>KMC004702A_C01 KMC004702A_c01
aacaagcaaaacctctactactcttgagaaacaaaattttgagatgatacatgaaacaaa
attatgagattttacatggaACTCGACAATAAAGATTATGAAATTATAATTTTCTTTTGC
TTAAAAAAATTATGAATCTCAGTAAAAAAAATTGAGTAATGATTGATGACTACATCCAAA
TTGGTAATTACATGGTTCACATTTATGTTGTTAAGAGCATTCCCCCTTCCCTCAAAATGA
ATTATGCAATCCACAAGCACAATTGTTTCCACCAACGCTGTTCGCAATGCACGGAGACTT
TCTTTGTCCATCTTGAAACCAAACTTTCCGTTTGTTAACAAGTTCACTAACAAATGAGTC
TAGCATCTCAATGGTGACATGCTGCATCACAACCACATGTGCAACATTTCCTTCACAAGA
CAAATTCCACTTTCGGACAAAGTCATCATCTAGAGGCCTTTCAAATATAACAGTATTACT
AAACTCATTTCGCATTGCACCAATACCATTATCATGTAGTTTATCAAGTAAGTGACTTGC
ATTTGTGATGCATATTTGAGTTT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004702A_C01 KMC004702A_c01
         (563 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_175036.1| histidine decarboxylase, putative; protein id: ...   129  2e-29
dbj|BAA78331.1| serine decarboxylase [Brassica napus]                 124  6e-28
gb|AAG12476.2|AC037197_1 Putative  histidine decarboxylase [Oryz...   108  3e-23
sp|P54772|DCHS_LYCES Histidine decarboxylase (HDC) (TOM92) gi|48...    99  4e-20
gb|ZP_00106716.1| hypothetical protein [Nostoc punctiforme]            62  6e-09

>ref|NP_175036.1| histidine decarboxylase, putative; protein id: At1g43710.1,
           supported by cDNA: gi_13430641, supported by cDNA:
           gi_15011301, supported by cDNA: gi_17907847 [Arabidopsis
           thaliana] gi|25290978|pir||E96500 probable histidine
           decarboxylase [imported] - Arabidopsis thaliana
           gi|7523682|gb|AAF63121.1|AC009526_6 Putative  histidine
           decarboxylase [Arabidopsis thaliana]
           gi|13430642|gb|AAK25943.1|AF360233_1 putative histidine
           decarboxylase [Arabidopsis thaliana]
           gi|14532818|gb|AAK64091.1| putative histidine
           decarboxylase [Arabidopsis thaliana]
           gi|15011302|gb|AAK77493.1|AF389349_1 serine
           decarboxylase [Arabidopsis thaliana]
           gi|17907848|dbj|BAB79456.1| histidine decarboxylase
           [Arabidopsis thaliana] gi|17907853|dbj|BAB79457.1|
           histidine decarboxylase [Arabidopsis thaliana]
          Length = 482

 Score =  129 bits (325), Expect = 2e-29
 Identities = 57/105 (54%), Positives = 79/105 (74%)
 Frame = -3

Query: 558 QICITNASHLLDKLHDNGIGAMRNEFSNTVIFERPLDDDFVRKWNLSCEGNVAHVVVMQH 379
           Q C+ NA +L D+L + GI AM NE S+TV+FERP D++FVR+W L+C+G++AHVVVM  
Sbjct: 378 QKCLRNAHYLKDRLREAGISAMLNELSSTVVFERPKDEEFVRRWQLACQGDIAHVVVMPS 437

Query: 378 VTIEMLDSFVSELVNKRKVWFQDGQRKSPCIANSVGGNNCACGLH 244
           VTIE LD+F+ +LV  R +W++DG  + PC+A+ VG NNC C  H
Sbjct: 438 VTIEKLDNFLKDLVKHRLIWYEDGS-QPPCLASEVGTNNCICPAH 481

>dbj|BAA78331.1| serine decarboxylase [Brassica napus]
          Length = 490

 Score =  124 bits (312), Expect = 6e-28
 Identities = 55/105 (52%), Positives = 76/105 (72%)
 Frame = -3

