>KMC005121A_C01 KMC005121A_c01
aatgaaatttcatctcaatttatatgaagtgttctgttcactttttatctcgaagtaagt
gctagtgtttgtttgtaccgGTGTCAACAAATATCCAAATATACATGAATGAATCGTTGT
CACTCGAGGATTAGCTATTAATGAATGCATGAGATGGACATACATTTATATACAAGGAAT
TTAGTACACGATAAGTATGCTCAAGACCCTTAATTGGCAGTTCAATTTGTAGTAATACCA
TACAAGATAATGTAGGCCGACCCATCTTAACAAGAAATTACTTTTACTCACATTATTGAT
GGTTTCAAAATCAGATGAATGTTTTCATACTTGTGTTATTGCTAAGTAAAACTTTTGTAG
CCTTATTCCACAACAACCATTATTCTACCTCAATTAGTGTGATCTTATCTCAGTATCTGC
TTTGCTTTGGCATTCGGGAGATTTGGCTTGTTACATTTCTCCTGGGGAGGCATGGTATCA
CCACTAGGTAGATCACAAACAGCAATTCCCTCGCCTGTTAGGTGAGCCGTACATCTCTTC
TCAGCTAACAACTCAGCTTCAGACTTGGCTATTCGCTGTCGGAGGTAGTCATCAATTAGA
ACATCTTTGACAAGTTCATAATCACCATCCCCCTTGGATCGGCATGGCATACTGAAAACA
ATATCTTCAGCTATTCCATAAGGATTTCCACTGCTGTATACACCAGAAGAAAACCAGTCA
CCCTCAGGAGTAGGAGTGACTAAAGATCGCA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC005121A_C01 KMC005121A_c01
         (751 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

sp|Q05145|MDHP_MESCR Malate dehydrogenase [NADP], chloroplast pr...   181  8e-45
sp|P21528|MDHP_PEA Malate dehydrogenase [NADP], chloroplast prec...   177  2e-43
sp|O48902|MDHP_MEDSA Malate dehydrogenase [NADP], chloroplast pr...   175  5e-43
sp|P52426|MDHP_SPIOL Malate dehydrogenase [NADP], chloroplast pr...   172  5e-42
ref|NP_568875.1| NADP-dependent malate dehydrogenase; protein id...   170  2e-41

>sp|Q05145|MDHP_MESCR Malate dehydrogenase [NADP], chloroplast precursor (NADP-MDH)
           gi|421790|pir||S33066 malate dehydrogenase (NADP) (EC
           1.1.1.82) - common ice plant gi|19530|emb|CAA45270.1|
           malate dehydrogenase (NADP+) [Mesembryanthemum
           crystallinum]
          Length = 441

 Score =  181 bits (460), Expect = 8e-45
 Identities = 84/97 (86%), Positives = 91/97 (93%)
 Frame = -3

Query: 749 RSLVTPTPEGDWFSSGVYSSGNPYGIAEDIVFSMPCRSKGDGDYELVKDVLIDDYLRQRI 570
           +SLVTPTPEGDWFSS VY++GNPYGIAED+VFSMPCRSKGDGDYELVKDV+ DDYLRQRI
Sbjct: 344 KSLVTPTPEGDWFSSAVYTNGNPYGIAEDLVFSMPCRSKGDGDYELVKDVVFDDYLRQRI 403

Query: 569 AKSEAELLAEKRCTAHLTGEGIAVCDLPSGDTMPPQE 459
            KSE ELLAEKRCTAHLTGEG+AVCDLP+GDTM P E
Sbjct: 404 KKSEEELLAEKRCTAHLTGEGVAVCDLPAGDTMLPGE 440

>sp|P21528|MDHP_PEA Malate dehydrogenase [NADP], chloroplast precursor (NADP-MDH)
           gi|481222|pir||S38346 malate dehydrogenase (NADP) (EC
           1.1.1.82) precursor, chloroplast - garden pea
           gi|397475|emb|CAA52614.1| malate dehydrogenase (NADP+)
           [Pisum sativum]
          Length = 441

 Score =  177 bits (448), Expect = 2e-43
 Identities = 83/97 (85%), Positives = 91/97 (93%)
 Frame = -3

Query: 749 RSLVTPTPEGDWFSSGVYSSGNPYGIAEDIVFSMPCRSKGDGDYELVKDVLIDDYLRQRI 570
           RSL+TPTPEGDWFSSGVY++GNPYGIAEDIVFSMPCRSKGDGDYELV DV+ DDYLRQ++
Sbjct: 345 RSLITPTPEGDWFSSGVYTNGNPYGIAEDIVFSMPCRSKGDGDYELVNDVIFDDYLRQKL 404

Query: 569 AKSEAELLAEKRCTAHLTGEGIAVCDLPSGDTMPPQE 459
           AK+EAELLAEK+C AHLTGEGIAVCDLP GDTM P E
Sbjct: 405 AKTEAELLAEKKCVAHLTGEGIAVCDLP-GDTMLPGE 440

