>KMC005277A_C01 KMC005277A_c01
tacgggccccctcgagttTTTTTTTTTTTTTTTTGACACACCAATTCAAATCCATTAACA
GCAATAGATCCTTCATACACCCTATGTAACAATATGATGGAACCATAGGTAGCCCATGGA
TCATAGCTGTAGGTGGTGGGCGTTTTATTCAAATTATGATACAGAAACCAGAAAAGGAGC
AGTTGGCCTTGGCTAACAAAACTCCCAGGAAAGCCACACAGAACACCAGAGACCATATCG
CTGTTAATCATTAATCACACAAAGCCACAATTAGTTATTCAGATTGGAAATGCCTCAATG
ATGGATGGCAGGGCATTGATCTTGATGATGTTGTAACGAGGGAATCCTGTTTCTTTAAAT
AGATATTGCCAGTTCTCTTCAGTCCTTTCTTTCCCACCAGAGTTGTGTGCGAGCAGCATC
ATGTCAAATGCATGGCCAGTGTCAGAAAATGGTTCATTCCCTTCAGGCTGCAAAACATGA
TCCACAATGATGACCTTTCCAGTCTTCTCTGGTATTGCCTCTCTGCAGTTCTTCAAAATC
TTGATGCAATGCTCATCGCTCCAGTCATGAAGAATCCACTTCATGTAAATAGCATCAGCA
TTAGGAACAGAG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC005277A_C01 KMC005277A_c01
         (612 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAO33590.1|AF479308_1 putative caffeic acid methyl transferas...   201  8e-51
gb|AAC49708.1| caffeic acid O-methyltransferase                       136  2e-31
gb|AAD24001.1|AF119225_1 caffeic acid ortho-methyltransferase [P...   133  2e-30
pir||T09600 catechol O-methyltransferase homolog - Monterey pine...   133  2e-30
sp|Q9LEL6|6OMT_COPJA (R,S)-norcoclaurine 6-O-methyltransferase (...   114  1e-24

>gb|AAO33590.1|AF479308_1 putative caffeic acid methyl transferase [Arachis hypogaea]
          Length = 121

 Score =  201 bits (510), Expect = 8e-51
 Identities = 95/109 (87%), Positives = 101/109 (92%)
 Frame = -2

Query: 611 SVPNADAIYMKWILHDWSDEHCIKILKNCREAIPEKTGKVIIVDHVLQPEGNEPFSDTGH 432
           SVP ADAIYMKWILHDWSD+HCIKILKNCR+AIPE  GKVIIVDHVLQPEGN+ F DTG 
Sbjct: 13  SVPKADAIYMKWILHDWSDQHCIKILKNCRKAIPEN-GKVIIVDHVLQPEGNDLFDDTGF 71

Query: 431 AFDMMLLAHNSGGKERTEENWQYLFKETGFPRYNIIKINALPSIIEAFP 285
           AFDMMLLAHN+GGKERTEENW++LF ETGFPRYNIIKI ALPSIIEAFP
Sbjct: 72  AFDMMLLAHNAGGKERTEENWKWLFAETGFPRYNIIKIKALPSIIEAFP 120

>gb|AAC49708.1| caffeic acid O-methyltransferase
          Length = 381

 Score =  136 bits (342), Expect = 2e-31
 Identities = 68/118 (57%), Positives = 86/118 (72%), Gaps = 9/118 (7%)
 Frame = -2

Query: 611 SVPNADAIYMKWILHDWSDEHCIKILKNCREAIPEKTGKVIIVDHVLQPEGNEPFSD--- 441
           +VP ADAI+MKWI+HDW+DE CIKILKNCR+AIP+ TGKVIIVD VL  +  +       
Sbjct: 264 TVPTADAIFMKWIMHDWNDEDCIKILKNCRKAIPD-TGKVIIVDVVLDADQGDNTDKKRK 322

Query: 440 ------TGHAFDMMLLAHNSGGKERTEENWQYLFKETGFPRYNIIKINALPSIIEAFP 285
                  G  FD++++AH+SGGKERTE+ W+ +  E GF RYNII+I AL S+IEAFP
Sbjct: 323 KAVDPIVGTVFDLVMVAHSSGGKERTEKEWKRILLEGGFSRYNIIEIPALQSVIEAFP 380

>gb|AAD24001.1|AF119225_1 caffeic acid ortho-methyltransferase [Pinus radiata]
          Length = 382

