KMC004758A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC004758A_C01 KMC004758A_c01
atgaaAACTAGGGAACCATTTCTTGAATTAGTGGGAAAGAAAGCTCACAAGTACAAAAGC
AACACCAAATATCTACTTCATTGCAACATAAAAGTATACACAAGAGATTGTACTAAGTGG
GAAGTAAAAGGAACCAAGAACATTGAAGGTATAAGGCATTCTTTACTTCGTCAACTATAG
CCGATAGCAAAAACAAGTGCAGGGATCGACAACAGATGACATTTCCAAGCAGGAAGAAGG
AACATACGTTTGAATTTCCAACTCAGTACAATACTCCATGCATTTGCAACTCAACTGGAA
TACCTCAAACAGAAACGATATCTAAAGCACCTCATCAGATCAACAATTATGCCACTTGTA
TAAACTTAAGATGGCAACAAAAACCAAGAAGACGATCAA


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004758A_C01 KMC004758A_c01
         (399 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|EAA16435.1| putative yir4 protein [Plasmodium yoelii yoelii]        33  0.66
gb|AAD03793.1| Tc1-like transposase [Anopheles gambiae]                33  1.1
gb|EAA08715.1| ebiP369 [Anopheles gambiae str. PEST]                   33  1.1
ref|NP_193173.1| disease resistance protein (TIR-NBS-LRR class),...    31  4.2
ref|NP_031473.1| angiogenin [Mus musculus] gi|113874|sp|P21570|A...    31  4.2

>gb|EAA16435.1| putative yir4 protein [Plasmodium yoelii yoelii]
          Length = 299

 Score = 33.5 bits (75), Expect = 0.66
 Identities = 20/61 (32%), Positives = 34/61 (54%), Gaps = 5/61 (8%)
 Frame = +3

Query: 153 KAFFTSSTIADS-KNKCRDRQQMTFPS----RKKEHTFEFPTQYNTPCICNSTGIPQTET 317
           K  FT ST  ++ KN+C+D Q + FPS    +  ++T + P + +   + NS  IP + +
Sbjct: 195 KILFTLSTDYNNLKNECKDAQDVVFPSLPEIKTSQNTTKIPGETSDKNVQNSDVIPSSSS 254

Query: 318 I 320
           I
Sbjct: 255 I 255

>gb|AAD03793.1| Tc1-like transposase [Anopheles gambiae]
          Length = 260

 Score = 32.7 bits (73), Expect = 1.1
 Identities = 22/70 (31%), Positives = 31/70 (43%), Gaps = 7/70 (10%)
 Frame = +1

Query: 40  KAHKYKSNTKYLLHCNIKVYTRDCTKWE-------VKGTKNIEGIRHSLLRQL*PIAKTS 198
           +A+  K+  K L H    V    C  W        +KGT N+EG R  L R++ P A+  
Sbjct: 93  QAYHPKNTIKTLKHGGGHVMVWGCFSWHGPGPLFRIKGTLNLEGYRKILRRKMLPYARQK 152

Query: 199 AGIDNR*HFQ 228
            G +    FQ
Sbjct: 153 FGDEEHYIFQ 162

>gb|EAA08715.1| ebiP369 [Anopheles gambiae str. PEST]
          Length = 260

 Score = 32.7 bits (73), Expect = 1.1
 Identities = 22/70 (31%), Positives = 32/70 (45%), Gaps = 7/70 (10%)
 Frame = +1

Query: 40  KAHKYKSNTKYLLHCNIKVYTRDCTKWE-------VKGTKNIEGIRHSLLRQL*PIAKTS 198
           +A+  K+  K L H    V    C  W        +KGT N+EG R+ L R++ P A+  
Sbjct: 93  QAYHPKNTIKTLSHGGGHVMVWGCFSWHGPGPLFRIKGTLNLEGYRNILRRKMLPHARQQ 152

Query: 199 AGIDNR*HFQ 228
            G +    FQ
Sbjct: 153 FGDEEHYIFQ 162

>ref|NP_193173.1| disease resistance protein (TIR-NBS-LRR class), putative; protein id:
            At4g14370.1 [Arabidopsis thaliana] gi|7488217|pir||F71405
            probable TMV resistance protein - Arabidopsis thaliana
            gi|2244793|emb|CAB10216.1| disease resistance N like
            protein [Arabidopsis thaliana] gi|7268142|emb|CAB78479.1|
            disease resistance N like protein [Arabidopsis thaliana]
          Length = 1996

 Score = 30.8 bits (68), Expect = 4.2
 Identities = 18/55 (32%), Positives = 27/55 (48%)
 Frame = +3

Query: 228  SRKKEHTFEFPTQYNTPCICNSTGIPQTETISKAPHQINNYATCINLRWQQKPRR 392
            +R K+   E P  +++ C   STG P T +I  AP+      T I+L  Q  P +
Sbjct: 1082 NRNKQAKSEIPEIHDSDCF--STGRPSTASIDFAPNNTRRGRTSIDLGIQNSPTK 1134

>ref|NP_031473.1| angiogenin [Mus musculus] gi|113874|sp|P21570|ANGI_MOUSE Angiogenin
           precursor (Ribonuclease 5) (RNase 5)
           gi|109559|pir||A35932 angiogenin precursor - mouse
           gi|726326|gb|AAA91366.1| angiogenin precursor
          Length = 145

 Score = 30.8 bits (68), Expect = 4.2
 Identities = 29/95 (30%), Positives = 41/95 (42%), Gaps = 11/95 (11%)
 Frame = +3

Query: 135 QEH*RYKAFFTSSTIADSKNKCRD---------RQQMTFPSRKKEHTFEFPTQYNTPCIC 287
           Q+  RY  F T     D+K K RD         R+ +T P  K  +TF    + N   IC
Sbjct: 25  QDDSRYTKFLTQHH--DAKPKGRDDRYCERMMKRRSLTSPC-KDVNTFIHGNKSNIKAIC 81

Query: 288 NSTGIPQTETI--SKAPHQINNYATCINLRWQQKP 386
            + G P  E +  SK+P Q+    TC +     +P
Sbjct: 82  GANGSPYRENLRMSKSPFQV---TTCKHTGGSPRP 113

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 331,638,575
Number of Sequences: 1393205
Number of extensions: 6796657
Number of successful extensions: 17521
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 17074
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 17510
length of database: 448,689,247
effective HSP length: 108
effective length of database: 298,223,107
effective search space used: 7157354568
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MWL043d03_f AV769298 1 399
2 MR093b04_f BP083133 6 370
3 MFB052b12_f BP037756 10 221




Lotus japonicus
Kazusa DNA Research Institute