KMC005107A_c01
[Fasta Sequence]
[Nr Search]
[EST assemble image]
Fasta Sequence
>KMC005107A_C01 KMC005107A_c01
GATCTGGATCGCGTTCGGTGCACTCGCGGTGGCTCTGCTCTCCGTAACCGCCTCCGCCGA
CGACGTCGTCGTGCTCTCTGAAGATAACTATCGAGAAGGAAGTGGGTCAGGACAAAGGCG
CTCTCGTCGAGTTCTACGCTCCTGTGTGTGGACACTGCAAGAAGCTGGCTCCGGAATATG
AAAAGCTCGGTGGCAGCATTCAAGAAAGCCAAATCTGTTGTGATGGCCAAGGTGGACTGT
GATGAGCATAAGAGTGTGTGCAGCAAATATGGAGTGTCTGGGTACCCAACTCTTCATTGG
TATCCAAAAGGAGTCTCTGGAACCCAAAAAGTAGTGAAGGGCCACGCACTGCACAAGCAC
TTGCTGAGTTTGTAAATACAGAAGGAGGAACCAATGTAGAGATTGCTACGGCTCCATCCA
GGGTAGTGGTGCTAACAGCGGAAAATTTGAATGAGGTTGTCTTGGATGAAACCAAAGATG
Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005107A_C01 KMC005107A_c01
(480 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|P38661|ERP5_MEDSA PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRE... 139 5e-37
ref|NP_182269.1| putative protein disulfide-isomerase; protein i... 132 3e-34
pir||T03644 probable protein disulfide-isomerase (EC 5.3.4.1) pr... 133 3e-32
dbj|BAB67990.1| putative protein disulfide isomerase [Oryza sati... 133 1e-30
gb|AAK70917.1|AC087551_16 putative disulfide-isomerase precursor... 128 2e-30
>sp|P38661|ERP5_MEDSA PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR
gi|7488740|pir||T09614 probable protein
disulfide-isomerase (EC 5.3.4.1) precursor,
glucose-regulated [similarity] - alfalfa
gi|166380|gb|AAB46930.1| glucose-regulated endoplasmic
reticular protein precursor [Medicago sativa]
Length = 364
Score = 139 bits (349), Expect(2) = 5e-37
Identities = 79/135 (58%), Positives = 88/135 (64%), Gaps = 6/135 (4%)
Frame = +3
Query: 93 EKEVGQDKGALVEFYAPVCGHCKKLAPEYEKLGGSIQESQ------ICCDGQGGL**A*E 254
EKEVG DKGALVEFYAP CGHCKKLAPEYEKL S ++++ + CD +
Sbjct: 40 EKEVGHDKGALVEFYAPWCGHCKKLAPEYEKLPNSFKKAKSVLIAKVDCDEHKSVCSKYG 99
Query: 255 CVQQIWSVWVPNSSLVSKRSLWNPKSSEGPRTAQALAEFVNTEGGTNVEIATAPSRVVVL 434
W P SL PK EGPRTA++LAEFVNTEGGTNV+IATAPS VVVL
Sbjct: 100 VSGYPTIQWFPKGSL-------EPKKFEGPRTAESLAEFVNTEGGTNVKIATAPSHVVVL 152
Query: 435 TAENLNEVVLDETKD 479
T E NEVVLD TKD
Sbjct: 153 TPETFNEVVLDGTKD 167
Score = 47.0 bits (110), Expect(2) = 3e-12
Identities = 19/40 (47%), Positives = 28/40 (69%)
Frame = +1
Query: 193 AAFKKAKSVVMAKVDCDEHKSVCSKYGVSGYPTLHWYPKG 312
A FK VV+A +D D+++ + KY VSG+PTL ++PKG
Sbjct: 192 AVFKSEDDVVIANLDADKYRDLAEKYDVSGFPTLKFFPKG 231
Score = 45.1 bits (105), Expect(2) = 3e-12
Identities = 19/25 (76%), Positives = 20/25 (80%)
Frame = +3
Query: 114 KGALVEFYAPVCGHCKKLAPEYEKL 188
K LVEFYAP CGHCK LAP YEK+
Sbjct: 166 KDVLVEFYAPWCGHCKSLAPIYEKV 190
Score = 36.6 bits (83), Expect(2) = 5e-37
Identities = 19/37 (51%), Positives = 26/37 (69%), Gaps = 2/37 (5%)
Frame = +2
