Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005064A_C01 KMC005064A_c01
(651 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
prf||1803516A glycolate oxidase 123 9e-55
ref|NP_188059.1| glycolate oxidase; protein id: At3g14415.1 [Ara... 126 4e-53
gb|AAL38298.1| glycolate oxidase [Arabidopsis thaliana] gi|20148... 127 4e-53
ref|NP_188060.1| glycolate oxidase, putative; protein id: At3g14... 127 9e-53
gb|AAL16258.1|AF428328_1 AT3g14420/MOA2_2 [Arabidopsis thaliana] 127 9e-53
>prf||1803516A glycolate oxidase
Length = 371
Score = 123 bits (308), Expect(2) = 9e-55
Identities = 62/67 (92%), Positives = 64/67 (94%)
Frame = -3
Query: 649 SGAAGIIVSNHGARQLDYVPATISALEEVVKAAQGRIPVFLDGGVRRGTDVFKALALGAS 470
SGAAGIIVSNHGARQLDYV ATISALEEVVKAAQGR+PVFLDGGVRRGTDVFKALALGA
Sbjct: 244 SGAAGIIVSNHGARQLDYVLATISALEEVVKAAQGRVPVFLDGGVRRGTDVFKALALGAR 303
Query: 469 GIFIGTP 449
G+FIG P
Sbjct: 304 GVFIGRP 310
Score = 112 bits (281), Expect(2) = 9e-55
Identities = 52/65 (80%), Positives = 59/65 (90%)
Frame = -1
Query: 459 LGRPVVYALAAEGETGVRKVLQMLRDEFELTMALSGCRSLKEITRDHIATDWDAPRVQPR 280
+GRPVV++LAAEGE GV+KVLQMLRDEFE+TM LSGCRSLKEITR+ I DWD PR+QPR
Sbjct: 307 IGRPVVFSLAAEGEVGVKKVLQMLRDEFEMTMTLSGCRSLKEITREMIVADWDTPRIQPR 366
Query: 279 ALPRL 265
ALPRL
Sbjct: 367 ALPRL 371
>ref|NP_188059.1| glycolate oxidase; protein id: At3g14415.1 [Arabidopsis thaliana]
gi|13124263|sp|Q9LRS0|GOX1_ARATH Probable
(S)-2-hydroxy-acid oxidase, peroxisomal 1 (Glycolate
oxidase 1) (GOX 1) (Short chain alpha-hydroxy acid
oxidase 1) gi|16604394|gb|AAL24203.1| AT3g14420/MOA2_2
[Arabidopsis thaliana] gi|22531128|gb|AAM97068.1|
glycolate oxidase [Arabidopsis thaliana]
gi|25083945|gb|AAN72140.1| glycolate oxidase
[Arabidopsis thaliana]
Length = 367
Score = 126 bits (317), Expect(2) = 4e-53
Identities = 63/67 (94%), Positives = 65/67 (96%)
Frame = -3
Query: 649 SGAAGIIVSNHGARQLDYVPATISALEEVVKAAQGRIPVFLDGGVRRGTDVFKALALGAS 470
+GAAGIIVSNHGARQLDYVPATISALEEVVKA QGR+PVFLDGGVRRGTDVFKALALGAS
Sbjct: 244 AGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRVPVFLDGGVRRGTDVFKALALGAS 303
Query: 469 GIFIGTP 449
GIFIG P
Sbjct: 304 GIFIGRP 310
Score = 103 bits (258), Expect(2) = 4e-53
Identities = 49/60 (81%), Positives = 54/60 (89%)
Frame = -1
Query: 459 LGRPVVYALAAEGETGVRKVLQMLRDEFELTMALSGCRSLKEITRDHIATDWDAPRVQPR 280
+GRPVV+ALAAEGE GV+KVLQMLRDEFELTMALSGCRSL EITR+HI T+WD PR PR
Sbjct: 307 IGRPVVFALAAEGEAGVKKVLQMLRDEFELTMALSGCRSLSEITRNHIVTEWDTPRHLPR 366
>gb|AAL38298.1| glycolate oxidase [Arabidopsis thaliana] gi|20148475|gb|AAM10128.1|
glycolate oxidase [Arabidopsis thaliana]
Length = 177
Score = 127 bits (318), Expect(2) = 4e-53
Identities = 64/67 (95%), Positives = 65/67 (96%)
Frame = -3
Query: 649 SGAAGIIVSNHGARQLDYVPATISALEEVVKAAQGRIPVFLDGGVRRGTDVFKALALGAS 470
+GAAGIIVSNHGARQLDYVPATISALEEVVKA QGRIPVFLDGGVRRGTDVFKALALGAS
Sbjct: 54 AGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALGAS 113
Query: 469 GIFIGTP 449
GIFIG P
Sbjct: 114 GIFIGRP 120
