>KMC004700A_C01 KMC004700A_c01
ccttttgcaaTGCAAAAAAGTTACTTTTTGACACTTAGTTGCATTTGTAGCATTTAATAC
CCACGTAAAGCAGGTGCAACAAAATAGTTATGTGAATTTACGAAGCTCAAAACTCAAAAT
ATTTTGTGGTACCAACACAATCTGTGTAGTCAAAACAGCAATGAAAGGGCCACATCTTCA
TTTTACAGTTGCTCTGGAAATGGATGATGTACATGGTCCACCAGAAGTCATAAATGGAAT
TCCCTTGAATGTAGCCACACCTTTGTCATCAGTGAAAAGATACGGTGTAATCTTGTCCAA
TTGTTTCTTTTTTGGATTAAGTACATCTTCTGGCTTTCCATCAAACCCGGAAAAAGTAAC
TCGCTCCCCAATTTTAGCTCCTTCTGGAGGAAGCAAGGGCTCCACAACTGTGGGACCTTC
ATTGGAAGCACACAAGACCAGTCCTTCAGACATCACATCCCGCAGTTTTCCAGGCTTAAC
ATTAGTAATGAGTACAACACGTCGGTTCGTCAAATCATC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004700A_C01 KMC004700A_c01
         (519 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_565938.1| putative methionyl-tRNA synthetase; protein id:...   183  1e-45
pir||C84832 probable methionyl-tRNA synthetase [imported] - Arab...   183  1e-45
dbj|BAB85305.1| similar to methionyl-tRNA synthetase [Oryza sati...   179  1e-44
ref|NP_193114.1| methionyl-tRNA synthetase - like protein; prote...   108  4e-23
gb|AAH46580.1| Similar to small inducible cytokine subfamily E, ...   105  4e-22

>ref|NP_565938.1| putative methionyl-tRNA synthetase; protein id: At2g40660.1,
           supported by cDNA: gi_14334467, supported by cDNA:
           gi_16323443 [Arabidopsis thaliana]
           gi|14334468|gb|AAK59432.1| putative methionyl-tRNA
           synthetase [Arabidopsis thaliana]
           gi|16323444|gb|AAL15216.1| putative methionyl-tRNA
           synthetase [Arabidopsis thaliana]
          Length = 389

 Score =  183 bits (464), Expect = 1e-45
 Identities = 89/113 (78%), Positives = 101/113 (88%)
 Frame = -1

Query: 519 DDLTNRRVVLITNVKPGKLRDVMSEGLVLCASNEGPTVVEPLLPPEGAKIGERVTFSGFD 340
           +DLTNR V LITNVKPGKLRDVMS+GLVLCAS+E  +VVEPLLPP GAK GERV+FSG +
Sbjct: 277 EDLTNRLVALITNVKPGKLRDVMSQGLVLCASSEDHSVVEPLLPPAGAKPGERVSFSGIE 336

Query: 339 GKPEDVLNPKKKQLDKITPYLFTDDKGVATFKGIPFMTSGGPCTSSISRATVK 181
           GKPEDVLNPKKKQL+KITP L+TD+ GVAT+KGI FMTS GPCTSSI +AT+K
Sbjct: 337 GKPEDVLNPKKKQLEKITPGLYTDENGVATYKGIQFMTSAGPCTSSIPKATIK 389

>pir||C84832 probable methionyl-tRNA synthetase [imported] - Arabidopsis
           thaliana
          Length = 440

 Score =  183 bits (464), Expect = 1e-45
 Identities = 89/113 (78%), Positives = 101/113 (88%)
 Frame = -1

Query: 519 DDLTNRRVVLITNVKPGKLRDVMSEGLVLCASNEGPTVVEPLLPPEGAKIGERVTFSGFD 340
           +DLTNR V LITNVKPGKLRDVMS+GLVLCAS+E  +VVEPLLPP GAK GERV+FSG +
Sbjct: 328 EDLTNRLVALITNVKPGKLRDVMSQGLVLCASSEDHSVVEPLLPPAGAKPGERVSFSGIE 387

Query: 339 GKPEDVLNPKKKQLDKITPYLFTDDKGVATFKGIPFMTSGGPCTSSISRATVK 181
           GKPEDVLNPKKKQL+KITP L+TD+ GVAT+KGI FMTS GPCTSSI +AT+K
Sbjct: 388 GKPEDVLNPKKKQLEKITPGLYTDENGVATYKGIQFMTSAGPCTSSIPKATIK 440

>dbj|BAB85305.1| similar to methionyl-tRNA synthetase [Oryza sativa (japonica
           cultivar-group)] gi|19571060|dbj|BAB86486.1| P0485B12.17
           [Oryza sativa (japonica cultivar-group)]
          Length = 409

