Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005432A_C01 KMC005432A_c01
(613 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_084829.1| RNA polymerase alpha chain [Lotus japonicus] gi... 409 e-113
sp|P13911|RPOA_PEA DNA-directed RNA polymerase alpha chain (PEP)... 345 2e-94
gb|AAN04898.1| RNA polymerase alpha subunit [Vigna angularis] 343 1e-93
ref|NP_783263.1| RNA polymerase alpha subunit [Atropa belladonna... 322 2e-87
ref|NP_054532.1| RNA polymerase alpha chain [Nicotiana tabacum] ... 317 8e-86
>ref|NP_084829.1| RNA polymerase alpha chain [Lotus japonicus]
gi|22654080|sp|Q9BBQ4|RPOA_LOTJA DNA-directed RNA
polymerase alpha chain (PEP) (Plastid-encoded RNA
polymerase alpha subunit) (RNA polymerase alpha subunit)
gi|13359011|dbj|BAB33228.1| RNA polymerase alpha subunit
[Lotus japonicus]
Length = 333
Score = 409 bits (1052), Expect = e-113
Identities = 202/204 (99%), Positives = 203/204 (99%)
Frame = -2
Query: 612 TCITRAKFEKISHEYSTIVGIQEPVHEILMNLKEIVLKSNLYGIRDASICFKGPGYVTAQ 433
TCITRAK EKISHEYSTIVGIQEPVHEILMNLKEIVLKSNLYGIRDASICFKGPGYVTAQ
Sbjct: 60 TCITRAKSEKISHEYSTIVGIQEPVHEILMNLKEIVLKSNLYGIRDASICFKGPGYVTAQ 119
Query: 432 NIILPPSVVIVDNTQHIANVTEPIQLCIGLQIERDRGYRINTLKNFQDGSYNIDAIFMPV 253
NIILPPSVVIVDNTQHIANVTEPIQLCIGLQIERDRGYRINTLKNFQDGSYNIDAIFMPV
Sbjct: 120 NIILPPSVVIVDNTQHIANVTEPIQLCIGLQIERDRGYRINTLKNFQDGSYNIDAIFMPV 179
Query: 252 RNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALYEASRNLIDLFIPFLHAEEEKLNF 73
RNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALYEASRNLIDLFIPFLHAEEEKLNF
Sbjct: 180 RNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALYEASRNLIDLFIPFLHAEEEKLNF 239
Query: 72 ENHEHKVTLPLFTCHDLLAKTKLK 1
EN+EHKVTLPLFTCHDLLAKTKLK
Sbjct: 240 ENNEHKVTLPLFTCHDLLAKTKLK 263
>sp|P13911|RPOA_PEA DNA-directed RNA polymerase alpha chain (PEP) (Plastid-encoded RNA
polymerase alpha subunit) (RNA polymerase alpha subunit)
gi|81956|pir||S04384 DNA-directed RNA polymerase (EC
2.7.7.6) alpha chain - garden pea chloroplast
gi|12180|emb|CAA33668.1| RNA polymerase alpha subunit
(AA 1-334) [Pisum sativum]
Length = 334
Score = 345 bits (886), Expect = 2e-94
Identities = 169/196 (86%), Positives = 181/196 (92%)
Frame = -2
Query: 612 TCITRAKFEKISHEYSTIVGIQEPVHEILMNLKEIVLKSNLYGIRDASICFKGPGYVTAQ 433
TCITRAK EKI HEYSTIVGIQE +HEILMNLKEIVL+SNLYG R+ASICFKGPGYVTAQ
Sbjct: 60 TCITRAKSEKIPHEYSTIVGIQESIHEILMNLKEIVLRSNLYGTREASICFKGPGYVTAQ 119
Query: 432 NIILPPSVVIVDNTQHIANVTEPIQLCIGLQIERDRGYRINTLKNFQDGSYNIDAIFMPV 253
+IILP SV ++DNTQHIAN+TEPI LCI LQIER RGYRI TL N QDGSY IDA+FMPV
Sbjct: 120 DIILP-SVEVIDNTQHIANLTEPINLCIELQIERKRGYRIKTLNNIQDGSYTIDAVFMPV 178
Query: 252 RNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALYEASRNLIDLFIPFLHAEEEKLNF 73
RNANHSIHSYVNGN+KQEILFLEIWTNGSLTPKEALYEASRNLIDLFIPFLHAEEE LNF
Sbjct: 179 RNANHSIHSYVNGNQKQEILFLEIWTNGSLTPKEALYEASRNLIDLFIPFLHAEEENLNF 238
Query: 72 ENHEHKVTLPLFTCHD 25
EN++HK+TLPLFT HD
Sbjct: 239 ENNQHKMTLPLFTFHD 254
>gb|AAN04898.1| RNA polymerase alpha subunit [Vigna angularis]
Length = 333
Score = 343 bits (880), Expect = 1e-93
Identities = 167/204 (81%), Positives = 182/204 (88%)
Frame = -2
Query: 612 TCITRAKFEKISHEYSTIVGIQEPVHEILMNLKEIVLKSNLYGIRDASICFKGPGYVTAQ 433
TCITR K EKI HEYSTI+GI+E VHEI MNLKEIVLKSN+YG +DASI FKGPGY+TAQ
Sbjct: 60 TCITRVKSEKIPHEYSTIIGIEESVHEIFMNLKEIVLKSNMYGTQDASISFKGPGYITAQ 119
