>KMC005459A_C01 KMC005459A_c01
actggtgaaaaatagaaataattattAGAAGAAACTGAAAGATACATGAAGCCCATTACA
ACACCAGATGCTACATATTGCGTTAAAAAAAGACTTCATAGTTCTTACCAACCCATTAAG
GTTCTTCTAACTCTATCTGCAATTTGATATCTAGCAACAACACCTGTTAGTTGAAATTCA
CAATTCTCATCCAAAAGGAACCTGTAGACCTCCCATTCGCGCTCTGATGCTGAATGTCTT
CCTACTTCAGCCGAGAAAACACAGATTCCAAGTGCTTTGAGAGCAAATGTAGCATCATAA
AAAACTCGAGGTCTTCCCTTTCCAGATAGCTCAACTGGATTAGCGACTAATAGTTCAGTA
TCTGGTCCTCGGTTTGCAATGATGACTCGCAATGGATGAAGCATTTCCAGTTTCAAACGT
GAGCAGAGTGACCCTTGTTCTCAGGATCCAAAATCTTTTTCCCATCTTT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC005459A_C01 KMC005459A_c01
         (469 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAK74028.1| At2g39570/F12L6.23 [Arabidopsis thaliana] gi|2330...   171  4e-42
ref|NP_565908.1| expressed protein; protein id: At2g39570.1, sup...   171  4e-42
gb|AAM61017.1| unknown [Arabidopsis thaliana]                         125  3e-28
ref|NP_030235.1| expressed protein; protein id: At2g36840.1, sup...   125  3e-28
gb|AAO52165.1| similar to Dictyostelium discoideum (Slime mold)....    37  0.10

>gb|AAK74028.1| At2g39570/F12L6.23 [Arabidopsis thaliana]
           gi|23308377|gb|AAN18158.1| At2g39570/F12L6.23
           [Arabidopsis thaliana]
          Length = 411

 Score =  171 bits (432), Expect = 4e-42
 Identities = 81/110 (73%), Positives = 93/110 (83%)
 Frame = -3

Query: 437 QGSLCSRLKLEMLHPLRVIIANRGPDTELLVANPVELSGKGRPRVFYDATFALKALGICV 258
           Q + C+RLK EM+ PLRVII NRGPDTELLVANPVELSGKGRPRVFYD T ALK+LGIC+
Sbjct: 302 QANFCARLKEEMVCPLRVIIVNRGPDTELLVANPVELSGKGRPRVFYDVTLALKSLGICI 361

Query: 257 FSAEVGRHSASEREWEVYRFLLDENCEFQLTGVVARYQIADRVRRTLMGW 108
           FSAE+GRHS  +R+WEVYRFLLDE+ EF L  + AR Q+ DRV +TLMGW
Sbjct: 362 FSAEIGRHSTLDRQWEVYRFLLDESREFPLASLRARNQVVDRVTKTLMGW 411

>ref|NP_565908.1| expressed protein; protein id: At2g39570.1, supported by cDNA:
           gi_15010737 [Arabidopsis thaliana]
           gi|7485413|pir||T00567 hypothetical protein At2g39570
           [imported] - Arabidopsis thaliana
           gi|3355486|gb|AAC27848.1| expressed protein [Arabidopsis
           thaliana]
          Length = 411

 Score =  171 bits (432), Expect = 4e-42
 Identities = 81/110 (73%), Positives = 93/110 (83%)
 Frame = -3

Query: 437 QGSLCSRLKLEMLHPLRVIIANRGPDTELLVANPVELSGKGRPRVFYDATFALKALGICV 258
           Q + C+RLK EM+ PLRVII NRGPDTELLVANPVELSGKGRPRVFYD T ALK+LGIC+
Sbjct: 302 QANFCARLKEEMVCPLRVIIVNRGPDTELLVANPVELSGKGRPRVFYDVTLALKSLGICI 361

Query: 257 FSAEVGRHSASEREWEVYRFLLDENCEFQLTGVVARYQIADRVRRTLMGW 108
           FSAE+GRHS  +R+WEVYRFLLDE+ EF L  + AR Q+ DRV +TLMGW
Sbjct: 362 FSAEIGRHSTLDRQWEVYRFLLDESREFPLASLRARNQVVDRVTKTLMGW 411

