Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005158A_C03 KMC005158A_c03
(1446 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|P12858|G3PA_PEA Glyceraldehyde 3-phosphate dehydrogenase A, c... 733 0.0
emb|CAA33264.1| precursor (AA -68 to 337) [Pisum sativum] 730 0.0
ref|NP_172750.1| putative calcium-binding protein, calreticulin;... 693 0.0
sp|P19866|G3PA_SPIOL Glyceraldehyde 3-phosphate dehydrogenase A,... 687 0.0
sp|P25856|G3PA_ARATH Glyceraldehyde 3-phosphate dehydrogenase A,... 683 0.0
>sp|P12858|G3PA_PEA Glyceraldehyde 3-phosphate dehydrogenase A, chloroplast precursor
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit A) gi|66025|pir||DEPMNA
glyceraldehyde-3-phosphate dehydrogenase (NADP)
(phosphorylating) (EC 1.2.1.13) A precursor, chloroplast
- garden pea gi|12159|emb|CAA36396.1|
glyceraldehyde-3-phosphate dehydrogenase [Pisum sativum]
Length = 405
Score = 733 bits (1893), Expect = 0.0
Identities = 375/405 (92%), Positives = 388/405 (95%), Gaps = 4/405 (0%)
Frame = -3
Query: 1417 MASATLSLAKPALKANGKGFSDFSGLR--SANLPFSRKTSEDFHSVIAFQTYAVGSSGGY 1244
MASAT S+AKPA+KANGKGFS+FSGLR S +LPFSRK+S+DFHS++ FQT AVGSSGG+
Sbjct: 1 MASATFSVAKPAIKANGKGFSEFSGLRNSSRHLPFSRKSSDDFHSLVTFQTNAVGSSGGH 60
Query: 1243 KKG-VVEAK-LKVAINGFGRIGRNFLRCWHGRKDSPLDVIAINDTGGVKQASHLLKYDST 1070
KK VVEAK LKVAINGFGRIGRNFLRCWHGRKDSPLDVIAINDTGGVKQASHLLKYDST
Sbjct: 61 KKSLVVEAKQLKVAINGFGRIGRNFLRCWHGRKDSPLDVIAINDTGGVKQASHLLKYDST 120
Query: 1069 LGIFGADVKPVGTDGISVDGKVIKVVSDRNPANLPWGELGIDLVIEGTGVFVDREGAGKH 890
LGIF ADVKPVGTDGISVDGKVIKVVSDRNPANLPW ELGIDLVIEGTGVFVDREGAG+H
Sbjct: 121 LGIFDADVKPVGTDGISVDGKVIKVVSDRNPANLPWKELGIDLVIEGTGVFVDREGAGRH 180
Query: 889 IQAGAKKVLITAPGKGDIPTYVVGVNADAYSHDDDIISNASCTTNCLAPFVKVLDQKFGI 710
I AGAKKVLITAPGKGDIPTYVVGVNADAY+H DDIISNASCTTNCLAPFVKVLDQKFGI
Sbjct: 181 ITAGAKKVLITAPGKGDIPTYVVGVNADAYTHADDIISNASCTTNCLAPFVKVLDQKFGI 240
Query: 709 IKGTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPTLKGKLNGI 530
IKGTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPTLKGKLNGI
Sbjct: 241 IKGTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPTLKGKLNGI 300
Query: 529 ALRVPTPNVSVVDLVVQVTKKTFAEEVNAAFRESADNELNGILSVCDEPLVSVDFRCTDV 350
ALRVPTPNVSVVDLVVQV+KKTFAEEVN AFRESA EL GILSVCDEPLVSVDFRCTDV
Sbjct: 301 ALRVPTPNVSVVDLVVQVSKKTFAEEVNEAFRESAAKELTGILSVCDEPLVSVDFRCTDV 360
Query: 349 SSTVDSSLTMVMGDDMVKVIAWYDNEWGYSQRVVDLADIVANNWK 215
