>KMC004735A_C01 KMC004735A_c01
GGTGAAGAACACTGGAAATAATGATAAATTGATAATGATTCCTTCACTAGAGCCAATCGA
AATTTCAATTTCATAGACTAGCTGCAAATAAATTTGCTTTACCCCTTTACCTTATTGAAG
ACTCAATCCATTGCAGCATCATCATCAAACAGAGATATTCATAGGGTACTCTTTGTCCCC
AACATGTAATAAATAAGACCCCTCTTGTATCACCTTTACACCTCCCTCATTTGCAATGCT
AAGATGCTCACAGGGGCTAAGTTCAAACCCAACTTCACCCCTTTCCCCTGGCATCTAGCT
TCACACTTTGAAAACCTACCAACTGTTTTAAAGGGTTTCCATTTCTCTGTTTCCCCTGCC
TCATAAACAGCAACACTGGATGCTTCCCCGCCATGCTTCCATCATTTTTAACTCCCAAAG
TCACCGAGACAGACATGCTTTGACAAGTCTCTTCACCGAGCTCCGAAACCAGCTTGTAAC
GGATGGTTTCGGAGTTTTCATCAACCGTTAAATGAGTGGATGACTGGTTGATGTGAAGGT
TGTTTTGTGTGACGGATACGAACTCGTAGGAGTATTT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004735A_C01 KMC004735A_c01
         (577 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_177929.1| glycosyl hydrolase family 3; protein id: At1g78...    84  1e-26
gb|AAF17692.1|AC009243_19 F28K19.27 [Arabidopsis thaliana]             84  1e-26
gb|AAK38482.1| beta-D-xylosidase [Hordeum vulgare]                     54  3e-15
ref|NP_196618.1| glycosyl hydrolase family 3; protein id: At5g10...    49  3e-11
dbj|BAC41913.1| putative beta-xylosidase [Arabidopsis thaliana]        49  3e-11

>ref|NP_177929.1| glycosyl hydrolase family 3; protein id: At1g78060.1, supported by
           cDNA: gi_18086335 [Arabidopsis thaliana]
           gi|18086336|gb|AAL57631.1| At1g78060/F28K19_32
           [Arabidopsis thaliana]
          Length = 767

 Score = 84.3 bits (207), Expect(2) = 1e-26
 Identities = 44/95 (46%), Positives = 61/95 (63%), Gaps = 2/95 (2%)
 Frame = -1

Query: 574 YSYEFVSVTQNNLHINQSSTHLTVDENSETIRYKLVSELGEETCQSMSVSVTLGVKNDGS 395
           YSY F ++ + NL++NQS        NS+++RY LVSE+G+E C      VT+ V+N G 
Sbjct: 628 YSYRFKTLAETNLYLNQSKAQT----NSDSVRYTLVSEMGKEGCDVAKTKVTVEVENQGE 683

Query: 394 MAGKHPVLLFMR--QGKQRNGNPLKQLVGFQSVKL 296
           MAGKHPVL+F R  +G +      KQLVGF+S+ L
Sbjct: 684 MAGKHPVLMFARHERGGEDGKRAEKQLVGFKSIVL 718

 Score = 57.4 bits (137), Expect(2) = 1e-26
 Identities = 26/47 (55%), Positives = 34/47 (72%)
 Frame = -2

Query: 288 GERGEVGFELSPCEHLSIANEGGVKVIQEGSYLLHVGDKEYPMNISV 148
           GE+ E+ FE+  CEHLS ANE GV V++EG Y L VGD E P+ ++V
Sbjct: 721 GEKAEMEFEIGLCEHLSRANEFGVMVLEEGKYFLTVGDSELPLIVNV 767

>gb|AAF17692.1|AC009243_19 F28K19.27 [Arabidopsis thaliana]
          Length = 696

 Score = 84.3 bits (207), Expect(2) = 1e-26
 Identities = 44/95 (46%), Positives = 61/95 (63%), Gaps = 2/95 (2%)
 Frame = -1

Query: 574 YSYEFVSVTQNNLHINQSSTHLTVDENSETIRYKLVSELGEETCQSMSVSVTLGVKNDGS 395
           YSY F ++ + NL++NQS        NS+++RY LVSE+G+E C      VT+ V+N G 
Sbjct: 557 YSYRFKTLAETNLYLNQSKAQT----NSDSVRYTLVSEMGKEGCDVAKTKVTVEVENQGE 612

Query: 394 MAGKHPVLLFMR--QGKQRNGNPLKQLVGFQSVKL 296
           MAGKHPVL+F R  +G +      KQLVGF+S+ L
Sbjct: 613 MAGKHPVLMFARHERGGEDGKRAEKQLVGFKSIVL 647

 Score = 57.4 bits (137), Expect(2) = 1e-26
 Identities = 26/47 (55%), Positives = 34/47 (72%)
 Frame = -2

