Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005074A_C01 KMC005074A_c01
(975 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_189227.1| putative mitotic checkpoint protein; protein id... 365 e-100
emb|CAD41403.1| OJ000223_09.15 [Oryza sativa (japonica cultivar-... 350 1e-95
sp|Q9XFH3|MAD2_MAIZE Mitotic spindle checkpoint protein MAD2 gi|... 350 2e-95
emb|CAD79699.1| putative mitotic spindle checkpoint protein [Ory... 340 2e-92
gb|AAO51442.1| similar to Homo sapiens (Human). MAD2-like protei... 217 2e-55
>ref|NP_189227.1| putative mitotic checkpoint protein; protein id: At3g25980.1
[Arabidopsis thaliana] gi|12230292|sp|Q9LU93|MAD2_ARATH
Mitotic spindle checkpoint protein MAD2
gi|9279603|dbj|BAB01061.1| cell cycle checkpoint protein
MAD2-like [Arabidopsis thaliana]
Length = 209
Score = 365 bits (937), Expect = e-100
Identities = 182/208 (87%), Positives = 195/208 (93%), Gaps = 1/208 (0%)
Frame = -2
Query: 956 MAARTVT-KDIITLRGSASIVSEFFGYAANSILYNRGVYPEESFVKVKKYGLPMLLTEDE 780
MA++T KDIITL GSA+IVSEFF YAANSILYNR VYPEESFVKVKKYGLPMLL EDE
Sbjct: 1 MASKTAAAKDIITLHGSAAIVSEFFCYAANSILYNRAVYPEESFVKVKKYGLPMLLIEDE 60
Query: 779 GVKSFISNLTAQLSEWLESGKLQRVVLVIMSKATGEVLERWNFSIETDSEVVEKSVSREK 600
VKSF+SNLT+Q+SEWLE+GKLQRVVLVIMSKATGEVLERWNF IETD+EVV+K VSREK
Sbjct: 61 SVKSFMSNLTSQISEWLEAGKLQRVVLVIMSKATGEVLERWNFRIETDNEVVDKGVSREK 120
Query: 599 SDKEIMREIQAIMRQIASSITYLPCLDESCVLDVLAYTDTDVAVPFTWIESDPKLIENPQ 420
SDKEIMREIQAIMRQ+ASS+TYLPCLDE+CV DVLAYTDTDVAVPFTWIESDPKLI NPQ
Sbjct: 121 SDKEIMREIQAIMRQVASSVTYLPCLDETCVFDVLAYTDTDVAVPFTWIESDPKLIANPQ 180
Query: 419 MVKLHSFDTKIHKVDTLVSYKNDEWDEQ 336
MVKLH FDTKIHKVDTLVSYKNDEWDE+
Sbjct: 181 MVKLHGFDTKIHKVDTLVSYKNDEWDEE 208
>emb|CAD41403.1| OJ000223_09.15 [Oryza sativa (japonica cultivar-group)]
Length = 207
Score = 350 bits (899), Expect = 1e-95
Identities = 172/207 (83%), Positives = 189/207 (91%)
Frame = -2
Query: 956 MAARTVTKDIITLRGSASIVSEFFGYAANSILYNRGVYPEESFVKVKKYGLPMLLTEDEG 777
MA+RT +KDIITLRGSA+IVSEFFG + + ILYNRGVYPEESF KVKKYGL MLLT+DEG
Sbjct: 1 MASRTASKDIITLRGSAAIVSEFFGRSRSPILYNRGVYPEESFTKVKKYGLTMLLTQDEG 60
Query: 776 VKSFISNLTAQLSEWLESGKLQRVVLVIMSKATGEVLERWNFSIETDSEVVEKSVSREKS 597
VK+FI+NL QLSEWLE+GKLQR+VLVIMSKAT EVLERWNFSI+TD EVV+K V +EKS
Sbjct: 61 VKTFIANLNTQLSEWLEAGKLQRIVLVIMSKATSEVLERWNFSIQTDPEVVDKGVIKEKS 120
Query: 596 DKEIMREIQAIMRQIASSITYLPCLDESCVLDVLAYTDTDVAVPFTWIESDPKLIENPQM 417
DKEIMREIQAIMRQ+AS ITYLPCLDE C+ DVLAYTD DVAVPFTW+ESD KLIENPQM
Sbjct: 121 DKEIMREIQAIMRQVASCITYLPCLDEPCIFDVLAYTDMDVAVPFTWVESDAKLIENPQM 180
Query: 416 VKLHSFDTKIHKVDTLVSYKNDEWDEQ 336
VKLHSFDTKIHKVDTLVSYK DEWDE+
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKVDEWDEE 207
>sp|Q9XFH3|MAD2_MAIZE Mitotic spindle checkpoint protein MAD2
gi|4836787|gb|AAD30555.1|AF143681_1 cell cycle
checkpoint protein MAD2 homolog [Zea mays]
Length = 208
Score = 350 bits (898), Expect = 2e-95
Identities = 173/207 (83%), Positives = 190/207 (91%)
Frame = -2
Query: 956 MAARTVTKDIITLRGSASIVSEFFGYAANSILYNRGVYPEESFVKVKKYGLPMLLTEDEG 777
MA+R+ +KDIITLRGSA+IVSEFFGYAANSILYNR VYPEESF KVKKYGL MLLT+DEG
