>KMC005510A_C01 KMC005510A_c01
aaaacTAATCATAATTAGATATTGCATCTCTAATTCAAATTAAGGCTAGAAACCAAAACA
AAGGCTCGTTATTCGAGTAAAAGTATTCCATAACTCTTGTTTCTCTACTCATAAGTTGAT
AGTTAAAAATACCAACATTGCCACTAAGCCAAATTATAAAAAGGGTGCATGTGACATTGG
ATTTCATAAAAAATGGCCGGATAAACCATTCTTCATTTATGCAATATGCCCGGGCAAGGT
AAGAATCTGGACATGCTTCTTCCGCCTAAGCTCATGAGGTACCGGTCCAAAAACTCTGGT
CCCAATAGGCTGGCCTTGCTTGTCAACAAGAACAACAGCATTGTCATCAAACCTGACCTC
GCCGCCATCACATCTCCCCTTCTGCATTGCTGCACGCACAACAACCCCATATACCACCTT
TCCTTTCTTCACTTTTCCATTAGGATGTGCTTCCTTCACTGATGCTACTATAGTGTCACC
TAATCTTGCCCCTTTCTTCCCTTTCAATGCCTGTATGCACATCACCTTTTTAGCCCCAGA
ATTGTCCACAACCTTGAGAACAGTCCTCATTTGTATGAAGGTGCGTTGCTGCTGAGAAAA
ATAATTGTTGTGTGTCATCTCATGTGCTGAACCAAGGGAACTGTTGCTTAATCCGCTGAA
CAGAGAACGGCCCACACGAGAACATCTGGAAGCAAAACTTGCAGCCATCTTCGGAAGTTG
CAGTCTTTTTTATTCCCTGTCAAAACTCAAAACTTCACAACCTTGTCCAGTGGCGGATCC
AGGACCCCAGGGTCAGGGGGTTCAAATTTGTTTtactaaaaatttgtccatgacgtttct
gggaaaattaaacttgagaagagaaaggtgaactggactggtccagtggtggt

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC005510A_C01 KMC005510A_c01
         (893 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAM13095.1| unknown protein [Arabidopsis thaliana] gi|2213619...   234  1e-60
ref|NP_199428.2| 50S ribosomal protein L14; protein id: At5g4616...   234  1e-60
gb|AAL60450.1|AF402991_1 HUELLENLOS-like protein [Oryza sativa]       217  2e-55
dbj|BAB08261.1| 50S ribosomal protein L14 [Arabidopsis thaliana]      194  2e-48
gb|AAF79479.1|AC022492_23 F1L3.27 [Arabidopsis thaliana]              181  1e-44

>gb|AAM13095.1| unknown protein [Arabidopsis thaliana] gi|22136190|gb|AAM91173.1|
           unknown protein [Arabidopsis thaliana]
          Length = 172

 Score =  234 bits (598), Expect = 1e-60
 Identities = 128/172 (74%), Positives = 142/172 (82%), Gaps = 9/172 (5%)
 Frame = -3

Query: 708 MAASFASRCSRVGRSLFSGLSN-SSLGSAHEMTHNNYFSQQQR-TFIQMRTVLKVVDNSG 535
           MAA+FASR +R GRSL  GL+N  S+ S++ M + +  SQQQR TFIQM TVLKVVDNSG
Sbjct: 1   MAAAFASRLTRGGRSLLGGLNNVGSMNSSNGMMNESILSQQQRRTFIQMGTVLKVVDNSG 60

Query: 534 AKKVMCIQALKGKKGARLGDTIVASVKEAHPNGKVKKGKVVYGVVVRAAMQKGRCDGGEV 355
           AKKVMCIQALKGKKGARLGDTIVASVKEA PNGKVKKG VVYGVVVRAAMQ+GR DG EV
Sbjct: 61  AKKVMCIQALKGKKGARLGDTIVASVKEAMPNGKVKKGAVVYGVVVRAAMQRGRVDGSEV 120

