>KMC005025A_C01 KMC005025A_c01
ctttttttgTTTTGATCTGAATGAAAAGATCTGTGGTTGTGCTTCAGAAAACTAATCTGA
ATGCAAAGATGAAACAACGGGTTTCATTCAGCAGTTGCTTTATTTATCCCAAAATTCATA
TCATGGAGCAGGGTGGGTAAACCAGAAAAAAAAAAAGGAAAAAAAATCTAAAACAATCTA
AATTATAAGCTCTACGCATCAACAGTCTCCTCAGCATCAACAGAAGCCAAAACAAGGCTC
GTGGCTCTTCCCTCACAGCTTCACATGTTCCATCAATTAGGCATGTGTTCTTCCCCAATG
CCTTCAACGCCAAATGGGCTGCTTTCTGCCCAGATATCATCATAGCACCGAAAGTCGGAC
CCGAGTGCTGGGGGCACCATCAATTTCAGCTACCTCCATGCCAGTCACTATCATTCCTGG
TACAACCTCCCTGGTGAACCTAACAATAGCGTCTGTCAGCTGTGTTCATATCCAGTGCCT
TCATTCCAGGAACACTGTCGATCATGCCAATGCTCTTCAACCTCTTTACTCCAGTTGCAC
CGAAGGGCCCATCATGGCCACAGGAACTCACAACAATCTTAGCCTCCATAACGTTTGGGT
CCATGCATGACTGTGTGTCATGGTTCATTGACACCAAAGCCCAGTTGGTAACCACCCCAG
CAAC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC005025A_C01 KMC005025A_c01
         (664 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

sp|Q41738|TH41_MAIZE Thiazole biosynthetic enzyme 1-1, chloropla...   165  6e-40
sp|O23787|THI4_CITSI Thiazole biosynthetic enzyme, chloroplast p...   164  8e-40
dbj|BAC45141.1| putative thiamine biosynthetic enzyme [Oryza sat...   163  2e-39
sp|Q41739|TH42_MAIZE Thiazole biosynthetic enzyme 1-2, chloropla...   163  2e-39
sp|Q38709|THI4_ALNGL Thiazole biosynthetic enzyme, chloroplast p...   161  8e-39

>sp|Q41738|TH41_MAIZE Thiazole biosynthetic enzyme 1-1, chloroplast precursor
           gi|2130146|pir||S61419 thiamin biosynthesis protein
           thi1-1 - maize gi|596078|gb|AAA96738.1| thiamine
           biosynthetic enzyme
          Length = 354

 Score =  165 bits (417), Expect = 6e-40
 Identities = 94/149 (63%), Positives = 104/149 (69%), Gaps = 9/149 (6%)
 Frame = -1

Query: 664 VAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSVPG 485
           V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDGPFGATGVKRL+ IGMI +VPG
Sbjct: 203 VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLQDIGMISAVPG 262

Query: 484 MKALDMNTADRRYC*VHQ--------GGCTRNDSDWHGGS*N*WCPQHSGPTFGAMMISG 329
           MKALDMNTA+     + +         G    + D              GPTFGAMMISG
Sbjct: 263 MKALDMNTAEDEIVRLTREVVPGMIVTGMEVAEIDG---------APRMGPTFGAMMISG 313

Query: 328 QKAAHLALKALGKNTCLIDGTCE-AVREE 245
           QKAAHLALKALG+    +DGT    +REE
Sbjct: 314 QKAAHLALKALGRPNA-VDGTMSPPLREE 341

 Score = 53.9 bits (128), Expect = 2e-06
 Identities = 26/28 (92%), Positives = 26/28 (92%)
 Frame = -2

Query: 453 DAIVRFTREVVPGMIVTGMEVAEIDGAP 370
           D IVR TREVVPGMIVTGMEVAEIDGAP
Sbjct: 273 DEIVRLTREVVPGMIVTGMEVAEIDGAP 300

>sp|O23787|THI4_CITSI Thiazole biosynthetic enzyme, chloroplast precursor
           gi|7446894|pir||T10474 thiamin biosynthesis protein thi1
           - sweet orange gi|2582665|emb|CAB05370.1| thi [Citrus
           sinensis]
          Length = 356

 Score =  164 bits (416), Expect = 8e-40
 Identities = 92/141 (65%), Positives = 101/141 (71%), Gaps = 8/141 (5%)
 Frame = -1

Query: 664 VAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSVPG 485
           V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDGPFGATGVKRLKSIGMI+ VPG
Sbjct: 204 VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLKSIGMIEEVPG 263

Query: 484 MKALDMNTADRRYC*VHQ--------GGCTRNDSDWHGGS*N*WCPQHSGPTFGAMMISG 329
           MKALDMN+A+     + +         G    + D              GPTFGAMMISG
Sbjct: 264 MKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDG---------APRMGPTFGAMMISG 314

