>KMC005216A_C01 KMC005216A_c01
cacactctctctatctctttttatcttctctcattctccattCTCACACTCCCTCTCTCT
GCAATCCGCAACCATGTCTCACTTCGGAAGGTCCGGTCCCCCTGTACATCACCGACACCT
ACTCCCTCCTCGTTCTCAACATCACCTTCCGCACAACTGCTGATGACTTGTTCCCTCTCT
TCGATAAGTATGGGAAAGTTGTCGACATCTTCATCCCCAAAGATCGAAGGACTGGGGAGT
CGAGGGGTTTCGCGTTTGTGCGTTACAAGTATGCTGATGAGGCTTCAAAGGCTGTTGATA
GGCTTGATGGAAGAATGGTTGATGGTCGTGAAATAACTGTTCAGTTTGCTAAGTATGGGC
CTAATGCTGAGAGGATTCACAAAGGTAGAATTATTGAATCAGAGGAAAGAGCTAAGCAGG
GGTCACGGAGCCGTAGCCCCAGGAGAAGGCATCGTGATGATCACAGATCCAGCAGAGACA
GGGATTACAGGAGGAGAAGCCGCAGCAGAAGTTATGATAAGTATGAACGTGGTGATAGGC
ACCGTGGGAGAGACAGGGATTATCGTCGCCGAAGCAGGAGCCGCAGTGCCAGTCTGGAT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC005216A_C01 KMC005216A_c01
         (599 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||T09704 probable arginine/serine-rich splicing factor - alfa...   273  3e-74
emb|CAC03600.1| splicing factor SC35 [Arabidopsis thaliana]           234  2e-62
ref|NP_201225.1| arginine/serine-rich splicing factor SC35; prot...   234  2e-62
dbj|BAC10847.1| putative splicing factor, arginine/serine-rich 2...   182  6e-46
gb|AAH45229.1| Unknown (protein for MGC:53177) [Xenopus laevis]       129  4e-29

>pir||T09704 probable arginine/serine-rich splicing factor - alfalfa
           gi|3334756|emb|CAA76346.1| putative arginine/serine-rich
           splicing factor [Medicago sativa]
          Length = 286

 Score =  273 bits (699), Expect(2) = 3e-74
 Identities = 143/166 (86%), Positives = 154/166 (92%), Gaps = 1/166 (0%)
 Frame = +3

Query: 99  PLYITDTYSLLVLNITFRTTADDLFPLFDKYGKVVDIFIPKDRRTGESRGFAFVRYKYAD 278
           P  I+DTYSLLVLNITFRTTADDLFPLFDKYGKVVDIFIPKDRRTGESRGFAFVRYKYAD
Sbjct: 9   PPDISDTYSLLVLNITFRTTADDLFPLFDKYGKVVDIFIPKDRRTGESRGFAFVRYKYAD 68

Query: 279 EASKAVDRLDGRMVDGREITVQFAKYGPNAERIHKGRIIESEERAKQGSRSRSP-RRRHR 455
           EASKAVDRLDGRMVDGREITVQFAKYGPNAERI KGRIIE+  R+K  SRSRSP +RRHR
Sbjct: 69  EASKAVDRLDGRMVDGREITVQFAKYGPNAERIQKGRIIETSPRSK-SSRSRSPSKRRHR 127

Query: 456 DDHRSSRDRDYRRRSRSRSYDKYERGDRHRGRDRDYRRRSRSRSAS 593
           DD+   +++DYRRRSRSRSYD++ER DRHRGRDRD+RRRSRSRSAS
Sbjct: 128 DDY---KEKDYRRRSRSRSYDRHER-DRHRGRDRDHRRRSRSRSAS 169

 Score = 26.9 bits (58), Expect(2) = 3e-74
 Identities = 10/10 (100%), Positives = 10/10 (100%)
 Frame = +2

Query: 74  MSHFGRSGPP 103
           MSHFGRSGPP
Sbjct: 1   MSHFGRSGPP 10

>emb|CAC03600.1| splicing factor SC35 [Arabidopsis thaliana]
          Length = 303