Query: 288 GERGEVGFELSPCEHLSIANEGGVKVIQEGSYLLHVGDKEYPMNISV 148
           GE+ E+ FE+  CEHLS ANE GV V++EG Y L VGD E P+ ++V
Sbjct: 650 GEKAEMEFEIGLCEHLSRANEFGVMVLEEGKYFLTVGDSELPLIVNV 696

>gb|AAK38482.1| beta-D-xylosidase [Hordeum vulgare]
          Length = 777

 Score = 54.3 bits (129), Expect(2) = 3e-15
 Identities = 29/66 (43%), Positives = 37/66 (55%)
 Frame = -1

Query: 487 TIRYKLVSELGEETCQSMSVSVTLGVKNDGSMAGKHPVLLFMRQGKQRNGNPLKQLVGFQ 308
           T+ Y  V E+G E C  +     + V+N G M G H VLLF+R     +G P  QL+GFQ
Sbjct: 666 TVSYD-VEEMGAEACDRLRFPAVVRVQNHGPMDGGHLVLLFLRWPNATDGRPASQLIGFQ 724

Query: 307 SVKLDA 290
           SV L A
Sbjct: 725 SVHLRA 730

 Score = 48.5 bits (114), Expect(2) = 3e-15
 Identities = 22/43 (51%), Positives = 30/43 (69%)
 Frame = -2

Query: 285 ERGEVGFELSPCEHLSIANEGGVKVIQEGSYLLHVGDKEYPMN 157
           E   V FE+SPC+HLS A E G KVI +GS+ + VGD E+ ++
Sbjct: 732 EAAHVEFEVSPCKHLSRAAEDGRKVIDQGSHFVRVGDDEFELS 774

>ref|NP_196618.1| glycosyl hydrolase family 3; protein id: At5g10560.1, supported by
           cDNA: gi_15982752 [Arabidopsis thaliana]
           gi|11357198|pir||T49983 beta-xylosidase-like protein -
           Arabidopsis thaliana gi|7671447|emb|CAB89387.1|
           beta-xylosidase-like protein [Arabidopsis thaliana]
           gi|15982753|gb|AAL09717.1| AT5g10560/F12B17_90
           [Arabidopsis thaliana]
          Length = 792

 Score = 49.3 bits (116), Expect(2) = 3e-11
 Identities = 24/75 (32%), Positives = 42/75 (56%)
 Frame = -1

Query: 526 QSSTHLTVDENSETIRYKLVSELGEETCQSMSVSVTLGVKNDGSMAGKHPVLLFMRQGKQ 347
           Q S+H    ++ E +RY  + ++   +C+S+  +V + V N G + G H V+LF +    
Sbjct: 667 QQSSHKKQLQHGEELRYLQLDDVIVNSCESLRFNVRVHVSNTGEIDGSHVVMLFSKMPPV 726

Query: 346 RNGNPLKQLVGFQSV 302
            +G P KQL+G+  V
Sbjct: 727 LSGVPEKQLIGYDRV 741

 Score = 40.0 bits (92), Expect(2) = 3e-11
 Identities = 17/44 (38%), Positives = 30/44 (67%)
 Frame = -2

Query: 285 ERGEVGFELSPCEHLSIANEGGVKVIQEGSYLLHVGDKEYPMNI 154
           E  E  F + PC+ LS+AN+ G +VI  GS++L +GD ++ +++
Sbjct: 747 EMMETVFVIDPCKQLSVANDVGKRVIPLGSHVLFLGDLQHSLSV 790

>dbj|BAC41913.1| putative beta-xylosidase [Arabidopsis thaliana]
          Length = 732

 Score = 49.3 bits (116), Expect(2) = 3e-11
 Identities = 24/75 (32%), Positives = 42/75 (56%)
 Frame = -1

Query: 526 QSSTHLTVDENSETIRYKLVSELGEETCQSMSVSVTLGVKNDGSMAGKHPVLLFMRQGKQ 347
           Q S+H    ++ E +RY  + ++   +C+S+  +V + V N G + G H V+LF +    
Sbjct: 607 QQSSHKKQLQHGEELRYLQLDDVIVNSCESLRFNVRVHVSNTGEIDGSHVVMLFSKMPPV 666

Query: 346 RNGNPLKQLVGFQSV 302
            +G P KQL+G+  V
Sbjct: 667 LSGVPEKQLIGYDRV 681

 Score = 40.0 bits (92), Expect(2) = 3e-11
 Identities = 17/44 (38%), Positives = 30/44 (67%)
 Frame = -2

Query: 285 ERGEVGFELSPCEHLSIANEGGVKVIQEGSYLLHVGDKEYPMNI 154
           E  E  F + PC+ LS+AN+ G +VI  GS++L +GD ++ +++
Sbjct: 687 EMMETVFVIDPCKQLSVANDVGKRVIPLGSHVLFLGDLQHSLSV 730

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 499,520,231
Number of Sequences: 1393205
Number of extensions: 11046695
Number of successful extensions: 28361
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 27526
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 28350
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 21530810025
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF076h11_f BP032350 1 494 2 MR089e11_f BP082852 4 280 3 MRL046f04_f BP085937 21 383 4 MPDL022c06_f AV777605 49 585

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