Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005388A_C01 KMC005388A_c01
(569 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_568391.1| cytochrome-b5 reductase - like protein; protein... 186 2e-46
dbj|BAB62559.1| putative cytochrome b5 reductase [Oryza sativa (... 113 2e-32
gb|AAK69398.1|AF274589_1 cytochrome b5 reductase PP36 [Cucurbita... 134 6e-31
gb|AAD17694.1| cytochrome b5 reductase [Zea mays] 52 6e-10
gb|EAA35735.1| hypothetical protein [Neurospora crassa] 63 3e-09
>ref|NP_568391.1| cytochrome-b5 reductase - like protein; protein id: At5g20080.1,
supported by cDNA: 33544. [Arabidopsis thaliana]
gi|21592883|gb|AAM64833.1| cytochrome-b5 reductase-like
protein [Arabidopsis thaliana]
Length = 328
Score = 186 bits (471), Expect = 2e-46
Identities = 99/166 (59%), Positives = 120/166 (71%)
Frame = +3
Query: 51 FMRRVAKATPIAFRFSAGGGQSNNNNSSSFRLPFTAIAAISGAASLFYCSSSPYLVHSEQ 230
F RR+A++ PI F + G + + S +FR AIAA+SG S +Y +S LV+ +Q
Sbjct: 5 FFRRLARSAPITFPVAFGS--QSKSGSGAFRFSTGAIAALSGGFSYYYLTSGNNLVYLDQ 62
Query: 231 IAEEETKRDIALNPDKWLEFKLQDTARVSHNTQLFRFSFDPTKKLGLDIASCLLTRAPLG 410
A+EET ALNPDKWLEFKLQDTARVSHNTQLFRFSFDP+ +LGL +ASCLLTRAPLG
Sbjct: 63 -AKEETGPKTALNPDKWLEFKLQDTARVSHNTQLFRFSFDPSAELGLHVASCLLTRAPLG 121
Query: 411 QDAEGKPKYVVRPHTPISDPESKGYFDLLNQGVS*RKNEPTFCQLK 548
+AEGK KYV+RP+TPISDPE+KGYFDLL + K F LK
Sbjct: 122 YNAEGKTKYVIRPYTPISDPEAKGYFDLLIKVYPDGKMSQHFASLK 167
Score = 48.5 bits (114), Expect = 6e-05
Identities = 21/23 (91%), Positives = 23/23 (99%)
Frame = +1
Query: 499 IKVYPEGKMSQHFASLKPGDVVE 567
IKVYP+GKMSQHFASLKPGDV+E
Sbjct: 151 IKVYPDGKMSQHFASLKPGDVLE 173
>dbj|BAB62559.1| putative cytochrome b5 reductase [Oryza sativa (japonica
cultivar-group)]
Length = 294
Score = 113 bits (283), Expect(2) = 2e-32
Identities = 59/119 (49%), Positives = 77/119 (64%)
Frame = +3
Query: 141 RLPFTAIAAISGAASLFYCSSSPYLVHSEQIAEEETKRDIALNPDKWLEFKLQDTARVSH 320
R+P A AA++G A+LF SS P + E+ E+ + +ALNPDKWLEFKLQ+ A VSH
Sbjct: 16 RVPLAAAAAVTGGAALFCASSPPTIALMEEKGEDAAAK-VALNPDKWLEFKLQEKATVSH 74
Query: 321 NTQLFRFSFDPTKKLGLDIASCLLTRAPLGQDAEGKPKYVVRPHTPISDPESKGYFDLL 497
N+QLF RAP+G++ EG K+V+RP+TPISDP+SKGYFDLL
Sbjct: 75 NSQLF--------------------RAPIGEEVEGGRKFVIRPYTPISDPDSKGYFDLL 113
Score = 47.4 bits (111), Expect(2) = 2e-32
Identities = 21/28 (75%), Positives = 25/28 (89%)
Frame = +1
Query: 484 ILIC*IKVYPEGKMSQHFASLKPGDVVE 567
+L+ KVYP+GKMSQ+FASLKPGDVVE
Sbjct: 135 VLVVQCKVYPDGKMSQYFASLKPGDVVE 162
>gb|AAK69398.1|AF274589_1 cytochrome b5 reductase PP36 [Cucurbita maxima]
Length = 327
Score = 134 bits (338), Expect = 6e-31
Identities = 79/169 (46%), Positives = 104/169 (60%)
Frame = +3
Query: 42 MAFFMRRVAKATPIAFRFSAGGGQSNNNNSSSFRLPFTAIAAISGAASLFYCSSSPYLVH 221