Query: 328 QKAAHLALKALGKNTCLIDGT 266
           QKAAHLALK+LG+   L DGT
Sbjct: 315 QKAAHLALKSLGQPNAL-DGT 334

 Score = 55.8 bits (133), Expect = 5e-07
 Identities = 27/28 (96%), Positives = 27/28 (96%)
 Frame = -2

Query: 453 DAIVRFTREVVPGMIVTGMEVAEIDGAP 370
           DAIVR TREVVPGMIVTGMEVAEIDGAP
Sbjct: 274 DAIVRLTREVVPGMIVTGMEVAEIDGAP 301

>dbj|BAC45141.1| putative thiamine biosynthetic enzyme [Oryza sativa (japonica
           cultivar-group)]
          Length = 352

 Score =  163 bits (413), Expect = 2e-39
 Identities = 90/141 (63%), Positives = 101/141 (70%), Gaps = 8/141 (5%)
 Frame = -1

Query: 664 VAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSVPG 485
           V GVVTNWALVSMNHDTQSCMDPNVME+++VVSSCGHDGPFGATGVKRL+ IGMID+VPG
Sbjct: 196 VGGVVTNWALVSMNHDTQSCMDPNVMESRVVVSSCGHDGPFGATGVKRLQDIGMIDAVPG 255

Query: 484 MKALDMNTADRRYC*VHQ--------GGCTRNDSDWHGGS*N*WCPQHSGPTFGAMMISG 329
           M+ALDMNTA+     + +         G    + D              GPTFGAMMISG
Sbjct: 256 MRALDMNTAEDEIVRLTREVVPGMIVTGMEVAEIDG---------APRMGPTFGAMMISG 306

Query: 328 QKAAHLALKALGKNTCLIDGT 266
           QKAAHLALKALG+    IDGT
Sbjct: 307 QKAAHLALKALGRPNA-IDGT 326

 Score = 53.9 bits (128), Expect = 2e-06
 Identities = 26/28 (92%), Positives = 26/28 (92%)
 Frame = -2

Query: 453 DAIVRFTREVVPGMIVTGMEVAEIDGAP 370
           D IVR TREVVPGMIVTGMEVAEIDGAP
Sbjct: 266 DEIVRLTREVVPGMIVTGMEVAEIDGAP 293

>sp|Q41739|TH42_MAIZE Thiazole biosynthetic enzyme 1-2, chloroplast precursor
           gi|2130147|pir||S61420 thiamin biosynthesis protein
           thi1-2 - maize gi|596080|gb|AAA96739.1| thiamine
           biosynthetic enzyme
          Length = 354

 Score =  163 bits (413), Expect = 2e-39
 Identities = 94/152 (61%), Positives = 104/152 (67%), Gaps = 12/152 (7%)
 Frame = -1

Query: 664 VAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSVPG 485
           V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDGPFGATGVKRL+ IGMI +VPG
Sbjct: 200 VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLQDIGMISAVPG 259

Query: 484 MKALDMNTADRRYC*VHQ--------GGCTRNDSDWHGGS*N*WCPQHSGPTFGAMMISG 329
           MKALDMN A+     + +         G    + D              GPTFGAMMISG
Sbjct: 260 MKALDMNAAEDEIVRLTREVVPGMIVTGMEVAEIDG---------APRMGPTFGAMMISG 310

Query: 328 QKAAHLALKALGKNTCLIDGT----CEAVREE 245
           QKAAHLALKALG+    +DGT      A+REE
Sbjct: 311 QKAAHLALKALGRPNA-VDGTIPEVSPALREE 341

 Score = 53.9 bits (128), Expect = 2e-06
 Identities = 26/28 (92%), Positives = 26/28 (92%)
 Frame = -2

Query: 453 DAIVRFTREVVPGMIVTGMEVAEIDGAP 370
           D IVR TREVVPGMIVTGMEVAEIDGAP
Sbjct: 270 DEIVRLTREVVPGMIVTGMEVAEIDGAP 297

>sp|Q38709|THI4_ALNGL Thiazole biosynthetic enzyme, chloroplast precursor (AG6)
           gi|1289204|emb|CAA66064.1| thaizole biosynthetic enzmye
           [Alnus glutinosa]
          Length = 352

 Score =  161 bits (407), Expect = 8e-39
 Identities = 91/141 (64%), Positives = 99/141 (69%), Gaps = 8/141 (5%)
 Frame = -1