>sp|O48902|MDHP_MEDSA Malate dehydrogenase [NADP], chloroplast precursor (NADP-MDH)
           gi|2827076|gb|AAB99753.1| malate dehydrogenase precursor
           [Medicago sativa]
          Length = 437

 Score =  175 bits (444), Expect = 5e-43
 Identities = 82/97 (84%), Positives = 91/97 (93%)
 Frame = -3

Query: 749 RSLVTPTPEGDWFSSGVYSSGNPYGIAEDIVFSMPCRSKGDGDYELVKDVLIDDYLRQRI 570
           RSL+ PTPEGDWFS+GVY++GNPYGIAEDIVFSMPCRSKGDGDYELVKDV+ DDYLRQ++
Sbjct: 341 RSLIIPTPEGDWFSTGVYTTGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRQKL 400

Query: 569 AKSEAELLAEKRCTAHLTGEGIAVCDLPSGDTMPPQE 459
           AK+EAELLAEK+C AHLTGEGIAVCDLP GDTM P E
Sbjct: 401 AKTEAELLAEKKCVAHLTGEGIAVCDLP-GDTMLPGE 436

>sp|P52426|MDHP_SPIOL Malate dehydrogenase [NADP], chloroplast precursor (NADP-MDH)
           gi|1076266|pir||S52268 malate dehydrogenase (NADP) (EC
           1.1.1.82) precursor, chloroplast - spinach
           gi|642352|emb|CAA58848.1| malate dehydrogenase (NADP+)
           [Spinacia oleracea]
          Length = 435

 Score =  172 bits (436), Expect = 5e-42
 Identities = 78/95 (82%), Positives = 87/95 (91%)
 Frame = -3

Query: 743 LVTPTPEGDWFSSGVYSSGNPYGIAEDIVFSMPCRSKGDGDYELVKDVLIDDYLRQRIAK 564
           L+TPTP GDWF SGVY++GNPYGIAED+++SMPCRSKGDGDYELVKDV+ DDYLR+RI  
Sbjct: 340 LITPTPPGDWFPSGVYTNGNPYGIAEDLIYSMPCRSKGDGDYELVKDVIFDDYLRKRIKT 399

Query: 563 SEAELLAEKRCTAHLTGEGIAVCDLPSGDTMPPQE 459
           SE ELLAEKRCTAHLTGEGIAVCDLP+GDTM P E
Sbjct: 400 SEEELLAEKRCTAHLTGEGIAVCDLPAGDTMLPGE 434

>ref|NP_568875.1| NADP-dependent malate dehydrogenase; protein id: At5g58330.1,
           supported by cDNA: 25200., supported by cDNA:
           gi_18377750 [Arabidopsis thaliana]
           gi|23297668|gb|AAN13004.1| NADP-dependent malate
           dehydrogenase [Arabidopsis thaliana]
          Length = 443

 Score =  170 bits (431), Expect = 2e-41
 Identities = 82/97 (84%), Positives = 86/97 (88%)
 Frame = -3

Query: 749 RSLVTPTPEGDWFSSGVYSSGNPYGIAEDIVFSMPCRSKGDGDYELVKDVLIDDYLRQRI 570
           +SLVTPTPEGDWFS+GVY+ GNPYGI E +VFSMPCRSKGDGDYELVKDV IDDYLRQRI
Sbjct: 346 KSLVTPTPEGDWFSTGVYTDGNPYGIEEGLVFSMPCRSKGDGDYELVKDVEIDDYLRQRI 405

Query: 569 AKSEAELLAEKRCTAHLTGEGIAVCDLPSGDTMPPQE 459
           AKSEAELLAEKRC AHLTGEGIA CDL   DTM P E
Sbjct: 406 AKSEAELLAEKRCVAHLTGEGIAYCDLGPVDTMLPGE 442

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 613,316,606
Number of Sequences: 1393205
Number of extensions: 13052318
Number of successful extensions: 34172
Number of sequences better than 10.0: 136
Number of HSP's better than 10.0 without gapping: 33373
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 34140
length of database: 448,689,247
effective HSP length: 120
effective length of database: 281,504,647
effective search space used: 36314099463
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPD016a05_f AV771065 1 515 2 MF089e10_f BP032978 103 642 3 MF049b01_f BP030853 118 606 4 SPD021e03_f BP045652 119 256 5 SPDL067c09_f BP056153 150 612 6 MPD029b02_f AV771962 162 660 7 MPD064h09_f AV774299 164 558 8 MF091d04_f BP033065 176 722 9 MF077b11_f BP032362 183 660 10 SPD039a07_f BP047069 183 676 11 MFB086g02_f BP040306 187 711 12 SPD020f01_f BP045587 199 697 13 MF037h07_f BP030266 219 297 14 SPD030d01_f BP046374 269 765 15 MPD091c09_f AV775965 381 759

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