Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC005059A_C02 KMC005059A_c02
(592 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAC54828.1| vacuolar processing enzyme-1b [Nicotiana tabacum] 99 9e-26
dbj|BAA76744.1| asparaginyl endopeptidase (VmPE-1) [Vigna mungo] 97 3e-25
sp|P49044|VPE_VICSA Vacuolar processing enzyme precursor (VPE) (... 100 7e-25
emb|CAC43295.1| putative vacuolar processing enzyme [Beta vulgaris] 100 1e-24
dbj|BAC54827.1| vacuolar processing enzyme-1a [Nicotiana tabacum] 98 6e-24
>dbj|BAC54828.1| vacuolar processing enzyme-1b [Nicotiana tabacum]
Length = 489
Score = 99.4 bits (246), Expect(2) = 9e-26
Identities = 46/99 (46%), Positives = 66/99 (66%)
Frame = -3
Query: 575 SELVSQRDARLFYLRHKLEKAMDGSNEKLQAQKELDAEMAHREHVDKSVHLIGNLFFGED 396
S+ V+QRDA L + HK A +GS K++AQK+L+ ++HR H+D SV L+G L FG +
Sbjct: 344 SKAVNQRDADLLHFWHKFRTAPEGSVRKIEAQKQLNEAISHRVHLDNSVALVGKLLFGIE 403
Query: 395 KSSNTMLHVRPAGQPLVDDWECFKTLIKTYKKPLRELYQ 279
K + VRPAGQPLVDDW+C K+ ++T++ L Q
Sbjct: 404 KGPEVLSGVRPAGQPLVDDWDCLKSFVRTFETHCGSLSQ 442
Score = 39.3 bits (90), Expect(2) = 9e-26
Identities = 19/47 (40%), Positives = 30/47 (63%), Gaps = 6/47 (12%)
Frame = -2
Query: 303 EATARALSRYGRKYTRAFAYMCNAGISEKQLIAA------SLPSLSW 181
E +LS+YG K+ R+ A +CNAGI ++Q++ A S+PS +W
Sbjct: 434 ETHCGSLSQYGMKHMRSIANICNAGIKKEQMVEASAQACPSVPSNTW 480
>dbj|BAA76744.1| asparaginyl endopeptidase (VmPE-1) [Vigna mungo]
Length = 483
Score = 97.4 bits (241), Expect(2) = 3e-25
Identities = 49/100 (49%), Positives = 65/100 (65%)
Frame = -3
Query: 578 PSELVSQRDARLFYLRHKLEKAMDGSNEKLQAQKELDAEMAHREHVDKSVHLIGNLFFGE 399
PS+ V+QRDA L + K KA GS+ K AQK++ M+HR H+D SV LIG L FG
Sbjct: 337 PSKAVNQRDADLVHFWDKFPKAPLGSSRKSVAQKQILEAMSHRMHIDDSVTLIGKLLFGI 396
Query: 398 DKSSNTMLHVRPAGQPLVDDWECFKTLIKTYKKPLRELYQ 279
++ + VRPAGQPLVDDW+C KTL++T++ L Q
Sbjct: 397 EEGPELLSSVRPAGQPLVDDWDCLKTLVRTFETHCGSLSQ 436
Score = 39.3 bits (90), Expect(2) = 3e-25
Identities = 19/47 (40%), Positives = 29/47 (61%), Gaps = 6/47 (12%)
Frame = -2
Query: 303 EATARALSRYGRKYTRAFAYMCNAGISEKQLIAA------SLPSLSW 181
E +LS+YG K+ R+FA +CNAGI ++Q+ A S+P+ W
Sbjct: 428 ETHCGSLSQYGMKHMRSFANLCNAGIRKEQMAEASAQACVSIPATPW 474
>sp|P49044|VPE_VICSA Vacuolar processing enzyme precursor (VPE) (Proteinase B)
gi|1076553|pir||S49175 legumain (EC 3.4.22.34) precursor
[similarity] - spring vetch gi|510358|emb|CAA84383.1|
cysteine proteinase [Vicia sativa]
Length = 493
Score = 99.8 bits (247), Expect(2) = 7e-25
Identities = 47/100 (47%), Positives = 68/100 (68%)
Frame = -3
