Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005447A_C01 KMC005447A_c01
(712 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAA76834.1| SPINDLY protein [Petunia x hybrida] 148 8e-39
emb|CAC85168.1| SPY protein [Lycopersicon esculentum] gi|1991311... 144 9e-38
ref|NP_187761.1| spindly (gibberellin signal transduction protei... 128 1e-32
dbj|BAC11808.1| SPINDLY [Eustoma grandiflorum] 111 6e-26
gb|AAC36055.1| gibberellin action negative regulator SPY [Hordeu... 105 1e-24
>emb|CAA76834.1| SPINDLY protein [Petunia x hybrida]
Length = 932
Score = 148 bits (373), Expect(2) = 8e-39
Identities = 77/122 (63%), Positives = 94/122 (76%), Gaps = 2/122 (1%)
Frame = +1
Query: 298 MAWMEDS--DGKEKELAGENGFLKVSEASISSSGSAGDVDSAPNQGCEGKGDLSYANILR 471
MAW E +GKE + G NGFLK ++S S GS + S + EGK ++YANILR
Sbjct: 1 MAWTEKDVENGKESDSLGNNGFLKGVQSSSDSKGSPVRI-SPVKKSFEGKDAITYANILR 59
Query: 472 SRNKFVDSLAMYERVLETDNGNVEALIGKGICLQMQNMGRLAFDSLSEAIKLDPQNACAL 651
SRNKFVD+LA+YE VL+ D+G++E+LIGKGICLQMQNMGRLAF+S +EAIKLDPQNACAL
Sbjct: 60 SRNKFVDALAIYESVLQKDSGSIESLIGKGICLQMQNMGRLAFESFAEAIKLDPQNACAL 119
Query: 652 TH 657
TH
Sbjct: 120 TH 121
Score = 34.7 bits (78), Expect(2) = 8e-39
Identities = 15/17 (88%), Positives = 17/17 (99%)
Frame = +2
Query: 662 GILYKEEGRLMEAAESY 712
GILYK+EGRL+EAAESY
Sbjct: 123 GILYKDEGRLVEAAESY 139
>emb|CAC85168.1| SPY protein [Lycopersicon esculentum] gi|19913117|emb|CAC85169.1|
SPY protein [Lycopersicon esculentum]
Length = 931
Score = 144 bits (364), Expect(2) = 9e-38
Identities = 77/122 (63%), Positives = 92/122 (75%), Gaps = 2/122 (1%)
Frame = +1
Query: 298 MAWMEDS--DGKEKELAGENGFLKVSEASISSSGSAGDVDSAPNQGCEGKGDLSYANILR 471
MAW E +GKE E G NGFLK ++S S GS G + S + E K ++YANILR
Sbjct: 1 MAWTEKDVENGKESESLGNNGFLKGGQSSSGSKGSPGRI-SHVKKIFEDKDAITYANILR 59
Query: 472 SRNKFVDSLAMYERVLETDNGNVEALIGKGICLQMQNMGRLAFDSLSEAIKLDPQNACAL 651
SRNKFVD+LA+YE VLE D+ ++E+LIGKGICLQMQN GRLAF+S SEAIK+DPQNACAL
Sbjct: 60 SRNKFVDALAIYESVLEKDSKSIESLIGKGICLQMQNTGRLAFESFSEAIKVDPQNACAL 119
Query: 652 TH 657
TH
Sbjct: 120 TH 121
Score = 34.7 bits (78), Expect(2) = 9e-38
Identities = 15/17 (88%), Positives = 17/17 (99%)
Frame = +2
Query: 662 GILYKEEGRLMEAAESY 712
GILYK+EGRL+EAAESY
Sbjct: 123 GILYKDEGRLVEAAESY 139
>ref|NP_187761.1| spindly (gibberellin signal transduction protein); protein id:
At3g11540.1, supported by cDNA: gi_1589777 [Arabidopsis
thaliana] gi|1589778|gb|AAC49446.1| SPINDLY
gi|12322895|gb|AAG51433.1|AC008153_6 spindly
(gibberellin signal transduction protein); 75377-80082
[Arabidopsis thaliana]
Length = 914
Score = 128 bits (321), Expect(2) = 1e-32
