Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005271A_C01 KMC005271A_c01
(1045 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAB75428.1| enolase [Lupinus luteus] 569 e-161
sp|P42896|ENO_RICCO Enolase (2-phosphoglycerate dehydratase) (2-... 568 e-161
sp|Q42971|ENO_ORYSA Enolase (2-phosphoglycerate dehydratase) (2-... 565 e-160
sp|Q9LEI9|ENO2_HEVBR Enolase 2 (2-phosphoglycerate dehydratase 2... 563 e-159
ref|NP_181192.1| enolase (2-phospho-D-glycerate hydroylase); pro... 563 e-159
>emb|CAB75428.1| enolase [Lupinus luteus]
Length = 444
Score = 569 bits (1467), Expect = e-161
Identities = 283/323 (87%), Positives = 303/323 (93%)
Frame = +3
Query: 75 MATITTIKARQIFDSRGNPTVEVDITCSDGTFARAAVPSGASTGIYEALE*RDGGSDYLG 254
MATI TIKARQIFDSRGNPTVEVD+T SDGTFARAAVPSGASTGIYEALE RDGGSDYLG
Sbjct: 1 MATIATIKARQIFDSRGNPTVEVDLTLSDGTFARAAVPSGASTGIYEALELRDGGSDYLG 60
Query: 255 KGVSKAVNNVNTIIAPAVIGQDPTQQTAIDNLMVQQRDGTVNEWGRCKQKLGANAILAVS 434
KGVSKAV+NVNTIIAPA+IG+DPT+QTAIDN +VQ+ DGTVNEWG KQKLGANAILAVS
Sbjct: 61 KGVSKAVDNVNTIIAPALIGKDPTEQTAIDNFIVQELDGTVNEWGWGKQKLGANAILAVS 120
Query: 435 LAVCKAGALVLKVPLYKHIANIAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMVLPVGA 614
LAVCKAGA LK+PLYKHIAN+AGNKNLVLPVPAFNVINGGSHAGNKLAMQEFM+LPVGA
Sbjct: 121 LAVCKAGASALKIPLYKHIANLAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
Query: 615 SSFKEAMKMGVEVYHHLKAVIKKKYGQDAVNVGDEGGFAPNIQENKEGLELLKTAIAKAG 794
S+FKEA+K+GVEVYH+LK+VIKKKYGQDAVNVGDEGGFAPNIQENKEGLELLK+AIAKAG
Sbjct: 181 STFKEALKIGVEVYHNLKSVIKKKYGQDAVNVGDEGGFAPNIQENKEGLELLKSAIAKAG 240
Query: 795 YTGKVVIGMDVAASEFYKEDKTYDLNFKEDNNDGSQKISGDALKDLYKSFVTEYPIVSIE 974
YT KVVIGMDVAASEFY DK Y LNFKEDNNDGSQKISG+ LK+LYKSFV EYPI SIE
Sbjct: 241 YTDKVVIGMDVAASEFYTSDKKYVLNFKEDNNDGSQKISGEELKELYKSFVAEYPIASIE 300
Query: 975 DPFDQDDWEHYSKLTGEVGTNVQ 1043
DPFDQDDWEHY K+T E+GT VQ
Sbjct: 301 DPFDQDDWEHYGKITTEIGTEVQ 323
>sp|P42896|ENO_RICCO Enolase (2-phosphoglycerate dehydratase) (2-phospho-D-glycerate
hydro-lyase) gi|542019|pir||S39203 phosphopyruvate
hydratase (EC 4.2.1.11) - castor bean
gi|433609|emb|CAA82232.1| enolase [Ricinus communis]
Length = 445
Score = 568 bits (1463), Expect = e-161
Identities = 280/321 (87%), Positives = 298/321 (92%)
Frame = +3
Query: 81 TITTIKARQIFDSRGNPTVEVDITCSDGTFARAAVPSGASTGIYEALE*RDGGSDYLGKG 260
TI +++ARQIFDSRGNPTVE DI SDG ARAAVPSGASTGIYEALE RDGGSDYLGKG
Sbjct: 4 TIVSVRARQIFDSRGNPTVEADIKLSDGHLARAAVPSGASTGIYEALELRDGGSDYLGKG 63
Query: 261 VSKAVNNVNTIIAPAVIGQDPTQQTAIDNLMVQQRDGTVNEWGRCKQKLGANAILAVSLA 440
VSKAV NVN+II PA+IG+DPT+QTA+DN MVQ+ DGTVNEWG CKQKLGANAILAVSLA
Sbjct: 64 VSKAVENVNSIIGPALIGKDPTEQTALDNFMVQELDGTVNEWGWCKQKLGANAILAVSLA 123
Query: 441 VCKAGALVLKVPLYKHIANIAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMVLPVGASS 620
+CKAGA V +PLYKHIAN+AGNKNLVLPVPAFNVINGGSHAGNKLAMQEFM+LPVGASS
Sbjct: 124 LCKAGAHVKGIPLYKHIANLAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS 183
Query: 621 FKEAMKMGVEVYHHLKAVIKKKYGQDAVNVGDEGGFAPNIQENKEGLELLKTAIAKAGYT 800
FKEAMKMG EVYHHLK+VIKKKYGQDA NVGDEGGFAPNIQENKEGLELLKTAIAKAGYT