Query: 558 QICITNASHLLDKLHDNGIGAMRNEFSNTVIFERPLDDDFVRKWNLSCEGNVAHVVVMQH 379
           Q C+ NA +L D+L + GI AM NE S+TV+FERP +++FVR+W L+C+G++AHVVVM  
Sbjct: 386 QKCLRNAHYLKDRLREAGISAMLNELSSTVVFERPKEEEFVRRWQLACQGDIAHVVVMPS 445

Query: 378 VTIEMLDSFVSELVNKRKVWFQDGQRKSPCIANSVGGNNCACGLH 244
           VT+E LD F+ +LV  R VW++DG  + PC+   VG NNC C  H
Sbjct: 446 VTVEKLDHFLKDLVEHRLVWYEDGS-QPPCLVKDVGINNCICPAH 489

>gb|AAG12476.2|AC037197_1 Putative  histidine decarboxylase [Oryza sativa]
           gi|18542940|gb|AAL75763.1|AC069324_20 Putative histidine
           decarboxylase [Oryza sativa]
          Length = 467

 Score =  108 bits (271), Expect = 3e-23
 Identities = 53/104 (50%), Positives = 65/104 (61%)
 Frame = -3

Query: 555 ICITNASHLLDKLHDNGIGAMRNEFSNTVIFERPLDDDFVRKWNLSCEGNVAHVVVMQHV 376
           IC+ NA +L   L   GI A  N  SN V+FERP D+  V +W L+CEGN+AH+VVM +V
Sbjct: 355 ICMGNARYLEVLLKQVGISASCNTLSNIVVFERPKDERIVCRWQLACEGNLAHIVVMPNV 414

Query: 375 TIEMLDSFVSELVNKRKVWFQDGQRKSPCIANSVGGNNCACGLH 244
           T E L  FV EL  KRK W+QD     PC+A  +G  NC C LH
Sbjct: 415 TFEKLTVFVEELAEKRKDWYQDKGFDIPCLAVDIGKENCYCNLH 458

>sp|P54772|DCHS_LYCES Histidine decarboxylase (HDC) (TOM92) gi|481829|pir||S39554
           histidine decarboxylase (EC 4.1.1.22) - tomato
           gi|416534|emb|CAA50719.1| histidine decarboxylase
           [Lycopersicon esculentum]
          Length = 413

 Score = 99.0 bits (245), Expect = 4e-20
 Identities = 49/108 (45%), Positives = 69/108 (63%), Gaps = 4/108 (3%)
 Frame = -3

Query: 552 CITNASHLLDKLHDNGIGAMRNEFSNTVIFERPLDDDFVRKWNLSCEGNVAHVVVMQHVT 373
           CI NA +L D+L + GI  M N+FS TV+FERP D  F+R+WNL C   +AHVV+M  +T
Sbjct: 307 CIENARYLKDRLLEAGISVMLNDFSITVVFERPCDHKFIRRWNLCCLRGMAHVVIMPGIT 366

Query: 372 IEMLDSFVSELVNKRKV-WFQDGQRKSPCIANSVGGNNCACG---LHN 241
            E +DSF  +L+ +R   W+QD +   PC+A+ +   NC C    +HN
Sbjct: 367 RETIDSFFKDLMQERNYKWYQDVKALPPCLADDL-ALNCMCSNKKMHN 413

>gb|ZP_00106716.1| hypothetical protein [Nostoc punctiforme]
          Length = 384

 Score = 61.6 bits (148), Expect = 6e-09
 Identities = 29/72 (40%), Positives = 45/72 (62%)
 Frame = -3

Query: 552 CITNASHLLDKLHDNGIGAMRNEFSNTVIFERPLDDDFVRKWNLSCEGNVAHVVVMQHVT 373
           CI NA +L  +L       M N FSNTV+F++P     ++KW L+   N AH++VMQ++ 
Sbjct: 294 CIHNAQYLFQQLQIREYPCMLNNFSNTVVFQKP-SQRLIKKWQLAVFENWAHMIVMQNIV 352

Query: 372 IEMLDSFVSELV 337
            E +D F++EL+
Sbjct: 353 REKIDIFINELL 364

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 443,420,783
Number of Sequences: 1393205
Number of extensions: 9049773
Number of successful extensions: 23340
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 22424
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23319
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20382500157
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MWM231b10_f AV768248 1 347 2 MR082b12_f BP082290 100 563

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