Query: 354 RFDDNAVVLVDKQG-------QPIGTRVFGPVPHELRRKKHVQILTLPGHIA 220
           RFDDNAVVLVD +        QPIGTRVFGPVPHELR+KKH++IL L  H+A
Sbjct: 121 RFDDNAVVLVDSKDKNTKTDRQPIGTRVFGPVPHELRKKKHLKILALAQHVA 172

>ref|NP_199428.2| 50S ribosomal protein L14; protein id: At5g46160.1, supported by
           cDNA: gi_16604451, supported by cDNA: gi_18140859
           [Arabidopsis thaliana] gi|16604452|gb|AAL24232.1|
           AT5g46160/MCL19_22 [Arabidopsis thaliana]
           gi|18140860|gb|AAL60452.1|AF402993_1 HUELLENLOS PARALOG
           [Arabidopsis thaliana]
          Length = 173

 Score =  234 bits (597), Expect = 1e-60
 Identities = 128/173 (73%), Positives = 142/173 (81%), Gaps = 10/173 (5%)
 Frame = -3

Query: 708 MAASFASRCSRVGRSLFSGLSNS-SLGSAHEMTHNNYFSQQQ--RTFIQMRTVLKVVDNS 538
           MAA+FASR +R GRSL  GL+N  S+ S++ M + +  SQQQ  RTFIQM TVLKVVDNS
Sbjct: 1   MAAAFASRLTRGGRSLLGGLNNGGSMNSSNGMMNESILSQQQQRRTFIQMGTVLKVVDNS 60

Query: 537 GAKKVMCIQALKGKKGARLGDTIVASVKEAHPNGKVKKGKVVYGVVVRAAMQKGRCDGGE 358
           GAKKVMCIQALKGKKGARLGDTIVASVKEA PNGKVKKG VVYGVVVRAAMQ+GR DG E
Sbjct: 61  GAKKVMCIQALKGKKGARLGDTIVASVKEAMPNGKVKKGAVVYGVVVRAAMQRGRVDGSE 120

Query: 357 VRFDDNAVVLVDKQG-------QPIGTRVFGPVPHELRRKKHVQILTLPGHIA 220
           VRFDDNAVVLVD +        QPIGTRVFGPVPHELR+KKH++IL L  H+A
Sbjct: 121 VRFDDNAVVLVDSKDKNTKTDRQPIGTRVFGPVPHELRKKKHLKILALAQHVA 173

>gb|AAL60450.1|AF402991_1 HUELLENLOS-like protein [Oryza sativa]
          Length = 170

 Score =  217 bits (552), Expect = 2e-55
 Identities = 111/169 (65%), Positives = 133/169 (78%), Gaps = 7/169 (4%)
 Frame = -3

Query: 708 MAASFASRCSRVGRSLFSGLSNSSLGSAHEM-------THNNYFSQQQRTFIQMRTVLKV 550
           MAA   S+CS VGR+L  GL N+  G+ +         +H +   QQ RTFIQMRT LKV
Sbjct: 1   MAAFLRSKCSSVGRTLMGGLGNNLFGAVNSSVETVSRPSHCDPIFQQIRTFIQMRTNLKV 60

Query: 549 VDNSGAKKVMCIQALKGKKGARLGDTIVASVKEAHPNGKVKKGKVVYGVVVRAAMQKGRC 370
           VDNSGAK+VMCIQ+L+GKKGARLGDTI+ SVKEA P GKVKKG VVYGVVVRAAM++GR 
Sbjct: 61  VDNSGAKRVMCIQSLRGKKGARLGDTIIGSVKEAQPRGKVKKGDVVYGVVVRAAMKRGRN 120

Query: 369 DGGEVRFDDNAVVLVDKQGQPIGTRVFGPVPHELRRKKHVQILTLPGHI 223
           DG E++FDDNA+VLV+ +G+ IGTRVFGPVPHELR+KKH++IL L  HI
Sbjct: 121 DGSEIQFDDNAIVLVNNKGELIGTRVFGPVPHELRKKKHLKILALAEHI 169

