Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005409A_C01 KMC005409A_c01
(541 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||T52138 eukaryotic cap-binding protein [imported] - Arabidop... 213 1e-54
ref|NP_197312.1| eukaryotic cap-binding protein (gb|AAC17220.1);... 210 1e-53
gb|AAC39871.1| translation initiation factor 4e [Homo sapiens] 139 3e-32
ref|NP_004837.1| eukaryotic translation initiation factor 4E-lik... 139 3e-32
gb|AAH49077.1| Similar to RIKEN cDNA 2700069E09 gene [Mus musculus] 137 8e-32
>pir||T52138 eukaryotic cap-binding protein [imported] - Arabidopsis thaliana
gi|3108209|gb|AAC17220.1| novel cap-binding protein nCBP
[Arabidopsis thaliana]
Length = 221
Score = 213 bits (542), Expect = 1e-54
Identities = 101/145 (69%), Positives = 118/145 (80%)
Frame = +2
Query: 107 NSEIADSPSQLAPDANNYCNEEAEERQARDLKAGLHPLKHKFVFWYTRRSPGVRTQTSYE 286
+ EI DS + + ++ + +EER++R+LK GLHPL++KF WYTRR+PGVR Q SYE
Sbjct: 8 DDEIRDSGNMDSIKSHYVTDSVSEERRSRELKDGLHPLRYKFSIWYTRRTPGVRNQ-SYE 66
Query: 287 DNIKKIVDFSTVEGFWVCYCHLARPSTLPSPTDLHLFKEGIRPLWEDSANGNGGKWIIRF 466
DNIKK+V+FSTVEGFW CYCHLAR S LPSPTDLH FK+GIRPLWED AN NGGKWIIRF
Sbjct: 67 DNIKKMVEFSTVEGFWACYCHLARSSLLPSPTDLHFFKDGIRPLWEDGANCNGGKWIIRF 126
Query: 467 KKVVSGRFWEDLVLALVGDQLDYGD 541
KVVS RFWEDL+LALVGDQLD D
Sbjct: 127 SKVVSARFWEDLLLALVGDQLDDAD 151
>ref|NP_197312.1| eukaryotic cap-binding protein (gb|AAC17220.1); protein id:
At5g18110.1, supported by cDNA: gi_20260129, supported
by cDNA: gi_20260617, supported by cDNA: gi_3108208
[Arabidopsis thaliana] gi|9758893|dbj|BAB09469.1|
eukaryotic cap-binding protein [Arabidopsis thaliana]
gi|20260130|gb|AAM12963.1| eukaryotic cap-binding
protein (gb|AAC17220.1) [Arabidopsis thaliana]
gi|20260618|gb|AAM13207.1| cap-binding protein
[Arabidopsis thaliana] gi|21386955|gb|AAM47881.1|
eukaryotic cap-binding protein [Arabidopsis thaliana]
Length = 221
Score = 210 bits (534), Expect = 1e-53
Identities = 100/145 (68%), Positives = 117/145 (79%)
Frame = +2
Query: 107 NSEIADSPSQLAPDANNYCNEEAEERQARDLKAGLHPLKHKFVFWYTRRSPGVRTQTSYE 286
+ EI DS + + ++ + +EER++R+LK G HPL++KF WYTRR+PGVR Q SYE
Sbjct: 8 DDEIRDSGNMDSIKSHYVTDSVSEERRSRELKDGDHPLRYKFSIWYTRRTPGVRNQ-SYE 66
Query: 287 DNIKKIVDFSTVEGFWVCYCHLARPSTLPSPTDLHLFKEGIRPLWEDSANGNGGKWIIRF 466
DNIKK+V+FSTVEGFW CYCHLAR S LPSPTDLH FK+GIRPLWED AN NGGKWIIRF
Sbjct: 67 DNIKKMVEFSTVEGFWACYCHLARSSLLPSPTDLHFFKDGIRPLWEDGANCNGGKWIIRF 126
Query: 467 KKVVSGRFWEDLVLALVGDQLDYGD 541
KVVS RFWEDL+LALVGDQLD D
Sbjct: 127 SKVVSARFWEDLLLALVGDQLDDAD 151
>gb|AAC39871.1| translation initiation factor 4e [Homo sapiens]
Length = 236
Score = 139 bits (349), Expect = 3e-32
Identities = 63/127 (49%), Positives = 89/127 (69%), Gaps = 1/127 (0%)
Frame = +2
