Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005499A_C01 KMC005499A_c01
(581 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_084798.1| RNA polymerase beta'' chain [Lotus japonicus] g... 388 e-107
gb|AAL07336.1| rpoC2 [Glycine max] 251 4e-66
sp|P12227|RPOD_PEA DNA-directed RNA polymerase beta" chain gi|81... 241 6e-63
ref|NP_783222.1| RNA polymerase beta II subunit [Atropa belladon... 229 1e-59
ref|NP_054486.1| RNA polymerase beta'' chain [Nicotiana tabacum]... 226 1e-58
>ref|NP_084798.1| RNA polymerase beta'' chain [Lotus japonicus]
gi|23822109|sp|Q9BBS7|RPOD_LOTJA DNA-directed RNA
polymerase beta" chain gi|13358979|dbj|BAB33196.1| RNA
polymerase beta' subunit-2 [Lotus japonicus]
Length = 1332
Score = 388 bits (997), Expect = e-107
Identities = 193/193 (100%), Positives = 193/193 (100%)
Frame = -2
Query: 580 SLLKDQDQMSTHSLPTAKRNTSNFLVSNNQVRLCPDHCHFMHPTISPDTSNLLAKKRRNR 401
SLLKDQDQMSTHSLPTAKRNTSNFLVSNNQVRLCPDHCHFMHPTISPDTSNLLAKKRRNR
Sbjct: 490 SLLKDQDQMSTHSLPTAKRNTSNFLVSNNQVRLCPDHCHFMHPTISPDTSNLLAKKRRNR 549
Query: 400 FIIPFLFRSIRERNNELMPDISVEIPIDGIIHKNSILAYFDDPQYRTQSSGIAKYKTIGI 221
FIIPFLFRSIRERNNELMPDISVEIPIDGIIHKNSILAYFDDPQYRTQSSGIAKYKTIGI
Sbjct: 550 FIIPFLFRSIRERNNELMPDISVEIPIDGIIHKNSILAYFDDPQYRTQSSGIAKYKTIGI 609
Query: 220 HSIFQKEDLIEYRGIREFKPKYQIKVDRFFFIPQEVHILSESSSIMVRNNSIIGVNTPIT 41
HSIFQKEDLIEYRGIREFKPKYQIKVDRFFFIPQEVHILSESSSIMVRNNSIIGVNTPIT
Sbjct: 610 HSIFQKEDLIEYRGIREFKPKYQIKVDRFFFIPQEVHILSESSSIMVRNNSIIGVNTPIT 669
Query: 40 LNKKSRVGGLVRV 2
LNKKSRVGGLVRV
Sbjct: 670 LNKKSRVGGLVRV 682
>gb|AAL07336.1| rpoC2 [Glycine max]
Length = 1384
Score = 251 bits (642), Expect = 4e-66
Identities = 136/221 (61%), Positives = 163/221 (73%), Gaps = 28/221 (12%)
Frame = -2
Query: 580 SLLKDQDQMSTHSLPTAKRNTSNFLVSNNQVR-------------------------LCP 476
S+ KDQDQM+ H L T +R+ N L S +VR +
Sbjct: 494 SIRKDQDQMNIHYLSTGERDFCNLLASKKKVRHKLFRFNPSDKKERRISDYSIFNQIISI 553
Query: 475 DHCHFMHPTISPDTSNLLAKKRRNRFIIPFLFRSIRERNNELM--PDISVEIPIDGIIHK 302
DHCHF HPTI DT++LLAK+RRNR IIPF F+SI+ER+ ELM IS+EIPI+GI +
Sbjct: 554 DHCHFTHPTIFHDTTDLLAKRRRNRLIIPFQFQSIQERDKELMLASSISIEIPINGIFRR 613
Query: 301 NSILAYFDDPQYRTQSSGIAKYKTIGIHSIFQKED-LIEYRGIREFKPKYQIKVDRFFFI 125
NSILAYFDDPQYRTQSSGI KY+TIGI+SIF+KED LIEY+G++EFK KYQIKVD+FFFI
Sbjct: 614 NSILAYFDDPQYRTQSSGITKYRTIGINSIFKKEDFLIEYQGVKEFKTKYQIKVDQFFFI 673
Query: 124 PQEVHILSESSSIMVRNNSIIGVNTPITLNKKSRVGGLVRV 2
P+EVHIL ESSSIMVRNNSI+ V+T ITLN +SRV GLVR+
Sbjct: 674 PEEVHILPESSSIMVRNNSIVEVDTLITLNIRSRVRGLVRL 714
>sp|P12227|RPOD_PEA DNA-directed RNA polymerase beta" chain gi|81957|pir||S07137
DNA-directed RNA polymerase (EC 2.7.7.6) beta'-2 chain -
garden pea chloroplast (fragment)
gi|829325|emb|CAA27545.1| ORF with large areas of
homology to RNA polymerase subunit beta [Pisum sativum]
Length = 1163
Score = 241 bits (614), Expect = 6e-63
Identities = 128/219 (58%), Positives = 153/219 (69%), Gaps = 27/219 (12%)
Frame = -2
Query: 577 LLKDQDQMSTHSLPTAKRNTSNFLVSNNQVR-------------------------LCPD 473
L KDQDQ++ SL K N SN L N+QV+ +C D
Sbjct: 287 LRKDQDQITMDSLSNGKTNISNLLERNDQVKHKLFRFNTFGTKEKGISDYSIFNEIICTD 346