Query: 664 VAGVVTNWALVSMNHDTQSCMDPNVMEAKIVVSSCGHDGPFGATGVKRLKSIGMIDSVPG 485
           V GVVTNWALVSMNHDTQSCMDPNVMEAK+VVSSCGHDGPFGATGVK L+SIGMID+VPG
Sbjct: 200 VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKSLRSIGMIDTVPG 259

Query: 484 MKALDMNTADRRYC*VHQ--------GGCTRNDSDWHGGS*N*WCPQHSGPTFGAMMISG 329
           MKALDMN A+     + +         G    + D              GPTFGAMMISG
Sbjct: 260 MKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEIDG---------APRMGPTFGAMMISG 310

Query: 328 QKAAHLALKALGKNTCLIDGT 266
           QKAAHLALKALG    L DG+
Sbjct: 311 QKAAHLALKALGLPNAL-DGS 330

 Score = 55.5 bits (132), Expect = 6e-07
 Identities = 26/28 (92%), Positives = 27/28 (95%)
 Frame = -2

Query: 453 DAIVRFTREVVPGMIVTGMEVAEIDGAP 370
           DAIVR TRE+VPGMIVTGMEVAEIDGAP
Sbjct: 270 DAIVRLTREIVPGMIVTGMEVAEIDGAP 297

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 624,399,794
Number of Sequences: 1393205
Number of extensions: 14438951
Number of successful extensions: 46990
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 43744
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 46798
length of database: 448,689,247
effective HSP length: 119
effective length of database: 282,897,852
effective search space used: 28572683052
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPD086d08_f AV775644 1 493 2 MPD027h05_f AV771875 6 552 3 SPD048h04_f BP047863 16 632 4 MPD080g11_f AV775283 19 510 5 SPD069b03_f BP049493 19 494 6 SPD097f01_f BP051764 21 609 7 MWM218g03_f AV768080 38 387 8 MFB064e12_f BP038650 40 542 9 MFB037d09_f BP036705 41 643 10 MFB008g02_f BP034504 44 594 11 MFB046b05_f BP037337 44 613 12 SPD043f12_f BP047448 46 636 13 MFB033g11_f BP036448 46 537 14 MFB089g05_f BP040526 51 638 15 MPD044g12_f AV773017 51 488 16 SPD013d07_f BP045027 52 499 17 SPD059f05_f BP048708 52 655 18 MPD004f07_f AV770275 55 184 19 MPD016c07_f AV771087 55 581 20 MFB070a06_f BP039058 58 624 21 MFB008h08_f BP034517 61 579 22 SPD031f08_f BP046484 61 433 23 SPD005f02_f BP044409 61 568 24 SPD066f12_f BP049291 61 541 25 MPD095b07_f AV776203 62 527 26 MFBL042h06_f BP043410 62 545 27 MFB023f04_f BP035673 63 479 28 MFB059g10_f BP038307 64 284 29 MFB042b01_f BP037039 64 634 30 SPD041h02_f BP047292 65 368 31 MF045d06_f BP030665 65 638 32 MFB022a12_f BP035551 66 630 33 MPD068e02_f AV774523 66 587 34 SPD063a03_f BP048988 67 648 35 MFB042g06_f BP037090 67 584 36 MFB011c07_f BP034702 68 643 37 SPD094g05_f BP051535 68 571 38 MFB015g03_f BP035045 68 492 39 MFB032e09_f BP036355 68 468 40 SPD027b09_f BP046119 68 648 41 MF042e12_f BP030501 69 290 42 SPD091g06_f BP051294 69 554 43 MFB069h10_f BP039051 69 576 44 MF097f11_f BP033363 77 252 45 MFB089c12_f BP040491 77 624 46 SPD084a07_f BP050676 77 615 47 MFB097b03_f BP041038 82 605 48 MPD034h08_f AV772365 86 677 49 MWM116e07_f AV766591 88 400 50 MPD082b09_f AV775368 89 322 51 MWM161e11_f AV767234 89 411 52 SPD013a12_f BP045005 89 660 53 SPD061f12_f BP048876 89 620 54 SPD008d10_f BP044636 89 615 55 MF012d12_f BP028870 90 595 56 MFB019e03_f BP035345 92 643 57 MPD066c11_f AV774389 101 594 58 MPD018g05_f AV771264 102 627 59 MFB093h01_f BP040811 102 318 60 SPD014b01_f BP045087 102 476 61 MF050b01_f BP030910 102 625 62 MFB013d08_f BP034870 102 635 63 MFB031b03_f BP036246 104 426 64 MFB047g03_f BP037441 104 534 65 SPD074h07_f BP049946 106 366 66 SPD001a06_f BP044048 107 636 67 MFB020h10_f BP035460 109 612 68 MFB056h06_f BP038091 109 682 69 SPD038e10_f BP047032 109 671 70 MPD099a12_f AV776436 110 497

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