Sbjct: 184 FKEAMKMGAEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAGYT 243
Query: 801 GKVVIGMDVAASEFYKEDKTYDLNFKEDNNDGSQKISGDALKDLYKSFVTEYPIVSIEDP 980
GKVVIGMDVAASEFY DKTYDLNFKE+NNDGSQKISG+ALKDLYKSF +EYPIVSIEDP
Sbjct: 244 GKVVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGEALKDLYKSFASEYPIVSIEDP 303
Query: 981 FDQDDWEHYSKLTGEVGTNVQ 1043
FDQDDWEHYSKLT E+G VQ
Sbjct: 304 FDQDDWEHYSKLTSEIGEKVQ 324
>sp|Q42971|ENO_ORYSA Enolase (2-phosphoglycerate dehydratase) (2-phospho-D-glycerate
hydro-lyase) (OSE1) gi|7436887|pir||T03267 probable
phosphopyruvate hydratase (EC 4.2.1.11) - rice
gi|780372|gb|AAC49173.1| enolase
Length = 446
Score = 565 bits (1456), Expect = e-160
Identities = 274/323 (84%), Positives = 303/323 (92%), Gaps = 1/323 (0%)
Frame = +3
Query: 78 ATITTIKARQIFDSRGNPTVEVDITCSDGTFARAAVPSGASTGIYEALE*RDGGSDYLGK 257
ATI ++KARQIFDSRGNPTVEVD+ CSDGTFARAAVPSGASTG+YEALE RDGGSDYLGK
Sbjct: 3 ATIVSVKARQIFDSRGNPTVEVDVCCSDGTFARAAVPSGASTGVYEALELRDGGSDYLGK 62
Query: 258 GVSKAVNNVNTIIAPAVIGQDPTQQTAIDNLMVQQRDGTVNEWGRCKQKLGANAILAVSL 437
GVSKAV+NVN++IAPA+IG+DPT Q +DN MVQQ DGT NEWG CKQKLGANAILAVSL
Sbjct: 63 GVSKAVDNVNSVIAPALIGKDPTSQAELDNFMVQQLDGTKNEWGWCKQKLGANAILAVSL 122
Query: 438 AVCKAGALVLKVPLYKHIANIAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMVLPVGAS 617
A+CKAGA++ K+PLY+HIAN+AGNK LVLPVPAFNVINGGSHAGNKLAMQEFM+LP GA+
Sbjct: 123 AICKAGAIIKKIPLYQHIANLAGNKQLVLPVPAFNVINGGSHAGNKLAMQEFMILPTGAA 182
Query: 618 SFKEAMKMGVEVYHHLKAVIKKKYGQDAVNVGDEGGFAPNIQENKEGLELLKTAIAKAGY 797
SFKEAMKMGVEVYH+LK+VIKKKYGQDA NVGDEGGFAPNIQENKEGLELLKTAI KAGY
Sbjct: 183 SFKEAMKMGVEVYHNLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGY 242
Query: 798 TGKVVIGMDVAASEFYKE-DKTYDLNFKEDNNDGSQKISGDALKDLYKSFVTEYPIVSIE 974
TGKVVIGMDVAASEFY + DKTYDLNFKE+NNDGSQKISGD+LK++YKSFV+EYPIVSIE
Sbjct: 243 TGKVVIGMDVAASEFYNDKDKTYDLNFKEENNDGSQKISGDSLKNVYKSFVSEYPIVSIE 302
Query: 975 DPFDQDDWEHYSKLTGEVGTNVQ 1043
DPFDQDDWEHY+K+T E+G VQ
Sbjct: 303 DPFDQDDWEHYAKMTAEIGEQVQ 325
>sp|Q9LEI9|ENO2_HEVBR Enolase 2 (2-phosphoglycerate dehydratase 2) (2-phospho-D-glycerate
hydro-lyase 2) (Allergen Hev b 9)
gi|9581746|emb|CAC00533.1| enolase, isoform 2 [Hevea
brasiliensis]
Length = 445
Score = 563 bits (1451), Expect = e-159
Identities = 277/321 (86%), Positives = 293/321 (90%)
Frame = +3
Query: 81 TITTIKARQIFDSRGNPTVEVDITCSDGTFARAAVPSGASTGIYEALE*RDGGSDYLGKG 260
TI +++ARQIFDSRGNPTVE D+ SDG ARAAVP GASTGIYEALE RDGGSDYLGKG
Sbjct: 4 TIVSVRARQIFDSRGNPTVEADVKLSDGYLARAAVPRGASTGIYEALELRDGGSDYLGKG 63
Query: 261 VSKAVNNVNTIIAPAVIGQDPTQQTAIDNLMVQQRDGTVNEWGRCKQKLGANAILAVSLA 440
VSKAV NVN II PA++G+DPT Q IDN MVQQ DGTVNEWG CKQKLGANAILAVSLA
Sbjct: 64 VSKAVENVNIIIGPALVGKDPTDQVGIDNFMVQQLDGTVNEWGWCKQKLGANAILAVSLA 123
Query: 441 VCKAGALVLKVPLYKHIANIAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMVLPVGASS 620
VCKAGA V +PLYKH+AN+AGNKNLVLPVPAFNVINGGSHAGNKLAMQEFM+LPVGASS
Sbjct: 124 VCKAGAHVKGIPLYKHVANLAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASS 183
Query: 621 FKEAMKMGVEVYHHLKAVIKKKYGQDAVNVGDEGGFAPNIQENKEGLELLKTAIAKAGYT 800
FKEAMKMG EVYHHLK+VIKKKYGQDA NVGDEGGFAPNIQENKEGLELLKTAIAKAGYT
