>KMC005239A_C01 KMC005239A_c01
gttgttggaaacaaacaaatcttactcattaaacacaaactggaagagcttaccatcagt
aatacacagaagatgagtcgGTTTCCTCCTAGAACGTGCATTGGCCAGGGAATATATCTG
AGAAAGCATGAGGGTTAACAGTAACAACATCTACAGGGCACTCTTTCTCATTTAGGCTGG
TCTGGTCTCTTACCATGTTGGTGCTGGTTTCACATCCTCCCTACCTGAATCACAAGGAAG
AGAAAATCCTGGGCTGTTCTTTCTGAGATAGATTACCTGTATATTGGGGGCTGTCCAAAT
TACTATCAGAAAGGTGAAACGGGTAAGTCTAGAGAGCAGTTAAGTACATGATAGATTTTC
TCCTGCCATGAAAATTGGAGCTGACACACAGCAATACAACATAGATTCACAATCAGACAA
AGCTTACAAGCAACAACTAGAAAACAGAAAGAAAGAGAGAGAATGGGAAAAAAGTGAAAG
TGAAATGAAAAGCCCTGAGCTTGGATATTCAAAATAAATATCAAGACAGAAAGATTTGCA
CTTCTGGAGGAGCATAGATGATTGAGAACAATTTCTTTTCCAGTGATTCAGTTACTACAA
AGACAATGGATACTCTACAGCAGAGAAGAGTTACTGAGAAGTACATTGGTATTCCCATTT
TCTCATTTTTGCTTTAGTGAGCCCTTTTTAGATTTCTTCAACTCCCACGGCGGTAGGCAA
CCTTTATAGTGAGAGTGCAAAAATAAGGAAGACATTGATACTTCCTTATGGGGCATTGAG
ATTGATGACAAGCAACTGAAGGCAGTTCGTAGCCTCCTTTTTTCACTATCACACCCTTGA
AAGTCGGGATCATTCTGAACAGTGGTAGTGGTAAGGGCATCAGGGAGTACGCAATGGCAA
TAGGAACCCATTCTTGCGAGACGATTAACCCATTTGGGTATCGAATTCCCTGTCAGCTTG
TAACAGATATCATCACAAAAGTGATTGCAATTCTTCACAATCAAGTGATAACTATCACCA
TAATAATCTGCAGACAGGCGCTCCATGAACTCTCTA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC005239A_C01 KMC005239A_c01
         (1056 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAF99798.1|AC012463_15 T2E6.19 [Arabidopsis thaliana]              178  1e-43
ref|NP_564513.1| expressed protein; protein id: At1g47740.1, sup...   178  1e-43
gb|AAM65611.1| unknown [Arabidopsis thaliana]                         176  4e-43
ref|NP_568467.1| putative protein; protein id: At5g25170.1, supp...   102  1e-20
pir||E71444 probable EREBP-4 - Arabidopsis thaliana gi|2245108|e...   100  7e-20

>gb|AAF99798.1|AC012463_15 T2E6.19 [Arabidopsis thaliana]
          Length = 292

 Score =  178 bits (451), Expect = 1e-43
 Identities = 83/132 (62%), Positives = 102/132 (76%), Gaps = 2/132 (1%)
 Frame = -2

Query: 1055 REFMERLSADYYGDSYHLIVKNCNHFCDDICYKLTGNSIPKWVNRLARMGSYCHCVLPDA 876
            REFME ++  YYG+ YHLIVKNCNHFC D+CYKLTG  IPKWVNRLA++GS C C+LP++
Sbjct: 159  REFMEDMACSYYGNMYHLIVKNCNHFCQDVCYKLTGKKIPKWVNRLAQIGSVCSCILPES 218

Query: 875  LTTTTVQNDPDFQ--GCDSEKRRLRTAFSCLSSISMPHKEVSMSSLFLHSHYKGCLPPWE 702
            L  T V +DPD Q    ++EKR LR++FSCLSSISM  K++S SSLFL S  +GCLPPW+
Sbjct: 219  LKITAVCHDPDGQIPEEENEKRSLRSSFSCLSSISMRQKQLSTSSLFLQSPLRGCLPPWQ 278

Query: 701  LKKSKKGSLKQK 666
            LK+SK  S   K
Sbjct: 279  LKRSKSNSSSLK 290

>ref|NP_564513.1| expressed protein; protein id: At1g47740.1, supported by cDNA: 40816.
            [Arabidopsis thaliana] gi|19424079|gb|AAL87252.1| unknown
            protein [Arabidopsis thaliana] gi|21280795|gb|AAM45073.1|
            unknown protein [Arabidopsis thaliana]
          Length = 279

 Score =  178 bits (451), Expect = 1e-43
 Identities = 83/132 (62%), Positives = 102/132 (76%), Gaps = 2/132 (1%)
 Frame = -2

Query: 1055 REFMERLSADYYGDSYHLIVKNCNHFCDDICYKLTGNSIPKWVNRLARMGSYCHCVLPDA 876
            REFME ++  YYG+ YHLIVKNCNHFC D+CYKLTG  IPKWVNRLA++GS C C+LP++
Sbjct: 146  REFMEDMACSYYGNMYHLIVKNCNHFCQDVCYKLTGKKIPKWVNRLAQIGSVCSCILPES 205