>dbj|BAB08261.1| 50S ribosomal protein L14 [Arabidopsis thaliana]
          Length = 124

 Score =  194 bits (492), Expect = 2e-48
 Identities = 101/124 (81%), Positives = 107/124 (85%), Gaps = 7/124 (5%)
 Frame = -3

Query: 570 MRTVLKVVDNSGAKKVMCIQALKGKKGARLGDTIVASVKEAHPNGKVKKGKVVYGVVVRA 391
           M TVLKVVDNSGAKKVMCIQALKGKKGARLGDTIVASVKEA PNGKVKKG VVYGVVVRA
Sbjct: 1   MGTVLKVVDNSGAKKVMCIQALKGKKGARLGDTIVASVKEAMPNGKVKKGAVVYGVVVRA 60

Query: 390 AMQKGRCDGGEVRFDDNAVVLVDKQG-------QPIGTRVFGPVPHELRRKKHVQILTLP 232
           AMQ+GR DG EVRFDDNAVVLVD +        QPIGTRVFGPVPHELR+KKH++IL L 
Sbjct: 61  AMQRGRVDGSEVRFDDNAVVLVDSKDKNTKTDRQPIGTRVFGPVPHELRKKKHLKILALA 120

Query: 231 GHIA 220
            H+A
Sbjct: 121 QHVA 124

>gb|AAF79479.1|AC022492_23 F1L3.27 [Arabidopsis thaliana]
          Length = 201

 Score =  181 bits (459), Expect = 1e-44
 Identities = 109/197 (55%), Positives = 134/197 (67%), Gaps = 34/197 (17%)
 Frame = -3

Query: 711 KMAASFASRCSRVGRSLFSGLSNSSLG----SAHEMTHNNYFSQQQ-RTFIQMRTVLKVV 547
           +MA + AS+ S+ GRSL  GL N+  G    S++ M + +  SQQQ RTFIQM T+LK V
Sbjct: 5   EMATALASKLSK-GRSLLGGLCNAFSGLMNSSSNGMMNGSILSQQQHRTFIQMGTILKCV 63

Query: 546 DNSGAKKVMCIQALKGKKGARLGDTIVASVKEAH------------PNGKVKKGKVVYGV 403
           DNS AK+VMCIQ+L+GKKGARLGD IV SVKEA+            P GKVKKG VVYGV
Sbjct: 64  DNSCAKEVMCIQSLRGKKGARLGDIIVGSVKEANPIVQKKVKKDAIPKGKVKKGMVVYGV 123

Query: 402 VVRAAMQKGRCDGGEVRFDDNAVVLV---DKQG--------------QPIGTRVFGPVPH 274
           VVRAAM KGR DG +V+FDDNA+V+V   +K+G              QP GTRVFGPVPH
Sbjct: 124 VVRAAMPKGRADGSQVKFDDNAIVVVGIKEKKGQNNSHGSKRKMEYNQPTGTRVFGPVPH 183

Query: 273 ELRRKKHVQILTLPGHI 223
           E+R +K ++IL+L  HI
Sbjct: 184 EMRLRKQLKILSLAQHI 200

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 806,513,541
Number of Sequences: 1393205
Number of extensions: 18633222
Number of successful extensions: 49185
Number of sequences better than 10.0: 282
Number of HSP's better than 10.0 without gapping: 46294
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 48893
length of database: 448,689,247
effective HSP length: 122
effective length of database: 278,718,237
effective search space used: 48775691475
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPDL058e08_f BP055638 1 440 2 GNf079c11 BP073193 5 173 3 MPDL070h08_f AV780109 21 506 4 MF011b10_f BP028793 29 179 5 MPDL019d04_f AV777457 49 468 6 MPDL083c07_f AV780813 50 579 7 MPDL075f01_f AV780373 63 488 8 MFB085h06_f BP040242 401 898

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