 Score =  133 bits (334), Expect = 2e-30
 Identities = 66/118 (55%), Positives = 85/118 (71%), Gaps = 9/118 (7%)
 Frame = -2

Query: 611 SVPNADAIYMKWILHDWSDEHCIKILKNCREAIPEKTGKVIIVDHVLQPEGNEPFSD--- 441
           +VP  DAI+MKWI+HDW+DE CIKILKNCR+AIP+ TGKVIIVD VL  +  +       
Sbjct: 265 TVPTGDAIFMKWIMHDWNDEDCIKILKNCRKAIPD-TGKVIIVDVVLDADQGDNTDKKRK 323

Query: 440 ------TGHAFDMMLLAHNSGGKERTEENWQYLFKETGFPRYNIIKINALPSIIEAFP 285
                  G  FD++++AH+SGGKER+E+ W+ +  E GF RYNII+I AL S+IEAFP
Sbjct: 324 KAVDPIVGTVFDLVMVAHSSGGKERSEKEWKRILLEGGFSRYNIIEIPALQSVIEAFP 381

>pir||T09600 catechol O-methyltransferase homolog - Monterey pine
           gi|1568664|gb|AAB09044.1| O-methyltransferase [Pinus
           radiata]
          Length = 382

 Score =  133 bits (334), Expect = 2e-30
 Identities = 66/118 (55%), Positives = 85/118 (71%), Gaps = 9/118 (7%)
 Frame = -2

Query: 611 SVPNADAIYMKWILHDWSDEHCIKILKNCREAIPEKTGKVIIVDHVLQPEGNEPFSD--- 441
           +VP  DAI+MKWI+HDW+DE CIKILKNCR+AIP+ TGKVIIVD VL  +  +       
Sbjct: 265 TVPTGDAIFMKWIMHDWNDEDCIKILKNCRKAIPD-TGKVIIVDVVLDADQGDNTDKKRK 323

Query: 440 ------TGHAFDMMLLAHNSGGKERTEENWQYLFKETGFPRYNIIKINALPSIIEAFP 285
                  G  FD++++AH+SGGKER+E+ W+ +  E GF RYNII+I AL S+IEAFP
Sbjct: 324 KAVDPIVGTVFDLVMVAHSSGGKERSEKEWKRILLEGGFSRYNIIEIPALQSVIEAFP 381

>sp|Q9LEL6|6OMT_COPJA (R,S)-norcoclaurine 6-O-methyltransferase
           (S-adenosyl-L-methionine:norcoclaurine
           6-O-methyltransferase) (6-OMT)
           gi|9711914|dbj|BAB08004.1|
           S-adenosyl-L-methionine:norcoclaurine
           6-O-methyltransferase [Coptis japonica]
          Length = 347

 Score =  114 bits (285), Expect = 1e-24
 Identities = 54/108 (50%), Positives = 74/108 (68%)
 Frame = -2

Query: 608 VPNADAIYMKWILHDWSDEHCIKILKNCREAIPEKTGKVIIVDHVLQPEGNEPFSDTGHA 429
           +P ADAI MK ILHDW D+ CI+ILK C+EA+P K GKVIIVD VL  +   P++     
Sbjct: 240 IPKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLT 299

Query: 428 FDMMLLAHNSGGKERTEENWQYLFKETGFPRYNIIKINALPSIIEAFP 285
            D+ ++  N+GGKERTEE W+ L  + G+  + I +I A+ S+IEA+P
Sbjct: 300 LDLDMML-NTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYP 346

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 563,458,223
Number of Sequences: 1393205
Number of extensions: 12372661
Number of successful extensions: 31657
Number of sequences better than 10.0: 200
Number of HSP's better than 10.0 without gapping: 30337
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 31477
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 24568846532
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 SPDL077c02_f BP056765 1 282 2 SPD022b06_f BP045706 6 584 3 SPD060f11_f BP048790 19 524 4 MPD045e02_f AV773056 33 300 5 SPDL003a09_f BP052150 34 414 6 MFB003d09_f BP034112 34 479 7 MFB051c11_f BP037692 35 603 8 MWM143d11_f AV766963 35 455 9 MFB019c06_f BP035327 35 526 10 MFB039e06_f BP036863 35 625 11 MFB047f09_f BP037437 38 333 12 MFB006c05_f BP034322 38 508

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