Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004889A_C01 KMC004889A_c01
(578 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_680757.1| similar to polygalacturonase, putative; protein... 55 6e-15
pir||T05347 hypothetical protein F8B4.70 - Arabidopsis thaliana ... 55 3e-14
ref|NP_194964.2| polygalacturonase, putative; protein id: At4g32... 60 6e-14
pir||T05348 hypothetical protein F8B4.80 - Arabidopsis thaliana ... 60 6e-14
gb|AAD17250.1| polygalacturonase [Lycopersicon esculentum] 57 4e-12
>ref|NP_680757.1| similar to polygalacturonase, putative; protein id: At4g32375.1
[Arabidopsis thaliana]
Length = 486
Score = 55.5 bits (132), Expect(2) = 6e-15
Identities = 26/55 (47%), Positives = 38/55 (68%), Gaps = 1/55 (1%)
Frame = +1
Query: 343 ALKVTDVTFRNIHGTCTNEHAVVLDCAKI-GCDNIKLQQINITSIDLDKPASAIC 504
A+KV++VTFR GTC N+ A+ LDC ++ GC +I ++ INITS +P +A C
Sbjct: 287 AVKVSNVTFRYFTGTCANDIAIKLDCDEVTGCKDIVMEHINITSSSTKRPLTAYC 341
Score = 46.6 bits (109), Expect(2) = 6e-15
Identities = 19/32 (59%), Positives = 24/32 (74%)
Frame = +2
Query: 134 TMKGGKGYAKAIIYENIIVNQTNYPVYIDQHY 229
T++GG G AK I+YENI + T YP+ IDQHY
Sbjct: 242 TVRGGLGVAKNILYENITLTDTKYPIIIDQHY 273
>pir||T05347 hypothetical protein F8B4.70 - Arabidopsis thaliana
gi|4049339|emb|CAA22564.1| putative protein [Arabidopsis
thaliana] gi|7270141|emb|CAB79954.1| putative protein
[Arabidopsis thaliana]
Length = 503
Score = 55.5 bits (132), Expect(2) = 3e-14
Identities = 26/55 (47%), Positives = 38/55 (68%), Gaps = 1/55 (1%)
Frame = +1
Query: 343 ALKVTDVTFRNIHGTCTNEHAVVLDCAKI-GCDNIKLQQINITSIDLDKPASAIC 504
A+KV++VTFR GTC N+ A+ LDC ++ GC +I ++ INITS +P +A C
Sbjct: 304 AVKVSNVTFRYFTGTCANDIAIKLDCDEVTGCKDIVMEHINITSSSTKRPLTAYC 358
Score = 46.2 bits (108), Expect(2) = 1e-10
Identities = 28/78 (35%), Positives = 41/78 (51%), Gaps = 1/78 (1%)
Frame = +1
Query: 289 FGDNLHHSFPLLFWLQKEALKVTDVTFRNIHGTCTNEHAVVLDCAKI-GCDNIKLQQINI 465
F + H F F L++ +KV ++TFR GT ++E + LDC + C NI ++ INI
Sbjct: 95 FDNKKKHYFDKSF-LKESGVKVDNITFRYFEGTSSSEIPIKLDCDETENCHNITMEHINI 153
Query: 466 TSIDLDKPASAICHNVHG 519
TS K +A C G
Sbjct: 154 TSPTPGKNLTAYCKFADG 171
Score = 44.3 bits (103), Expect(2) = 3e-14
Identities = 18/31 (58%), Positives = 23/31 (74%)
Frame = +2
Query: 137 MKGGKGYAKAIIYENIIVNQTNYPVYIDQHY 229
++GG G AK I+YENI + T YP+ IDQHY
Sbjct: 260 IQGGLGVAKNILYENITLTDTKYPIIIDQHY 290
Score = 41.2 bits (95), Expect(2) = 1e-10
Identities = 17/35 (48%), Positives = 23/35 (65%)
Frame = +2
Query: 143 GGKGYAKAIIYENIIVNQTNYPVYIDQHYMRTPEK 247
GG+G AK I+YENI + YP+ I+QHY +K
Sbjct: 66 GGRGLAKNILYENITLIDAGYPIIINQHYFDNKKK 100
>ref|NP_194964.2| polygalacturonase, putative; protein id: At4g32380.1 [Arabidopsis