Identities = 72/120 (60%), Positives = 83/120 (69%)
Frame = +1
Query: 298 MAWMEDSDGKEKELAGENGFLKVSEASISSSGSAGDVDSAPNQGCEGKGDLSYANILRSR 477
M +ED +E+ ENGF S S SSS SAG V S + +G LSYANILR+R
Sbjct: 1 MVGLEDDTERERSPVVENGF---SNGSRSSSSSAG-VLSPSRKVTQGNDTLSYANILRAR 56
Query: 478 NKFVDSLAMYERVLETDNGNVEALIGKGICLQMQNMGRLAFDSLSEAIKLDPQNACALTH 657
NKF D+LA+YE +LE D+ NVEA IGKGICLQ QN G LAFD SEAI+LDP NACALTH
Sbjct: 57 NKFADALALYEAMLEKDSKNVEAHIGKGICLQTQNKGNLAFDCFSEAIRLDPHNACALTH 116
Score = 33.9 bits (76), Expect(2) = 1e-32
Identities = 15/17 (88%), Positives = 17/17 (99%)
Frame = +2
Query: 662 GILYKEEGRLMEAAESY 712
GIL+KEEGRL+EAAESY
Sbjct: 118 GILHKEEGRLVEAAESY 134
>dbj|BAC11808.1| SPINDLY [Eustoma grandiflorum]
Length = 918
Score = 111 bits (277), Expect(2) = 6e-26
Identities = 63/107 (58%), Positives = 73/107 (67%)
Frame = +1
Query: 337 LAGENGFLKVSEASISSSGSAGDVDSAPNQGCEGKGDLSYANILRSRNKFVDSLAMYERV 516
+A G LK ++S S GS + EGK ++YA ILRSRNKFVD+LA+YE
Sbjct: 7 VAKPKGLLKGVQSSSDSKGSP------VKKSLEGKEAITYAKILRSRNKFVDALAIYE-- 58
Query: 517 LETDNGNVEALIGKGICLQMQNMGRLAFDSLSEAIKLDPQNACALTH 657
LE D+ NVEA IGKGICLQ QN G LAFD SEAI+LDP NACALTH
Sbjct: 59 LEKDSKNVEAHIGKGICLQTQNKGNLAFDCFSEAIRLDPHNACALTH 105
Score = 28.5 bits (62), Expect(2) = 6e-26
Identities = 12/14 (85%), Positives = 14/14 (99%)
Frame = +2
Query: 662 GILYKEEGRLMEAA 703
GILYK+EGRL+EAA
Sbjct: 107 GILYKDEGRLVEAA 120
>gb|AAC36055.1| gibberellin action negative regulator SPY [Hordeum vulgare]
Length = 944
Score = 105 bits (263), Expect(2) = 1e-24
Identities = 60/117 (51%), Positives = 71/117 (60%)
Frame = +1
Query: 307 MEDSDGKEKELAGENGFLKVSEASISSSGSAGDVDSAPNQGCEGKGDLSYANILRSRNKF 486
ME GKE NG + V + G A Q EG L YANILRSRNKF
Sbjct: 1 MESLQGKES-----NGAVPVC-----NGGGGAAAPPAKQQLPEGTDALRYANILRSRNKF 50
Query: 487 VDSLAMYERVLETDNGNVEALIGKGICLQMQNMGRLAFDSLSEAIKLDPQNACALTH 657
D+L +Y VL+ D NVEALIGKGICLQ Q++ R A D +EA+K+DP+NACALTH
Sbjct: 51 ADALQLYTTVLDKDGANVEALIGKGICLQAQSLPRQALDCFTEAVKVDPKNACALTH 107
Score = 29.6 bits (65), Expect(2) = 1e-24
Identities = 11/17 (64%), Positives = 16/17 (93%)
Frame = +2
Query: 662 GILYKEEGRLMEAAESY 712
G++YK+EG L+EAAE+Y
Sbjct: 109 GMIYKDEGHLVEAAEAY 125
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 695,361,074
Number of Sequences: 1393205
Number of extensions: 16719335
Number of successful extensions: 63412
Number of sequences better than 10.0: 92
Number of HSP's better than 10.0 without gapping: 58087
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 63196
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 32654539052
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
