>KMC004850A_C01 KMC004850A_c01
agcaggataagaagtctttactgtCAGAAAAAGAACATATTTATACAAAACAAATTAGGC
CATTCTAATATGTTCCCAAATAAAAGATATACATCACGATATAGCTAAGATTGTACTACA
GAGATCTTTGATACAAAACAACCAACACTCTCTAAAGTTTCTTCCTAAGAGACATTAAAA
TATCTGAGGTCTCACACAATGTTGTGACAAACTAAAAACTACACAATCATCCCAAAAATT
ATGATCACACTGATATCCTTTTTTACCTGCTTGTTTGGCTTGAATGTATTTTGCTCACAA
TGTTCTTCTGCTACATGACCAGTTTCAGCTTTTTATGGTTGGTGTGACCGTTTCAAAAGC
TTCCACTAGCAGCTCCACTTTTCTTCTCCTAGCTGGGGTTAGTTTCGAAACAACCTGTCG
AAGCGCATAATCAAGCATCCATTCTTCTTGGCCTTTTCTTGCTTCCATGTCTTGATGCCT
TAACAGAACTTTTTCAGCTTCAGAATCAGGCTCTATAGGTAGATATCTAGGCCCTCTTGG
GTTGAATTTCCTTACTTTTTCCAATGCCTTGATGAATCTCCTGAGCAGGATCACTTTTTT
AAGA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004850A_C01 KMC004850A_c01
         (604 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAN63633.1|AF439853_1 pathogen-induced calmodulin-binding pro...   160  9e-39
gb|AAN63625.1|AF438330_1 pathogen-induced calmodulin-binding pro...   119  3e-26
ref|NP_196022.1| Calmodulin-binding protein; protein id: At5g040...   119  3e-26
gb|AAL48201.1|AF378084_1 PNCBP [Solanum tuberosum]                    119  4e-26
ref|NP_181414.1| Calmodulin-binding protein; protein id: At2g388...   102  4e-21

>gb|AAN63633.1|AF439853_1 pathogen-induced calmodulin-binding protein [Phaseolus vulgaris]
          Length = 178

 Score =  160 bits (406), Expect = 9e-39
 Identities = 81/92 (88%), Positives = 86/92 (93%)
 Frame = -2

Query: 603 LKKVILLRRFIKALEKVRKFNPRGPRYLPIEPDSEAEKVLLRHQDMEARKGQEEWMLDYA 424
           LKKVILLRRFIK+LEKVRKFNPRGPR+LP+E DSEAEKV LRHQDME RKG EEWMLDYA
Sbjct: 87  LKKVILLRRFIKSLEKVRKFNPRGPRHLPLEADSEAEKVNLRHQDMEERKGTEEWMLDYA 146

Query: 423 LRQVVSKLTPARRRKVELLVEAFETVTPTIKS 328
           LRQVVSKLTPAR+RKV LLVEAFETV PTIK+
Sbjct: 147 LRQVVSKLTPARKRKVGLLVEAFETVMPTIKN 178

>gb|AAN63625.1|AF438330_1 pathogen-induced calmodulin-binding protein [Arabidopsis thaliana]
          Length = 1157

 Score =  119 bits (298), Expect = 3e-26
 Identities = 58/89 (65%), Positives = 74/89 (82%)
 Frame = -2

Query: 603  LKKVILLRRFIKALEKVRKFNPRGPRYLPIEPDSEAEKVLLRHQDMEARKGQEEWMLDYA 424
            LK+ ILLRRF+KALE VRKFNPR PR+LP  P+ EAEKV LRHQ+ + +K  +EWM+D A
Sbjct: 1067 LKRAILLRRFVKALENVRKFNPREPRFLPPNPEVEAEKVNLRHQETQNKKNGDEWMVDNA 1126

Query: 423  LRQVVSKLTPARRRKVELLVEAFETVTPT 337
            L+ VVSKLTPAR+ KV+LLV+AFE+++ T
Sbjct: 1127 LQGVVSKLTPARKLKVQLLVQAFESLSAT 1155

 Score =  104 bits (259), Expect = 1e-21
 Identities = 46/87 (52%), Positives = 66/87 (74%)
 Frame = -2

Query: 603  LKKVILLRRFIKALEKVRKFNPRGPRYLPIEPDSEAEKVLLRHQDMEARKGQEEWMLDYA 424
            +KKVILL+RF+  L  + + +P+ PR LP EPD E EK+ LRHQ++  ++  EEWMLDYA
Sbjct: 813  VKKVILLKRFVSDLGSLTRLSPKTPRVLPWEPDPETEKIRLRHQEIGGKRNSEEWMLDYA 872

