Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004895A_C01 KMC004895A_c01
(631 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_195308.1| cytoplasmatic aconitate hydratase (citrate hydr... 162 6e-75
emb|CAA58046.1| aconitase [Arabidopsis thaliana] 162 2e-74
gb|AAM97080.1| cytoplasmic aconitate hydratase [Arabidopsis thal... 150 1e-70
sp|P49608|ACOC_CUCMA Aconitate hydratase, cytoplasmic (Citrate h... 152 1e-70
gb|AAG28426.1|AF194945_1 cytosolic aconitase [Nicotiana tabacum] 151 2e-70
>ref|NP_195308.1| cytoplasmatic aconitate hydratase (citrate
hydro-lyase)(aconitase)(EC 4.2.1.3); protein id:
At4g35830.1, supported by cDNA: gi_17065391 [Arabidopsis
thaliana] gi|13124706|sp|Q42560|ACOC_ARATH Aconitate
hydratase, cytoplasmic (Citrate hydro-lyase) (Aconitase)
gi|7437036|pir||T04693 aconitate hydratase (EC 4.2.1.3),
cytosolic - Arabidopsis thaliana
gi|3805849|emb|CAA21469.1| cytoplasmatic aconitate
hydratase (citrate hydro-lyase)(aconitase)(EC 4.2.1.3)
[Arabidopsis thaliana] gi|7270535|emb|CAB81492.1|
cytoplasmatic aconitate hydratase (citrate
hydro-lyase)(aconitase)(EC 4.2.1.3) [Arabidopsis
thaliana] gi|17065392|gb|AAL32850.1| Unknown protein
[Arabidopsis thaliana]
Length = 898
Score = 162 bits (411), Expect(3) = 6e-75
Identities = 78/84 (92%), Positives = 82/84 (96%)
Frame = +3
Query: 201 ALNDPRIEALPYSIRILLESAIRNCDEFQVKSNDVEKIIDWKNTAPKQVEIPFKPARVLL 380
ALNDPRI+ LPYSIRILLESAIRNCDEFQVKS DVEKI+DW+NT+PKQVEIPFKPARVLL
Sbjct: 30 ALNDPRIDKLPYSIRILLESAIRNCDEFQVKSKDVEKILDWENTSPKQVEIPFKPARVLL 89
Query: 381 QDFTGVPAVVDLACMRDAMNNLGG 452
QDFTGVPAVVDLACMRDAMNNLGG
Sbjct: 90 QDFTGVPAVVDLACMRDAMNNLGG 113
Score = 117 bits (293), Expect(3) = 6e-75
Identities = 57/59 (96%), Positives = 58/59 (97%)
Frame = +1
Query: 454 DSNKINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFGFLKWGSNAFNNML 630
DSNKINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERF FLKWGSNAF+NML
Sbjct: 114 DSNKINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFAFLKWGSNAFHNML 172
Score = 44.3 bits (103), Expect(3) = 6e-75
Identities = 21/29 (72%), Positives = 25/29 (85%)
Frame = +2
Query: 110 MATDHPFQSILKTLEKPGGGGEFGKYYSL 196
MA+++PF+SILK LEKP GGEFG YYSL
Sbjct: 1 MASENPFRSILKALEKP-DGGEFGNYYSL 28
>emb|CAA58046.1| aconitase [Arabidopsis thaliana]
Length = 919
Score = 162 bits (411), Expect(3) = 2e-74
Identities = 78/84 (92%), Positives = 82/84 (96%)
Frame = +3
Query: 201 ALNDPRIEALPYSIRILLESAIRNCDEFQVKSNDVEKIIDWKNTAPKQVEIPFKPARVLL 380
ALNDPRI+ LPYSIRILLESAIRNCDEFQVKS DVEKI+DW+NT+PKQVEIPFKPARVLL
Sbjct: 52 ALNDPRIDKLPYSIRILLESAIRNCDEFQVKSKDVEKILDWENTSPKQVEIPFKPARVLL 111
Query: 381 QDFTGVPAVVDLACMRDAMNNLGG 452
QDFTGVPAVVDLACMRDAMNNLGG
Sbjct: 112 QDFTGVPAVVDLACMRDAMNNLGG 135
Score = 115 bits (287), Expect(3) = 2e-74
Identities = 56/59 (94%), Positives = 58/59 (97%)
Frame = +1
Query: 454 DSNKINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFGFLKWGSNAFNNML 630
DSNKINPLVPVDLVID+SVQVDVARSENAVQANMELEFQRNKERF FLKWGSNAF+NML
Sbjct: 136 DSNKINPLVPVDLVIDYSVQVDVARSENAVQANMELEFQRNKERFAFLKWGSNAFHNML 194
Score = 44.7 bits (104), Expect(3) = 2e-74
Identities = 21/30 (70%), Positives = 26/30 (86%)
Frame = +2
Query: 107 TMATDHPFQSILKTLEKPGGGGEFGKYYSL 196
+MA+++PF+SILK LEKP GGEFG YYSL
Sbjct: 22 SMASENPFRSILKALEKP-DGGEFGNYYSL 50
>gb|AAM97080.1| cytoplasmic aconitate hydratase [Arabidopsis thaliana]
Length = 990
Score = 150 bits (379), Expect(3) = 1e-70
Identities = 72/83 (86%), Positives = 76/83 (90%)
Frame = +3
Query: 201 ALNDPRIEALPYSIRILLESAIRNCDEFQVKSNDVEKIIDWKNTAPKQVEIPFKPARVLL 380