Score = 103 bits (257), Expect(2) = 4e-53
Identities = 48/60 (80%), Positives = 55/60 (91%)
Frame = -1
Query: 459 LGRPVVYALAAEGETGVRKVLQMLRDEFELTMALSGCRSLKEITRDHIATDWDAPRVQPR 280
+GRPVV++LAAEGE GVRKVLQMLRDEFELTMALSGCRSLKEI+R+HI T+WD PR+ R
Sbjct: 117 IGRPVVFSLAAEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEWDTPRLSAR 176
>ref|NP_188060.1| glycolate oxidase, putative; protein id: At3g14420.1, supported by
cDNA: gi_15450740, supported by cDNA: gi_16226422,
supported by cDNA: gi_16226771, supported by cDNA:
gi_16604393, supported by cDNA: gi_18491118 [Arabidopsis
thaliana] gi|13124262|sp|Q9LRR9|GOX2_ARATH Probable
(S)-2-hydroxy-acid oxidase, peroxisomal 2 (Glycolate
oxidase 2) (GOX 2) (Short chain alpha-hydroxy acid
oxidase 2) gi|11994212|dbj|BAB01334.1| glycolate oxidase
[Arabidopsis thaliana] gi|15450741|gb|AAK96642.1|
AT3g14420/MOA2_2 [Arabidopsis thaliana]
gi|16226423|gb|AAL16164.1|AF428396_1 AT3g14420/MOA2_2
[Arabidopsis thaliana] gi|18491119|gb|AAL69528.1|
AT3g14420/MOA2_2 [Arabidopsis thaliana]
Length = 367
Score = 127 bits (318), Expect(2) = 9e-53
Identities = 64/67 (95%), Positives = 65/67 (96%)
Frame = -3
Query: 649 SGAAGIIVSNHGARQLDYVPATISALEEVVKAAQGRIPVFLDGGVRRGTDVFKALALGAS 470
+GAAGIIVSNHGARQLDYVPATISALEEVVKA QGRIPVFLDGGVRRGTDVFKALALGAS
Sbjct: 244 AGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALGAS 303
Query: 469 GIFIGTP 449
GIFIG P
Sbjct: 304 GIFIGRP 310
Score = 102 bits (254), Expect(2) = 9e-53
Identities = 48/60 (80%), Positives = 54/60 (90%)
Frame = -1
Query: 459 LGRPVVYALAAEGETGVRKVLQMLRDEFELTMALSGCRSLKEITRDHIATDWDAPRVQPR 280
+GRPVV++LAAEGE GVRKVLQMLRDEFELTMALSGCRSLKEI+R+HI T+WD PR R
Sbjct: 307 IGRPVVFSLAAEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEWDTPRPSAR 366
>gb|AAL16258.1|AF428328_1 AT3g14420/MOA2_2 [Arabidopsis thaliana]
Length = 367
Score = 127 bits (318), Expect(2) = 9e-53
Identities = 64/67 (95%), Positives = 65/67 (96%)
Frame = -3
Query: 649 SGAAGIIVSNHGARQLDYVPATISALEEVVKAAQGRIPVFLDGGVRRGTDVFKALALGAS 470
+GAAGIIVSNHGARQLDYVPATISALEEVVKA QGRIPVFLDGGVRRGTDVFKALALGAS
Sbjct: 244 AGAAGIIVSNHGARQLDYVPATISALEEVVKATQGRIPVFLDGGVRRGTDVFKALALGAS 303
Query: 469 GIFIGTP 449
GIFIG P
Sbjct: 304 GIFIGRP 310
Score = 102 bits (254), Expect(2) = 9e-53
Identities = 48/60 (80%), Positives = 54/60 (90%)
Frame = -1
Query: 459 LGRPVVYALAAEGETGVRKVLQMLRDEFELTMALSGCRSLKEITRDHIATDWDAPRVQPR 280
+GRPVV++LAAEGE GVRKVLQMLRDEFELTMALSGCRSLKEI+R+HI T+WD PR R
Sbjct: 307 IGRPVVFSLAAEGEAGVRKVLQMLRDEFELTMALSGCRSLKEISRNHITTEWDTPRPSAR 366
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 585,839,242
Number of Sequences: 1393205
Number of extensions: 13159863
Number of successful extensions: 46521
Number of sequences better than 10.0: 536
Number of HSP's better than 10.0 without gapping: 43778
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 46386
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 27860523586
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