MA F+RR+A A P A R++A GQS SS FR P I A++ S + +S+ LVH
Sbjct: 1 MAAFLRRLATAAP-ALRYNALCGQSRIE-SSKFRFPIGTITAVTAGFSYMFYASTSNLVH 58
Query: 222 SEQIAEEETKRDIALNPDKWLEFKLQDTARVSHNTQLFRFSFDPTKKLGLDIASCLLTRA 401
EE+ ++ +AL PDKW+EF+LQD ARVSHNT L+RFSFDP++ G + L
Sbjct: 59 LAPNCEEDGQK-VALKPDKWIEFELQDVARVSHNTNLYRFSFDPSENWGWMLLHASLQEL 117
Query: 402 PLGQDAEGKPKYVVRPHTPISDPESKGYFDLLNQGVS*RKNEPTFCQLK 548
L + G+ KYV R +TPISDPE+KGYFDLL + K F +LK
Sbjct: 118 QLIKTNRGEVKYVARSYTPISDPEAKGYFDLLIKIYPQGKMTQHFAKLK 166
Score = 50.4 bits (119), Expect = 2e-05
Identities = 32/79 (40%), Positives = 41/79 (51%), Gaps = 3/79 (3%)
Frame = +1
Query: 340 FRLIPLRSWVWILLLAFLPGLHWDKMQKENQNM---SYARIPLYRIQNPRDILIC*IKVY 510
F P +W W+LL A L L K + SY I + D+LI K+Y
Sbjct: 97 FSFDPSENWGWMLLHASLQELQLIKTNRGEVKYVARSYTPISDPEAKGYFDLLI---KIY 153
Query: 511 PEGKMSQHFASLKPGDVVE 567
P+GKM+QHFA LKPGD +E
Sbjct: 154 PQGKMTQHFAKLKPGDKLE 172
>gb|AAD17694.1| cytochrome b5 reductase [Zea mays]
Length = 279
Score = 52.4 bits (124), Expect(2) = 6e-10
Identities = 30/79 (37%), Positives = 47/79 (58%), Gaps = 1/79 (1%)
Frame = +3
Query: 264 LNPDKWLEFKLQDTARVSHNTQLFRFSF-DPTKKLGLDIASCLLTRAPLGQDAEGKPKYV 440
L+P+ + +FKL + ++SHN F+F+ PT LGL I + R GQDA G+ V
Sbjct: 40 LDPENFRKFKLVEKKQISHNVARFKFALPTPTSVLGLPIGQHISCR---GQDATGEE--V 94
Query: 441 VRPHTPISDPESKGYFDLL 497
++P+TP + GYF+L+
Sbjct: 95 IKPYTPTTLDSDLGYFELV 113
Score = 32.7 bits (73), Expect(2) = 6e-10
Identities = 12/20 (60%), Positives = 16/20 (80%)
Frame = +1
Query: 499 IKVYPEGKMSQHFASLKPGD 558
IK+YP+G+MS HF +K GD
Sbjct: 114 IKMYPQGRMSHHFREMKVGD 133
>gb|EAA35735.1| hypothetical protein [Neurospora crassa]
Length = 343
Score = 62.8 bits (151), Expect = 3e-09
Identities = 46/138 (33%), Positives = 70/138 (50%), Gaps = 9/138 (6%)
Frame = +3
Query: 111 QSNNNNSSSFRLPFTAIAAISGAASLFYCSS-------SPYLVHSEQIAEEETKRDIALN 269
+ + SSS L A ++GA + F+ + + +E+I E K+
Sbjct: 31 EPSKGGSSSTILLGAAAVGLAGAGAYFFSGAGAAKKAEASVKQVTEKITPGEIKKAFVGG 90
Query: 270 PDKWLEFKLQDTARVSHNTQLFRFSFDPTKKL-GLDIASCLLTR-APLGQDAEGKPKYVV 443
WL KL++ V+HNT+ RF + GL +AS +LT+ P+ DAE K V+
Sbjct: 91 DQGWLSLKLEEVELVNHNTKRLRFRLPEDDMVSGLHVASAILTKFKPI--DAE---KAVL 145
Query: 444 RPHTPISDPESKGYFDLL 497
RP+TPISD ++GY DLL
Sbjct: 146 RPYTPISDESAQGYIDLL 163
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 534,132,968
Number of Sequences: 1393205
Number of extensions: 12822255
Number of successful extensions: 70011
Number of sequences better than 10.0: 362
Number of HSP's better than 10.0 without gapping: 53701
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 66919
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20956655091
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
