KMC005505A_c01
[Fasta Sequence]
[Nr Search]
[EST assemble image]
Fasta Sequence
>KMC005505A_C01 KMC005505A_c01
ttttttttttttttttttaggagttactctaggtgggacaaccacccatgttttagtttc
tttatcccaacggcggaatgATGGTCGTTAGATACTACAAAGTAGCAACATTTCTCGCAT
CTCTTGATTCTGTCATTTGATGAAGAAATGTTGGTTAGATAAACTAACTTCACCATTTAT
GAGAGCTTGTGTATAGAGCTCATGTAAAGGATTGTACCACATCTTTTAGAAATTCACAAT
TTAGCAACTGATAAACAGTTCTGTAATACACTTTATTCAAGTCACAAGGAGTTCAAAACG
GAGCATTCAAAGATCAAGATGATACCACATACATTCTAAATGCAAGTTCAAATAGAGATG
CTGTATGGAACTCCCTTTGCTGTGACCCCTGCCTCTGAAACTGGCTTCAGCAAGCTCATA
AGGCACAATCCCAGCACCATTTCTGTTCTTCCGGCTCTTATCTGCATTACTCAAATCAAT
GATCCCTTCAAGCTCCATCAACTTCCCTTGAAACTTTTCAAACGAAGCTTTGATAATTGG
ATCCTGTTCCCATGATGGCTCCACAGTTTGCCCGAGATACTCCTCATCAGATGAGTGGCT
TGACAAAATATCCAACACTGCCATTATAGTTGTGGCTTGAATCTGAGATGGGAAACACTT
GAGCAGTGTCACCTCTGGTTTGTCCAAGAAAAGTTCCCATTCAGGGTCAGAAGGGTCCTC
TGTTGGCATATTGTTTCTTGCAACAGTTGGTCTGTTGGGGAAATAGCCTGCATAAGAGTA
CTGTCCGAAGTTCACAGCTGCATGGTGCCCTGATGTTATCCACACAATGGTGG
Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005505A_C01 KMC005505A_c01
(833 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T11578 probable lipoxygenase (EC 1.13.11.12) CPRD46, drough... 239 5e-62
emb|CAA05277.1| loxc homologue [Lycopersicon pimpinellifolium] 228 8e-59
gb|AAD13306.1| lipoxygenase [Lycopersicon esculentum] 228 8e-59
pir||T07038 probable lipoxygenase (EC 1.13.11.12) Lox2 - tomato ... 228 8e-59
emb|CAA05270.1| loxc homologue [Lycopersicon hirsutum] 228 8e-59
>pir||T11578 probable lipoxygenase (EC 1.13.11.12) CPRD46, drought-inducible -
cowpea gi|1853970|dbj|BAA13542.1| CPRD46 protein [Vigna
unguiculata]
Length = 899
Score = 239 bits (609), Expect = 5e-62
Identities = 109/153 (71%), Positives = 131/153 (85%), Gaps = 4/153 (2%)
Frame = -3
Query: 831 TIVWITSGHHAAVNFGQYSYAGYFPNRPTVARNNMPTEDPSDPEWELFLDKPEVTLLKCF 652
TI W TSGHHAAVNFGQ+S+AGYFPNRPT+AR N+P EDPSDPEWELFL KPEVT+LKCF
Sbjct: 740 TIAWTTSGHHAAVNFGQFSFAGYFPNRPTIARTNLPLEDPSDPEWELFLQKPEVTMLKCF 799
Query: 651 PSQIQATTIMAVLDILSSHSSDEEYLGQTVEPSWEQDPIIKASFEKFQGKLMELEGIIDL 472
PSQ QATT++ VLD+LS+HS DEEY+G +VEP+WEQ+P +KA+FEKF+G+L ELEG ID
Sbjct: 800 PSQYQATTVITVLDVLSNHSPDEEYIGTSVEPAWEQEPRVKAAFEKFKGRLNELEGTIDE 859
Query: 471 SNADKSRKNRNGAGIVPYEL----AEASFRGRG 385
NAD ++KNRNGAG+VPYEL +EA G+G
Sbjct: 860 RNADLTKKNRNGAGVVPYELLKPTSEAGVTGKG 892
Score = 42.0 bits (97), Expect = 0.011
Identities = 26/60 (43%), Positives = 32/60 (53%)
Frame = -1
Query: 530 KLRLKSFKGS*WSLKGSLI*VMQIRAGRTEMVLGLCLMSLLKPVSEAGVTAKGVPYSISI 351
K + FKG L+G++ + G+ LLKP SEAGVT KGVPYSISI
Sbjct: 840 KAAFEKFKGRLNELEGTIDERNADLTKKNRNGAGVVPYELLKPTSEAGVTGKGVPYSISI 899