SSTVDSSLTMVMGDD+VKVIAWYDNEWGYSQRVVDLADIVANNWK
Sbjct: 361 SSTVDSSLTMVMGDDLVKVIAWYDNEWGYSQRVVDLADIVANNWK 405
>emb|CAA33264.1| precursor (AA -68 to 337) [Pisum sativum]
Length = 405
Score = 730 bits (1885), Expect = 0.0
Identities = 374/405 (92%), Positives = 387/405 (95%), Gaps = 4/405 (0%)
Frame = -3
Query: 1417 MASATLSLAKPALKANGKGFSDFSGLR--SANLPFSRKTSEDFHSVIAFQTYAVGSSGGY 1244
MASAT S+AKPA+KANGKGFS+FSGLR S +LPFSRK+S+DFHS++ FQT AVGSSGG+
Sbjct: 1 MASATFSVAKPAIKANGKGFSEFSGLRNSSRHLPFSRKSSDDFHSLVTFQTNAVGSSGGH 60
Query: 1243 KKG-VVEAK-LKVAINGFGRIGRNFLRCWHGRKDSPLDVIAINDTGGVKQASHLLKYDST 1070
KK VVEAK LKVAINGFGRIGRNFLRCWHGRKDSPLDVIAINDTGGVKQASHLLKYDST
Sbjct: 61 KKSLVVEAKQLKVAINGFGRIGRNFLRCWHGRKDSPLDVIAINDTGGVKQASHLLKYDST 120
Query: 1069 LGIFGADVKPVGTDGISVDGKVIKVVSDRNPANLPWGELGIDLVIEGTGVFVDREGAGKH 890
LGIF ADVKPVGTDGISVDGKVIKVVSDRNPANLPW ELGIDLVIEGTGVFVDREGAG+H
Sbjct: 121 LGIFDADVKPVGTDGISVDGKVIKVVSDRNPANLPWKELGIDLVIEGTGVFVDREGAGRH 180
Query: 889 IQAGAKKVLITAPGKGDIPTYVVGVNADAYSHDDDIISNASCTTNCLAPFVKVLDQKFGI 710
I AGAKKVLITAP KGDIPTYVVGVNADAY+H DDIISNASCTTNCLAPFVKVLDQKFGI
Sbjct: 181 ITAGAKKVLITAPRKGDIPTYVVGVNADAYTHADDIISNASCTTNCLAPFVKVLDQKFGI 240
Query: 709 IKGTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPTLKGKLNGI 530
IKGTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPTLKGKLNGI
Sbjct: 241 IKGTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPTLKGKLNGI 300
Query: 529 ALRVPTPNVSVVDLVVQVTKKTFAEEVNAAFRESADNELNGILSVCDEPLVSVDFRCTDV 350
ALRVPTPNVSVVDLVVQV+KKTFAEEVN AFRESA EL GILSVCDEPLVSVDFRCTDV
Sbjct: 301 ALRVPTPNVSVVDLVVQVSKKTFAEEVNEAFRESAAKELTGILSVCDEPLVSVDFRCTDV 360
Query: 349 SSTVDSSLTMVMGDDMVKVIAWYDNEWGYSQRVVDLADIVANNWK 215
SSTVDSSLTMVMGDD+VKVIAWYDNEWGYSQRVVDLADIVANNWK
Sbjct: 361 SSTVDSSLTMVMGDDLVKVIAWYDNEWGYSQRVVDLADIVANNWK 405
>ref|NP_172750.1| putative calcium-binding protein, calreticulin; protein id:
At1g12900.1 [Arabidopsis thaliana]
gi|25284345|pir||F86262 F13K23.15 protein - Arabidopsis
thaliana gi|8698736|gb|AAF78494.1|AC012187_14 Strong
similarity to GAPDH subunit A from Pisum sativum
gb|X15190 and contains a GAPDH PF|00044 domain. ESTs
gb|T42920, gb|T43410, gb|T46101, gb|T04006, gb|T20630,
gb|Z34677, gb|T46805, gb|N37754, gb|N37754, gb|Z26072,
gb|H37169, gb|H76419, gb|T20834, gb|T21557, gb|AA713258,
gb|T04005, gb|AI099909, gb|Z34793 come from this gene.
