>KMC004723A_C01 KMC004723A_c01
agtAAAAAACACTAGTAAATATTATAAATAAGTATGCTAATAGTTAAGAATGCTTGCCTT
CCCTCTTTTTAGTTCCCAAATTTAAAAGCACTTCGTGGTCATACAATACAATCATACTCA
TTATCCAGAACAAGCAGATGTAGCAACTGAAGGATCCCTTACAATAGGGAGATGCTTAAA
CAACAAGTCACATTTATTGATTCCAGGAGCACAGCCTCCCAATAGGGAGAATGATATATA
ACAAACCAAATTTATTCTGATTGCAGGAGTCCAGCCTCTCTATAGCTCTCAACCGAATCA
ATGAGTGTTTCCTCCAATGGCCTGGACTTCCAACCCAACCTCTGCAGTTTCTCTGAGGTC
AACCTTCTGTAACTATCCACTTCAGTAAACTTTGTAGGGTACTTGTAATAGGGATATATG
CTCTTTAATTTCTCCACCAAATCCCGTTTCTTGATAGGGTGTGAAGTGCATATGTATCTC
CCTTCTGCCTCAAGCTTCTCATAAGCCAAAAGTACTGCATCAACTAGATCTCCAACATCT
ACTATCCAACTGAGCCTGTTCTCCATCGAGTCAGAACCTTTTAAAAGTTTGAGGAGA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004723A_C01 KMC004723A_c01
         (597 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_180917.1| putative cinnamoyl-CoA reductase; protein id: A...   134  7e-31
ref|NP_180918.1| putative cinnamoyl-CoA reductase; protein id: A...   127  8e-29
gb|AAF16654.1|AC012394_3 putative cinnamoyl-CoA reductase; 14056...   106  2e-22
ref|NP_177773.1| putative cinnamoyl-CoA reductase; protein id: A...   106  2e-22
gb|AAL47182.1| cinnamoyl-CoA reductase [Lolium perenne] gi|17978...    70  3e-11

>ref|NP_180917.1| putative cinnamoyl-CoA reductase; protein id: At2g33590.1,
           supported by cDNA: 11254. [Arabidopsis thaliana]
           gi|25284026|pir||D84747 probable cinnamoyl-CoA reductase
           [imported] - Arabidopsis thaliana
           gi|2459446|gb|AAB80681.1| putative cinnamoyl-CoA
           reductase [Arabidopsis thaliana]
           gi|21536817|gb|AAM61149.1| putative cinnamoyl-CoA
           reductase [Arabidopsis thaliana]
           gi|21539439|gb|AAM53272.1| putative cinnamoyl-CoA
           reductase [Arabidopsis thaliana]
           gi|23197694|gb|AAN15374.1| putative cinnamoyl-CoA
           reductase [Arabidopsis thaliana]
          Length = 321

 Score =  134 bits (338), Expect = 7e-31
 Identities = 66/112 (58%), Positives = 87/112 (76%), Gaps = 1/112 (0%)
 Frame = -2

Query: 596 LLKLLK-GSDSMENRLSWIVDVGDLVDAVLLAYEKLEAEGRYICTSHPIKKRDLVEKLKS 420
           LLKLLK G ++ +N+   +VDV D+  A+LL YEK EAEGRYICTSH +K+  +VEKLKS
Sbjct: 209 LLKLLKEGFETRDNQERHLVDVRDVAQALLLVYEKAEAEGRYICTSHTVKEEIVVEKLKS 268

Query: 419 IYPYYKYPTKFTEVDSYRRLTSEKLQRLGWKSRPLEETLIDSVESYREAGLL 264
            YP+Y YP K+ + +   +++SEKLQ+LGW  RPLEETL+DSVESYR+A L+
Sbjct: 269 FYPHYNYPKKYIDAEDRVKVSSEKLQKLGWTYRPLEETLVDSVESYRKAKLV 320

>ref|NP_180918.1| putative cinnamoyl-CoA reductase; protein id: At2g33600.1,
           supported by cDNA: gi_20260487 [Arabidopsis thaliana]
           gi|25284027|pir||E84747 probable cinnamoyl-CoA reductase
           [imported] - Arabidopsis thaliana
           gi|2459448|gb|AAB80683.1| putative cinnamoyl-CoA
           reductase [Arabidopsis thaliana]
           gi|20260488|gb|AAM13142.1| putative cinnamoyl-CoA
           reductase [Arabidopsis thaliana]
          Length = 321

 Score =  127 bits (320), Expect = 8e-29
 Identities = 63/112 (56%), Positives = 84/112 (74%), Gaps = 1/112 (0%)
 Frame = -2

Query: 596 LLKLLK-GSDSMENRLSWIVDVGDLVDAVLLAYEKLEAEGRYICTSHPIKKRDLVEKLKS 420
           LLKLLK G +S  N+   +VDV D+  A+LL YEK EAEGRYIC  H ++++++ EKLKS
Sbjct: 209 LLKLLKEGYESRNNQERHLVDVRDVAQALLLVYEKAEAEGRYICIGHTVREQEVAEKLKS 268

