Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004991A_C01 KMC004991A_c01
(570 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_172648.1| glyoxalase I, putative (lactoylglutathione lyas... 286 1e-76
gb|AAL07227.1| putative lactoylglutathione lyase [Arabidopsis th... 283 8e-76
emb|CAB09799.1| hypothetical protein [Citrus x paradisi] 281 5e-75
sp|Q39366|LGUL_BRAOL Putative lactoylglutathione lyase (Methylgl... 276 2e-73
emb|CAB50787.2| putative glyoxalase I [Triticum aestivum] 266 1e-70
>ref|NP_172648.1| glyoxalase I, putative (lactoylglutathione lyase); protein id:
At1g11840.1, supported by cDNA: 39107., supported by
cDNA: gi_11094298, supported by cDNA: gi_15810218,
supported by cDNA: gi_16226212, supported by cDNA:
gi_18377844, supported by cDNA: gi_20453274 [Arabidopsis
thaliana] gi|25350142|pir||F86252 hypothetical protein
[imported] - Arabidopsis thaliana
gi|3157947|gb|AAC17630.1| Similar to protein gb|Z74962
from Brassica oleracea which is similar to bacterial
YRN1 and HEAHIO proteins. ESTs gb|T21954, gb|T04283,
gb|Z37609, gb|N37366, gb|R90704, gb|F15500 and gb|F14353
come from this gene. [Arabidopsis thaliana]
gi|11094299|dbj|BAB17665.1| Glyoxalase I homolog
[Arabidopsis thaliana]
gi|16226213|gb|AAL16104.1|AF428272_1 At1g11840/F12F1_32
[Arabidopsis thaliana] gi|18377845|gb|AAL67109.1|
At1g11840/F12F1_32 [Arabidopsis thaliana]
gi|20453275|gb|AAM19876.1| At1g11840/F12F1_32
[Arabidopsis thaliana] gi|21593459|gb|AAM65426.1|
lactoylglutathione lyase-like protein [Arabidopsis
thaliana]
Length = 283
Score = 286 bits (733), Expect = 1e-76
Identities = 134/166 (80%), Positives = 151/166 (90%)
Frame = +1
Query: 73 ADLWEWPKKDNRRLLHVVYRVGDLERTIKFYTEALGMKLLRQRDIPEEKYANAFLGFGPE 252
+DL EWPKKDNRR LHVVYRVGDL+RTI+FYTE GMKLLR+RDIPEEKY+NAFLGFGPE
Sbjct: 5 SDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLGFGPE 64
Query: 253 ETHFVVELTYNYGVSSYDIGNGFGHFAIATQDIHKLVEHIRGKGGNITREPGPVQGGTTV 432
++FVVELTYNYGVSSYDIG GFGHFAI+TQD+ KLVE++R KGGN+TREPGPV+GG +V
Sbjct: 65 TSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSV 124
Query: 433 IAFVKDPDGYTFGLIQRPTVHDPFCQIMLRVGDLERAINFYEKALG 570
IAFVKDPDGYTF LIQR +PFCQ+MLRVGDL+RAI FYEKALG
Sbjct: 125 IAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALG 170
Score = 108 bits (270), Expect = 5e-23
Identities = 59/124 (47%), Positives = 80/124 (63%), Gaps = 5/124 (4%)
Frame = +1
Query: 121 VVYRVGDLERTIKFYTEALGMKLLRQRDIPEEKYANAFLGFGPEETHFVVELTYNYGVSS 300
V+ RVGDL+R IKFY +ALGM+LLR+ + PE KY +G+ E V+ELTYNY V+
Sbjct: 151 VMLRVGDLDRAIKFYEKALGMRLLRKIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTE 210
Query: 301 YDIGNGFGHFAIATQDIHKLVEHIR----GKGGNITREPGPVQG-GTTVIAFVKDPDGYT 465
Y GN + AI T D++K E I+ GG ITRE GP+ G GT +++F+ DPDG+
Sbjct: 211 YTKGNAYAQIAIGTDDVYKSGEVIKIVNQELGGKITREAGPLPGLGTKIVSFL-DPDGWK 269
Query: 466 FGLI 477
L+
Sbjct: 270 TVLV 273
>gb|AAL07227.1| putative lactoylglutathione lyase [Arabidopsis thaliana]
Length = 283
Score = 283 bits (725), Expect = 8e-76
Identities = 133/166 (80%), Positives = 150/166 (90%)