Query: 423  LRQVVSKLTPARRRKVELLVEAFETVT 343
            LRQ +S L P+++RKV LL +AF+T++
Sbjct: 873  LRQAISTLAPSQKRKVSLLAQAFDTIS 899

 Score = 94.0 bits (232), Expect = 1e-18
 Identities = 51/88 (57%), Positives = 67/88 (75%), Gaps = 2/88 (2%)
 Frame = -2

Query: 603 LKKVILLRRFIKALEKVRKFNPRGPRYLPIEPDSEAEKVLLRHQD-MEARKGQ-EEWMLD 430
           L+KVILL+RF+K+LEKV+  NPR  R LP+E   EAE V LRH+  ME  + + EE MLD
Sbjct: 519 LRKVILLKRFVKSLEKVQVPNPRKMRNLPVESAFEAENVFLRHRSIMEGTRTEGEEMMLD 578

Query: 429 YALRQVVSKLTPARRRKVELLVEAFETV 346
           YALRQ +S+L P +R+KV+LLV+AF+ V
Sbjct: 579 YALRQAISRLAPIQRKKVDLLVQAFDIV 606

 Score = 71.2 bits (173), Expect = 9e-12
 Identities = 38/89 (42%), Positives = 61/89 (67%), Gaps = 1/89 (1%)
 Frame = -2

Query: 603 LKKVILLRRFIKALEKVRKFNPRGPRYLPIEPDSEAEKVL-LRHQDMEARKGQEEWMLDY 427
           LK+VILL+RF+K+L++  +          +    E+E ++ LR + +  RK  EEWMLD+
Sbjct: 181 LKRVILLKRFLKSLDRKERRK--------LSDGKESETIMRLRRELVGERKNAEEWMLDH 232

Query: 426 ALRQVVSKLTPARRRKVELLVEAFETVTP 340
           ALRQV+S L P++++KV+ LV+AFE++ P
Sbjct: 233 ALRQVISTLAPSQKKKVKHLVKAFESLIP 261

>ref|NP_196022.1| Calmodulin-binding protein; protein id: At5g04020.1 [Arabidopsis
            thaliana] gi|11357871|pir||T48429 hypothetical protein
            F8F6.230 - Arabidopsis thaliana
            gi|7406412|emb|CAB85522.1| putative protein [Arabidopsis
            thaliana]
          Length = 1495

 Score =  119 bits (298), Expect = 3e-26
 Identities = 58/89 (65%), Positives = 74/89 (82%)
 Frame = -2

Query: 603  LKKVILLRRFIKALEKVRKFNPRGPRYLPIEPDSEAEKVLLRHQDMEARKGQEEWMLDYA 424
            LK+ ILLRRF+KALE VRKFNPR PR+LP  P+ EAEKV LRHQ+ + +K  +EWM+D A
Sbjct: 1405 LKRAILLRRFVKALENVRKFNPREPRFLPPNPEVEAEKVNLRHQETQNKKNGDEWMVDNA 1464

Query: 423  LRQVVSKLTPARRRKVELLVEAFETVTPT 337
            L+ VVSKLTPAR+ KV+LLV+AFE+++ T
Sbjct: 1465 LQGVVSKLTPARKLKVQLLVQAFESLSAT 1493

 Score =  104 bits (259), Expect = 1e-21
 Identities = 46/87 (52%), Positives = 66/87 (74%)
 Frame = -2

Query: 603  LKKVILLRRFIKALEKVRKFNPRGPRYLPIEPDSEAEKVLLRHQDMEARKGQEEWMLDYA 424
            +KKVILL+RF+  L  + + +P+ PR LP EPD E EK+ LRHQ++  ++  EEWMLDYA
Sbjct: 1151 VKKVILLKRFVSDLGSLTRLSPKTPRVLPWEPDPETEKIRLRHQEIGGKRNSEEWMLDYA 1210

Query: 423  LRQVVSKLTPARRRKVELLVEAFETVT 343
            LRQ +S L P+++RKV LL +AF+T++
Sbjct: 1211 LRQAISTLAPSQKRKVSLLAQAFDTIS 1237

 Score = 94.0 bits (232), Expect = 1e-18
 Identities = 51/88 (57%), Positives = 67/88 (75%), Gaps = 2/88 (2%)
 Frame = -2

Query: 603  LKKVILLRRFIKALEKVRKFNPRGPRYLPIEPDSEAEKVLLRHQD-MEARKGQ-EEWMLD 430
            L+KVILL+RF+K+LEKV+  NPR  R LP+E   EAE V LRH+  ME  + + EE MLD
Sbjct: 857  LRKVILLKRFVKSLEKVQVPNPRKMRNLPVESAFEAENVFLRHRSIMEGTRTEGEEMMLD 916