 Score =  234 bits (598), Expect(2) = 2e-62
 Identities = 125/190 (65%), Positives = 144/190 (75%), Gaps = 23/190 (12%)
 Frame = +3

Query: 99  PLYITDTYSLLVLNITFRTTADDLFPLFDKYGKVVDIFIPKDRRTGESRGFAFVRYKYAD 278
           P  I+DTYSLLVLNITFRTTADDL+PLF KYGKVVD+FIP+DRRTG+SRGFAFVRYKY D
Sbjct: 9   PPDISDTYSLLVLNITFRTTADDLYPLFAKYGKVVDVFIPRDRRTGDSRGFAFVRYKYKD 68

Query: 279 EASKAVDRLDGRMVDGREITVQFAKYGPNAERIHKGRIIE-------------------- 398
           EA KAV+RLDGR+VDGREITVQFAKYGPNAE+I KGR++E                    
Sbjct: 69  EAHKAVERLDGRVVDGREITVQFAKYGPNAEKISKGRVVEPPPKSRRSRSRSPRRSRSPR 128

Query: 399 ---SEERAKQGSRSRSPRRRHRDDHRSSRDRDYRRRSRSRSYDKYERGDRHRGRDRDYRR 569
              S  R +   RSRSPRRR RDD+   R++DYR+RSRSRSYD   R +RH  +DRD+RR
Sbjct: 129 RSRSPPRRRSPRRSRSPRRRSRDDY---REKDYRKRSRSRSYD---RRERHEEKDRDHRR 182

Query: 570 RSRSRSASLD 599
           R+RSRSAS D
Sbjct: 183 RTRSRSASPD 192

 Score = 26.9 bits (58), Expect(2) = 2e-62
 Identities = 10/10 (100%), Positives = 10/10 (100%)
 Frame = +2

Query: 74  MSHFGRSGPP 103
           MSHFGRSGPP
Sbjct: 1   MSHFGRSGPP 10

>ref|NP_201225.1| arginine/serine-rich splicing factor SC35; protein id: At5g64200.1,
           supported by cDNA: gi_17380867 [Arabidopsis thaliana]
           gi|17380868|gb|AAL36246.1| unknown protein [Arabidopsis
           thaliana] gi|21689657|gb|AAM67450.1| unknown protein
           [Arabidopsis thaliana] gi|22022546|gb|AAM83231.1|
           AT5g64200/MSJ1_4 [Arabidopsis thaliana]
           gi|23505861|gb|AAN28790.1| At5g64200/MSJ1_4 [Arabidopsis
           thaliana]
          Length = 303

 Score =  234 bits (598), Expect(2) = 2e-62
 Identities = 125/190 (65%), Positives = 144/190 (75%), Gaps = 23/190 (12%)
 Frame = +3

Query: 99  PLYITDTYSLLVLNITFRTTADDLFPLFDKYGKVVDIFIPKDRRTGESRGFAFVRYKYAD 278
           P  I+DTYSLLVLNITFRTTADDL+PLF KYGKVVD+FIP+DRRTG+SRGFAFVRYKY D
Sbjct: 9   PPDISDTYSLLVLNITFRTTADDLYPLFAKYGKVVDVFIPRDRRTGDSRGFAFVRYKYKD 68

Query: 279 EASKAVDRLDGRMVDGREITVQFAKYGPNAERIHKGRIIE-------------------- 398
           EA KAV+RLDGR+VDGREITVQFAKYGPNAE+I KGR++E                    
Sbjct: 69  EAHKAVERLDGRVVDGREITVQFAKYGPNAEKISKGRVVEPPPKSRRSRSRSPRRSRSPR 128

Query: 399 ---SEERAKQGSRSRSPRRRHRDDHRSSRDRDYRRRSRSRSYDKYERGDRHRGRDRDYRR 569
              S  R +   RSRSPRRR RDD+   R++DYR+RSRSRSYD   R +RH  +DRD+RR
Sbjct: 129 RSRSPPRRRSPRRSRSPRRRSRDDY---REKDYRKRSRSRSYD---RRERHEEKDRDHRR 182