Query: 2 IW--IAFGALAVALLSVTASADDVVVLSEDNYREGSG 106
IW IA + A A+L V+ SADDVVVL+E+N+ + G
Sbjct: 8 IWSRIALASFAFAILFVSVSADDVVVLTEENFEKEVG 44
>ref|NP_182269.1| putative protein disulfide-isomerase; protein id: At2g47470.1,
supported by cDNA: gi_18377788, supported by cDNA:
gi_20259222 [Arabidopsis thaliana]
gi|11132051|sp|O22263|PDA6_ARATH Probable protein
disulfide isomerase A6 precursor (P5)
gi|7488158|pir||T00437 probable protein
disulfide-isomerase (EC 5.3.4.1) precursor - Arabidopsis
thaliana gi|2529680|gb|AAC62863.1| putative protein
disulfide-isomerase [Arabidopsis thaliana]
gi|18377789|gb|AAL67044.1| putative protein
disulfide-isomerase [Arabidopsis thaliana]
gi|20259223|gb|AAM14327.1| putative protein
disulfide-isomerase [Arabidopsis thaliana]
gi|24417274|gb|AAN60247.1| unknown [Arabidopsis
thaliana]
Length = 361
Score = 132 bits (332), Expect(2) = 3e-34
Identities = 73/142 (51%), Positives = 91/142 (63%), Gaps = 11/142 (7%)
Frame = +3
Query: 87 TIEKEVGQDKGALVEFYAPVCGHCKKLAPEYEKLGGSIQE------SQICCDGQGGL**A 248
+ EKEVG+DKGALVEFYAP CGHCKKLAPEYEKLG S ++ +++ CD Q +
Sbjct: 32 SFEKEVGKDKGALVEFYAPWCGHCKKLAPEYEKLGASFKKAKSVLIAKVDCDEQKSV--- 88
Query: 249 *ECVQQIWS-----VWVPNSSLVSKRSLWNPKSSEGPRTAQALAEFVNTEGGTNVEIATA 413
C + S W P SL P+ EGPR A+ALAE+VN EGGTNV++A
Sbjct: 89 --CTKYGVSGYPTIQWFPKGSL-------EPQKYEGPRNAEALAEYVNKEGGTNVKLAAV 139
Query: 414 PSRVVVLTAENLNEVVLDETKD 479
P VVVLT +N +E+VLD+ KD
Sbjct: 140 PQNVVVLTPDNFDEIVLDQNKD 161
Score = 77.8 bits (190), Expect = 6e-14
Identities = 34/46 (73%), Positives = 40/46 (86%)
Frame = +1
Query: 193 AAFKKAKSVVMAKVDCDEHKSVCSKYGVSGYPTLHWYPKGVSGTQK 330
A+FKKAKSV++AKVDCDE KSVC+KYGVSGYPT+ W+PKG QK
Sbjct: 67 ASFKKAKSVLIAKVDCDEQKSVCTKYGVSGYPTIQWFPKGSLEPQK 112
Score = 48.9 bits (115), Expect(2) = 9e-14
Identities = 20/37 (54%), Positives = 29/37 (78%)
Frame = +1
Query: 199 FKKAKSVVMAKVDCDEHKSVCSKYGVSGYPTLHWYPK 309
FK+ + VV+A +D D HK++ KYGVSG+PTL ++PK
Sbjct: 188 FKQEEGVVIANLDADAHKALGEKYGVSGFPTLKFFPK 224
Score = 48.5 bits (114), Expect(2) = 9e-14
Identities = 20/35 (57%), Positives = 25/35 (71%)
Frame = +3
Query: 108 QDKGALVEFYAPVCGHCKKLAPEYEKLGGSIQESQ 212
Q+K LVEFYAP CGHCK LAP YEK+ ++ +
Sbjct: 158 QNKDVLVEFYAPWCGHCKSLAPTYEKVATVFKQEE 192
Score = 33.9 bits (76), Expect(2) = 3e-34
Identities = 18/35 (51%), Positives = 24/35 (68%)
Frame = +2
Query: 2 IWIAFGALAVALLSVTASADDVVVLSEDNYREGSG 106
IW F LA LL V+A ADDVVVL++D++ + G
Sbjct: 6 IWFGFALLA--LLLVSAVADDVVVLTDDSFEKEVG 38
>pir||T03644 probable protein disulfide-isomerase (EC 5.3.4.1) precursor -
common tobacco gi|1848212|emb|CAA72092.1| protein
disulfide-isomerase precursor [Nicotiana tabacum]
Length = 359