Query: 875  LTTTTVQNDPDFQ--GCDSEKRRLRTAFSCLSSISMPHKEVSMSSLFLHSHYKGCLPPWE 702
            L  T V +DPD Q    ++EKR LR++FSCLSSISM  K++S SSLFL S  +GCLPPW+
Sbjct: 206  LKITAVCHDPDGQIPEEENEKRSLRSSFSCLSSISMRQKQLSTSSLFLQSPLRGCLPPWQ 265

Query: 701  LKKSKKGSLKQK 666
            LK+SK  S   K
Sbjct: 266  LKRSKSNSSSLK 277

>gb|AAM65611.1| unknown [Arabidopsis thaliana]
          Length = 251

 Score =  176 bits (447), Expect = 4e-43
 Identities = 82/132 (62%), Positives = 102/132 (77%), Gaps = 2/132 (1%)
 Frame = -2

Query: 1055 REFMERLSADYYGDSYHLIVKNCNHFCDDICYKLTGNSIPKWVNRLARMGSYCHCVLPDA 876
            REFME ++  YYG+ YHLIVKNCNHFC D+CYKLTG  IPKWVNRLA++GS C C+LP++
Sbjct: 118  REFMEDMACSYYGNMYHLIVKNCNHFCQDVCYKLTGKKIPKWVNRLAQIGSVCSCILPES 177

Query: 875  LTTTTVQNDPDFQ--GCDSEKRRLRTAFSCLSSISMPHKEVSMSSLFLHSHYKGCLPPWE 702
            L  T V +DPD Q    ++EKR LR++FSCLSSIS+  K++S SSLFL S  +GCLPPW+
Sbjct: 178  LKITAVCHDPDGQIPEEENEKRSLRSSFSCLSSISIRQKQLSTSSLFLQSPLRGCLPPWQ 237

Query: 701  LKKSKKGSLKQK 666
            LK+SK  S   K
Sbjct: 238  LKRSKSNSSSLK 249

>ref|NP_568467.1| putative protein; protein id: At5g25170.1, supported by cDNA: 263500.
            [Arabidopsis thaliana]
          Length = 218

 Score =  102 bits (254), Expect = 1e-20
 Identities = 56/122 (45%), Positives = 74/122 (59%), Gaps = 6/122 (4%)
 Frame = -2

Query: 1055 REFMERLSADYYGDSYHLIVKNCNHFCDDICYKLTGNSIPKWVNRLARMGSYCHCVLPDA 876
            R FME+L+ +Y G+SYHLI KNCNHFC+D+C +LT  SIP WVNRLAR G +C+CVLP  
Sbjct: 95   RVFMEKLAEEYSGNSYHLITKNCNHFCNDVCVQLTRRSIPSWVNRLARFGLFCNCVLPAE 154

Query: 875  LTTTTVQ--NDPDFQGCDSEKRRLRTAFSCLSSISMPHKEVSMSSLFLHS----HYKGCL 714
            L  T V+     + +  + EK++LR+     SS   P   +S S     S      + CL
Sbjct: 155  LNETKVRQVRSKEEKIPEVEKKKLRSR----SSRFPPGPSLSSSGSLNRSRRGERRRQCL 210

Query: 713  PP 708
            PP
Sbjct: 211  PP 212

>pir||E71444 probable EREBP-4 - Arabidopsis thaliana gi|2245108|emb|CAB10530.1|
            EREBP-4 like protein [Arabidopsis thaliana]
            gi|7268501|emb|CAB78752.1| EREBP-4 like protein
            [Arabidopsis thaliana]
          Length = 603

 Score = 99.8 bits (247), Expect = 7e-20
 Identities = 39/71 (54%), Positives = 56/71 (77%)
 Frame = -2

Query: 1055 REFMERLSADYYGDSYHLIVKNCNHFCDDICYKLTGNSIPKWVNRLARMGSYCHCVLPDA 876
            R +ME+LS  Y+GD+YHLI KNCNHF +++C +LTG  IP W+NRLAR+GS+C+C+LP++
Sbjct: 482  RSYMEKLSRKYHGDTYHLIAKNCNHFTEEVCLQLTGKPIPGWINRLARVGSFCNCLLPES 541

Query: 875  LTTTTVQNDPD 843
            +  T V   P+
Sbjct: 542  IQLTAVSALPE 552

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 887,253,094
Number of Sequences: 1393205
Number of extensions: 19513987
Number of successful extensions: 52242
Number of sequences better than 10.0: 55
Number of HSP's better than 10.0 without gapping: 48684
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 52122
length of database: 448,689,247
effective HSP length: 124
effective length of database: 275,931,827
effective search space used: 62636524729
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPD053d03_f AV773561 1 455 2 MPD035e07_f AV772407 289 760 3 MWM223a12_f AV768146 343 868 4 SPD017d04_f BP045332 477 847 5 MPDL071g07_f AV780155 494 1063 6 MPD080d07_f AV775251 497 920 7 SPD004e03_f BP044322 542 1034

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