>KMC005514A_C01 KMC005514A_c01
catctgcccatcttcatttcgtagaaaagattagatttttcaagtgcttgagagttttct
gtctctataatctggtacacTCGAATTCTGGTGAATGGGTATGTGCTGTTTGGTTTAGAA
TCAAAGACCCACCTTCTGTTTGGTTAAACAGATCAGAAGAAATTTGTGAGAATAGTTGAC
ATGTGAGAGAAATGTCAGAAATTTAAGCATTTCAATAAACAAAAATTGGAACTTGGGGTT
TTGGTTAATGTGAGGGTGTGAGCTGTTTACACTCAGAATATTGCAAGTTTGGATTTGTCT
GTTGTTGCATCACCACCACTGATAATTGCAATTTTGATTTTTATAAATATTTCATTTTTA
GCCAGAAGCTGAAGCTATGGTGAATCTGGGATTGTTTTCTTCGAATAAGTCTGTGAGTTC
AGTGAAGGTAACCTTTCGGATACCATATTTTACCCAATGGGGTCAGAGCTTGCTGGTGTG
TGGTTCTGTGCCATTGCTTGGTTCTGGGAATGTGAAGAAGGGTGTGTTGCTCAGTCCATC
TCCTCAAGGGTCTGAGCTCGTGTGGAGCGGAAGCATCACAGTGCCAAGAGGGTTCCAATG
CCAGTATAGCTATTATGTTGTAGATGATAACAAAAATATTCTGAGATGGGAGATGGGTAA
GAAGCGCGAATTGAACCTTCCTGAAGGTCTTCAGAATGGTCAGGAGGTTGAGTTCCGTGA
TCTTTGGCAGACTGGCTCAGATGCTCTCCCTTTCAGAAGCGCTTTCAAAGATGTTGTTTT
TCGGCAAAGCTGGGATTCGTGCATCAC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC005514A_C01 KMC005514A_c01
         (807 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAL91204.1| 4-alpha-glucanotransferase [Arabidopsis thaliana]      181  1e-44
ref|NP_671950.1| similar to 4-alpha-glucanotransferase; protein ...   181  1e-44
dbj|BAC22431.1| putative 4-alpha-glucanotransferase [Oryza sativ...   173  2e-42
gb|EAA34371.1| hypothetical protein [Neurospora crassa]                54  2e-06
gb|ZP_00017646.1| hypothetical protein [Chloroflexus aurantiacus]      54  2e-06

>gb|AAL91204.1| 4-alpha-glucanotransferase [Arabidopsis thaliana]
          Length = 955

 Score =  181 bits (459), Expect = 1e-44
 Identities = 88/143 (61%), Positives = 107/143 (74%), Gaps = 3/143 (2%)
 Frame = +2

Query: 377 MVNLGLFSSNKSVSS---VKVTFRIPYFTQWGQSLLVCGSVPLLGSGNVKKGVLLSPSPQ 547
           M+NLG  S + S SS   V ++F IPYFT WG+SLLVCGS P LGSGNVKKG+LL PS Q
Sbjct: 1   MMNLGSLSLSTSKSSKPMVSISFWIPYFTHWGESLLVCGSAPGLGSGNVKKGLLLKPSQQ 60

Query: 548 GSELVWSGSITVPRGFQCQYSYYVVDDNKNILRWEMGKKRELNLPEGLQNGQEVEFRDLW 727
             +L+WSGS++VP GF   Y YYVVDD+K++LR E G KR+L +PE L  G+ V  RDLW
Sbjct: 61  DDQLIWSGSVSVPPGFSSDYCYYVVDDSKSVLRSEFGMKRKLVVPETLTGGESVHLRDLW 120

Query: 728 QTGSDALPFRSAFKDVVFRQSWD 796
           Q+G  ALPFRSAFKDV+F  S+D
Sbjct: 121 QSGDQALPFRSAFKDVIFHHSFD 143

>ref|NP_671950.1| similar to 4-alpha-glucanotransferase; protein id: At2g40835.1
           [Arabidopsis thaliana]
          Length = 234

 Score =  181 bits (459), Expect = 1e-44
 Identities = 88/143 (61%), Positives = 107/143 (74%), Gaps = 3/143 (2%)
 Frame = +2

Query: 377 MVNLGLFSSNKSVSS---VKVTFRIPYFTQWGQSLLVCGSVPLLGSGNVKKGVLLSPSPQ 547
           M+NLG  S + S SS   V ++F IPYFT WG+SLLVCGS P LGSGNVKKG+LL PS Q
Sbjct: 1   MMNLGSLSLSTSKSSKPMVSISFWIPYFTHWGESLLVCGSAPGLGSGNVKKGLLLKPSQQ 60

