gaps = 3/137 (2%)
 Frame = -1

Query: 583 WTMKGKPKSFI-ARVGGDVILRITSKFTLNQISGQVIEHEEFWDLSASSASAQAFFWTSR 407
           WT +GKPK+ I A +GGD+I+ +TS+F LNQISGQV+E              QA+FW SR
Sbjct: 193 WTFRGKPKNPIFATIGGDLIVSVTSQFVLNQISGQVLE--------------QAYFWLSR 238

Query: 406 ALFAPVESVKDLGDSAKNMSSNFESEK-ENLEIYPDPSGDPTKFFQR-DDSFQQDVYQIA 233
             F+ VE+ KD  ++AK  +S   S+K ENLE+YPDPSGDPTKFFQR DD   QDVYQIA
Sbjct: 239 RAFSTVEAGKDTIEAAKGTASRLSSKKDENLEVYPDPSGDPTKFFQRPDDGLNQDVYQIA 298

Query: 232 LFLAILYFVVPFLRTTL 182
           LFLA+LYF+V FLRTTL
Sbjct: 299 LFLAVLYFIVQFLRTTL 315

>pir||D96676 hypothetical protein T23K8.14 [imported] - Arabidopsis thaliana
           gi|4646202|gb|AAD26875.1|AC007230_9 ESTs gb|H76289 and
           gb|H76537 come from this gene. [Arabidopsis thaliana]
          Length = 218

 Score =  129 bits (325), Expect = 2e-29
 Identities = 61/101 (60%), Positives = 79/101 (77%)
 Frame = -1

Query: 583 WTMKGKPKSFIARVGGDVILRITSKFTLNQISGQVIEHEEFWDLSASSASAQAFFWTSRA 404
           WT+KGKPKS +A V GD+I+++ S+FTLNQISGQV EHEE WDLS+SS  AQA+FWTSR 
Sbjct: 115 WTVKGKPKSILAAVSGDLIVKVKSEFTLNQISGQVFEHEESWDLSSSSPIAQAYFWTSRR 174

Query: 403 LFAPVESVKDLGDSAKNMSSNFESEKENLEIYPDPSGDPTK 281
           LFA  ES KD+ D  K++++N  + KE+ +IY DP+ DP K
Sbjct: 175 LFAASESAKDVADVTKDLTANLTTRKEDTDIYRDPT-DPNK 214

>dbj|BAA34091.1| 110kDa xylanase (XynE) [Aeromonas punctata]
          Length = 941

 Score = 41.2 bits (95), Expect = 0.010
 Identities = 22/70 (31%), Positives = 39/70 (55%), Gaps = 1/70 (1%)
 Frame = -1

Query: 466 EFWDLSASSASAQA-FFWTSRALFAPVESVKDLGDSAKNMSSNFESEKENLEIYPDPSGD 290
           + W +  S A+A A   W  + L+     + D+ D  ++  S+   E++++EI+ DPS D
Sbjct: 781 DVWVIGNSGATATAQLLWDEKYLYV----LADVKDPLRSKLSSNAHEQDSIEIFIDPSKD 836

Query: 289 PTKFFQRDDS 260
            T F+Q DD+
Sbjct: 837 QTTFYQEDDA 846

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 495,691,685
Number of Sequences: 1393205
Number of extensions: 10562530
Number of successful extensions: 30614
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 29540
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30600
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21712003912
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFB047b11_f BP037405 1 493 2 SPD081d12_f BP050465 1 546 3 MWM116f10_f AV766595 5 354 4 MFB065h03_f BP038748 8 455 5 MPDL060d10_f AV779551 13 387 6 MF080h02_f BP032537 38 574 7 MFB032c10_f BP036338 44 583 8 MFB087d09_f BP040360 52 123 9 MFB061d04_f BP038425 64 453

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