KMC004824A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC004824A_C01 KMC004824A_c01
agggaagacttattatctattttcaaataaataagtatatacttttataatttttGAAAG
AGAATCCAACAATGGGAATTTCTTATTGATTCAAAGATGATGGAAATGATACAACAATTA
CACATTTATAGTGGAATGCCTAAAGTACCTAATCTAACATTAAACTAACTTTACCAGATA
GCTCATTTTCTAACTAACCATCAACTAACAGTTAACAAACTAACAAAAAAGGGAGATGCA
GAGTTCTGTTCTATCGCCAAAAAGCTTTGTTTGTTATTCAGAGGTTCTGATTGGGAGTAG
AAAATGGAGACAGATCCTCACCATCCTTATCTTTGCTAATAGAAGATTCTCTACCAGTCT
GTTGGAAGCAAATTCCAGTGATTCCAGAGGAATAGATGGCATTATTTCTTAATCACTGGT
AAGGGTTTACTTCGAGCTCACCAGGACAGCATCAGGT


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004824A_C01 KMC004824A_c01
         (457 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||T18400 glutamate-cysteine ligase (EC 6.3.2.2) - malaria par...    31  5.2
ref|NP_704728.1| gamma-glutamylcysteine synthetase [Plasmodium f...    31  5.2
emb|CAA07715.1| gamma-glutamylcysteine synthetase [Plasmodium fa...    31  5.2
emb|CAA07354.1| gamma-glutamylcysteine synthetase [Plasmodium fa...    31  5.2

>pir||T18400 glutamate-cysteine ligase (EC 6.3.2.2) - malaria parasite
           (Plasmodium falciparum) gi|2832348|emb|CAA74990.1|
           gamma-glutamylcysteine synthetase; glutamate--cysteine
           ligase [Plasmodium falciparum]
          Length = 1161

 Score = 31.2 bits (69), Expect = 5.2
 Identities = 16/42 (38%), Positives = 21/42 (49%)
 Frame = +1

Query: 136 MPKVPNLTLN*LYQIAHFLTNHQLTVNKLTKKGDAEFCSIAK 261
           +P  PN+ LN    +A  +T H+ T  K   KG  EF   AK
Sbjct: 188 LPCFPNVLLNNSVLMARRITGHESTKKKFDSKGKVEFIENAK 229

>ref|NP_704728.1| gamma-glutamylcysteine synthetase [Plasmodium falciparum 3D7]
           gi|23505089|emb|CAD51871.1| gamma-glutamylcysteine
           synthetase [Plasmodium falciparum 3D7]
          Length = 1063

 Score = 31.2 bits (69), Expect = 5.2
 Identities = 16/42 (38%), Positives = 21/42 (49%)
 Frame = +1

Query: 136 MPKVPNLTLN*LYQIAHFLTNHQLTVNKLTKKGDAEFCSIAK 261
           +P  PN+ LN    +A  +T H+ T  K   KG  EF   AK
Sbjct: 146 LPCFPNVLLNNSVLMARRITGHESTKKKFDSKGKVEFIENAK 187

>emb|CAA07715.1| gamma-glutamylcysteine synthetase [Plasmodium falciparum]
          Length = 1063

 Score = 31.2 bits (69), Expect = 5.2
 Identities = 16/42 (38%), Positives = 21/42 (49%)
 Frame = +1

Query: 136 MPKVPNLTLN*LYQIAHFLTNHQLTVNKLTKKGDAEFCSIAK 261
           +P  PN+ LN    +A  +T H+ T  K   KG  EF   AK
Sbjct: 146 LPCFPNVLLNNSVLMARRITGHESTKKKFDSKGKVEFIENAK 187

>emb|CAA07354.1| gamma-glutamylcysteine synthetase [Plasmodium falciparum]
          Length = 1118

 Score = 31.2 bits (69), Expect = 5.2
 Identities = 16/42 (38%), Positives = 21/42 (49%)
 Frame = +1

Query: 136 MPKVPNLTLN*LYQIAHFLTNHQLTVNKLTKKGDAEFCSIAK 261
           +P  PN+ LN    +A  +T H+ T  K   KG  EF   AK
Sbjct: 146 LPCFPNVLLNNSVLMARRITGHESTKKKFDSKGKVEFIENAK 187

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 357,875,588
Number of Sequences: 1393205
Number of extensions: 6894825
Number of successful extensions: 10552
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 10382
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 10552
length of database: 448,689,247
effective HSP length: 112
effective length of database: 292,650,287
effective search space used: 11413361193
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MRL007c11_f BP084052 1 459
2 MWM005a01_f AV764717 56 324
3 MRL026c05_f BP085041 57 451
4 MRL019h09_f BP084719 58 394




Lotus japonicus
Kazusa DNA Research Institute