>KMC005041A_C01 KMC005041A_c01
ggttaggggaaaaaagaagtaaaTTTTACTAGAATTACAAATGCATTAATCTGGAAATTT
ACAGAATCGTCAAAATGATCTCTTCTGATTATATTCTATATGACATTATCTTTATCCCAT
TTTTTAACTTAAAAAGACTTGTTGAGGTGTTAATAACACTGGCTGAAGCAGCAAAGAAAA
CCATTAGACTTGATTTCATCCTTTGCTCTGGGTGTGTCGCCTGTGTCATCACGTTTGGCC
ATTAAGGACCTAGTACCAGGGGACTCTGGCGGAAAGACTGTGTTCTCAGGGGTGAATATT
CTTGAAGGAATCACAGGTTCAGGAGGTGAAACAGTGTAAAGATAGTTTAGGAGCATTTGG
ATTATCCGAGTGAAGTTGGGTCTGGCATTTGGGTCCTCTTGCCAGCATGAAGTAAGAATT
ACAGCCAATTCCTCAGGAAGATTCTCTGCACTTGGCCTTACACTCTTAAAGGCTGCAGCA
TATGCTGCCTGTAGGTTTGACATGCCTTCAAAAGGAACTTTGTTGTGTAAGAGCTCCCAA
AACACGATTGCGAAACTATAAGCATCGACTTTATGGTTGTAATGCTTCTTCTCCCCCTG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC005041A_C01 KMC005041A_c01
         (599 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_199829.1| protein kinase ATN1-like protein; protein id: A...   230  1e-59
ref|NP_198870.1| protein kinase - like protein; protein id: At5g...   198  5e-50
pir||S61766 protein kinase ATN1 (EC 2.7.1.-) - Arabidopsis thali...   192  2e-48
ref|NP_189393.1| putative protein kinase, ATN1; protein id: At3g...   192  3e-48
gb|AAM98213.1| protein kinase ATN1-like protein [Arabidopsis tha...   130  1e-29

>ref|NP_199829.1| protein kinase ATN1-like protein; protein id: At5g50180.1
           [Arabidopsis thaliana] gi|9759020|dbj|BAB09389.1|
           protein kinase ATN1-like protein [Arabidopsis thaliana]
           gi|28416673|gb|AAO42867.1| At5g50180 [Arabidopsis
           thaliana]
          Length = 346

 Score =  230 bits (586), Expect = 1e-59
 Identities = 108/148 (72%), Positives = 121/148 (80%)
 Frame = -1

Query: 596 GEKKHYNHKVDAYSFAIVFWELLHNKVPFEGMSNLQAAYAAAFKSVRPSAENLPEELAVI 417
           GEKKHYNHKVDAYSFAIV WELLHNK+PFEGMSNLQAAYAAAFK+VRPSAE+LPEEL  I
Sbjct: 199 GEKKHYNHKVDAYSFAIVLWELLHNKLPFEGMSNLQAAYAAAFKNVRPSAESLPEELGDI 258

Query: 416 LTSCWQEDPNARPNFTRIIQMLLNYLYTVSPPEPVIPSRIFTPENTVFPPESPGTRSLMA 237
           +TSCW EDPNARPNFT II++LLNYL  V  P   IP RI   +NT+ PP+SPGT SLMA
Sbjct: 259 VTSCWNEDPNARPNFTHIIELLLNYLSKVGSPISAIPQRILASKNTLLPPDSPGTSSLMA 318

Query: 236 KRDDTGDTPRAKDEIKSNGFLCCFSQCY 153
           K D+ G+TP+AK E K  G   CF+QCY
Sbjct: 319 KLDECGETPKAKSEDKRKGLFFCFNQCY 346

>ref|NP_198870.1| protein kinase - like protein; protein id: At5g40540.1 [Arabidopsis
           thaliana] gi|9758080|dbj|BAB08524.1| protein kinase ATN1
           [Arabidopsis thaliana]
          Length = 353

 Score =  198 bits (503), Expect = 5e-50
 Identities = 99/148 (66%), Positives = 118/148 (78%), Gaps = 5/148 (3%)
 Frame = -1

Query: 596 GEKKHYNHKVDAYSFAIVFWELLHNKVPFEGMSNLQAAYAAAFKSVRPSAENLPEELAVI 417
           GEKKHYNHKVDAYSFAIV WEL+HNK+PFEGMSNLQAAYAAAFK+VRPSA++LP++LA+I
Sbjct: 205 GEKKHYNHKVDAYSFAIVLWELIHNKLPFEGMSNLQAAYAAAFKNVRPSADDLPKDLAMI 264

Query: 416 LTSCWQEDPNARPNFTRIIQMLLNYLYTVSPPEPVIPS--RIFTPENTVFPPESPGTRSL 243
           +TSCW+EDPN RPNFT IIQMLL  L T+S  E V P+  R+F+ ENTV PPESPGT SL
Sbjct: 265 VTSCWKEDPNDRPNFTEIIQMLLRCLSTISSTELVPPAIKRVFSSENTVLPPESPGTCSL 324

Query: 242 MAKRDD---TGDTPRAKDEIKSNGFLCC 168
           M  RD      D   A++E++ + F  C
Sbjct: 325 MTVRDKDQIPTDANSAQNEVRGSFFFFC 352

