Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005233A_C01 KMC005233A_c01
(612 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAF17578.1|AF202184_1 isoflavone reductase homolog 2 [Glycine... 53 5e-10
gb|AAL85023.1| putative NAD(P)H oxidoreductase, isoflavone reduc... 46 2e-08
ref|NP_195634.1| isoflavone reductase, putative; protein id: At4... 46 2e-08
gb|AAG22740.1|AF282850_1 allergenic isoflavone reductase-like pr... 47 2e-08
gb|AAC24001.1| isoflavone reductase related protein [Pyrus commu... 55 9e-07
>gb|AAF17578.1|AF202184_1 isoflavone reductase homolog 2 [Glycine max]
Length = 310
Score = 53.1 bits (126), Expect(2) = 5e-10
Identities = 60/160 (37%), Positives = 86/160 (53%), Gaps = 5/160 (3%)
Frame = +3
Query: 138 RAGHPTLAWVRQATLSDTAKAPVPVQFQGRAGCYFGSLGDLY--ES*VVRMKRDQGRWDV 311
+AGHPT VR++TLS+ AK+P+ F+G LGDLY +S V +K + DV
Sbjct: 28 KAGHPTFLLVRESTLSNPAKSPLIDNFKGLGVNLV--LGDLYDHQSLVSAIK----QVDV 81
Query: 312 VDSRSRWGGPGRLGRVRIKHHPP--TDRREAG*HKRGFLPISNSGNDV-RSHGLLLEPAR 482
V S +G +++ + +EAG K+ F P S GNDV R+H +EPA+
Sbjct: 82 VIS--------TVGHLQLADQDKIISAIKEAGNVKK-FYP-SEFGNDVDRTHA--VEPAK 129
Query: 483 ICDLQAKAHSSSSLFEAEGIPLYYMSPCNYFAWLFSPHIS 602
KA ++ EAEGIP Y+S N+FA F P++S
Sbjct: 130 SA-FATKAKVRRAI-EAEGIPFTYVS-SNFFAGYFLPNLS 166
Score = 32.3 bits (72), Expect(2) = 5e-10
Identities = 14/14 (100%), Positives = 14/14 (100%)
Frame = +1
Query: 70 KSKILFIGGTGYIG 111
KSKILFIGGTGYIG
Sbjct: 6 KSKILFIGGTGYIG 19
>gb|AAL85023.1| putative NAD(P)H oxidoreductase, isoflavone reductase [Arabidopsis
thaliana]
Length = 308
Score = 46.2 bits (108), Expect(2) = 2e-08
Identities = 57/157 (36%), Positives = 77/157 (48%), Gaps = 2/157 (1%)
Frame = +3
Query: 138 RAGHPTLAWVRQATLSDTAKAPVPVQFQGRAGCYFGSLGDL--YES*VVRMKRDQGRWDV 311
R+GHPTL VR +TL+ +++ F+ G F LGDL + S V +K+ DV
Sbjct: 26 RSGHPTLVLVRNSTLTSPSRSSTIENFK-NLGVQF-LLGDLDDHTSLVNSIKQA----DV 79
Query: 312 VDSRSRWGGPGRLGRVRIKHHPPTDRREAG*HKRGFLPISNSGNDVRSHGLLLEPARICD 491
V S G LG ++ + +EAG KR F P S GNDV +EPA+
Sbjct: 80 VISTV---GHSLLGH---QYKIISAIKEAGNVKR-FFP-SEFGNDV-DRVFTVEPAK--S 128
Query: 492 LQAKAHSSSSLFEAEGIPLYYMSPCNYFAWLFSPHIS 602
A EAEGIP Y+S CN+FA F P ++
Sbjct: 129 AYATKAKIRRTIEAEGIPYTYVS-CNFFAGYFLPTLA 164
Score = 34.3 bits (77), Expect(2) = 2e-08
Identities = 15/17 (88%), Positives = 15/17 (88%)
Frame = +1
Query: 61 MAEKSKILFIGGTGYIG 111
M KSKILFIGGTGYIG
Sbjct: 1 MTSKSKILFIGGTGYIG 17
>ref|NP_195634.1| isoflavone reductase, putative; protein id: At4g39230.1, supported
by cDNA: gi_19310584 [Arabidopsis thaliana]
gi|7484776|pir||T08571 2'-hydroxyisoflavone reductase
(EC 1.3.1.45) T22F8.130 - Arabidopsis thaliana
gi|4914435|emb|CAB43638.1| NAD(P)H oxidoreductase,
isoflavone reductase-like protein [Arabidopsis thaliana]
gi|7270906|emb|CAB80586.1| NAD(P)H oxidoreductase,
isoflavone reductase-like protein [Arabidopsis thaliana]
gi|23297378|gb|AAN12954.1| putative NAD(P)H
oxidoreductase, isoflavone reductase [Arabidopsis
thaliana]
Length = 308
Score = 46.2 bits (108), Expect(2) = 2e-08
Identities = 57/157 (36%), Positives = 77/157 (48%), Gaps = 2/157 (1%)
Frame = +3
Query: 138 RAGHPTLAWVRQATLSDTAKAPVPVQFQGRAGCYFGSLGDL--YES*VVRMKRDQGRWDV 311
R+GHPTL VR +TL+ +++ F+ G F LGDL + S V +K+ DV
Sbjct: 26 RSGHPTLVLVRNSTLTSPSRSSTIENFK-NLGVQF-LLGDLDDHTSLVNSIKQA----DV 79
Query: 312 VDSRSRWGGPGRLGRVRIKHHPPTDRREAG*HKRGFLPISNSGNDVRSHGLLLEPARICD 491
