Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004730A_C01 KMC004730A_c01
(516 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAG33636.1|AF315289_1 beta-carotene hydroxylase [Citrus unshiu] 124 2e-41
gb|AAG10793.1|AF296158_1 beta-carotene hydroxylase [Citrus unshiu] 124 2e-41
emb|CAB55626.1| beta-carotene hydroxylase [Lycopersicon esculentum] 126 2e-41
gb|AAG10430.1| beta hydroxylase [Tagetes erecta] 119 2e-39
ref|NP_194300.1| beta-carotene hydroxylase; protein id: At4g2570... 117 5e-39
>gb|AAG33636.1|AF315289_1 beta-carotene hydroxylase [Citrus unshiu]
Length = 311
Score = 124 bits (312), Expect(2) = 2e-41
Identities = 56/66 (84%), Positives = 61/66 (91%)
Frame = -3
Query: 514 SYGFFHQGLVPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPWAPLPHVPYFRRVAAAHQL 335
S+GFFH+GLVPGLCFGAGLGITVFGMAYMFVHDGLVH+RFP P+ VPYFRRVAAAHQL
Sbjct: 207 SFGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIADVPYFRRVAAAHQL 266
Query: 334 HPSDQF 317
H SD+F
Sbjct: 267 HHSDKF 272
Score = 66.2 bits (160), Expect(2) = 2e-41
Identities = 30/34 (88%), Positives = 34/34 (99%)
Frame = -1
Query: 312 GVPYGLFLGPKELEEVGGLEELEKEINRRIRSYH 211
GVPYGLFLGPKELEEVGGLEELEKEI++RI+SY+
Sbjct: 274 GVPYGLFLGPKELEEVGGLEELEKEISKRIKSYN 307
>gb|AAG10793.1|AF296158_1 beta-carotene hydroxylase [Citrus unshiu]
Length = 311
Score = 124 bits (312), Expect(2) = 2e-41
Identities = 56/66 (84%), Positives = 61/66 (91%)
Frame = -3
Query: 514 SYGFFHQGLVPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPWAPLPHVPYFRRVAAAHQL 335
S+GFFH+GLVPGLCFGAGLGITVFGMAYMFVHDGLVH+RFP P+ VPYFRRVAAAHQL
Sbjct: 207 SFGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIADVPYFRRVAAAHQL 266
Query: 334 HPSDQF 317
H SD+F
Sbjct: 267 HHSDKF 272
Score = 66.2 bits (160), Expect(2) = 2e-41
Identities = 30/34 (88%), Positives = 34/34 (99%)
Frame = -1
Query: 312 GVPYGLFLGPKELEEVGGLEELEKEINRRIRSYH 211
GVPYGLFLGPKELEEVGGLEELEKEI++RI+SY+
Sbjct: 274 GVPYGLFLGPKELEEVGGLEELEKEISKRIKSYN 307
>emb|CAB55626.1| beta-carotene hydroxylase [Lycopersicon esculentum]
Length = 314
Score = 126 bits (316), Expect(2) = 2e-41
Identities = 56/66 (84%), Positives = 62/66 (93%)
Frame = -3
Query: 514 SYGFFHQGLVPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPWAPLPHVPYFRRVAAAHQL 335
SYGFFH+G+VPGLCFGAGLGITVFGMAYMFVHDGLVH+RFP P+ +VPYFRRVAAAHQL
Sbjct: 211 SYGFFHKGIVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRRVAAAHQL 270
Query: 334 HPSDQF 317
H SD+F
Sbjct: 271 HHSDKF 276
Score = 64.3 bits (155), Expect(2) = 2e-41
Identities = 29/31 (93%), Positives = 31/31 (99%)
Frame = -1
Query: 312 GVPYGLFLGPKELEEVGGLEELEKEINRRIR 220
GVPYGLFLGPKELEEVGGLEELEKE+NRRI+
Sbjct: 278 GVPYGLFLGPKELEEVGGLEELEKEVNRRIK 308
>gb|AAG10430.1| beta hydroxylase [Tagetes erecta]
Length = 309
Score = 119 bits (297), Expect(2) = 2e-39
Identities = 52/66 (78%), Positives = 60/66 (90%)
Frame = -3
Query: 514 SYGFFHQGLVPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPWAPLPHVPYFRRVAAAHQL 335
SYGFFH+G++PGLCFGAGLGITVFGMAYMFVHDGLVHRRF P+ +VPY RRVAAAHQL
Sbjct: 206 SYGFFHKGIIPGLCFGAGLGITVFGMAYMFVHDGLVHRRFQVGPIANVPYLRRVAAAHQL 265
Query: 334 HPSDQF 317
H +++F
Sbjct: 266 HHTEKF 271
Score = 64.7 bits (156), Expect(2) = 2e-39
Identities = 29/37 (78%), Positives = 34/37 (91%)
Frame = -1
Query: 315 SGVPYGLFLGPKELEEVGGLEELEKEINRRIRSYHNS 205
+GVPYGLFLGPKELEEVGG EEL+KEI RRI+ Y+N+
Sbjct: 272 NGVPYGLFLGPKELEEVGGTEELDKEIQRRIKLYNNT 308
>ref|NP_194300.1| beta-carotene hydroxylase; protein id: At4g25700.1, supported by
cDNA: gi_13877914, supported by cDNA: gi_21280902,
supported by cDNA: gi_9230269 [Arabidopsis thaliana]
gi|7484824|pir||T09562 beta-carotene hydroxylase homolog
L73G19.80 - Arabidopsis thaliana
gi|4914462|emb|CAB43701.1| beta-carotene hydroxylase
[Arabidopsis thaliana] gi|7269420|emb|CAB81380.1|
beta-carotene hydroxylase [Arabidopsis thaliana]
gi|9230270|gb|AAF85797.1|AF125576_1 beta-carotene
hydroxylase [Arabidopsis thaliana]
gi|9230272|gb|AAF85798.1|AF125577_1 beta-carotene
hydroxylase [Arabidopsis thaliana]
gi|13877915|gb|AAK44035.1|AF370220_1 putative
beta-carotene hydroxylase [Arabidopsis thaliana]
gi|21280903|gb|AAM44971.1| putative beta-carotene
hydroxylase [Arabidopsis thaliana]
Length = 310
Score = 117 bits (293), Expect(2) = 5e-39
Identities = 52/66 (78%), Positives = 60/66 (90%)
Frame = -3
Query: 514 SYGFFHQGLVPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPWAPLPHVPYFRRVAAAHQL 335
SYGFF++GLVPGLCFGAGLGITVFG+AYMFVHDGLVH+RFP P+ VPY R+VAAAHQL
Sbjct: 199 SYGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDGLVHKRFPVGPIADVPYLRKVAAAHQL 258
Query: 334 HPSDQF 317
H +D+F
Sbjct: 259 HHTDKF 264
Score = 65.1 bits (157), Expect(2) = 5e-39
Identities = 30/38 (78%), Positives = 35/38 (91%)
Frame = -1
Query: 315 SGVPYGLFLGPKELEEVGGLEELEKEINRRIRSYHNSS 202
+GVPYGLFLGPKELEEVGG EEL+KEI+RRI+SY +S
Sbjct: 265 NGVPYGLFLGPKELEEVGGNEELDKEISRRIKSYKKAS 302
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 502,356,918
Number of Sequences: 1393205
Number of extensions: 12050985
Number of successful extensions: 35210
Number of sequences better than 10.0: 64
Number of HSP's better than 10.0 without gapping: 32174
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35103
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 16154357632
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)