Query: 548 GSELVWSGSITVPRGFQCQYSYYVVDDNKNILRWEMGKKRELNLPEGLQNGQEVEFRDLW 727
             +L+WSGS++VP GF   Y YYVVDD+K++LR E G KR+L +PE L  G+ V  RDLW
Sbjct: 61  DDQLIWSGSVSVPPGFSSDYCYYVVDDSKSVLRSEFGMKRKLVVPETLTGGESVHLRDLW 120

Query: 728 QTGSDALPFRSAFKDVVFRQSWD 796
           Q+G  ALPFRSAFKDV+F  S+D
Sbjct: 121 QSGDQALPFRSAFKDVIFHHSFD 143

>dbj|BAC22431.1| putative 4-alpha-glucanotransferase [Oryza sativa (japonica
           cultivar-group)]
          Length = 922

 Score =  173 bits (439), Expect = 2e-42
 Identities = 84/141 (59%), Positives = 108/141 (76%)
 Frame = +2

Query: 368 AEAMVNLGLFSSNKSVSSVKVTFRIPYFTQWGQSLLVCGSVPLLGSGNVKKGVLLSPSPQ 547
           A+ M NL   S  KS+++V + F++PY+TQWGQSLL+ GS P LGS NVK+G+ LSP  Q
Sbjct: 3   AKRMTNL---SGKKSLNTVTLVFKLPYYTQWGQSLLIAGSEPALGSWNVKQGLSLSPVHQ 59

Query: 548 GSELVWSGSITVPRGFQCQYSYYVVDDNKNILRWEMGKKRELNLPEGLQNGQEVEFRDLW 727
           G+EL+WSG ++V  GF CQY+YYVVDDNKN+LR E G+KR+L LPEG+Q+G  VE RD W
Sbjct: 60  GNELIWSGRVSVATGFTCQYNYYVVDDNKNVLRSESGEKRKLVLPEGVQDGDVVEIRDWW 119

Query: 728 QTGSDALPFRSAFKDVVFRQS 790
           Q  S+AL  RSAFK+V+F  S
Sbjct: 120 QDASEALFLRSAFKNVIFNGS 140

>gb|EAA34371.1| hypothetical protein [Neurospora crassa]
          Length = 465

 Score = 54.3 bits (129), Expect = 2e-06
 Identities = 29/100 (29%), Positives = 50/100 (50%), Gaps = 1/100 (1%)
 Frame = +2

Query: 416 SSVKVTFRIPYFTQWGQSLLVCGSVPLLGSGNVKKGVLLSPSP-QGSELVWSGSITVPRG 592
           ++V VTF     T +G+S+ + GS+  LGS +   GV LS S    S  +W+ ++++P G
Sbjct: 362 ATVAVTFNHLASTSYGESIKIVGSISQLGSWSASSGVALSASQYTTSNPLWTATVSLPAG 421

Query: 593 FQCQYSYYVVDDNKNILRWEMGKKRELNLPEGLQNGQEVE 712
            + +Y +  V    + + WE    R   +P+       VE
Sbjct: 422 TKFEYKFVKVSSEGSAVTWESDPNRSYTVPQSCAESVAVE 461

>gb|ZP_00017646.1| hypothetical protein [Chloroflexus aurantiacus]
          Length = 575

 Score = 54.3 bits (129), Expect = 2e-06
 Identities = 30/89 (33%), Positives = 49/89 (54%)
 Frame = +2

Query: 416 SSVKVTFRIPYFTQWGQSLLVCGSVPLLGSGNVKKGVLLSPSPQGSELVWSGSITVPRGF 595
           ++V VTF +   T WGQ++ V G++P LG+ N  + V LS +   +  VWSG++ +P   
Sbjct: 479 ATVAVTFNVNATTYWGQNVFVVGNIPQLGNWNPAQAVPLSAA---TYPVWSGTVNLPANT 535

Query: 596 QCQYSYYVVDDNKNILRWEMGKKRELNLP 682
             +Y  Y+  D  N++ WE    R +  P
Sbjct: 536 TIEYK-YIKRDGSNVV-WECCNNRVITTP 562

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 712,584,303
Number of Sequences: 1393205
Number of extensions: 16391597
Number of successful extensions: 41749
Number of sequences better than 10.0: 51
Number of HSP's better than 10.0 without gapping: 39617
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 41710
length of database: 448,689,247
effective HSP length: 121
effective length of database: 280,111,442
effective search space used: 41176381974
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPDL077a11_f AV780446 1 525 2 MPDL020f01_f AV777518 260 570 3 MFBL040b07_f BP043271 264 807

---