>KMC005061A_C01 KMC005061A_c01
tcgggccccccttcagctatggagccttattttctctacattattttctctcagtatgat
ggtgatgttagcctatgctgCCTTACCTCCTCAACTTGACTGGAAAATGATGCTTCCAAC
TACGCCGGTTCCGAAAGCAATCACAGAGCAACTAAGCCTTGGAGATATTGTGAGCAGAGG
GAAGAGTGTTAATGATGCCGAAACTCAATCACAAAACATGTTCCATGGATTCTAAGGCAG
ATTCCACCACAATGAGATAGGATCATGTTTTGGTTGTAATGTGCACTCAAGCTTCTTGGT
CTATAGACACAATATCTTGTATGGTGTATAAACATTGTGCGCTAAAGCAGATCCAAGCTT
CATGCATGACACTAAATCTGTTATGGAGTATAAGAAGCTGGTGCTAGTCTTCACAAGTCG
CTGCACGCAAAAAACAAAAGCCGGTGTTTTTTTCTTCGAAGAAAACCTTCATCCAGGCAC
CAAATTGGATTTGCAATTGAGTAAGAGAAAACCTGCAGTCCCAATTTTGCCTCGCACAAT
CGCAGACCAGATACCATTCTCACTGGAAAAGTTGAAAGAAAGTATTGGAGATACTTGTAG
TGAAACCCGACTCAAAGAAGACTGAGATTGTAGAGAAAACAATGGGTGGGTGTGAATTGC
CTTCAATGGAAGGAGAAGAACAAATGTGTGCTACTTCATTGGAATCCTTGGTGGATTTCA
TCGCTTCCAAGCTTGGGAAAAATGTGCTTGCTATCTCAACAGAAGTGGAATTGGAAAAGG
AAACTCAATCACAGAATTTGTTAGTGAAAGATGGAGTGAAGAAGTTAGCTGATGATGGTA
TGGGTGTTTGTCATCCCATGACTTACCCTTATGCTGTTTGCTTTAGTCATAAATTAGTAA
AAACTAGTGCATATTTTGTTCCTTTGGAGGGTGAAGATGGGGCTAGAGTTAAAGCAGTTG
CTGTGTGCCACAGAGACACGTCGAAATGGGATCCAAATCATGTATCGTTCCCAGACCTCA
ATGTCAAGCCTGGAACAGTTCCAGTGTGTCATGTCTTACCTGAGAGGCATCTTCTTTGGG
TTCCCAAGTAGAGTACTAATCAAATTTCTGCTTGTTTGCACACGGGCTTAGTGAAGCTTA
TCTGCTATTAAATAAGTTCCAGTAAGTACTATTTAGTTTACATCCAAACGCTTCTTTGTC
Ctcctcgtatatatccttaaataagtggacagatccatgtacattagctgcacatcatcc
aatctaagggggcccgaccca

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC005061A_C01 KMC005061A_c01
         (1281 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

dbj|BAC22500.1| resistant specific protein-2 [Vigna radiata]          214  3e-61
dbj|BAC22501.1| resistant specific protein-3 [Vigna radiata]          211  4e-61
dbj|BAC22498.1| resistant specific protein-1(4) [Vigna radiata] ...   211  1e-60
gb|AAL67991.1| dehydration-induced protein RD22-like protein [Go...   189  6e-57
gb|AAL26909.1|AF319165_1 dehydration-responsive protein RD22 [Pr...   186  8e-57

>dbj|BAC22500.1| resistant specific protein-2 [Vigna radiata]
          Length = 440

 Score =  214 bits (546), Expect(2) = 3e-61
 Identities = 98/170 (57%), Positives = 129/170 (75%)
 Frame = +3

Query: 579  KVLEILVVKPDSKKTEIVEKTMGGCELPSMEGEEQMCATSLESLVDFIASKLGKNVLAIS 758
            ++LEIL VKPDSK  + VEKT+  CE P+++GEE+ CATS+ES+VDF+ SKLG N    S
Sbjct: 273  EILEILAVKPDSKNAKNVEKTLNNCEEPALKGEEKHCATSVESMVDFVTSKLGNNARVTS 332

Query: 759  TEVELEKETQSQNLLVKDGVKKLADDGMGVCHPMTYPYAVCFSHKLVKTSAYFVPLEGED 938
            TE+E+E  ++ Q  +VKDGVK LA++ +  CHPM+YPY V + HK+  ++A+ VPLEGED
Sbjct: 333  TELEIE--SKFQKFIVKDGVKILAEEEIIACHPMSYPYVVFYCHKMSNSTAHVVPLEGED 390