Sbjct: 1 MASRSASKDIITLRGSAAIVSEFFGYAANSILYNRAVYPEESFSKVKKYGLTMLLTQDEG 60
Query: 776 VKSFISNLTAQLSEWLESGKLQRVVLVIMSKATGEVLERWNFSIETDSEVVEKSVSREKS 597
VK+FI++LT+QLSEWLE+GKLQR+VLVIMSKAT EVLERWNF+I TD+EVVEK +EKS
Sbjct: 61 VKNFIASLTSQLSEWLEAGKLQRIVLVIMSKATSEVLERWNFNIVTDAEVVEKGAIKEKS 120
Query: 596 DKEIMREIQAIMRQIASSITYLPCLDESCVLDVLAYTDTDVAVPFTWIESDPKLIENPQM 417
DKEIMREIQAIMRQIAS ITYLPCLDE CV DVLAYTDTDV P TW+ESD KLI+NPQM
Sbjct: 121 DKEIMREIQAIMRQIASCITYLPCLDEPCVFDVLAYTDTDVDAPGTWVESDAKLIDNPQM 180
Query: 416 VKLHSFDTKIHKVDTLVSYKNDEWDEQ 336
VKLHSFDTKIHKVDTLVSYK DEWDE+
Sbjct: 181 VKLHSFDTKIHKVDTLVSYKKDEWDEE 207
>emb|CAD79699.1| putative mitotic spindle checkpoint protein [Oryza sativa (indica
cultivar-group)]
Length = 221
Score = 340 bits (872), Expect = 2e-92
Identities = 173/221 (78%), Positives = 188/221 (84%), Gaps = 14/221 (6%)
Frame = -2
Query: 956 MAARTVTKDIITLRGSASIVSEFFGY--------------AANSILYNRGVYPEESFVKV 819
MA+RT +KDIITLRGSA+IVSEFF A SILYNRGVYPEESF KV
Sbjct: 1 MASRTASKDIITLRGSAAIVSEFFDSGLISDPGCFVFLVCAWRSILYNRGVYPEESFTKV 60
Query: 818 KKYGLPMLLTEDEGVKSFISNLTAQLSEWLESGKLQRVVLVIMSKATGEVLERWNFSIET 639
KKYGL MLLT+DEGVK+FI+NL QLSEWLE+GKLQR+VLVIMSKAT EVLERWNFSI+T
Sbjct: 61 KKYGLTMLLTQDEGVKTFIANLNTQLSEWLEAGKLQRIVLVIMSKATSEVLERWNFSIQT 120
Query: 638 DSEVVEKSVSREKSDKEIMREIQAIMRQIASSITYLPCLDESCVLDVLAYTDTDVAVPFT 459
D EVV+K V +EKSDKEIMREIQAIMRQ+AS ITYLPCLDE C+ DVLAYTD DVAVPFT
Sbjct: 121 DPEVVDKGVIKEKSDKEIMREIQAIMRQVASCITYLPCLDEPCIFDVLAYTDMDVAVPFT 180
Query: 458 WIESDPKLIENPQMVKLHSFDTKIHKVDTLVSYKNDEWDEQ 336
W+ESD KLIENPQMVKLHSFDTKIHKVDTLVSYK DEWDE+
Sbjct: 181 WVESDAKLIENPQMVKLHSFDTKIHKVDTLVSYKVDEWDEE 221
>gb|AAO51442.1| similar to Homo sapiens (Human). MAD2-like protein 1 [Dictyostelium
discoideum]
Length = 203
Score = 217 bits (553), Expect = 2e-55
Identities = 108/200 (54%), Positives = 141/200 (70%)
Frame = -2
Query: 956 MAARTVTKDIITLRGSASIVSEFFGYAANSILYNRGVYPEESFVKVKKYGLPMLLTEDEG 777
M A +K I+L+GS IV+EFF Y+ N+IL+ RG+YP ESF +V KYGLP+L+T D+
Sbjct: 1 MQAAVASKTNISLKGSTEIVTEFFSYSINTILFQRGLYPPESFTRVAKYGLPILVTNDQS 60
Query: 776 VKSFISNLTAQLSEWLESGKLQRVVLVIMSKATGEVLERWNFSIETDSEVVEKSVSREKS 597
+K ++ N+ QLSEWL SG +Q++VLVI T EVLERW F + TD E R+K
Sbjct: 61 LKDYLDNVLKQLSEWLLSGDVQKLVLVITDIVTKEVLERWVFDVTTDIP-KEGEAPRQKP 119
Query: 596 DKEIMREIQAIMRQIASSITYLPCLDESCVLDVLAYTDTDVAVPFTWIESDPKLIENPQM 417
+KEIM EIQAI+RQI +S+T+LP L +C D+L YT D+AVP W ESDPK I N Q
Sbjct: 120 EKEIMNEIQAIIRQITASVTFLPLLPNACTFDLLVYTSKDLAVPQKWEESDPKFITNSQQ 179
Query: 416 VKLHSFDTKIHKVDTLVSYK 357
VKL SF T IHKV+++V+YK
Sbjct: 180 VKLRSFTTTIHKVESMVAYK 199
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 785,534,689
Number of Sequences: 1393205
Number of extensions: 16750914
Number of successful extensions: 49877
Number of sequences better than 10.0: 55
Number of HSP's better than 10.0 without gapping: 46573
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 49699
length of database: 448,689,247
effective HSP length: 123
effective length of database: 277,325,032
effective search space used: 55742331432
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