[Arabidopsis thaliana]
Length = 399
Score = 693 bits (1788), Expect = 0.0
Identities = 346/403 (85%), Positives = 378/403 (92%), Gaps = 2/403 (0%)
Frame = -3
Query: 1417 MASATLSLAKPALKANGKGFSDFSGLRSAN-LPFSRKTSED-FHSVIAFQTYAVGSSGGY 1244
MASAT S+AKP+L+ GFS+FSGLR+++ LPF++++S D F S ++FQT A+ S+GGY
Sbjct: 1 MASATFSVAKPSLQ----GFSEFSGLRNSSALPFAKRSSSDEFVSFVSFQTSAMRSNGGY 56
Query: 1243 KKGVVEAKLKVAINGFGRIGRNFLRCWHGRKDSPLDVIAINDTGGVKQASHLLKYDSTLG 1064
+KGV EAK+KVAINGFGRIGRNFLRCWHGRKDSPLDV+ INDTGGVKQASHLLKYDSTLG
Sbjct: 57 RKGVTEAKIKVAINGFGRIGRNFLRCWHGRKDSPLDVVVINDTGGVKQASHLLKYDSTLG 116
Query: 1063 IFGADVKPVGTDGISVDGKVIKVVSDRNPANLPWGELGIDLVIEGTGVFVDREGAGKHIQ 884
IF ADVKP G +SVDGK+IK+VSDRNP+NLPWGELGIDLVIEGTGVFVDR+GAGKH+Q
Sbjct: 117 IFDADVKPSGDSALSVDGKIIKIVSDRNPSNLPWGELGIDLVIEGTGVFVDRDGAGKHLQ 176
Query: 883 AGAKKVLITAPGKGDIPTYVVGVNADAYSHDDDIISNASCTTNCLAPFVKVLDQKFGIIK 704
AGAKKVLITAPGKGDIPTYVVGVNA+ YSH+D IISNASCTTNCLAPFVKVLDQKFGIIK
Sbjct: 177 AGAKKVLITAPGKGDIPTYVVGVNAELYSHEDTIISNASCTTNCLAPFVKVLDQKFGIIK 236
Query: 703 GTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPTLKGKLNGIAL 524
GTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLP LKGKLNGIAL
Sbjct: 237 GTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPNLKGKLNGIAL 296
Query: 523 RVPTPNVSVVDLVVQVTKKTFAEEVNAAFRESADNELNGILSVCDEPLVSVDFRCTDVSS 344
RVPTPNVSVVDLVVQV+KKTFAEEVNAAFR++A+ EL GIL VCDEPLVSVDFRC+DVSS
Sbjct: 297 RVPTPNVSVVDLVVQVSKKTFAEEVNAAFRDAAEKELKGILDVCDEPLVSVDFRCSDVSS 356
Query: 343 TVDSSLTMVMGDDMVKVIAWYDNEWGYSQRVVDLADIVANNWK 215
T+DSSLTMVMGDDMVKVIAWYDNEWGYSQRVVDLADIVANNWK
Sbjct: 357 TIDSSLTMVMGDDMVKVIAWYDNEWGYSQRVVDLADIVANNWK 399
>sp|P19866|G3PA_SPIOL Glyceraldehyde 3-phosphate dehydrogenase A, chloroplast precursor
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit A) gi|7431539|pir||T09012
glyceraldehyde-3-phosphate dehydrogenase (NADP)
(phosphorylating) (EC 1.2.1.13) chain A precursor,
chloroplast - spinach chloroplast
gi|2529370|gb|AAD10217.1| NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A [Spinacia
oleracea]
Length = 401
Score = 687 bits (1772), Expect = 0.0