Sbjct: 184 FKEAMKMGAEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIAKAGYT 243
Query: 801 GKVVIGMDVAASEFYKEDKTYDLNFKEDNNDGSQKISGDALKDLYKSFVTEYPIVSIEDP 980
GKVVIGMDVAASEFY DKTYDLNFKE+NN+GSQKISGD LKDLYKSFVTEYPIVSIEDP
Sbjct: 244 GKVVIGMDVAASEFYGSDKTYDLNFKEENNNGSQKISGDVLKDLYKSFVTEYPIVSIEDP 303
Query: 981 FDQDDWEHYSKLTGEVGTNVQ 1043
FDQDDWEHY+KLT E+G VQ
Sbjct: 304 FDQDDWEHYAKLTSEIGVKVQ 324
>ref|NP_181192.1| enolase (2-phospho-D-glycerate hydroylase); protein id: At2g36530.1,
supported by cDNA: gi_15809969, supported by cDNA:
gi_15983457, supported by cDNA: gi_16226234, supported by
cDNA: gi_18175727, supported by cDNA: gi_20260173
[Arabidopsis thaliana] gi|119350|sp|P25696|ENO_ARATH
Enolase (2-phosphoglycerate dehydratase)
(2-phospho-D-glycerate hydro-lyase) gi|81608|pir||JQ1187
phosphopyruvate hydratase (EC 4.2.1.11) - Arabidopsis
thaliana gi|16271|emb|CAA41114.1| enolase [Arabidopsis
thaliana] gi|4581151|gb|AAD24635.1| enolase
(2-phospho-D-glycerate hydroylase) [Arabidopsis thaliana]
gi|15983458|gb|AAL11597.1|AF424603_1 At2g36530/F1O11.16
[Arabidopsis thaliana] gi|23297411|gb|AAN12963.1| enolase
(2-phospho-D-glycerate hydroylase) [Arabidopsis thaliana]
Length = 444
Score = 563 bits (1451), Expect = e-159
Identities = 280/323 (86%), Positives = 296/323 (90%)
Frame = +3
Query: 75 MATITTIKARQIFDSRGNPTVEVDITCSDGTFARAAVPSGASTGIYEALE*RDGGSDYLG 254
MATIT +KARQIFDSRGNPTVEVDI S+G AAVPSGASTGIYEALE RDGGSDYLG
Sbjct: 1 MATITVVKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDGGSDYLG 60
Query: 255 KGVSKAVNNVNTIIAPAVIGQDPTQQTAIDNLMVQQRDGTVNEWGRCKQKLGANAILAVS 434
KGVSKAV NVN II PA+IG+DPTQQTAIDN MV + DGT NEWG CKQKLGANAILAVS
Sbjct: 61 KGVSKAVGNVNNIIGPALIGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVS 120
Query: 435 LAVCKAGALVLKVPLYKHIANIAGNKNLVLPVPAFNVINGGSHAGNKLAMQEFMVLPVGA 614
LAVCKAGA+V +PLYKHIAN+AGN +VLPVPAFNVINGGSHAGNKLAMQEFM+LPVGA
Sbjct: 121 LAVCKAGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 180
Query: 615 SSFKEAMKMGVEVYHHLKAVIKKKYGQDAVNVGDEGGFAPNIQENKEGLELLKTAIAKAG 794
+SFKEAMKMGVEVYHHLK+VIKKKYGQDA NVGDEGGFAPNIQENKEGLELLKTAI KAG
Sbjct: 181 ASFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAG 240
Query: 795 YTGKVVIGMDVAASEFYKEDKTYDLNFKEDNNDGSQKISGDALKDLYKSFVTEYPIVSIE 974
YTGKVVIGMDVAASEFY EDKTYDLNFKE+NN+GSQKISGDALKDLYKSFV EYPIVSIE
Sbjct: 241 YTGKVVIGMDVAASEFYSEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSIE 300
Query: 975 DPFDQDDWEHYSKLTGEVGTNVQ 1043
DPFDQDDWEHY+K+T E GT VQ
Sbjct: 301 DPFDQDDWEHYAKMTTECGTEVQ 323
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 972,575,608
Number of Sequences: 1393205
Number of extensions: 22873090
Number of successful extensions: 71322
Number of sequences better than 10.0: 573
Number of HSP's better than 10.0 without gapping: 64141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 69432
length of database: 448,689,247
effective HSP length: 124
effective length of database: 275,931,827
effective search space used: 61532797421
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