Score = 133 bits (335), Expect(2) = 3e-32
Identities = 76/140 (54%), Positives = 94/140 (66%), Gaps = 11/140 (7%)
Frame = +3
Query: 93 EKEVGQDKGALVEFYAPVCGHCKKLAPEYEKLGGSIQESQ------ICCDGQGGL**A*E 254
EKE+GQD+ ALVEFYAP CGHCKKLAPEYEKLG S ++++ + CD +
Sbjct: 35 EKEIGQDRAALVEFYAPWCGHCKKLAPEYEKLGASFRKAKSILIGKVDCDEHKSV----- 89
Query: 255 C----VQQIWSV-WVPNSSLVSKRSLWNPKSSEGPRTAQALAEFVNTEGGTNVEIATAPS 419
C VQ ++ W P SL PK EG RTA+ALAEFVN+EGGTNV+IA+ PS
Sbjct: 90 CSKYGVQGYPTIQWFPKGSL-------EPKKYEGGRTAEALAEFVNSEGGTNVKIASTPS 142
Query: 420 RVVVLTAENLNEVVLDETKD 479
VVVL+ +N +E+VLDETKD
Sbjct: 143 SVVVLSPDNFDEIVLDETKD 162
Score = 50.8 bits (120), Expect(3) = 1e-14
Identities = 20/39 (51%), Positives = 31/39 (79%)
Frame = +1
Query: 196 AFKKAKSVVMAKVDCDEHKSVCSKYGVSGYPTLHWYPKG 312
+FK+ + VV+A +D D+H+ + KYGVSG+PTL ++PKG
Sbjct: 188 SFKQEEDVVIANLDADKHRDLGEKYGVSGFPTLKFFPKG 226
Score = 48.1 bits (113), Expect(3) = 1e-14
Identities = 20/35 (57%), Positives = 25/35 (71%)
Frame = +3
Query: 108 QDKGALVEFYAPVCGHCKKLAPEYEKLGGSIQESQ 212
+ K LVEFYAP CGHCK LAP YEK+ S ++ +
Sbjct: 159 ETKDVLVEFYAPWCGHCKSLAPIYEKVATSFKQEE 193
Score = 25.8 bits (55), Expect(2) = 3e-32
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = +2
Query: 20 ALAVALLSVTASADDVVVLSEDNYREGSG 106
A+ L +A A+DVVVL+E+N+ + G
Sbjct: 11 AILALFLFSSALAEDVVVLTEENFEKEIG 39
Score = 20.8 bits (42), Expect(3) = 1e-14
Identities = 8/20 (40%), Positives = 13/20 (65%)
Frame = +2
Query: 38 LSVTASADDVVVLSEDNYRE 97
+ + ++ VVVLS DN+ E
Sbjct: 135 VKIASTPSSVVVLSPDNFDE 154
>dbj|BAB67990.1| putative protein disulfide isomerase [Oryza sativa (japonica
cultivar-group)]
Length = 371
Score = 133 bits (334), Expect = 1e-30
Identities = 74/137 (54%), Positives = 88/137 (64%), Gaps = 6/137 (4%)
Frame = +3
Query: 87 TIEKEVGQDKGALVEFYAPVCGHCKKLAPEYEKLGGSIQE------SQICCDGQGGL**A 248
T EKEVGQD+GALVEFYAP CGHCKKLAPEYEKLG S ++ +++ CD +
Sbjct: 44 TFEKEVGQDRGALVEFYAPWCGHCKKLAPEYEKLGASFKKAKSVFIAKVDCDEHKSVCSK 103
Query: 249 *ECVQQIWSVWVPNSSLVSKRSLWNPKSSEGPRTAQALAEFVNTEGGTNVEIATAPSRVV 428
W P SL PK EG R+A+ALAEFVNTEGGTNV++AT PS VV
Sbjct: 104 YGVSGYPTIQWFPKGSL-------EPKKYEGQRSAEALAEFVNTEGGTNVKLATIPSSVV 156
Query: 429 VLTAENLNEVVLDETKD 479
VL +N + +VLDE KD
Sbjct: 157 VLGPDNFDSIVLDENKD 173
Score = 80.1 bits (196), Expect = 1e-14
Identities = 34/40 (85%), Positives = 38/40 (95%)
Frame = +1
Query: 193 AAFKKAKSVVMAKVDCDEHKSVCSKYGVSGYPTLHWYPKG 312
A+FKKAKSV +AKVDCDEHKSVCSKYGVSGYPT+ W+PKG
Sbjct: 79 ASFKKAKSVFIAKVDCDEHKSVCSKYGVSGYPTIQWFPKG 118
Score = 51.2 bits (121), Expect(2) = 7e-14
Identities = 21/40 (52%), Positives = 29/40 (72%)
Frame = +1
Query: 193 AAFKKAKSVVMAKVDCDEHKSVCSKYGVSGYPTLHWYPKG 312