ALNDPR++ LPYSIRILLESAIRNCD FQV DVEKIIDW+ T+PKQVEIPFKPARVLL
Sbjct: 122 ALNDPRVDKLPYSIRILLESAIRNCDNFQVTKEDVEKIIDWEKTSPKQVEIPFKPARVLL 181
Query: 381 QDFTGVPAVVDLACMRDAMNNLG 449
QDFTGVPAVVDLACMRDAMN LG
Sbjct: 182 QDFTGVPAVVDLACMRDAMNKLG 204
Score = 114 bits (286), Expect(3) = 1e-70
Identities = 56/59 (94%), Positives = 56/59 (94%)
Frame = +1
Query: 454 DSNKINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFGFLKWGSNAFNNML 630
DSNKINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERF FLKWGS AF NML
Sbjct: 206 DSNKINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFAFLKWGSTAFQNML 264
Score = 44.7 bits (104), Expect(3) = 1e-70
Identities = 20/30 (66%), Positives = 25/30 (82%)
Frame = +2
Query: 107 TMATDHPFQSILKTLEKPGGGGEFGKYYSL 196
+MA++HPF+ I TL KP GGGEFGK+YSL
Sbjct: 92 SMASEHPFKGIFTTLPKP-GGGEFGKFYSL 120
>sp|P49608|ACOC_CUCMA Aconitate hydratase, cytoplasmic (Citrate hydro-lyase) (Aconitase)
gi|7437043|pir||T10101 aconitate hydratase (EC 4.2.1.3)
- cucurbit gi|868003|dbj|BAA06108.1| aconitase
[Cucurbita cv. Kurokawa Amakuri]
Length = 898
Score = 152 bits (385), Expect(3) = 1e-70
Identities = 73/83 (87%), Positives = 78/83 (93%)
Frame = +3
Query: 201 ALNDPRIEALPYSIRILLESAIRNCDEFQVKSNDVEKIIDWKNTAPKQVEIPFKPARVLL 380
+LNDPRI+ LPYSIRILLESAIRNCD FQVK DVEKIIDW+N++PKQVEIPFKPARVLL
Sbjct: 30 SLNDPRIDRLPYSIRILLESAIRNCDNFQVKKEDVEKIIDWENSSPKQVEIPFKPARVLL 89
Query: 381 QDFTGVPAVVDLACMRDAMNNLG 449
QDFTGVPAVVDLACMRDAMN LG
Sbjct: 90 QDFTGVPAVVDLACMRDAMNKLG 112
Score = 117 bits (292), Expect(3) = 1e-70
Identities = 57/59 (96%), Positives = 57/59 (96%)
Frame = +1
Query: 454 DSNKINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFGFLKWGSNAFNNML 630
DSNKINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERF FLKWGSNAF NML
Sbjct: 114 DSNKINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFAFLKWGSNAFQNML 172
Score = 40.0 bits (92), Expect(3) = 1e-70
Identities = 19/29 (65%), Positives = 23/29 (78%)
Frame = +2
Query: 110 MATDHPFQSILKTLEKPGGGGEFGKYYSL 196
MA ++PF+ L +L KPGGG EFGKYYSL
Sbjct: 1 MAAENPFKENLTSLPKPGGG-EFGKYYSL 28
>gb|AAG28426.1|AF194945_1 cytosolic aconitase [Nicotiana tabacum]
Length = 898
Score = 151 bits (382), Expect(3) = 2e-70
Identities = 74/83 (89%), Positives = 76/83 (91%)
Frame = +3
Query: 201 ALNDPRIEALPYSIRILLESAIRNCDEFQVKSNDVEKIIDWKNTAPKQVEIPFKPARVLL 380
ALNDPRI+ LPYS RILLESAIRNCD FQVK DVEKIIDW+NTAPK VEIPFKPARVLL
Sbjct: 30 ALNDPRIDKLPYSSRILLESAIRNCDNFQVKKEDVEKIIDWENTAPKLVEIPFKPARVLL 89
Query: 381 QDFTGVPAVVDLACMRDAMNNLG 449
QDFTGVPAVVDLACMRDAMN LG
Sbjct: 90 QDFTGVPAVVDLACMRDAMNKLG 112
Score = 115 bits (288), Expect(3) = 2e-70
Identities = 56/59 (94%), Positives = 58/59 (97%)
Frame = +1
Query: 454 DSNKINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFGFLKWGSNAFNNML 630
DS+KINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERF FLKWGSNAF+NML
Sbjct: 114 DSDKINPLVPVDLVIDHSVQVDVARSENAVQANMELEFQRNKERFAFLKWGSNAFHNML 172
Score = 42.4 bits (98), Expect(3) = 2e-70
Identities = 20/29 (68%), Positives = 23/29 (78%)
Frame = +2
Query: 110 MATDHPFQSILKTLEKPGGGGEFGKYYSL 196
MA ++PF+ IL L KP GGGEFGKYYSL
Sbjct: 1 MAAENPFKGILTVLPKP-GGGEFGKYYSL 28
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 541,446,081
Number of Sequences: 1393205
Number of extensions: 11416388
Number of successful extensions: 40178
Number of sequences better than 10.0: 166
Number of HSP's better than 10.0 without gapping: 37414
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 40008
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 25870486187
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