Query: 578 PSELVSQRDARLFYLRHKLEKAMDGSNEKLQAQKELDAEMAHREHVDKSVHLIGNLFFGE 399
PS V+QRDA L + K KA +GS++K +A+K++ M+HR+H+D SV LIG L FG
Sbjct: 347 PSAAVNQRDADLIHFWEKFRKAPEGSSQKNEAEKQVLEAMSHRKHIDNSVKLIGQLLFGI 406
Query: 398 DKSSNTMLHVRPAGQPLVDDWECFKTLIKTYKKPLRELYQ 279
+K + + VRPAG PLVD+W+C KT++KT++ L Q
Sbjct: 407 EKGTELLDVVRPAGSPLVDNWDCLKTMVKTFETHCGSLSQ 446
Score = 35.8 bits (81), Expect(2) = 7e-25
Identities = 18/47 (38%), Positives = 27/47 (57%), Gaps = 6/47 (12%)
Frame = -2
Query: 303 EATARALSRYGRKYTRAFAYMCNAGISEKQL------IAASLPSLSW 181
E +LS+YG K+ R+FA +CNAGI + + AS+P+ W
Sbjct: 438 ETHCGSLSQYGMKHMRSFANICNAGIPNEPMAEASAQACASIPANPW 484
>emb|CAC43295.1| putative vacuolar processing enzyme [Beta vulgaris]
Length = 486
Score = 100 bits (248), Expect(2) = 1e-24
Identities = 48/99 (48%), Positives = 63/99 (63%)
Frame = -3
Query: 575 SELVSQRDARLFYLRHKLEKAMDGSNEKLQAQKELDAEMAHREHVDKSVHLIGNLFFGED 396
S V+QRDA L + +K KA +GS K+ AQK+ M+HR H+D S+ LIG L FG +
Sbjct: 341 SNAVNQRDADLIHFWNKFRKASEGSQRKINAQKQFMEVMSHRVHLDDSIKLIGKLLFGIE 400
Query: 395 KSSNTMLHVRPAGQPLVDDWECFKTLIKTYKKPLRELYQ 279
K + VRP GQPLVDDW C KTL++T++K L Q
Sbjct: 401 KGLGVLQTVRPTGQPLVDDWNCLKTLVRTFEKHCGSLSQ 439
Score = 35.0 bits (79), Expect(2) = 1e-24
Identities = 15/30 (50%), Positives = 22/30 (73%)
Frame = -2
Query: 288 ALSRYGRKYTRAFAYMCNAGISEKQLIAAS 199
+LS+YG K+ R+ A +CNAGI+ Q+ AS
Sbjct: 436 SLSQYGMKHMRSIANICNAGITTNQMAEAS 465
>dbj|BAC54827.1| vacuolar processing enzyme-1a [Nicotiana tabacum]
Length = 490
Score = 98.2 bits (243), Expect(2) = 6e-24
Identities = 45/99 (45%), Positives = 66/99 (66%)
Frame = -3
Query: 575 SELVSQRDARLFYLRHKLEKAMDGSNEKLQAQKELDAEMAHREHVDKSVHLIGNLFFGED 396
S+ V+QRDA L + +K KA +GS K++AQK+L+ ++HR H+D S+ L+G L FG
Sbjct: 345 SKAVNQRDADLLHFWYKFRKAPEGSVRKIEAQKQLNEAISHRVHLDNSIALVGKLLFGIK 404
Query: 395 KSSNTMLHVRPAGQPLVDDWECFKTLIKTYKKPLRELYQ 279
K + VRPAGQPLVDDW+C K+ ++T++ L Q
Sbjct: 405 KGPEVLSSVRPAGQPLVDDWDCLKSFVRTFETHCGSLSQ 443
Score = 34.3 bits (77), Expect(2) = 6e-24
Identities = 15/35 (42%), Positives = 22/35 (62%)
Frame = -2
Query: 303 EATARALSRYGRKYTRAFAYMCNAGISEKQLIAAS 199
E +LS+YG K+ R+ A +CN GI Q++ AS
Sbjct: 435 ETHCGSLSQYGMKHMRSIANICNVGIKMAQMVEAS 469
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 503,492,269
Number of Sequences: 1393205
Number of extensions: 10913265
Number of successful extensions: 25615
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 25004
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25603
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 22569056698
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