Query: 939  GARVKAVAVCHRDTSKWDPNHVSFPDLNVKPGTVPVCHVLPERHLLWVPK 1088
            G RVKA+ +CH+DTS+WDP+HV+F  L VKPGT PVCH  P  HLLW  K
Sbjct: 391  GTRVKAIVICHKDTSQWDPDHVAFQVLKVKPGTSPVCHFFPNGHLLWYAK 440

 Score = 44.7 bits (104), Expect(2) = 3e-61
 Identities = 22/48 (45%), Positives = 29/48 (59%)
 Frame = +2

Query: 437 KAGVFFFEENLHPGTKLDLQLSKRKPAVPILPRTIADQIPFSLEKLKE 580
           K   +F EE L  G KL +   KRK + P+L R IA+ +PFS EK+ E
Sbjct: 226 KPSSYFSEEGLRRGAKLVMLFHKRKFSTPLLTREIAEHLPFSSEKINE 273

 Score = 39.3 bits (90), Expect = 0.14
 Identities = 16/35 (45%), Positives = 25/35 (70%)
 Frame = +2

Query: 44  FSLSMMVMLAYAALPPQLDWKMMLPTTPVPKAITE 148
           FSL +++M A A+LP ++ W+  LP TP+PK I +
Sbjct: 10  FSLIVILMAAQASLPSEVYWERKLPNTPIPKVIRQ 44

>dbj|BAC22501.1| resistant specific protein-3 [Vigna radiata]
          Length = 275

 Score =  211 bits (536), Expect(2) = 4e-61
 Identities = 96/170 (56%), Positives = 129/170 (75%)
 Frame = +3

Query: 579  KVLEILVVKPDSKKTEIVEKTMGGCELPSMEGEEQMCATSLESLVDFIASKLGKNVLAIS 758
            ++LEIL VKPDSK  + VE+T+  CE P+++GEE+ CATS+ES+VDF+ SKLG N    S
Sbjct: 108  EILEILAVKPDSKDAKNVEETLNHCEKPALKGEEKQCATSVESMVDFVTSKLGNNARVTS 167

Query: 759  TEVELEKETQSQNLLVKDGVKKLADDGMGVCHPMTYPYAVCFSHKLVKTSAYFVPLEGED 938
            TE+E+   ++ Q  +VKDGVK LA++ +  CHPM+YPY V + HK+  ++A+F+PLEGED
Sbjct: 168  TELEIG--SKFQKFIVKDGVKILAEEKIIACHPMSYPYVVFYCHKMANSTAHFLPLEGED 225

Query: 939  GARVKAVAVCHRDTSKWDPNHVSFPDLNVKPGTVPVCHVLPERHLLWVPK 1088
            G RVKAVA+CH+DTS+WDP+HV+F  L VKPGT   CH  PE HL+W  K
Sbjct: 226  GTRVKAVAICHKDTSQWDPHHVAFQVLKVKPGTSSACHFFPEGHLVWYAK 275

 Score = 50.8 bits (120), Expect = 5e-05
 Identities = 23/44 (52%), Positives = 32/44 (72%)
 Frame = +2

Query: 44  FSLSMMVMLAYAALPPQLDWKMMLPTTPVPKAITEQLSLGDIVS 175
           FSL +++M A AALPP++ W+ MLP TP+PK I +   LG IV+
Sbjct: 10  FSLIVILMAAQAALPPEVYWERMLPNTPIPKVIRQFSELGPIVN 53

 Score = 47.8 bits (112), Expect(2) = 4e-61
 Identities = 25/69 (36%), Positives = 36/69 (51%)
 Frame = +2

Query: 374 KSVMEYKKLVLVFTSRCTQKTKAGVFFFEENLHPGTKLDLQLSKRKPAVPILPRTIADQI 553
           K + ++ +L  +         K   FF EE L  G KLD+   KR  + P+L R IA+ +
Sbjct: 40  KVIRQFSELGPIVNHHHHDHLKPSNFFSEERLRRGAKLDVLFRKRNFSTPLLTREIAEHL 99

Query: 554 PFSLEKLKE 580
           PFS EK+ E
Sbjct: 100 PFSSEKINE 108

>dbj|BAC22498.1| resistant specific protein-1(4) [Vigna radiata]
            gi|24416616|dbj|BAC22499.1| resistant specific
            protein-1(8) [Vigna radiata]
          Length = 402