Query: 419 IYPYYKYPTKFTEVDSYRRLTSEKLQRLGWKSRPLEETLIDSVESYREAGLL 264
           +Y  Y YP ++ E D   +++SEKLQ+LGW  RPLEETL+DSVESYR+A L+
Sbjct: 269 LYLNYNYPKRYIEADGKVKVSSEKLQKLGWTYRPLEETLVDSVESYRKAKLV 320

>gb|AAF16654.1|AC012394_3 putative cinnamoyl-CoA reductase; 14056-15506 [Arabidopsis
           thaliana]
          Length = 320

 Score =  106 bits (265), Expect = 2e-22
 Identities = 58/112 (51%), Positives = 75/112 (66%), Gaps = 3/112 (2%)
 Frame = -2

Query: 596 LLKLLKGS-DSMENRLSWIVDVGDLVDAVLLAYEKLEAEGRYICTSHPIKKRDLVEKLKS 420
           LLK +KG   S+ +   ++VDV D+ DA+LL YE  EA GRYIC SH +    L+EKLK+
Sbjct: 204 LLKFIKGGIKSLLSDELYLVDVRDVADALLLVYENREATGRYICNSHSLYTDSLMEKLKN 263

Query: 419 IYPYYKYPTKFTEV--DSYRRLTSEKLQRLGWKSRPLEETLIDSVESYREAG 270
           +YP   +P  FTEV     R L++EKL+ LGWK RPLEET+ DSV S+  AG
Sbjct: 264 MYPKRNFPESFTEVKEKEVRPLSAEKLKNLGWKFRPLEETIDDSVVSFEAAG 315

>ref|NP_177773.1| putative cinnamoyl-CoA reductase; protein id: At1g76470.1
           [Arabidopsis thaliana] gi|25284028|pir||E96792 probable
           cinnamoyl-CoA reductase, 27707-26257 [imported] -
           Arabidopsis thaliana
           gi|12323980|gb|AAG51951.1|AC015450_12 putative
           cinnamoyl-CoA reductase; 27707-26257 [Arabidopsis
           thaliana]
          Length = 317

 Score =  106 bits (265), Expect = 2e-22
 Identities = 58/112 (51%), Positives = 75/112 (66%), Gaps = 3/112 (2%)
 Frame = -2

Query: 596 LLKLLKGS-DSMENRLSWIVDVGDLVDAVLLAYEKLEAEGRYICTSHPIKKRDLVEKLKS 420
           LLK +KG   S+ +   ++VDV D+ DA+LL YE  EA GRYIC SH +    L+EKLK+
Sbjct: 201 LLKFIKGGIKSLLSDELYLVDVRDVADALLLVYENREATGRYICNSHSLYTDSLMEKLKN 260

Query: 419 IYPYYKYPTKFTEV--DSYRRLTSEKLQRLGWKSRPLEETLIDSVESYREAG 270
           +YP   +P  FTEV     R L++EKL+ LGWK RPLEET+ DSV S+  AG
Sbjct: 261 MYPKRNFPESFTEVKEKEVRPLSAEKLKNLGWKFRPLEETIDDSVVSFEAAG 312

>gb|AAL47182.1| cinnamoyl-CoA reductase [Lolium perenne] gi|17978551|gb|AAL47183.1|
           cinnamoyl-CoA reductase [Lolium perenne]
          Length = 362

 Score = 69.7 bits (169), Expect = 3e-11
 Identities = 40/116 (34%), Positives = 68/116 (58%), Gaps = 5/116 (4%)
 Frame = -2

Query: 596 LLKLLKGSDS-MENRLSWIVDVGDLVDAVLLAYEKLEAEGRYICTSHPIKKRDLVEKLKS 420
           +LK L GS S   N +   VDV D+ DA L  +E   A GR++C    + + D+V  L  
Sbjct: 221 ILKYLDGSASKFANAVQAYVDVRDVADAHLRVFECAAASGRHLCAERVLHREDVVRILAK 280

Query: 419 IYPYYKYPTKFTEVDSYR----RLTSEKLQRLGWKSRPLEETLIDSVESYREAGLL 264
           ++P Y  PT+ ++  + R    +++++KLQ LG + RP+ ++L ++V+S +E G L
Sbjct: 281 LFPEYPVPTRCSDETNPRKQPYKMSNQKLQDLGLEFRPVSQSLYETVKSLQEKGHL 336

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 502,106,788
Number of Sequences: 1393205
Number of extensions: 10782409
Number of successful extensions: 24726
Number of sequences better than 10.0: 164
Number of HSP's better than 10.0 without gapping: 24152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24697
length of database: 448,689,247
effective HSP length: 117
effective length of database: 285,684,262
effective search space used: 23140425222
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF096e02_f BP033303 1 496 2 MPD083e07_f AV775459 4 500 3 MFB020d09_f BP035418 15 599 4 MR086h09_f BP082654 42 407

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