+ +K VV+A +D D+HK + KYGVSGYPTL ++PKG
Sbjct: 198 SVYKLDDGVVIANLDADKHKDLAEKYGVSGYPTLKFFPKG 237
Score = 46.6 bits (109), Expect(2) = 7e-14
Identities = 20/27 (74%), Positives = 22/27 (81%)
Frame = +3
Query: 108 QDKGALVEFYAPVCGHCKKLAPEYEKL 188
++K LVEFYAP CGHCK LAP YEKL
Sbjct: 170 ENKDILVEFYAPWCGHCKHLAPIYEKL 196
>gb|AAK70917.1|AC087551_16 putative disulfide-isomerase precursor [Oryza sativa]
Length = 366
Score = 128 bits (321), Expect(2) = 2e-30
Identities = 73/137 (53%), Positives = 85/137 (61%), Gaps = 6/137 (4%)
Frame = +3
Query: 87 TIEKEVGQDKGALVEFYAPVCGHCKKLAPEYEKLGGSIQE------SQICCDGQGGL**A 248
T EKEVGQD+ ALVEFYAP CGHCKKLAPEYEKLG S ++ +++ CD +
Sbjct: 39 TFEKEVGQDRAALVEFYAPWCGHCKKLAPEYEKLGASFKKAKSVLIAKVDCDEHKSVCSK 98
Query: 249 *ECVQQIWSVWVPNSSLVSKRSLWNPKSSEGPRTAQALAEFVNTEGGTNVEIATAPSRVV 428
W P SL PK EG RTA+ALAE+VN+E TNV+IA PS VV
Sbjct: 99 YGVSGYPTIQWFPKGSL-------EPKKYEGQRTAEALAEYVNSEAATNVKIAAVPSSVV 151
Query: 429 VLTAENLNEVVLDETKD 479
VLT E + VVLDETKD
Sbjct: 152 VLTPETFDSVVLDETKD 168
Score = 80.9 bits (198), Expect = 7e-15
Identities = 34/40 (85%), Positives = 39/40 (97%)
Frame = +1
Query: 193 AAFKKAKSVVMAKVDCDEHKSVCSKYGVSGYPTLHWYPKG 312
A+FKKAKSV++AKVDCDEHKSVCSKYGVSGYPT+ W+PKG
Sbjct: 74 ASFKKAKSVLIAKVDCDEHKSVCSKYGVSGYPTIQWFPKG 113
Score = 49.7 bits (117), Expect(2) = 1e-13
Identities = 19/40 (47%), Positives = 31/40 (77%)
Frame = +1
Query: 193 AAFKKAKSVVMAKVDCDEHKSVCSKYGVSGYPTLHWYPKG 312
+ +K+ + VV+A +D D+H ++ KYGVSG+PTL ++PKG
Sbjct: 193 SVYKQDEGVVIANLDADKHTALAEKYGVSGFPTLKFFPKG 232
Score = 47.0 bits (110), Expect(2) = 1e-13
Identities = 20/35 (57%), Positives = 24/35 (68%)
Frame = +3
Query: 108 QDKGALVEFYAPVCGHCKKLAPEYEKLGGSIQESQ 212
+ K LVEFYAP CGHCK LAP YEKL ++ +
Sbjct: 165 ETKDVLVEFYAPWCGHCKHLAPIYEKLASVYKQDE 199
Score = 25.0 bits (53), Expect(2) = 2e-30
Identities = 12/28 (42%), Positives = 17/28 (59%)
Frame = +2
Query: 23 LAVALLSVTASADDVVVLSEDNYREGSG 106
+A A TASADDV+ L+E + + G
Sbjct: 18 VAAAAAVSTASADDVLALTESTFEKEVG 45
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 465,716,699
Number of Sequences: 1393205
Number of extensions: 10703098
Number of successful extensions: 42297
Number of sequences better than 10.0: 626
Number of HSP's better than 10.0 without gapping: 37496
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 41627
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 13397825772
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
EST assemble image
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clone |
accession |
position |
| 1 |
MPD085g04_f |
AV775597 |
1 |
434 |
| 2 |
MPD014d07_f |
AV770955 |
1 |
480 |
|
Lotus japonicus
Kazusa DNA Research Institute