Frame = +1
Query: 73 ADLWEWPKKDNRRLLHVVYRVGDLERTIKFYTEALGMKLLRQRDIPEEKYANAFLGFGPE 252
+DL EWPKKDNRR LHVVYRVGDL+RTI+FYTE GMKLL +RDIPEEKY+NAFLGFGPE
Sbjct: 5 SDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLWKRDIPEEKYSNAFLGFGPE 64
Query: 253 ETHFVVELTYNYGVSSYDIGNGFGHFAIATQDIHKLVEHIRGKGGNITREPGPVQGGTTV 432
++FVVELTYNYGVSSYDIG GFGHFAI+TQD+ KLVE++R KGGN+TREPGPV+GG +V
Sbjct: 65 TSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKGGGSV 124
Query: 433 IAFVKDPDGYTFGLIQRPTVHDPFCQIMLRVGDLERAINFYEKALG 570
IAFVKDPDGYTF LIQR +PFCQ+MLRVGDL+RAI FYEKALG
Sbjct: 125 IAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALG 170
Score = 108 bits (270), Expect = 5e-23
Identities = 59/124 (47%), Positives = 80/124 (63%), Gaps = 5/124 (4%)
Frame = +1
Query: 121 VVYRVGDLERTIKFYTEALGMKLLRQRDIPEEKYANAFLGFGPEETHFVVELTYNYGVSS 300
V+ RVGDL+R IKFY +ALGM+LLR+ + PE KY +G+ E V+ELTYNY V+
Sbjct: 151 VMLRVGDLDRAIKFYEKALGMRLLRKIERPEYKYTIGMMGYAEEYESIVLELTYNYDVTE 210
Query: 301 YDIGNGFGHFAIATQDIHKLVEHIR----GKGGNITREPGPVQG-GTTVIAFVKDPDGYT 465
Y GN + AI T D++K E I+ GG ITRE GP+ G GT +++F+ DPDG+
Sbjct: 211 YTKGNAYAQIAIGTDDVYKSGEVIKIVNQELGGKITREAGPLPGLGTKIVSFL-DPDGWK 269
Query: 466 FGLI 477
L+
Sbjct: 270 TVLV 273
>emb|CAB09799.1| hypothetical protein [Citrus x paradisi]
Length = 291
Score = 281 bits (718), Expect = 5e-75
Identities = 130/166 (78%), Positives = 148/166 (88%)
Frame = +1
Query: 73 ADLWEWPKKDNRRLLHVVYRVGDLERTIKFYTEALGMKLLRQRDIPEEKYANAFLGFGPE 252
A+L EWPKKD RR LH VYRVGDL+RTIKFYTE GMKLLR+RD+PEEKY+NAFLGFGPE
Sbjct: 11 AELLEWPKKDKRRFLHAVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPE 70
Query: 253 ETHFVVELTYNYGVSSYDIGNGFGHFAIATQDIHKLVEHIRGKGGNITREPGPVQGGTTV 432
++HFVVELTYNYGV+SYDIG GFGHFAIAT+D++KLVE+IR KGGN+TREPGP++GGTT
Sbjct: 71 QSHFVVELTYNYGVTSYDIGTGFGHFAIATEDVYKLVENIRAKGGNVTREPGPLKGGTTH 130
Query: 433 IAFVKDPDGYTFGLIQRPTVHDPFCQIMLRVGDLERAINFYEKALG 570
IAFVKDPDGY F LIQR +P CQ+MLRVGDL R+I FYEKALG
Sbjct: 131 IAFVKDPDGYIFELIQRGPTPEPLCQVMLRVGDLGRSIKFYEKALG 176
Score = 115 bits (287), Expect = 5e-25
Identities = 62/126 (49%), Positives = 79/126 (62%), Gaps = 4/126 (3%)
Frame = +1
Query: 112 LLHVVYRVGDLERTIKFYTEALGMKLLRQRDIPEEKYANAFLGFGPEETHFVVELTYNYG 291
L V+ RVGDL R+IKFY +ALGMKLLR D PE KY A LG+ E+ V+ELTYNYG
Sbjct: 154 LCQVMLRVGDLGRSIKFYEKALGMKLLRTVDKPEYKYTLAMLGYAEEDQTTVLELTYNYG 213
Query: 292 VSSYDIGNGFGHFAIATQDIHKLVEHI----RGKGGNITREPGPVQGGTTVIAFVKDPDG 459
V+ Y GN + AI+T D++K E + + GG ITR+PG + G T I DPDG
Sbjct: 214 VTEYTKGNAYAQVAISTDDVYKSAEVVNLVTQELGGKITRQPGSIPGLNTKITSFVDPDG 273
Query: 460 YTFGLI 477
+ L+
Sbjct: 274 WKTVLV 279
>sp|Q39366|LGUL_BRAOL Putative lactoylglutathione lyase (Methylglyoxalase)
(Aldoketomutase) (Glyoxalase I) (Glx I) (Ketone-aldehyde
mutase) (S-D-lactoylglutathione methylglyoxal lyase)
gi|7488555|pir||T14440 hypothetical protein - wild
cabbage gi|1469221|emb|CAA99248.1| protein similar to
bacterial YRN1 and HEAHIO proteins [Brassica oleracea]
Length = 282
Score = 276 bits (705), Expect = 2e-73
