>KMC004948A_C02 KMC004948A_c02
gaaatttAGGAAGGAGTGTATTCCAAATAACAATTTTAAGTAAGCAACAATTTCTTAAGT
AATTTACAATTGAGTCAATAAAAATATACACTTCAACATAATCAAATTAAAATTAACTAA
TATAGTTAACAGTACAAATAAATTCTCCCATGCGCTGAACTCCAACCATTTTCTAAAACT
ATTTTCTTCCTATAGTTTTTGCTTGAATTCTCTTCAAATAAGAACCTCTTCCTTTGAAAC
ACACATTGTCTCGTCATGATTTATGACTTCAAAATACTGGTTCTTTGTATGCTATTGTAA
CAACCCTTCAGGTCCGTGGAGTTAAGGAATGTAGCAAGGTCTAAATGAACAATCAAACTG
GTCACAGTCCATGACATTCATATAATGTAGCATAGATTCACATTTATGGTGTGCAAATAA
CTTGACAGCACCACGACTTTCAATTTCAGTGACAAACTGAACCTTATATGGAAAATTAGC
ATAGTTTCACTTACCCTTCCATAACGCAACGTGCTGCTTGACCTCCCCTTCCGCACCTTC
AAGATTATGATACTGCTTTGGACATGACGTTGTTCTCTTTTGGAATTATCTCTTTTCATC
ATCAATCAATACGATC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004948A_C02 KMC004948A_c02
         (616 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_197735.1| putative protein; protein id: At5g23440.1 [Arab...    62  8e-09
ref|NP_568195.1| Expressed protein; protein id: At5g08410.1, sup...    60  2e-08
emb|CAC08341.1| lipoic acid synthase-like protein [Arabidopsis t...    60  2e-08
sp|P80680|FTRV_MAIZE FERREDOXIN-THIOREDOXIN REDUCTASE, VARIABLE ...    50  3e-05
pir||JT0703 ferredoxin-thioredoxin reductase (EC 1.18.-.-) varia...    49  4e-05

>ref|NP_197735.1| putative protein; protein id: At5g23440.1 [Arabidopsis thaliana]
           gi|9759082|dbj|BAB09560.1| contains similarity to
           ferredoxin-thioredoxin reductase variable
           chain~gene_id:K19M13.7 [Arabidopsis thaliana]
          Length = 182

 Score = 61.6 bits (148), Expect = 8e-09
 Identities = 32/65 (49%), Positives = 41/65 (62%), Gaps = 7/65 (10%)
 Frame = -2

Query: 576 RTTSCPKQYH-------NLEGAEGEVKQHVALWKGK*NYANFPYKVQFVTEIESRGAVKL 418
           R T+  K YH       +LEG EG++K +VA+WKGK   AN PYK++F  EIE RG VK 
Sbjct: 109 RVTAPLKVYHVNRVPEVDLEGMEGKLKDYVAVWKGKRISANLPYKIEFFKEIEGRGLVKF 168

Query: 417 FAHHK 403
            +H K
Sbjct: 169 VSHLK 173

>ref|NP_568195.1| Expressed protein; protein id: At5g08410.1, supported by cDNA:
           31366. [Arabidopsis thaliana] gi|21592688|gb|AAM64637.1|
           unknown [Arabidopsis thaliana]
           gi|26449733|dbj|BAC41990.1| putative lipoic acid
           synthase [Arabidopsis thaliana]
           gi|28372908|gb|AAO39936.1| At5g08410 [Arabidopsis
           thaliana]
          Length = 184

 Score = 60.5 bits (145), Expect = 2e-08
 Identities = 34/65 (52%), Positives = 37/65 (56%), Gaps = 7/65 (10%)
 Frame = -2

Query: 576 RTTSCPKQYH-------NLEGAEGEVKQHVALWKGK*NYANFPYKVQFVTEIESRGAVKL 418
           R T   K YH        L G EG +K +V LWKGK   AN P+KVQFV EIE RG VK 
Sbjct: 111 RVTVPLKVYHVVRVPEVELMGMEGFIKDYVVLWKGKKISANLPFKVQFVKEIEGRGPVKF 170

Query: 417 FAHHK 403
           F H K
Sbjct: 171 FTHLK 175

>emb|CAC08341.1| lipoic acid synthase-like protein [Arabidopsis thaliana]
          Length = 477

 Score = 60.5 bits (145), Expect = 2e-08
 Identities = 34/65 (52%), Positives = 37/65 (56%), Gaps = 7/65 (10%)
 Frame = -2

Query: 576 RTTSCPKQYH-------NLEGAEGEVKQHVALWKGK*NYANFPYKVQFVTEIESRGAVKL 418
           R T   K YH        L G EG +K +V LWKGK   AN P+KVQFV EIE RG VK 
Sbjct: 404 RVTVPLKVYHVVRVPEVELMGMEGFIKDYVVLWKGKKISANLPFKVQFVKEIEGRGPVKF 463

Query: 417 FAHHK 403
           F H K
Sbjct: 464 FTHLK 468

>sp|P80680|FTRV_MAIZE FERREDOXIN-THIOREDOXIN REDUCTASE, VARIABLE CHAIN (FTR-V)
           (FERREDOXIN-THIOREDOXIN REDUCTASE SUBUNIT A) (FTR-A)
          Length = 97

 Score = 49.7 bits (117), Expect = 3e-05
 Identities = 27/64 (42%), Positives = 39/64 (60%), Gaps = 8/64 (12%)
 Frame = -2

Query: 576 RTTSCPKQYH-------NLEGAEGEVKQHVALWKGK*NYANFPYKVQFVTEIESR-GAVK 421
           R T+  + YH       +++G EG VKQ+V +WKGK   ANFP+KV+F   +E +   V+
Sbjct: 23  RVTAPLRVYHVLKAPDLDIQGMEGVVKQYVCVWKGKRVTANFPFKVEFELAVEGQPKPVR 82

Query: 420 LFAH 409
            FAH
Sbjct: 83  FFAH 86

>pir||JT0703 ferredoxin-thioredoxin reductase (EC 1.18.-.-) variable chain -
           maize
          Length = 97

 Score = 49.3 bits (116), Expect = 4e-05
 Identities = 27/64 (42%), Positives = 39/64 (60%), Gaps = 8/64 (12%)
 Frame = -2

Query: 576 RTTSCPKQYH-------NLEGAEGEVKQHVALWKGK*NYANFPYKVQFVTEIESR-GAVK 421
           R T+  + YH       +++G EG VKQ+V +WKGK   ANFP+KV+F   +E +   V+
Sbjct: 23  RVTAPLRVYHVLKAPDLDIQGMEGVVKQYVRVWKGKRVTANFPFKVEFELAVEGQPKPVR 82

Query: 420 LFAH 409
            FAH
Sbjct: 83  FFAH 86

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 464,646,113
Number of Sequences: 1393205
Number of extensions: 9028458
Number of successful extensions: 17153
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 16586
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 17143
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 24854530794
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MRL034c06_f BP085389 1 507 2 SPDL077h10_f BP056814 8 296 3 SPDL063b04_f BP055892 20 554 4 MPDL008c01_f AV776909 24 616 5 SPDL047c12_f BP054938 38 473

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