>pir||S61766 protein kinase ATN1 (EC 2.7.1.-) - Arabidopsis thaliana
           gi|1054633|emb|CAA63387.1| protein kinase [Arabidopsis
           thaliana]
          Length = 356

 Score =  192 bits (489), Expect = 2e-48
 Identities = 99/154 (64%), Positives = 117/154 (75%), Gaps = 10/154 (6%)
 Frame = -1

Query: 599 QGEKKHYNHKVDAYSFAIVFWELLHNKVPFEGMSNLQAAYAAAFKSVRPSAENLPEELAV 420
           QGEKKHYNHKVDAYSFAIV WEL+ NK+PFEGMSNLQAAYAAAFK++RPSAE+LP +L +
Sbjct: 204 QGEKKHYNHKVDAYSFAIVLWELILNKLPFEGMSNLQAAYAAAFKNLRPSAEDLPGDLEM 263

Query: 419 ILTSCWQEDPNARPNFTRIIQMLLNYLYTVSPPE--PVIPSRIFTPENTVFPPESPGTRS 246
           I+TSCW+EDPN RPNFT IIQMLL YL TVS P+  P +  R+F+ EN V  PESPGT S
Sbjct: 264 IVTSCWKEDPNERPNFTEIIQMLLRYLTTVSAPQIIPPLNRRVFSSENIVLSPESPGTCS 323

Query: 245 LMAKRDDTGDTPR--------AKDEIKSNGFLCC 168
           LM+ RD  GD  R        ++ + K + F CC
Sbjct: 324 LMSVRD--GDVSRQTVNTADSSEKQTKGSFFSCC 355

>ref|NP_189393.1| putative protein kinase, ATN1; protein id: At3g27560.1, supported
           by cDNA: gi_16604327, supported by cDNA: gi_19699189
           [Arabidopsis thaliana] gi|11994183|dbj|BAB01286.1|
           nearly identical to protein kinase ATN1 [Arabidopsis
           thaliana] gi|16604328|gb|AAL24170.1| AT3g27560/MMJ24_11
           [Arabidopsis thaliana] gi|19699190|gb|AAL90961.1|
           AT3g27560/MMJ24_11 [Arabidopsis thaliana]
          Length = 356

 Score =  192 bits (488), Expect = 3e-48
 Identities = 99/154 (64%), Positives = 117/154 (75%), Gaps = 10/154 (6%)
 Frame = -1

Query: 599 QGEKKHYNHKVDAYSFAIVFWELLHNKVPFEGMSNLQAAYAAAFKSVRPSAENLPEELAV 420
           QGEKKHYNHKVDAYSFAIV WEL+ NK+PFEGMSNLQAAYAAAFK++RPSAE+LP +L +
Sbjct: 204 QGEKKHYNHKVDAYSFAIVLWELILNKLPFEGMSNLQAAYAAAFKNLRPSAEDLPGDLEM 263

Query: 419 ILTSCWQEDPNARPNFTRIIQMLLNYLYTVSPPEPVIP--SRIFTPENTVFPPESPGTRS 246
           I+TSCW+EDPN RPNFT IIQMLL YL TVS P+ + P   R+F+ EN V  PESPGT S
Sbjct: 264 IVTSCWKEDPNERPNFTEIIQMLLRYLTTVSAPQIIPPPNRRVFSSENIVLSPESPGTCS 323

Query: 245 LMAKRDDTGDTPR--------AKDEIKSNGFLCC 168
           LM+ RD  GD  R        ++ + K + F CC
Sbjct: 324 LMSVRD--GDVSRQTVNTADSSEKQTKGSFFSCC 355

>gb|AAM98213.1| protein kinase ATN1-like protein [Arabidopsis thaliana]
           gi|25084113|gb|AAN72179.1| protein kinase ATN1-like
           protein [Arabidopsis thaliana]
          Length = 333

 Score =  130 bits (328), Expect = 1e-29
 Identities = 62/96 (64%), Positives = 75/96 (77%), Gaps = 1/96 (1%)
 Frame = -1

Query: 599 QGEKKHYNHKVDAYSFAIVFWELLHNKVPFEGMSNLQAAYAAAFKSVRP-SAENLPEELA 423
           QGEKKHYN+KVD YSF IV WELL N++PFEGMSNLQAAYAAAFK  RP   E +   LA
Sbjct: 196 QGEKKHYNNKVDVYSFGIVLWELLTNRMPFEGMSNLQAAYAAAFKQERPVMPEGISPSLA 255

Query: 422 VILTSCWQEDPNARPNFTRIIQMLLNYLYTVSPPEP 315
            I+ SCW EDPN RP+F++II++L  +L T++PP P
Sbjct: 256 FIVQSCWVEDPNMRPSFSQIIRLLNEFLLTLTPPPP 291

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 502,688,420
Number of Sequences: 1393205
Number of extensions: 10911277
Number of successful extensions: 31719
Number of sequences better than 10.0: 2271
Number of HSP's better than 10.0 without gapping: 29339
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30642
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23426109484
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPD005f08_f AV770344 1 516 2 MFB094e03_f BP040853 24 579 3 MFB076f11_f BP039570 28 437 4 MPDL016c11_f AV777305 28 585 5 SPD013c08_f BP045020 29 138 6 MFB001a07_f BP033939 53 599

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