>emb|CAA05277.1| loxc homologue [Lycopersicon pimpinellifolium]
Length = 341
Score = 228 bits (581), Expect = 8e-59
Identities = 104/153 (67%), Positives = 126/153 (81%), Gaps = 4/153 (2%)
Frame = -3
Query: 831 TIVWITSGHHAAVNFGQYSYAGYFPNRPTVARNNMPTEDPSDPEWELFLDKPEVTLLKCF 652
TI+W+TSGHHAAVNFGQYSYAGYFPNRPT+AR+ MPTEDP+D EWE FL+KPE LLKCF
Sbjct: 182 TIIWVTSGHHAAVNFGQYSYAGYFPNRPTIARSKMPTEDPTDEEWEDFLNKPEEALLKCF 241
Query: 651 PSQIQATTIMAVLDILSSHSSDEEYLGQTVEPSWEQDPIIKASFEKFQGKLMELEGIIDL 472
PSQ+QAT ++AVLD+LS+HS DEEY+G +EP W+ +P+I A+FE F GKL ELEGIID
Sbjct: 242 PSQLQATKVIAVLDVLSNHSPDEEYIGTNIEPFWKDEPVINAAFEVFSGKLKELEGIIDA 301
Query: 471 SNADKSRKNRNGAGIVPYEL----AEASFRGRG 385
NAD + KNRNGAG++PYEL +E G+G
Sbjct: 302 RNADCNLKNRNGAGVMPYELLKPFSEPGITGKG 334
Score = 38.5 bits (88), Expect = 0.12
Identities = 18/27 (66%), Positives = 20/27 (73%)
Frame = -1
Query: 431 GLCLMSLLKPVSEAGVTAKGVPYSISI 351
G+ LLKP SE G+T KGVPYSISI
Sbjct: 315 GVMPYELLKPFSEPGITGKGVPYSISI 341
>gb|AAD13306.1| lipoxygenase [Lycopersicon esculentum]
Length = 340
Score = 228 bits (581), Expect = 8e-59
Identities = 104/153 (67%), Positives = 126/153 (81%), Gaps = 4/153 (2%)
Frame = -3
Query: 831 TIVWITSGHHAAVNFGQYSYAGYFPNRPTVARNNMPTEDPSDPEWELFLDKPEVTLLKCF 652
TI+W+TSGHHAAVNFGQYSYAGYFPNRPT+AR+ MPTEDP+D EWE FL+KPE LLKCF
Sbjct: 181 TIIWVTSGHHAAVNFGQYSYAGYFPNRPTIARSKMPTEDPTDEEWEDFLNKPEEALLKCF 240
Query: 651 PSQIQATTIMAVLDILSSHSSDEEYLGQTVEPSWEQDPIIKASFEKFQGKLMELEGIIDL 472
PSQ+QAT ++AVLD+LS+HS DEEY+G +EP W+ +P+I A+FE F GKL ELEGIID
Sbjct: 241 PSQLQATKVIAVLDVLSNHSPDEEYIGTNIEPFWKDEPVINAAFEVFSGKLKELEGIIDA 300
Query: 471 SNADKSRKNRNGAGIVPYEL----AEASFRGRG 385
NAD + KNRNGAG++PYEL +E G+G
Sbjct: 301 RNADCNLKNRNGAGVMPYELLKPFSERGITGKG 333
Score = 38.5 bits (88), Expect = 0.12
Identities = 18/27 (66%), Positives = 20/27 (73%)
Frame = -1
Query: 431 GLCLMSLLKPVSEAGVTAKGVPYSISI 351
G+ LLKP SE G+T KGVPYSISI
Sbjct: 314 GVMPYELLKPFSERGITGKGVPYSISI 340
>pir||T07038 probable lipoxygenase (EC 1.13.11.12) Lox2 - tomato (fragment)
gi|2826844|emb|CAA05280.1| loxc homologue [Lycopersicon
esculentum]
Length = 442
Score = 228 bits (581), Expect = 8e-59
Identities = 104/153 (67%), Positives = 126/153 (81%), Gaps = 4/153 (2%)
Frame = -3
Query: 831 TIVWITSGHHAAVNFGQYSYAGYFPNRPTVARNNMPTEDPSDPEWELFLDKPEVTLLKCF 652
TI+W+TSGHHAAVNFGQYSYAGYFPNRPT+AR+ MPTEDP+D EWE FL+KPE LLKCF
Sbjct: 283 TIIWVTSGHHAAVNFGQYSYAGYFPNRPTIARSKMPTEDPTDEEWEDFLNKPEEALLKCF 342
Query: 651 PSQIQATTIMAVLDILSSHSSDEEYLGQTVEPSWEQDPIIKASFEKFQGKLMELEGIIDL 472
PSQ+QAT ++AVLD+LS+HS DEEY+G +EP W+ +P+I A+FE F GKL ELEGIID