Identities = 341/401 (85%), Positives = 364/401 (90%)
Frame = -3
Query: 1417 MASATLSLAKPALKANGKGFSDFSGLRSANLPFSRKTSEDFHSVIAFQTYAVGSSGGYKK 1238
MAS LS+A P+L+ KGFS+FSGL +++LPF RK S+D + ++FQT AVG +
Sbjct: 1 MASNMLSIANPSLRVYNKGFSEFSGLHTSSLPFGRKGSDDLMAFVSFQTNAVGGKRSSQN 60
Query: 1237 GVVEAKLKVAINGFGRIGRNFLRCWHGRKDSPLDVIAINDTGGVKQASHLLKYDSTLGIF 1058
GVVEAKLKVAINGFGRIGRNFLRCWHGRKDSPLDV+ INDTGGVKQASHLLKYDS LG F
Sbjct: 61 GVVEAKLKVAINGFGRIGRNFLRCWHGRKDSPLDVVVINDTGGVKQASHLLKYDSILGTF 120
Query: 1057 GADVKPVGTDGISVDGKVIKVVSDRNPANLPWGELGIDLVIEGTGVFVDREGAGKHIQAG 878
ADVK G ISVDGKVIKVVSDRNP NLPWG++GIDLVIEGTGVFVDR+GAGKH+QAG
Sbjct: 121 DADVKTAGDSAISVDGKVIKVVSDRNPVNLPWGDMGIDLVIEGTGVFVDRDGAGKHLQAG 180
Query: 877 AKKVLITAPGKGDIPTYVVGVNADAYSHDDDIISNASCTTNCLAPFVKVLDQKFGIIKGT 698
AKKVLITAPGKGDIPTYVVGVN + Y+H D IISNASCTTNCLAPFVKVLDQKFGIIKGT
Sbjct: 181 AKKVLITAPGKGDIPTYVVGVNEEGYTHADTIISNASCTTNCLAPFVKVLDQKFGIIKGT 240
Query: 697 MTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPTLKGKLNGIALRV 518
MTTTHSYTGDQRLLDASHRDLRRARAA LNIVPTSTGAAKAVALVLP LKGKLNGIALRV
Sbjct: 241 MTTTHSYTGDQRLLDASHRDLRRARAACLNIVPTSTGAAKAVALVLPNLKGKLNGIALRV 300
Query: 517 PTPNVSVVDLVVQVTKKTFAEEVNAAFRESADNELNGILSVCDEPLVSVDFRCTDVSSTV 338
PTPNVSVVDLVVQV+KKTFAEEVNAAFRESADNEL GILSVCDEPLVS+DFRCTDVSST+
Sbjct: 301 PTPNVSVVDLVVQVSKKTFAEEVNAAFRESADNELKGILSVCDEPLVSIDFRCTDVSSTI 360
Query: 337 DSSLTMVMGDDMVKVIAWYDNEWGYSQRVVDLADIVANNWK 215
DSSLTMVMGDDMVKVIAWYDNEWGYSQRVVDLADIVAN W+
Sbjct: 361 DSSLTMVMGDDMVKVIAWYDNEWGYSQRVVDLADIVANKWQ 401
>sp|P25856|G3PA_ARATH Glyceraldehyde 3-phosphate dehydrogenase A, chloroplast precursor
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit A) gi|11994300|dbj|BAB01730.1|
glyceralehyde-3-phosphate dehydrogenase subunit
[Arabidopsis thaliana]
gi|16226559|gb|AAL16200.1|AF428431_1 AT3g26650/MLJ15_5
[Arabidopsis thaliana] gi|16604418|gb|AAL24215.1|
AT3g26650/MLJ15_5 [Arabidopsis thaliana]
gi|16648728|gb|AAL25556.1| AT3g26650/MLJ15_5 [Arabidopsis
thaliana] gi|19715651|gb|AAL91645.1| AT3g26650/MLJ15_5
[Arabidopsis thaliana] gi|22655450|gb|AAM98317.1|
At3g26650/MLJ15_5 [Arabidopsis thaliana]
Length = 396