Query: 432 NIILPPSVVIVDNTQHIANVTEPIQLCIGLQIERDRGYRINTLKNFQDGSYNIDAIFMPV 253
+IILPPSV IVDN QHIANVTEP+ LCI L+IER+RGYRI TLKNFQDGSY+IDA FMPV
Sbjct: 120 DIILPPSVEIVDNRQHIANVTEPVNLCIELKIERNRGYRIKTLKNFQDGSYDIDARFMPV 179
Query: 252 RNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALYEASRNLIDLFIPFLHAEEEKLNF 73
RN N+SIHSYVNGNEKQEILFLEIWTNGSLTPKEALYEAS+NLIDLFIPFLHAEEE N
Sbjct: 180 RNVNYSIHSYVNGNEKQEILFLEIWTNGSLTPKEALYEASQNLIDLFIPFLHAEEENFNL 239
Query: 72 ENHEHKVTLPLFTCHDLLAKTKLK 1
E +HKVTLPLFT HD+L K KL+
Sbjct: 240 EKKKHKVTLPLFTFHDILVKDKLR 263
>ref|NP_783263.1| RNA polymerase alpha subunit [Atropa belladonna]
gi|20068363|emb|CAC88076.1| RNA polymerase alpha subunit
[Atropa belladonna]
Length = 337
Score = 322 bits (825), Expect = 2e-87
Identities = 157/200 (78%), Positives = 174/200 (86%)
Frame = -2
Query: 612 TCITRAKFEKISHEYSTIVGIQEPVHEILMNLKEIVLKSNLYGIRDASICFKGPGYVTAQ 433
TCITR KFEK+ HEYSTI GIQE VHEI+MNLKEIVL+SNLYG +AS+C KGPGYVTAQ
Sbjct: 60 TCITRVKFEKVPHEYSTITGIQESVHEIIMNLKEIVLRSNLYGTSEASVCVKGPGYVTAQ 119
Query: 432 NIILPPSVVIVDNTQHIANVTEPIQLCIGLQIERDRGYRINTLKNFQDGSYNIDAIFMPV 253
+IILPP V IVDNTQHIA++TEPI CIGLQIER+RGY I T +NFQDGSY IDA+FMPV
Sbjct: 120 DIILPPYVEIVDNTQHIASLTEPIDFCIGLQIERNRGYLIKTPQNFQDGSYPIDAVFMPV 179
Query: 252 RNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALYEASRNLIDLFIPFLHAEEEKLNF 73
RNANHSIHSY NGNEKQEILFLEIWTNGSLTPKEAL+EASRNLIDLFIPFLH EE+ L
Sbjct: 180 RNANHSIHSYGNGNEKQEILFLEIWTNGSLTPKEALHEASRNLIDLFIPFLHMEEDNLYL 239
Query: 72 ENHEHKVTLPLFTCHDLLAK 13
++++H V L FT HD LAK
Sbjct: 240 QDNQHTVPLSPFTFHDKLAK 259
>ref|NP_054532.1| RNA polymerase alpha chain [Nicotiana tabacum]
gi|133409|sp|P06269|RPOA_TOBAC DNA-directed RNA
polymerase alpha chain (PEP) (Plastid-encoded RNA
polymerase alpha subunit) (RNA polymerase alpha subunit)
gi|66962|pir||RNNTA DNA-directed RNA polymerase (EC
2.7.7.6) alpha chain - common tobacco chloroplast
gi|11860|emb|CAA77376.1| RNA polymerase alpha subunit
[Nicotiana tabacum] gi|10442805|gb|AAG15578.1| RNA
polymerase alpha subunit [synthetic construct]
gi|225228|prf||1211235BK RNA polymerase alpha
Length = 337
Score = 317 bits (812), Expect = 8e-86
Identities = 157/200 (78%), Positives = 171/200 (85%)
Frame = -2
Query: 612 TCITRAKFEKISHEYSTIVGIQEPVHEILMNLKEIVLKSNLYGIRDASICFKGPGYVTAQ 433
TCITR K EK+ HEYSTI GIQE VHEILMNLKEI+L+SNLYG DASIC KGPG VTAQ
Sbjct: 60 TCITRVKSEKVPHEYSTITGIQESVHEILMNLKEIILRSNLYGTSDASICVKGPGSVTAQ 119
Query: 432 NIILPPSVVIVDNTQHIANVTEPIQLCIGLQIERDRGYRINTLKNFQDGSYNIDAIFMPV 253
+IILPP V IVDNTQHIA++TEPI CIGLQIER+RGY I T NFQDGSY IDA+FMPV
Sbjct: 120 DIILPPYVEIVDNTQHIASLTEPIDFCIGLQIERNRGYLIKTPHNFQDGSYPIDAVFMPV 179
Query: 252 RNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALYEASRNLIDLFIPFLHAEEEKLNF 73
RNANHSIHSY NGNEKQEILFLEIWTNGSLTPKEAL+EASRNLIDLFIPFLH EE+ L
Sbjct: 180 RNANHSIHSYGNGNEKQEILFLEIWTNGSLTPKEALHEASRNLIDLFIPFLHMEEDNLYL 239
Query: 72 ENHEHKVTLPLFTCHDLLAK 13
++++H V L FT HD LAK
Sbjct: 240 QDNQHTVPLSPFTFHDKLAK 259
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 499,731,256
Number of Sequences: 1393205
Number of extensions: 10508445
Number of successful extensions: 26099
Number of sequences better than 10.0: 229
Number of HSP's better than 10.0 without gapping: 25097
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25885
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 24568846532
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
