>KMC005144A_C01 KMC005144A_c01
GTAAAAGATACTCCAATAATTAGTGCATATATATAGAAAGACAAAAAAAATTGCCTTAAT
TAACTAGACTCCAAATGTAATGAAACCTGGAGTGCAATAACATGAGTCCAAATAAGTCTA
CTAAAGCAAAGTGAAAACATAGATACAATTTAGCCTACAAAGTTAATTAACTTAAGCTTA
CCCCCATTCCCACCAAACAGGTCAAATATTATACAACCACTACAATCCACAATCCCCAAA
AAAATCTTCATAGGTGATCATCAGCAAATAACTTAACAACTATTTCAAGCCTCCCTCTCC
CAGAAACTCCCAACCGCCTTCTTGTACTTTACACTAAAGCAAAATCAGCCAGATCCAGGT
TCATCATCATCATGCTCATGAAGGATATATCATATATGTGTATCACTCCACTTCAGTATC
CAGTAGGAAATTCACAGCTACCATACTTAGGCTCCTGCGTGACCACGTAGGACGTGCCCC
CGAAATAGCAGCTACCACCAGCACGTGCCTTCTTCTGATAATAACTGTTGAACGCAAACG
AAGCGTGTGCCACCAACGAGTTAGGATCGTAACACGTGGCACCGGGCTGAATAGGACG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC005144A_C01 KMC005144A_c01
         (598 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_568822.1| glycosyl hydrolase family 17; protein id: At5g5...   106  3e-22
gb|AAM66024.1| beta-1,3-glucanase-like protein [Arabidopsis thal...   106  3e-22
gb|AAL77689.1| AT5g55180/MCO15_13 [Arabidopsis thaliana]              106  3e-22
dbj|BAB17320.1| elicitor inducible beta-1,3-glucanase Nt-SubE76 ...   101  6e-21
ref|NP_178637.1| glycosyl hydrolase family 17; protein id: At2g0...    92  5e-18

>ref|NP_568822.1| glycosyl hydrolase family 17; protein id: At5g55180.1, supported by
           cDNA: 7119., supported by cDNA: gi_18700154 [Arabidopsis
           thaliana]
          Length = 460

 Score =  106 bits (264), Expect = 3e-22
 Identities = 46/61 (75%), Positives = 52/61 (84%)
 Frame = -1

Query: 598 RPIQPGATCYDPNSLVAHASFAFNSYYQKKARAGGSCYFGGTSYVVTQEPKYGSCEFPTG 419
           RPIQPGATCY+P SL AHAS+AFNSYYQK AR  G+C FGG +YVV+Q PKYG CEFPTG
Sbjct: 400 RPIQPGATCYNPESLEAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPPKYGKCEFPTG 459

Query: 418 Y 416
           +
Sbjct: 460 H 460

>gb|AAM66024.1| beta-1,3-glucanase-like protein [Arabidopsis thaliana]
          Length = 460

 Score =  106 bits (264), Expect = 3e-22
 Identities = 46/61 (75%), Positives = 52/61 (84%)
 Frame = -1

Query: 598 RPIQPGATCYDPNSLVAHASFAFNSYYQKKARAGGSCYFGGTSYVVTQEPKYGSCEFPTG 419
           RPIQPGATCY+P SL AHAS+AFNSYYQK AR  G+C FGG +YVV+Q PKYG CEFPTG
Sbjct: 400 RPIQPGATCYNPESLEAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPPKYGKCEFPTG 459

Query: 418 Y 416
           +
Sbjct: 460 H 460

>gb|AAL77689.1| AT5g55180/MCO15_13 [Arabidopsis thaliana]
          Length = 460

 Score =  106 bits (264), Expect = 3e-22
 Identities = 46/61 (75%), Positives = 52/61 (84%)
 Frame = -1

Query: 598 RPIQPGATCYDPNSLVAHASFAFNSYYQKKARAGGSCYFGGTSYVVTQEPKYGSCEFPTG 419
           RPIQPGATCY+P SL AHAS+AFNSYYQK AR  G+C FGG +YVV+Q PKYG CEFPTG
Sbjct: 400 RPIQPGATCYNPESLEAHASYAFNSYYQKNARGVGTCNFGGAAYVVSQPPKYGKCEFPTG 459

Query: 418 Y 416
           +
Sbjct: 460 H 460

>dbj|BAB17320.1| elicitor inducible beta-1,3-glucanase Nt-SubE76 [Nicotiana tabacum]
          Length = 467

 Score =  101 bits (252), Expect = 6e-21
 Identities = 44/61 (72%), Positives = 50/61 (81%)
 Frame = -1

Query: 598 RPIQPGATCYDPNSLVAHASFAFNSYYQKKARAGGSCYFGGTSYVVTQEPKYGSCEFPTG 419
           RPIQ GATCYDP++L AHAS+AFNSYYQK  R   +C F G +YVVTQ PKYGSC+FPTG
Sbjct: 407 RPIQQGATCYDPDTLEAHASYAFNSYYQKNTRGVSTCDFSGAAYVVTQHPKYGSCKFPTG 466

Query: 418 Y 416
           Y
Sbjct: 467 Y 467

>ref|NP_178637.1| glycosyl hydrolase family 17; protein id: At2g05790.1 [Arabidopsis
           thaliana] gi|25316444|pir||E84471 probable
           beta-1,3-glucanase [imported] - Arabidopsis thaliana
           gi|4662638|gb|AAD26909.1| putative beta-1,3-glucanase
           [Arabidopsis thaliana] gi|20197850|gb|AAM15281.1|
           putative beta-1,3-glucanase [Arabidopsis thaliana]
          Length = 473

 Score = 92.0 bits (227), Expect = 5e-18
 Identities = 40/50 (80%), Positives = 44/50 (88%)
 Frame = -1

Query: 598 RPIQPGATCYDPNSLVAHASFAFNSYYQKKARAGGSCYFGGTSYVVTQEP 449
           RPIQPGA CY P++L AHASFAFNSYYQKK RAGGSCYFGG +YVV+Q P
Sbjct: 412 RPIQPGANCYSPDTLEAHASFAFNSYYQKKGRAGGSCYFGGAAYVVSQPP 461

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 518,211,423
Number of Sequences: 1393205
Number of extensions: 11102148
Number of successful extensions: 28437
Number of sequences better than 10.0: 132
Number of HSP's better than 10.0 without gapping: 26266
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28156
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23140425222
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF075e07_f BP032279 1 563 2 MWL001b09_f AV768582 1 545 3 MFB054d08_f BP037922 3 452 4 MWM214g11_f AV768028 35 371 5 SPD093b12_f BP051406 35 220 6 SPDL007d12_f BP052414 35 542 7 MF010g08_f BP028771 35 545 8 MF026g02_f BP029650 35 599 9 MPD019b08_f AV771295 36 557 10 SPDL082e03_f BP057124 37 571 11 MF015f05_f BP029046 38 496 12 MPD045d10_f AV773054 40 461 13 MFB064c12_f BP038632 40 596 14 MPDL058c04_f AV779436 45 616 15 MF077g03_f BP032389 57 455 16 MF021f03_f BP029382 58 418 17 MPD073e08_f AV774790 67 515 18 GNf018b11 BP068656 105 497 19 MWL046b03_f AV769347 106 615 20 MPD095c02_f AV776208 109 557

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