Query: 472 HCHFMHPTISPDTSNLLAKKRRNRFIIPFLFRSIRERNNELMPD--ISVEIPIDGIIHKN 299
H + P I DT LAK+RRNRF+IPF F+SI+ER NE M +S+EIPI+GI H+N
Sbjct: 347 HSY---PAIFHDTFYFLAKRRRNRFLIPFPFQSIQERKNERMSPSGVSIEIPINGIFHRN 403
Query: 298 SILAYFDDPQYRTQSSGIAKYKTIGIHSIFQKEDLIEYRGIREFKPKYQIKVDRFFFIPQ 119
SI AYFDDPQYR SSGI KY+TIGIHSIFQKED IEYRGI+E KPK QI+VDRFFFIP+
Sbjct: 404 SIFAYFDDPQYRRHSSGITKYRTIGIHSIFQKEDFIEYRGIKELKPKSQIQVDRFFFIPE 463
Query: 118 EVHILSESSSIMVRNNSIIGVNTPITLNKKSRVGGLVRV 2
EVHIL +SSS+MVRNNS++G+ TPIT N +SRVGGLVR+
Sbjct: 464 EVHILPKSSSLMVRNNSLVGIGTPITFNIRSRVGGLVRL 502
>ref|NP_783222.1| RNA polymerase beta II subunit [Atropa belladonna]
gi|20068321|emb|CAC88034.1| RNA polymerase beta II
subunit [Atropa belladonna]
Length = 1389
Score = 229 bits (585), Expect = 1e-59
Identities = 124/220 (56%), Positives = 155/220 (70%), Gaps = 27/220 (12%)
Frame = -2
Query: 580 SLLKDQDQMSTHSLPTAKRNTSNFLVSNNQVR-------------------------LCP 476
S+ KDQDQM+ HSL +R TSN V+N+Q R +C
Sbjct: 494 SIHKDQDQMNAHSLSGKQRYTSNLSVTNDQARQKLFSSGFSGKKEDRIPDYSDLNRIICT 553
Query: 475 DHCHFMHPTISPDTSNLLAKKRRNRFIIPFLFRSIRERNNELMP--DISVEIPIDGIIHK 302
+ ++ I + S+LL+K+RRN+FIIP SI+E NELMP IS+EIP++GI +
Sbjct: 554 GQYNLVYSPILHENSDLLSKRRRNKFIIPL--HSIQELENELMPCSGISIEIPVNGIFRR 611
Query: 301 NSILAYFDDPQYRTQSSGIAKYKTIGIHSIFQKEDLIEYRGIREFKPKYQIKVDRFFFIP 122
NSILAYFDDP+YR +SSGI KY TI HS+ +KEDLIEYRG++EF+PKYQ+KVDRFFFIP
Sbjct: 612 NSILAYFDDPRYRRKSSGIIKYGTIETHSVIKKEDLIEYRGVKEFRPKYQMKVDRFFFIP 671
Query: 121 QEVHILSESSSIMVRNNSIIGVNTPITLNKKSRVGGLVRV 2
+EVHIL SSSIMVRNNSI+GV+T ITLN +SRVGGLVRV
Sbjct: 672 EEVHILPGSSSIMVRNNSIVGVDTQITLNLRSRVGGLVRV 711
>ref|NP_054486.1| RNA polymerase beta'' chain [Nicotiana tabacum]
gi|2924258|emb|CAA77410.1| RNA polymerase beta'' subunit
[Nicotiana tabacum]
Length = 1392
Score = 226 bits (577), Expect = 1e-58
Identities = 121/220 (55%), Positives = 154/220 (70%), Gaps = 27/220 (12%)
Frame = -2
Query: 580 SLLKDQDQMSTHSLPTAKRNTSNFLVSNNQVR-------------------------LCP 476
S+ KDQDQM+ H L +R TSN V+N+Q R +C
Sbjct: 494 SIHKDQDQMNAHFLSGKRRYTSNLSVTNDQARQKLFSSDFSGKKEDRIPDYSDLNRIICA 553
Query: 475 DHCHFMHPTISPDTSNLLAKKRRNRFIIPFLFRSIRERNNELMP--DISVEIPIDGIIHK 302
+ ++ I + S+LL+K+RRN+FIIP SI+E NELMP IS+EIP++GI +
Sbjct: 554 GQYNLVYSPILHENSDLLSKRRRNKFIIPL--HSIQELENELMPCSGISIEIPVNGIFRR 611
Query: 301 NSILAYFDDPQYRTQSSGIAKYKTIGIHSIFQKEDLIEYRGIREFKPKYQIKVDRFFFIP 122
NSILAYFDDP+YR +SSGI KY T+ HS+ +KEDL+EYRG++EF+PKYQ+KVDRFFFIP
Sbjct: 612 NSILAYFDDPRYRRKSSGIIKYGTVETHSVIKKEDLLEYRGVKEFRPKYQMKVDRFFFIP 671
Query: 121 QEVHILSESSSIMVRNNSIIGVNTPITLNKKSRVGGLVRV 2
+EVHIL SSSIMVRNNSI+GV+T ITLN +SRVGGLVRV
Sbjct: 672 EEVHILPGSSSIMVRNNSIVGVDTQITLNLRSRVGGLVRV 711
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 461,608,578
Number of Sequences: 1393205
Number of extensions: 9664736
Number of successful extensions: 22444
Number of sequences better than 10.0: 72
Number of HSP's better than 10.0 without gapping: 21726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22404
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21712003912
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