 Score =  211 bits (536), Expect(2) = 1e-60
 Identities = 96/170 (56%), Positives = 129/170 (75%)
 Frame = +3

Query: 579  KVLEILVVKPDSKKTEIVEKTMGGCELPSMEGEEQMCATSLESLVDFIASKLGKNVLAIS 758
            ++LEIL VKPDSK  + VE+T+  CE P+++GEE+ CATS+ES+VDF+ SKLG N    S
Sbjct: 235  EILEILAVKPDSKDAKNVEETLNHCEKPALKGEEKQCATSVESMVDFVTSKLGNNARVTS 294

Query: 759  TEVELEKETQSQNLLVKDGVKKLADDGMGVCHPMTYPYAVCFSHKLVKTSAYFVPLEGED 938
            TE+E+   ++ Q  +VKDGVK LA++ +  CHPM+YPY V + HK+  ++A+F+PLEGED
Sbjct: 295  TELEIG--SKFQKFIVKDGVKILAEEKIIACHPMSYPYVVFYCHKMANSTAHFLPLEGED 352

Query: 939  GARVKAVAVCHRDTSKWDPNHVSFPDLNVKPGTVPVCHVLPERHLLWVPK 1088
            G RVKAVA+CH+DTS+WDP+HV+F  L VKPGT   CH  PE HL+W  K
Sbjct: 353  GTRVKAVAICHKDTSQWDPHHVAFQVLKVKPGTSSACHFFPEGHLVWYAK 402

 Score = 46.6 bits (109), Expect(2) = 1e-60
 Identities = 23/48 (47%), Positives = 29/48 (59%)
 Frame = +2

Query: 437 KAGVFFFEENLHPGTKLDLQLSKRKPAVPILPRTIADQIPFSLEKLKE 580
           K   FF EE L  G KLD+   KR  + P+L R IA+ +PFS EK+ E
Sbjct: 188 KPSNFFSEERLRRGAKLDVLFRKRNFSTPLLTREIAEHLPFSSEKINE 235

 Score = 45.8 bits (107), Expect = 0.001
 Identities = 19/35 (54%), Positives = 27/35 (76%)
 Frame = +2

Query: 44  FSLSMMVMLAYAALPPQLDWKMMLPTTPVPKAITE 148
           FSL +++M A AALPP++ W+ MLP TP+PK I +
Sbjct: 10  FSLIVILMAAQAALPPEVYWERMLPNTPIPKVIRQ 44

>gb|AAL67991.1| dehydration-induced protein RD22-like protein [Gossypium hirsutum]
          Length = 335

 Score =  189 bits (479), Expect(2) = 6e-57
 Identities = 90/163 (55%), Positives = 114/163 (69%)
 Frame = +3

Query: 600  VKPDSKKTEIVEKTMGGCELPSMEGEEQMCATSLESLVDFIASKLGKNVLAISTEVELEK 779
            VKP S K E+++ T+  CE P++EGEE+ CATSLES++D+  SKLGK   A+STEVE  K
Sbjct: 175  VKPGSLKAEMMKNTIKECEQPAIEGEEKYCATSLESMIDYSISKLGKVDQAVSTEVE--K 232

Query: 780  ETQSQNLLVKDGVKKLADDGMGVCHPMTYPYAVCFSHKLVKTSAYFVPLEGEDGARVKAV 959
            +T  Q   +  GV+K+ DD   VCH   Y YAV + HK   T AY VPLEG DG + KAV
Sbjct: 233  QTPMQKYTIAAGVQKMTDDKAVVCHKQNYAYAVFYCHKSETTRAYMVPLEGADGTKAKAV 292

Query: 960  AVCHRDTSKWDPNHVSFPDLNVKPGTVPVCHVLPERHLLWVPK 1088
            AVCH DTS W+P H++F  L V+PGT+PVCH LP  H++WVPK
Sbjct: 293  AVCHTDTSAWNPKHLAFQVLKVEPGTIPVCHFLPRDHIVWVPK 335

 Score = 55.8 bits (133), Expect(2) = 6e-57
 Identities = 49/181 (27%), Positives = 77/181 (42%), Gaps = 1/181 (0%)
 Frame = +2

Query: 41  LFSLSMMVMLAYAALPPQLDWKMMLPTTPVPKAITEQLSLGDIVSRGKSVN-DAETQSQN 217
           L  L++ V++++AAL P+  W   LP TP+PKA+ E L    +  +  SVN      + N
Sbjct: 8   LACLALAVVVSHAALSPEQYWSYKLPNTPMPKAVKEILHPELMEEKSTSVNVGGGGVNVN 67