Query: 429  YALRQVVSKLTPARRRKVELLVEAFETV 346
            YALRQ +S+L P +R+KV+LLV+AF+ V
Sbjct: 917  YALRQAISRLAPIQRKKVDLLVQAFDIV 944

 Score = 71.2 bits (173), Expect = 9e-12
 Identities = 38/89 (42%), Positives = 61/89 (67%), Gaps = 1/89 (1%)
 Frame = -2

Query: 603 LKKVILLRRFIKALEKVRKFNPRGPRYLPIEPDSEAEKVL-LRHQDMEARKGQEEWMLDY 427
           LK+VILL+RF+K+L++  +          +    E+E ++ LR + +  RK  EEWMLD+
Sbjct: 519 LKRVILLKRFLKSLDRKERRK--------LSDGKESETIMRLRRELVGERKNAEEWMLDH 570

Query: 426 ALRQVVSKLTPARRRKVELLVEAFETVTP 340
           ALRQV+S L P++++KV+ LV+AFE++ P
Sbjct: 571 ALRQVISTLAPSQKKKVKHLVKAFESLIP 599

>gb|AAL48201.1|AF378084_1 PNCBP [Solanum tuberosum]
          Length = 1309

 Score =  119 bits (297), Expect = 4e-26
 Identities = 56/88 (63%), Positives = 71/88 (80%)
 Frame = -2

Query: 603  LKKVILLRRFIKALEKVRKFNPRGPRYLPIEPDSEAEKVLLRHQDMEARKGQEEWMLDYA 424
            LKK+ILL+R IKALEK RKFNPR P++LP+ PD E EKV LRHQ  + RK  E+WMLDYA
Sbjct: 1218 LKKLILLKRSIKALEKARKFNPRAPQFLPLTPDQEPEKVDLRHQMTDERKKAEKWMLDYA 1277

Query: 423  LRQVVSKLTPARRRKVELLVEAFETVTP 340
            ++ +V+ LTPAR+++V +LVEAFE V P
Sbjct: 1278 MQHIVTTLTPARKKRVAMLVEAFEAVVP 1305

 Score =  116 bits (291), Expect = 2e-25
 Identities = 57/88 (64%), Positives = 70/88 (78%)
 Frame = -2

Query: 603  LKKVILLRRFIKALEKVRKFNPRGPRYLPIEPDSEAEKVLLRHQDMEARKGQEEWMLDYA 424
            LKK ILL+RF+K LEKVRK NPR P++L + PD EAEKV LR Q  + RK  EEWMLDYA
Sbjct: 753  LKKWILLQRFVKELEKVRKINPRKPQFLQLNPDPEAEKVNLRTQTADERKRGEEWMLDYA 812

Query: 423  LRQVVSKLTPARRRKVELLVEAFETVTP 340
            L+Q +S+L P ++RKVELL++AFETV P
Sbjct: 813  LQQAISQLAPTQQRKVELLIKAFETVVP 840

>ref|NP_181414.1| Calmodulin-binding protein; protein id: At2g38800.1 [Arabidopsis
           thaliana] gi|25408621|pir||E84809 hypothetical protein
           At2g38800 [imported] - Arabidopsis thaliana
           gi|4585899|gb|AAD25561.1| unknown protein [Arabidopsis
           thaliana]
          Length = 612

 Score =  102 bits (254), Expect = 4e-21
 Identities = 48/85 (56%), Positives = 63/85 (73%)
 Frame = -2

Query: 594 VILLRRFIKALEKVRKFNPRGPRYLPIEPDSEAEKVLLRHQDMEARKGQEEWMLDYALRQ 415
           +I  ++ +   E +R+FNPR P YLP   D +AEKV L+HQD++ R+  E+WM DYAL++
Sbjct: 509 IIKCKKPVAETEDLREFNPREPNYLPAVMDEDAEKVDLKHQDIDERRNSEDWMFDYALQR 568

Query: 414 VVSKLTPARRRKVELLVEAFETVTP 340
            VSKL PAR+RKV LLVEAFETV P
Sbjct: 569 AVSKLAPARKRKVALLVEAFETVQP 593

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 474,091,251
Number of Sequences: 1393205
Number of extensions: 9454408
Number of successful extensions: 20398
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 19844
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 20379
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23711793746
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPDL007g08_f AV776882 1 604 2 SPDL044c05_f BP054760 14 567 3 MRL010e05_f BP084213 25 390

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