Query: 570 RSRSRSASLD 599
           R+RSRSAS D
Sbjct: 183 RTRSRSASPD 192

 Score = 26.9 bits (58), Expect(2) = 2e-62
 Identities = 10/10 (100%), Positives = 10/10 (100%)
 Frame = +2

Query: 74  MSHFGRSGPP 103
           MSHFGRSGPP
Sbjct: 1   MSHFGRSGPP 10

>dbj|BAC10847.1| putative splicing factor, arginine/serine-rich 2 (Splicing factor
           SC35) [Oryza sativa (japonica cultivar-group)]
          Length = 190

 Score =  182 bits (462), Expect(2) = 6e-46
 Identities = 105/168 (62%), Positives = 119/168 (70%), Gaps = 2/168 (1%)
 Frame = +3

Query: 90  GPVPLYITDTYSLLVLNITFRTTADDLFPLFDKYGKVVDIFIPKDRRTGESRGFAFVRYK 269
           GP P  I DTYSLLVLNITFRTTADDL PLF+KYG+VVDI+IP+DRRTG+SRGFAFVRYK
Sbjct: 8   GPPP--IRDTYSLLVLNITFRTTADDLSPLFEKYGEVVDIYIPRDRRTGDSRGFAFVRYK 65

Query: 270 YADEASKAVDRLDGRMVDGREITVQFAKYGPNAERIHKGRIIESEERAKQGSRSRSPRRR 449
           Y DEA KAVDRLDGR+VDGREI VQFAKYGPNAER+   R    ++  ++G  S SP RR
Sbjct: 66  YEDEAQKAVDRLDGRVVDGREIMVQFAKYGPNAERM--SRSYTPDDYRRRGRDSVSPARR 123

Query: 450 --HRDDHRSSRDRDYRRRSRSRSYDKYERGDRHRGRDRDYRRRSRSRS 587
              R   RS    DYR+R + R+    E     R    D  RRSRS S
Sbjct: 124 SLSRSRSRSYSPDDYRKRGKHRTPSSQEGSPVKR---YDEPRRSRSPS 168

 Score = 23.9 bits (50), Expect(2) = 6e-46
 Identities = 9/10 (90%), Positives = 9/10 (90%)
 Frame = +2

Query: 74  MSHFGRSGPP 103
           MS FGRSGPP
Sbjct: 1   MSRFGRSGPP 10

>gb|AAH45229.1| Unknown (protein for MGC:53177) [Xenopus laevis]
          Length = 215

 Score =  129 bits (323), Expect = 4e-29
 Identities = 76/174 (43%), Positives = 104/174 (59%), Gaps = 3/174 (1%)
 Frame = +3

Query: 84  SEGPVPLYITDTYSLLVLNITFRTTADDLFPLFDKYGKVVDIFIPKDRRTGESRGFAFVR 263
           S G  P  +    SL V N+T+RT+ + L  +F+KYG+V D++IP+DR T ESRGFAFVR
Sbjct: 2   SYGRPPPDVEGMTSLKVDNLTYRTSPETLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVR 61

Query: 264 YKYADEASKAVDRLDGRMVDGREITVQFAKYGPNAERIHKGRIIESEERAKQGSRSRSPR 443
           +    +A  A+D +DG ++DGRE+ VQ A+YG   +  H  R          G RSRSPR
Sbjct: 62  FHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHGRRGPAPRRYGDYGRRSRSPR 121

Query: 444 RRHRDDHRS---SRDRDYRRRSRSRSYDKYERGDRHRGRDRDYRRRSRSRSASL 596
           RR R   RS   SR R   R SRS+S+ +     R R + R   RRS+S+S+S+
Sbjct: 122 RRRRSRSRSKSRSRSRSRSRHSRSKSHSRTRSRTRSRSKSRS-ARRSKSKSSSV 174

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 538,338,939
Number of Sequences: 1393205
Number of extensions: 12847191
Number of successful extensions: 87378
Number of sequences better than 10.0: 3710
Number of HSP's better than 10.0 without gapping: 60899
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 78471
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23426109484
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MWM005f10_f AV764732 1 422 2 MPD031b11_f AV772110 26 473 3 MF003b08_f BP028377 108 599

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