Identities = 128/166 (77%), Positives = 147/166 (88%)
Frame = +1
Query: 73 ADLWEWPKKDNRRLLHVVYRVGDLERTIKFYTEALGMKLLRQRDIPEEKYANAFLGFGPE 252
ADL EWPKKD RR LHVVYRVGDL+RTI+FYTE GMK+LR+RD+PEEKY+NAFLGFGPE
Sbjct: 5 ADLVEWPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLGFGPE 64
Query: 253 ETHFVVELTYNYGVSSYDIGNGFGHFAIATQDIHKLVEHIRGKGGNITREPGPVQGGTTV 432
++FVVELTYNYGVSSYDIG GFGHFAI+TQD+ K+VE +R KGGN+TREPGPV+GG +V
Sbjct: 65 TSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKMVEAVRAKGGNVTREPGPVKGGGSV 124
Query: 433 IAFVKDPDGYTFGLIQRPTVHDPFCQIMLRVGDLERAINFYEKALG 570
IAFVKDPDGYTF LIQR +P CQ+MLRVGDL+RA+ F EKALG
Sbjct: 125 IAFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALG 170
Score = 100 bits (248), Expect = 2e-20
Identities = 55/122 (45%), Positives = 77/122 (63%), Gaps = 5/122 (4%)
Frame = +1
Query: 112 LLHVVYRVGDLERTIKFYTEALGMKLLRQRDIPEEKYANAFLGFGPEETHFVVELTYNYG 291
L V+ RVGDL+R +KF +ALGM+LLR+ + PE +G+ E V+ELTYNYG
Sbjct: 148 LCQVMLRVGDLDRAVKFMEKALGMRLLRRIERPEYNTIG-MMGYAEEYESIVLELTYNYG 206
Query: 292 VSSYDIGNGFGHFAIATQDIHKLVEHIR----GKGGNITREPGPVQG-GTTVIAFVKDPD 456
V+ Y GN + AI T D++K E ++ GG ITRE GP+ G GT +++F+ DPD
Sbjct: 207 VTEYTKGNAYAQIAIGTDDVYKSAEVVKIVNQELGGKITREAGPLPGLGTKIVSFL-DPD 265
Query: 457 GY 462
G+
Sbjct: 266 GW 267
>emb|CAB50787.2| putative glyoxalase I [Triticum aestivum]
Length = 284
Score = 266 bits (681), Expect = 1e-70
Identities = 122/165 (73%), Positives = 142/165 (85%)
Frame = +1
Query: 76 DLWEWPKKDNRRLLHVVYRVGDLERTIKFYTEALGMKLLRQRDIPEEKYANAFLGFGPEE 255
+ + W KKDNRRLLHVVYRVGD++RTIKFYTE LGMKLLR+RDIPEEKY NAFLG+GPEE
Sbjct: 11 EAFTWAKKDNRRLLHVVYRVGDIDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPEE 70
Query: 256 THFVVELTYNYGVSSYDIGNGFGHFAIATQDIHKLVEHIRGKGGNITREPGPVQGGTTVI 435
T+F +ELTYNYGV SYDIG GFGHF IAT D+ K VE IR KGG +TREPGPV+GG TVI
Sbjct: 71 TNFAIELTYNYGVDSYDIGAGFGHFGIATDDVAKTVELIRAKGGKVTREPGPVKGGKTVI 130
Query: 436 AFVKDPDGYTFGLIQRPTVHDPFCQIMLRVGDLERAINFYEKALG 570
AF++DPDGY F +++RP +P CQ+MLRVGDL+RAI+FYEKA G
Sbjct: 131 AFIEDPDGYKFEILERPGTPEPLCQVMLRVGDLDRAISFYEKACG 175
Score = 129 bits (323), Expect = 3e-29
Identities = 60/117 (51%), Positives = 80/117 (68%)
Frame = +1
Query: 112 LLHVVYRVGDLERTIKFYTEALGMKLLRQRDIPEEKYANAFLGFGPEETHFVVELTYNYG 291
L V+ RVGDL+R I FY +A GMKLLR+RD PE KY A +G+GPE+ + V+ELTYNYG
Sbjct: 153 LCQVMLRVGDLDRAISFYEKACGMKLLRKRDNPEYKYTIAMMGYGPEDQNAVLELTYNYG 212
Query: 292 VSSYDIGNGFGHFAIATQDIHKLVEHIRGKGGNITREPGPVQGGTTVIAFVKDPDGY 462
V+ YD GN + AI T D++K E ++ GG + RE GP+ G T I + DP+G+
Sbjct: 213 VTEYDKGNAYAQIAIGTDDVYKTAEVVKLSGGKVVREAGPLPGLGTKITAILDPBGW 269
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 514,147,051
Number of Sequences: 1393205
Number of extensions: 11710072
Number of successful extensions: 26902
Number of sequences better than 10.0: 256
Number of HSP's better than 10.0 without gapping: 25668
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 26664
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20956655091
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