>gb|AAM61017.1| unknown [Arabidopsis thaliana]
          Length = 410

 Score =  125 bits (313), Expect = 3e-28
 Identities = 59/108 (54%), Positives = 78/108 (71%)
 Frame = -3

Query: 431 SLCSRLKLEMLHPLRVIIANRGPDTELLVANPVELSGKGRPRVFYDATFALKALGICVFS 252
           +L +RL+ E+  PLRV++ NRGPDTELLV NPVELSGKGRP+VF+D   ALK +  C+FS
Sbjct: 306 ALITRLRAELQQPLRVVMMNRGPDTELLVTNPVELSGKGRPQVFHDIALALKKIDTCIFS 365

Query: 251 AEVGRHSASEREWEVYRFLLDENCEFQLTGVVARYQIADRVRRTLMGW 108
           AE+GRH   +REWEVY+ L++E         + R +I + V +TLMGW
Sbjct: 366 AEIGRHVTGDREWEVYKVLINEEDSLP----IPRSKIEEEVWKTLMGW 409

>ref|NP_030235.1| expressed protein; protein id: At2g36840.1, supported by cDNA:
           108427., supported by cDNA: gi_20260621 [Arabidopsis
           thaliana] gi|25341898|pir||D84785 hypothetical protein
           At2g36840 [imported] - Arabidopsis thaliana
           gi|4883601|gb|AAD31570.1| expressed protein [Arabidopsis
           thaliana] gi|20260622|gb|AAM13209.1| unknown protein
           [Arabidopsis thaliana] gi|23197994|gb|AAN15524.1|
           unknown protein [Arabidopsis thaliana]
          Length = 410

 Score =  125 bits (313), Expect = 3e-28
 Identities = 59/108 (54%), Positives = 78/108 (71%)
 Frame = -3

Query: 431 SLCSRLKLEMLHPLRVIIANRGPDTELLVANPVELSGKGRPRVFYDATFALKALGICVFS 252
           +L +RL+ E+  PLRV++ NRGPDTELLV NPVELSGKGRP+VF+D   ALK +  C+FS
Sbjct: 306 ALITRLRAELQQPLRVVMMNRGPDTELLVTNPVELSGKGRPQVFHDIALALKKIDTCIFS 365

Query: 251 AEVGRHSASEREWEVYRFLLDENCEFQLTGVVARYQIADRVRRTLMGW 108
           AE+GRH   +REWEVY+ L++E         + R +I + V +TLMGW
Sbjct: 366 AEIGRHVTGDREWEVYKVLINEEDSLP----IPRSKIEEEVWKTLMGW 409

>gb|AAO52165.1| similar to Dictyostelium discoideum (Slime mold). TipA
          Length = 768

 Score = 37.0 bits (84), Expect = 0.10
 Identities = 41/148 (27%), Positives = 62/148 (41%)
 Frame = +1

Query: 22  LLEETERYMKPITTPDATYCVKKRLHSSYQPIKVLLTLSAI*YLATTPVS*NSQFSSKRN 201
           LLEE +R    I      Y  K   +S+  PI +  +   +  L +TP + N + SS  +
Sbjct: 14  LLEENQRLKNEINELKQKYEPKSNRYSTSSPIAI--SSPNLSTLISTPTTLNGKISSTTS 71

Query: 202 L*TSHSRSDAECLPTSAEKTQIPSALRANVAS*KTRGLPFPDSSTGLATNSSVSGPRFAM 381
             TS S S     P+S+  T  P++    ++   T       SST +A  S+        
Sbjct: 72  TSTSPSPS-----PSSSSPTSSPTSSTTTISPSPT------SSSTSIALASTSPSSTSIA 120

Query: 382 MTRNG*SISSFKREQSDPCSQDPKSFSH 465
           +  +  +ISS     S P S  P S SH
Sbjct: 121 LASSPINISS---PPSSPISHQPISVSH 145

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 367,864,555
Number of Sequences: 1393205
Number of extensions: 6803857
Number of successful extensions: 13839
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 13601
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 13834
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 12232797444
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPDL032h01_f AV778100 1 405 2 SPDL014b02_f BP052842 27 469 3 MPDL005g03_f AV776787 67 226

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