Query: 218 MFHGF*GRFHHNEIGSCFGCNVHSSFLVYRHNILYGV*TLCAKADPSFMHDTKSVMEYKK 397
              G  G   H  +G   G  V++           G  T     DP           +  
Sbjct: 68  TGKGKPGGDTHVNVGG-KGVGVNTG--------KPGGGTHVNVGDP-----------FNY 107

Query: 398 LVLVFTSRCTQKTKAGVFFFEENLHPGTKLDLQLSKRKPAVPILPRTIADQIPFSLEKLK 577
           L     ++  +     +FF E+++HPG  + L  ++       LP   A +IPFS +KL 
Sbjct: 108 LYAASETQIHEDPNVALFFLEKDMHPGATMSLHFTENTEKSAFLPYQTAQKIPFSSDKLP 167

Query: 578 E 580
           E
Sbjct: 168 E 168

>gb|AAL26909.1|AF319165_1 dehydration-responsive protein RD22 [Prunus persica]
          Length = 349

 Score =  186 bits (473), Expect(2) = 8e-57
 Identities = 93/166 (56%), Positives = 113/166 (68%)
 Frame = +3

Query: 600  VKPDSKKTEIVEKTMGGCELPSMEGEEQMCATSLESLVDFIASKLGKNVLAISTEVELEK 779
            VKP+S + +I++ T+  CE   + GEE+ CATSLES+VDF  SKLG N+ AISTE E  K
Sbjct: 186  VKPESVEADIIKGTIEECESSGIRGEEKYCATSLESMVDFSTSKLGGNIQAISTEAE--K 243

Query: 780  ETQSQNLLVKDGVKKLADDGMGVCHPMTYPYAVCFSHKLVKTSAYFVPLEGEDGARVKAV 959
                Q   +  GVKKLA     VCH  TYPYAV + H    T AY VPL+G DG   KAV
Sbjct: 244  GATLQKYTITPGVKKLAAGKSVVCHKQTYPYAVFYCHATKTTRAYVVPLKGADGLEAKAV 303

Query: 960  AVCHRDTSKWDPNHVSFPDLNVKPGTVPVCHVLPERHLLWVPK*ST 1097
            AVCH DTS+W+P H++F  L VKPGTVPVCH LP+ H++WVPK ST
Sbjct: 304  AVCHTDTSEWNPKHLAFQVLKVKPGTVPVCHFLPKDHIVWVPKKST 349

 Score = 57.8 bits (138), Expect(2) = 8e-57
 Identities = 49/181 (27%), Positives = 76/181 (41%), Gaps = 8/181 (4%)
 Frame = +2

Query: 62  VMLAYAALPPQLDWKMMLPTTPVPKAITEQLSLGDIVSRGKSVN----DAETQSQNMFHG 229
           ++ ++AALPPQL W  +LP T +PK+I+E L       +G +V         Q+     G
Sbjct: 1   LVASHAALPPQLYWNSVLPNTQMPKSISELLQPDFAEEKGTTVGVGKGGVNVQAGKGKPG 60

Query: 230 F*GRFHHNEIGSCFGCNVHSSFLVYRHNILYGV*TLCA----KADPSFMHDTKSVMEYKK 397
             G  + N  G   G  V +       N+  G   +      K  P ++        +  
Sbjct: 61  --GGTNVNVGGKGGGVGVTTGKPGGGTNVGVGKGGVSVNTGHKGKPVYVGVKPGPNPFNY 118

Query: 398 LVLVFTSRCTQKTKAGVFFFEENLHPGTKLDLQLSKRKPAVPILPRTIADQIPFSLEKLK 577
           +     ++      A +FF E+++ PGT + L  S        +PR  AD IPFS  KL 
Sbjct: 119 VYAATETQLHGNRNAAIFFLEKDIRPGTSMTLTFSGNSNTAAFVPRKTADSIPFSSNKLP 178

Query: 578 E 580
           E
Sbjct: 179 E 179

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,137,066,385
Number of Sequences: 1393205
Number of extensions: 25675345
Number of successful extensions: 58782
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 55492
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 58718
length of database: 448,689,247
effective HSP length: 126
effective length of database: 273,145,417
effective search space used: 81943625100
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPD027h08_f AV771878 1 562 2 MPD022e06_f AV771522 301 847 3 MPD083h10_f AV775482 367 939 4 MPD007g03_f AV770483 809 1288

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