Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005598A_C01 KMC005598A_c01
(1244 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAN07899.1| 20S proteasome alpha 6 subunit [Nicotiana bentham... 455 e-127
ref|NP_175158.1| 20S proteasome alpha subunit F2 (PAF2); protein... 443 e-123
gb|AAM61575.1| 20S proteasome subunit PAF1 [Arabidopsis thaliana] 443 e-123
pir||T51974 C 3.4.25.1 proteasome endopeptidase complex () chain... 442 e-123
ref|NP_199093.1| 20S proteasome alpha subunit F1 (PAF1); protein... 442 e-123
>gb|AAN07899.1| 20S proteasome alpha 6 subunit [Nicotiana benthamiana]
Length = 274
Score = 455 bits (1171), Expect = e-127
Identities = 230/280 (82%), Positives = 252/280 (89%), Gaps = 1/280 (0%)
Frame = -2
Query: 1165 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 986
MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHV+LACVNKANSELSSHQ
Sbjct: 1 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVILACVNKANSELSSHQ 60
Query: 985 KKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVHLADKAQVCT 806
KKIFKVDDHIGVAIAGLTADGRVLSRYMR+ECINYSY YESPLPVGRLVV LADKAQVCT
Sbjct: 61 KKIFKVDDHIGVAIAGLTADGRVLSRYMRNECINYSYAYESPLPVGRLVVQLADKAQVCT 120
Query: 805 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFV 626
QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFE F+
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFETFM 180
Query: 625 SSSREDLIKDALIATRESLQGEKLRSSVCTIAVVGVGEPFHILDQETVQQMIDAFEIVKE 446
SSRE L+KDAL A RE+LQGEKL S+ CT+AVVGVGE FH+LD+ET+Q +I+ FEI E
Sbjct: 181 DSSRETLLKDALFALRETLQGEKLTSTNCTVAVVGVGEAFHMLDKETIQALINEFEIAGE 240
Query: 445 DEAPPAEPEQA-AEQGAAADQGTGAGQGSAENQGGSPMDI 329
EAP A P++A +++ AAA + +G+ +QG +PMDI
Sbjct: 241 -EAPAAAPDEAGSDEPAAAPE-----EGAPADQGAAPMDI 274
>ref|NP_175158.1| 20S proteasome alpha subunit F2 (PAF2); protein id: At1g47250.1,
supported by cDNA: gi_16323090, supported by cDNA:
gi_3421093 [Arabidopsis thaliana]
gi|12229892|sp|O23712|PS12_ARATH Proteasome subunit alpha
type 1-2 (20S proteasome alpha subunit F2)
gi|25290067|pir||T51975 C 3.4.25.1 proteasome
endopeptidase complex () PAF2 [imported] - Arabidopsis
thaliana gi|3421094|gb|AAC32063.1| 20S proteasome subunit
PAF2 [Arabidopsis thaliana]
gi|12325400|gb|AAG52642.1|AC079677_6 20S proteasome
subunit PAF2; 11103-9423 [Arabidopsis thaliana]
gi|16323091|gb|AAL15280.1| At1g47250/F8G22_3 [Arabidopsis
thaliana] gi|22655336|gb|AAM98260.1| At1g47250/F8G22_3
[Arabidopsis thaliana]
Length = 277
Score = 443 bits (1140), Expect = e-123
Identities = 222/279 (79%), Positives = 248/279 (88%)
Frame = -2
Query: 1165 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 986
MFRNQYDTDVTTWSP GRLFQVEYAMEAVKQGSAAIGLRS++HVVLACVNKA SELSSHQ
Sbjct: 1 MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQ 60
Query: 985 KKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVHLADKAQVCT 806
+KIFKVDDHIGVAIAGLTADGRVLSRYMRSE IN+S+TYESPLPVGRLVVHLADKAQVCT
Sbjct: 61 RKIFKVDDHIGVAIAGLTADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKAQVCT 120
Query: 805 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFV 626
QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLER+FE+F
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERKFESFQ 180
Query: 625 SSSREDLIKDALIATRESLQGEKLRSSVCTIAVVGVGEPFHILDQETVQQMIDAFEIVKE 446
SS+EDLIKDA++A RE+LQGE L+SS+CT++V+GV EPFH LDQE++Q++ID FE V E
Sbjct: 181 ESSKEDLIKDAIMAIRETLQGETLKSSLCTVSVLGVDEPFHFLDQESIQKVIDTFEKVPE 240
Query: 445 DEAPPAEPEQAAEQGAAADQGTGAGQGSAENQGGSPMDI 329
+E E E A + A GTG QG + +Q +PM+I
Sbjct: 241 EEEDAGEGE-AEPEAAPGAAGTGE-QGGSGDQDVAPMEI 277
>gb|AAM61575.1| 20S proteasome subunit PAF1 [Arabidopsis thaliana]
Length = 278
Score = 443 bits (1139), Expect = e-123
Identities = 220/279 (78%), Positives = 244/279 (86%)
Frame = -2
Query: 1165 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 986
MFRNQYDTDVTTWSP GRLFQVEYAMEAVKQGSAAIGLRS++HVVLACVNKA SELSSHQ
Sbjct: 1 MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQ 60
Query: 985 KKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVHLADKAQVCT 806
+KIFKVDDHIGVAIAGLTADGRVLSRYMRSE IN+S+TYESPLPVGRLVVHLADKAQVCT
Sbjct: 61 RKIFKVDDHIGVAIAGLTADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKAQVCT 120
Query: 805 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFV 626
QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFE+F
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFESFG 180
Query: 625 SSSREDLIKDALIATRESLQGEKLRSSVCTIAVVGVGEPFHILDQETVQQMIDAFEIVKE 446
SSREDLIKDA++A RE+LQGE L+SS+CT+A++GV EPFH LDQE +Q++ID FE V E
Sbjct: 181 DSSREDLIKDAILAVRETLQGETLKSSLCTVAILGVDEPFHFLDQEAIQKVIDTFEKVPE 240
Query: 445 DEAPPAEPEQAAEQGAAADQGTGAGQGSAENQGGSPMDI 329
+E E + + A A G G A +Q +PM++
Sbjct: 241 EEEAEGEAGEGEAEAAGAAPAEQGG-GGAGDQDVAPMEM 278
>pir||T51974 C 3.4.25.1 proteasome endopeptidase complex () chain PAF1 [imported]
- Arabidopsis thaliana gi|3421092|gb|AAC32062.1| 20S
proteasome subunit PAF1 [Arabidopsis thaliana]
Length = 278
Score = 442 bits (1138), Expect = e-123
Identities = 220/279 (78%), Positives = 244/279 (86%)
Frame = -2
Query: 1165 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 986
MFRNQYDTDVTTWSP GRLFQVEYAMEAVKQGSAAIGLRS++HVVLACVNKA SELSSHQ
Sbjct: 1 MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQ 60
Query: 985 KKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVHLADKAQVCT 806
+KIFKVDDHIGVAIAGLTADGRVLSRYMRSE IN+S+TYESPLPVGRLVVHLADKAQVCT
Sbjct: 61 RKIFKVDDHIGVAIAGLTADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKAQVCT 120
Query: 805 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFV 626
QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFE+F
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFESFG 180
Query: 625 SSSREDLIKDALIATRESLQGEKLRSSVCTIAVVGVGEPFHILDQETVQQMIDAFEIVKE 446
SSREDLIKDA++A RE+LQGE L+SS+CT+A++GV EPFH LDQE +Q++ID FE V E
Sbjct: 181 DSSREDLIKDAILAVRETLQGETLKSSLCTVAILGVDEPFHFLDQEAIQKVIDTFEKVPE 240
Query: 445 DEAPPAEPEQAAEQGAAADQGTGAGQGSAENQGGSPMDI 329
+E E + + A A G G A +Q +PM++
Sbjct: 241 EEEGEGEAGEGEAEAAGAAPAEQGG-GGAGDQDVAPMEM 278
>ref|NP_199093.1| 20S proteasome alpha subunit F1 (PAF1); protein id: At5g42790.1,
supported by cDNA: 12455., supported by cDNA:
gi_14030712, supported by cDNA: gi_16648703 [Arabidopsis
thaliana] gi|17380545|sp|P34066|PS11_ARATH Proteasome
subunit alpha type 1-1 (20S proteasome alpha subunit F1)
(Proteasome 30 kDa subunit) gi|541889|pir||S39900
multicatalytic endopeptidase complex 30K chain homolog -
Arabidopsis thaliana gi|166830|gb|AAA16326.1| proteasome
gi|10177282|dbj|BAB10635.1| 20S proteasome subunit PAF1
[Arabidopsis thaliana]
gi|14030713|gb|AAK53031.1|AF375447_1 AT5g42790/MJB21_17
[Arabidopsis thaliana] gi|16648704|gb|AAL25544.1|
AT5g42790/MJB21_17 [Arabidopsis thaliana]
gi|21360479|gb|AAM47355.1| AT5g42790/MJB21_17
[Arabidopsis thaliana]
Length = 278
Score = 442 bits (1136), Expect = e-123
Identities = 222/282 (78%), Positives = 247/282 (86%), Gaps = 3/282 (1%)
Frame = -2
Query: 1165 MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 986
MFRNQYDTDVTTWSP GRLFQVEYAMEAVKQGSAAIGLRS++HVVLACVNKA SELSSHQ
Sbjct: 1 MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQ 60
Query: 985 KKIFKVDDHIGVAIAGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVHLADKAQVCT 806
+KIFKVDDHIGVAIAGLTADGRVLSRYMRSE IN+S+TYESPLPVGRLVVHLADKAQVCT
Sbjct: 61 RKIFKVDDHIGVAIAGLTADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKAQVCT 120
Query: 805 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFV 626
QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFE+F
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFESFG 180
Query: 625 SSSREDLIKDALIATRESLQGEKLRSSVCTIAVVGVGEPFHILDQETVQQMIDAFEIVKE 446
SSREDLIKDA++A RE+LQGE L+SS+CT+A++GV EPFH LDQE +Q++ID FE V E
Sbjct: 181 DSSREDLIKDAILAVRETLQGETLKSSLCTVAILGVDEPFHFLDQEAIQKVIDTFEKVPE 240
Query: 445 D---EAPPAEPEQAAEQGAAADQGTGAGQGSAENQGGSPMDI 329
+ E E E A + A A++G G A +Q +PM++
Sbjct: 241 EEEGEGEAGEGEAEAAEAAPAERGGGV----AGDQDVAPMEM 278
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,122,449,607
Number of Sequences: 1393205
Number of extensions: 27337844
Number of successful extensions: 146418
Number of sequences better than 10.0: 2350
Number of HSP's better than 10.0 without gapping: 105162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137013
length of database: 448,689,247
effective HSP length: 126
effective length of database: 273,145,417
effective search space used: 78665880096
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