thaliana]
Length = 354
Score = 60.1 bits (144), Expect(2) = 6e-14
Identities = 33/75 (44%), Positives = 43/75 (57%), Gaps = 1/75 (1%)
Frame = +1
Query: 343 ALKVTDVTFRNIHGTCTNEHAVVLDC-AKIGCDNIKLQQINITSIDLDKPASAICHNVHG 519
A+KV+DVTFR+ GTC A+ LDC GCDNI ++QINI S P ++ C H
Sbjct: 225 AVKVSDVTFRSFTGTCAAPIAIKLDCDPNTGCDNIVMEQINIASSSPKTPLTSYCKFAHV 284
Query: 520 TATDVISPRVTCLHH 564
+ V P +TC H
Sbjct: 285 VSRFVSIP-ITCSFH 298
Score = 38.5 bits (88), Expect(2) = 6e-14
Identities = 15/32 (46%), Positives = 22/32 (67%)
Frame = +2
Query: 134 TMKGGKGYAKAIIYENIIVNQTNYPVYIDQHY 229
T GG+G+ K I+YE+I + N+P+ IDQ Y
Sbjct: 179 TWPGGQGFVKNILYEDITLINANFPIIIDQQY 210
>pir||T05348 hypothetical protein F8B4.80 - Arabidopsis thaliana
gi|4049340|emb|CAA22565.1| putative protein [Arabidopsis
thaliana] gi|7270142|emb|CAB79955.1| putative protein
[Arabidopsis thaliana]
Length = 312
Score = 60.1 bits (144), Expect(2) = 6e-14
Identities = 33/75 (44%), Positives = 43/75 (57%), Gaps = 1/75 (1%)
Frame = +1
Query: 343 ALKVTDVTFRNIHGTCTNEHAVVLDC-AKIGCDNIKLQQINITSIDLDKPASAICHNVHG 519
A+KV+DVTFR+ GTC A+ LDC GCDNI ++QINI S P ++ C H
Sbjct: 183 AVKVSDVTFRSFTGTCAAPIAIKLDCDPNTGCDNIVMEQINIASSSPKTPLTSYCKFAHV 242
Query: 520 TATDVISPRVTCLHH 564
+ V P +TC H
Sbjct: 243 VSRFVSIP-ITCSFH 256
Score = 38.5 bits (88), Expect(2) = 6e-14
Identities = 15/32 (46%), Positives = 22/32 (67%)
Frame = +2
Query: 134 TMKGGKGYAKAIIYENIIVNQTNYPVYIDQHY 229
T GG+G+ K I+YE+I + N+P+ IDQ Y
Sbjct: 137 TWPGGQGFVKNILYEDITLINANFPIIIDQQY 168
>gb|AAD17250.1| polygalacturonase [Lycopersicon esculentum]
Length = 367
Score = 56.6 bits (135), Expect(2) = 4e-12
Identities = 30/76 (39%), Positives = 49/76 (64%), Gaps = 1/76 (1%)
Frame = +1
Query: 334 QKEALKVTDVTFRNIHGTCTNEHAVVLDCAK-IGCDNIKLQQINITSIDLDKPASAICHN 510
Q A+KV++VT++ I+GT +++ A+ ++C+ C N++L+ I ITS++ K A C+N
Sbjct: 291 QVSAVKVSNVTYKKIYGTTSSKLAIKMNCSNSTACTNVELENIYITSVEPGKKIFATCNN 350
Query: 511 VHGTATDVISPRVTCL 558
V G A SP V CL
Sbjct: 351 VKGKAFS-NSPHVFCL 365
Score = 35.8 bits (81), Expect(2) = 4e-12
Identities = 15/32 (46%), Positives = 21/32 (64%)
Frame = +2
Query: 134 TMKGGKGYAKAIIYENIIVNQTNYPVYIDQHY 229
T +GG GYA+ I +ENI + P+ IDQ+Y
Sbjct: 250 TWQGGSGYARFIHFENINLKNVENPIIIDQNY 281
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 459,483,405
Number of Sequences: 1393205
Number of extensions: 9412422
Number of successful extensions: 20698
Number of sequences better than 10.0: 171
Number of HSP's better than 10.0 without gapping: 19964
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 20639
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 21426319650
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