 Score =  179 bits (455), Expect = 1e-44
 Identities = 84/113 (74%), Positives = 98/113 (86%)
 Frame = -1

Query: 519 DDLTNRRVVLITNVKPGKLRDVMSEGLVLCASNEGPTVVEPLLPPEGAKIGERVTFSGFD 340
           ++L NR VVLITNVKPGKLRDVMS GLVLCASN+  TVVEPL+PPEGAK GER++F+GFD
Sbjct: 297 EELVNRHVVLITNVKPGKLRDVMSAGLVLCASNQDHTVVEPLIPPEGAKPGERISFAGFD 356

Query: 339 GKPEDVLNPKKKQLDKITPYLFTDDKGVATFKGIPFMTSGGPCTSSISRATVK 181
           GKPEDVLNPKKKQLDKITP L TD  G+AT++G+PF TS GPC SS+  A++K
Sbjct: 357 GKPEDVLNPKKKQLDKITPDLHTDGNGIATYRGVPFTTSAGPCRSSVPNASIK 409

>ref|NP_193114.1| methionyl-tRNA synthetase - like protein; protein id: At4g13780.1,
            supported by cDNA: gi_20259314 [Arabidopsis thaliana]
            gi|12230648|sp|Q9SVN5|SYM_ARATH Probable methionyl-tRNA
            synthetase (Methionine--tRNA ligase) (MetRS)
            gi|7437670|pir||T05247 methionine-tRNA ligase homolog
            F18A5.170 - Arabidopsis thaliana
            gi|4455307|emb|CAB36842.1| methionyl-tRNA synthetase-like
            protein [Arabidopsis thaliana] gi|7268082|emb|CAB78420.1|
            methionyl-tRNA synthetase-like protein [Arabidopsis
            thaliana] gi|17381106|gb|AAL36365.1| putative
            methionyl-tRNA synthetase [Arabidopsis thaliana]
            gi|20259315|gb|AAM14393.1| putative methionyl-tRNA
            synthetase [Arabidopsis thaliana]
          Length = 797

 Score =  108 bits (270), Expect = 4e-23
 Identities = 54/114 (47%), Positives = 79/114 (68%), Gaps = 1/114 (0%)
 Frame = -1

Query: 519  DDLTNRRVVLITNVKPGKLRDVMSEGLVLCASNEGPTVVEPLLPPEGAKIGERVTFSGFD 340
            +++ NR V ++ N+KP K+RD++S+ +VL AS+   + VE + PP+ A IGERVTF GF+
Sbjct: 684  EEMQNRMVCVLCNLKPAKMRDIVSQAMVLAASSSDGSKVELVEPPKTANIGERVTFPGFE 743

Query: 339  GKPEDVLNPKKKQLDKITPYLFTDDKGVATFKGIPFMTSGGPC-TSSISRATVK 181
            G+P+DVLNPKKK  + +   L T +  VA +K +PF TS G C  SSIS  T++
Sbjct: 744  GEPDDVLNPKKKVWETLLVDLNTKENLVACYKDVPFTTSAGVCKVSSISNGTIR 797

>gb|AAH46580.1| Similar to small inducible cytokine subfamily E, member 1 [Xenopus
           laevis]
          Length = 297

 Score =  105 bits (261), Expect = 4e-22
 Identities = 51/103 (49%), Positives = 70/103 (67%)
 Frame = -1

Query: 519 DDLTNRRVVLITNVKPGKLRDVMSEGLVLCASNEGPTVVEPLLPPEGAKIGERVTFSGFD 340
           + + NR  VL+ N+KP K+R ++S+ +V+CAS+  P  VE L PP GA  G+R+TF GF 
Sbjct: 192 EQMQNRMAVLLCNLKPAKMRGILSQAMVMCASS--PEKVEILDPPSGAVPGDRITFQGFP 249

Query: 339 GKPEDVLNPKKKQLDKITPYLFTDDKGVATFKGIPFMTSGGPC 211
           G+P+  LNPKKK  ++I P L T+DK VAT+KG PF   G  C
Sbjct: 250 GEPDKELNPKKKTWEQIQPDLLTNDKCVATYKGAPFEVQGKGC 292

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 464,585,704
Number of Sequences: 1393205
Number of extensions: 10539750
Number of successful extensions: 38839
Number of sequences better than 10.0: 157
Number of HSP's better than 10.0 without gapping: 37064
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 38776
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 16442828304
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MWM003b05_f AV764690 1 519 2 MR081g06_f BP082257 11 373

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