Query: 164 NEEAEERQARDLKAGLHPLKHKFVFWYTRRSPGVRTQT-SYEDNIKKIVDFSTVEGFWVC 340
N+ + +R+A HPL++ + FWY+RR+PG T + SYE NIK+I F++VE FW
Sbjct: 29 NQSSSKRKAVVPGPAEHPLQYNYTFWYSRRTPGRPTSSQSYEQNIKQIGTFASVEQFWRF 88
Query: 341 YCHLARPSTLPSPTDLHLFKEGIRPLWEDSANGNGGKWIIRFKKVVSGRFWEDLVLALVG 520
Y H+ RP L +D HLFKEGI+P+WED AN NGGKWIIR +K ++ R WE+L+LA++G
Sbjct: 89 YSHMVRPGDLTGHSDFHLFKEGIKPMWEDDANKNGGKWIIRLRKGLASRCWENLILAMLG 148
Query: 521 DQLDYGD 541
+Q G+
Sbjct: 149 EQFMVGE 155
>ref|NP_004837.1| eukaryotic translation initiation factor 4E-like 3; eIF4E-like
cap-binding protein [Homo sapiens]
gi|3172339|gb|AAC18565.1| cap-binding protein 4EHP [Homo
sapiens] gi|3201960|gb|AAC19374.1| eIF4E-like protein
4E-LP [Homo sapiens] gi|13529272|gb|AAH05392.1|AAH05392
eukaryotic translation initiation factor 4E-like 3 [Homo
sapiens] gi|13543433|gb|AAH05874.1|AAH05874 eukaryotic
translation initiation factor 4E-like 3 [Homo sapiens]
gi|18203727|gb|AAH21226.1|AAH21226 eukaryotic
translation initiation factor 4E-like 3 [Homo sapiens]
gi|18203830|gb|AAH21690.1|AAH21690 eukaryotic
translation initiation factor 4E-like 3 [Homo sapiens]
Length = 245
Score = 139 bits (349), Expect = 3e-32
Identities = 63/127 (49%), Positives = 89/127 (69%), Gaps = 1/127 (0%)
Frame = +2
Query: 164 NEEAEERQARDLKAGLHPLKHKFVFWYTRRSPGVRTQT-SYEDNIKKIVDFSTVEGFWVC 340
N+ + +R+A HPL++ + FWY+RR+PG T + SYE NIK+I F++VE FW
Sbjct: 38 NQSSSKRKAVVPGPAEHPLQYNYTFWYSRRTPGRPTSSQSYEQNIKQIGTFASVEQFWRF 97
Query: 341 YCHLARPSTLPSPTDLHLFKEGIRPLWEDSANGNGGKWIIRFKKVVSGRFWEDLVLALVG 520
Y H+ RP L +D HLFKEGI+P+WED AN NGGKWIIR +K ++ R WE+L+LA++G
Sbjct: 98 YSHMVRPGDLTGHSDFHLFKEGIKPMWEDDANKNGGKWIIRLRKGLASRCWENLILAMLG 157
Query: 521 DQLDYGD 541
+Q G+
Sbjct: 158 EQFMVGE 164
>gb|AAH49077.1| Similar to RIKEN cDNA 2700069E09 gene [Mus musculus]
Length = 257
Score = 137 bits (345), Expect = 8e-32
Identities = 60/111 (54%), Positives = 82/111 (73%), Gaps = 1/111 (0%)
Frame = +2
Query: 212 HPLKHKFVFWYTRRSPGVRTQT-SYEDNIKKIVDFSTVEGFWVCYCHLARPSTLPSPTDL 388
HPL++ + FWY+RR+PG T + SYE NIK+I F++VE FW Y H+ RP L +D
Sbjct: 54 HPLQYNYTFWYSRRTPGRPTSSQSYEQNIKQIGTFASVEQFWKFYSHMVRPGDLTGHSDF 113
Query: 389 HLFKEGIRPLWEDSANGNGGKWIIRFKKVVSGRFWEDLVLALVGDQLDYGD 541
HLFKEGI+P+WED AN NGGKWIIR +K ++ R WE+L+LA++G+Q G+
Sbjct: 114 HLFKEGIKPMWEDDANKNGGKWIIRLRKGLASRCWENLILAMLGEQFMVGE 164
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 478,012,207
Number of Sequences: 1393205
Number of extensions: 10091882
Number of successful extensions: 28074
Number of sequences better than 10.0: 115
Number of HSP's better than 10.0 without gapping: 27227
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27996
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 18462123008
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