V S G LG ++ + +EAG KR F P S GNDV +EPA+
Sbjct: 80 VISTV---GHSLLGH---QYKIISAIKEAGNVKR-FFP-SEFGNDV-DRVFTVEPAK--S 128
Query: 492 LQAKAHSSSSLFEAEGIPLYYMSPCNYFAWLFSPHIS 602
A EAEGIP Y+S CN+FA F P ++
Sbjct: 129 AYATKAKIRRTIEAEGIPYTYVS-CNFFAGYFLPTLA 164
Score = 34.3 bits (77), Expect(2) = 2e-08
Identities = 15/17 (88%), Positives = 15/17 (88%)
Frame = +1
Query: 61 MAEKSKILFIGGTGYIG 111
M KSKILFIGGTGYIG
Sbjct: 1 MTSKSKILFIGGTGYIG 17
>gb|AAG22740.1|AF282850_1 allergenic isoflavone reductase-like protein Bet v 6.0102 [Betula
pendula]
Length = 308
Score = 46.6 bits (109), Expect(2) = 2e-08
Identities = 58/160 (36%), Positives = 78/160 (48%), Gaps = 5/160 (3%)
Frame = +3
Query: 138 RAGHPTLAWVRQATLSDTAKAPVPVQFQGRAGCYFGSLGDLY--ES*VVRMKRDQGRWDV 311
++GHPT A VR++T+SD K + +F+G GDLY ES V K + DV
Sbjct: 26 KSGHPTFALVRESTVSDPVKGKLVEKFKGLGVTLLH--GDLYDHESLVKAFK----QVDV 79
Query: 312 VDSRSRWGGPGRLGRVRIKHHPP--TDRREAG*HKRGFLPISNSGNDV-RSHGLLLEPAR 482
V S +G +++ +EAG KR F P S GNDV R H +EPA+
Sbjct: 80 VIS--------TVGHLQLADQVKIIAAIKEAGNIKR-FFP-SEFGNDVDRVHA--VEPAK 127
Query: 483 ICDLQAKAHSSSSLFEAEGIPLYYMSPCNYFAWLFSPHIS 602
KA EAEGIP Y+S N+FA F P ++
Sbjct: 128 TA-FATKAEIRRKT-EAEGIPYTYVS-SNFFAGYFLPTLA 164
Score = 33.5 bits (75), Expect(2) = 2e-08
Identities = 15/17 (88%), Positives = 15/17 (88%)
Frame = +1
Query: 61 MAEKSKILFIGGTGYIG 111
MA KSKIL IGGTGYIG
Sbjct: 1 MAHKSKILIIGGTGYIG 17
>gb|AAC24001.1| isoflavone reductase related protein [Pyrus communis]
Length = 308
Score = 54.7 bits (130), Expect = 9e-07
Identities = 64/158 (40%), Positives = 84/158 (52%), Gaps = 3/158 (1%)
Frame = +3
Query: 138 RAGHPTLAWVRQATLSDTAKAPVPVQFQGRAGCYFGSLGDLY--ES*VVRMKRDQGRWDV 311
+AG+PT VR+A+LSD AK+ V F+ G F LGDLY ES V +K + DV
Sbjct: 26 KAGYPTYVLVREASLSDPAKSKVIENFKA-LGVNF-VLGDLYDHESLVKAIK----QVDV 79
Query: 312 VDSRSRWGGPGRLGRVRIKHHPPTDRREAG*HKRGFLPISNSGNDV-RSHGLLLEPARIC 488
V S G G++ +EAG KR F P S GNDV RSH +EPA+
Sbjct: 80 VISTVGHGQLADQGKI------IAAIKEAGNVKR-FFP-SEFGNDVDRSHA--VEPAKSA 129
Query: 489 DLQAKAHSSSSLFEAEGIPLYYMSPCNYFAWLFSPHIS 602
+ KA ++ EAEGIP Y+S N+FA F P ++
Sbjct: 130 -FETKAKIRRAV-EAEGIPYTYVS-SNFFAGYFLPTLN 164
Score = 35.4 bits (80), Expect = 0.59
Identities = 55/188 (29%), Positives = 83/188 (43%), Gaps = 4/188 (2%)
Frame = +1
Query: 61 MAEKSKILFIGGTGYIG-ITHRRSKRLELDTQLW--HGSGKPPSLTLLKLQFLYNFKVV- 228
MA KS+ILFIGGTGYIG S + T + S P+ K + + NFK +
Sbjct: 1 MASKSQILFIGGTGYIGKFIVEASAKAGYPTYVLVREASLSDPA----KSKVIENFKALG 56
Query: 229 LDVTLGRWGICTNHE**G*NVIKAGGMWLTLAAGGVALADLAESGSSIIRRPIEGRPVNI 408
++ LG +HE +++KA + + V LA+ G I G N+
Sbjct: 57 VNFVLGD---LYDHE----SLVKAIKQ-VDVVISTVGHGQLADQGKIIAAIKEAG---NV 105
Query: 409 KEDFCQSAIRATTLDRTACC*SQQESAICKPKPTVRRHYLKPKASHYTICLHATTSPGYF 588
K F +DR+ + + + K +RR ++ + YT + + GYF
Sbjct: 106 KRFFPSEF--GNDVDRSHAV--EPAKSAFETKAKIRR-AVEAEGIPYTY-VSSNFFAGYF 159
Query: 589 LPTLAQPG 612
LPTL QPG
Sbjct: 160 LPTLNQPG 167
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 577,225,969
Number of Sequences: 1393205
Number of extensions: 13298186
Number of successful extensions: 38480
Number of sequences better than 10.0: 55
Number of HSP's better than 10.0 without gapping: 36454
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 38444
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 24568846532
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