Score = 683 bits (1763), Expect = 0.0
Identities = 346/404 (85%), Positives = 371/404 (91%), Gaps = 3/404 (0%)
Frame = -3
Query: 1417 MASATLSLAKPALKANGKGFSDFSGLRS--ANLPFSRKTSED-FHSVIAFQTYAVGSSGG 1247
MAS T S+ K GF++FSGLRS A+LPF +K S D F S+++FQT A+GSSGG
Sbjct: 1 MASVTFSVPK--------GFTEFSGLRSSSASLPFGKKLSSDEFVSIVSFQTSAMGSSGG 52
Query: 1246 YKKGVVEAKLKVAINGFGRIGRNFLRCWHGRKDSPLDVIAINDTGGVKQASHLLKYDSTL 1067
Y+KGV EAKLKVAINGFGRIGRNFLRCWHGRKDSPLD+IAINDTGGVKQASHLLKYDSTL
Sbjct: 53 YRKGVTEAKLKVAINGFGRIGRNFLRCWHGRKDSPLDIIAINDTGGVKQASHLLKYDSTL 112
Query: 1066 GIFGADVKPVGTDGISVDGKVIKVVSDRNPANLPWGELGIDLVIEGTGVFVDREGAGKHI 887
GIF ADVKP G ISVDGK+I+VVS+RNP+ LPW ELGID+VIEGTGVFVDREGAGKHI
Sbjct: 113 GIFDADVKPSGETAISVDGKIIQVVSNRNPSLLPWKELGIDIVIEGTGVFVDREGAGKHI 172
Query: 886 QAGAKKVLITAPGKGDIPTYVVGVNADAYSHDDDIISNASCTTNCLAPFVKVLDQKFGII 707
+AGAKKV+ITAPGKGDIPTYVVGVNADAYSHD+ IISNASCTTNCLAPFVKVLDQKFGII
Sbjct: 173 EAGAKKVIITAPGKGDIPTYVVGVNADAYSHDEPIISNASCTTNCLAPFVKVLDQKFGII 232
Query: 706 KGTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPTLKGKLNGIA 527
KGTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLP LKGKLNGIA
Sbjct: 233 KGTMTTTHSYTGDQRLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPNLKGKLNGIA 292
Query: 526 LRVPTPNVSVVDLVVQVTKKTFAEEVNAAFRESADNELNGILSVCDEPLVSVDFRCTDVS 347
LRVPTPNVSVVDLVVQV+KKTFAEEVNAAFR+SA+ EL GIL VCDEPLVSVDFRC+D S
Sbjct: 293 LRVPTPNVSVVDLVVQVSKKTFAEEVNAAFRDSAEKELKGILDVCDEPLVSVDFRCSDFS 352
Query: 346 STVDSSLTMVMGDDMVKVIAWYDNEWGYSQRVVDLADIVANNWK 215
+T+DSSLTMVMGDDMVKVIAWYDNEWGYSQRVVDLADIVANNWK
Sbjct: 353 TTIDSSLTMVMGDDMVKVIAWYDNEWGYSQRVVDLADIVANNWK 396
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,308,991,148
Number of Sequences: 1393205
Number of extensions: 32675532
Number of successful extensions: 113662
Number of sequences better than 10.0: 1931
Number of HSP's better than 10.0 without gapping: 95458
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 109132
length of database: 448,689,247
effective HSP length: 127
effective length of database: 271,752,212
effective search space used: 96200283048
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