Sbjct: 343 PSQLQATKVIAVLDVLSNHSPDEEYIGTNIEPFWKDEPVINAAFEVFSGKLKELEGIIDA 402
Query: 471 SNADKSRKNRNGAGIVPYEL----AEASFRGRG 385
NAD + KNRNGAG++PYEL +E G+G
Sbjct: 403 RNADCNLKNRNGAGVMPYELLKPFSEPGITGKG 435
Score = 38.5 bits (88), Expect = 0.12
Identities = 18/27 (66%), Positives = 20/27 (73%)
Frame = -1
Query: 431 GLCLMSLLKPVSEAGVTAKGVPYSISI 351
G+ LLKP SE G+T KGVPYSISI
Sbjct: 416 GVMPYELLKPFSEPGITGKGVPYSISI 442
>emb|CAA05270.1| loxc homologue [Lycopersicon hirsutum]
Length = 267
Score = 228 bits (581), Expect = 8e-59
Identities = 104/153 (67%), Positives = 126/153 (81%), Gaps = 4/153 (2%)
Frame = -3
Query: 831 TIVWITSGHHAAVNFGQYSYAGYFPNRPTVARNNMPTEDPSDPEWELFLDKPEVTLLKCF 652
TI+W+TSGHHAAVNFGQYSYAGYFPNRPT+AR+ MPTEDP+D EWE FL+KPE LLKCF
Sbjct: 108 TIIWVTSGHHAAVNFGQYSYAGYFPNRPTIARSKMPTEDPTDEEWEDFLNKPEEALLKCF 167
Query: 651 PSQIQATTIMAVLDILSSHSSDEEYLGQTVEPSWEQDPIIKASFEKFQGKLMELEGIIDL 472
PSQ+QAT ++AVLD+LS+HS DEEY+G +EP W+ +P+I A+FE F GKL ELEGIID
Sbjct: 168 PSQLQATKVIAVLDVLSNHSPDEEYIGTNIEPFWKDEPVINAAFEVFSGKLKELEGIIDA 227
Query: 471 SNADKSRKNRNGAGIVPYEL----AEASFRGRG 385
NAD + KNRNGAG++PYEL +E G+G
Sbjct: 228 RNADCNLKNRNGAGVMPYELLKPFSEPGITGKG 260
Score = 38.5 bits (88), Expect = 0.12
Identities = 18/27 (66%), Positives = 20/27 (73%)
Frame = -1
Query: 431 GLCLMSLLKPVSEAGVTAKGVPYSISI 351
G+ LLKP SE G+T KGVPYSISI
Sbjct: 241 GVMPYELLKPFSEPGITGKGVPYSISI 267
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 721,182,758
Number of Sequences: 1393205
Number of extensions: 15751992
Number of successful extensions: 38413
Number of sequences better than 10.0: 182
Number of HSP's better than 10.0 without gapping: 36522
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 38261
length of database: 448,689,247
effective HSP length: 122
effective length of database: 278,718,237
effective search space used: 43201326735
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
EST assemble image
|
|
|
|
|
|
clone |
accession |
position |
| 1 |
SPDL004c01_f |
BP052220 |
1 |
532 |
| 2 |
SPDL100d05_f |
BP058290 |
139 |
666 |
| 3 |
SPD057a08_f |
BP048503 |
228 |
590 |
| 4 |
MPDL017c08_f |
AV777344 |
228 |
778 |
| 5 |
MPDL054d12_f |
AV779244 |
228 |
756 |
| 6 |
MPDL082a02_f |
AV780742 |
231 |
797 |
| 7 |
SPDL077a11_f |
BP056754 |
238 |
734 |
| 8 |
MPDL078h08_f |
AV780559 |
239 |
705 |
| 9 |
SPD023f08_f |
BP045835 |
242 |
722 |
| 10 |
MFB085a06_f |
BP040185 |
243 |
835 |
| 11 |
SPDL007d09_f |
BP052412 |
245 |
755 |
| 12 |
SPDL038d10_f |
BP054391 |
247 |
771 |
| 13 |
SPDL017g02_f |
BP053083 |
250 |
755 |
|
Lotus japonicus
Kazusa DNA Research Institute