>KMC004654A_C01 KMC004654A_c01
aaaaaataagcctctactggtatatatgcctttcagggaattcatgctttagtgacaggg
gctcacatataacAGCAACAGCTAAATTGAAATCACGCTAAGGATGTTTAGCAAATTCAA
AAACAAAGCCGAAAACAATTCACCAAGCAAGGTCCAGTTCTACTATATAAGTTCAGCAAT
GTTCAAAAAACCACACCAGTAATGAACCTGAATATTCTACATTCATCCATAGTATATGTG
AAATTTACGCAGACTAGAGGTTTTGGTAAATGCCCTGATAAATTCAAGGAGTTGTACAGA
AGCCAGCAACACATTGCAAACGGCCTGATGCCTCATCTTCCATGTCTGGGTGACAAATCA
GCAGATCTTGATCTAGATCGAAGTGGTGATCTTGATTCTCTGCGGGATGACTTCAACTGG
CCTCGATCAGCCTCAGACTTGTATACAGATTTCTCAGTATAGCCATTGCCTTGGTTCTGA
TCAGCACCATTGTCATAAGGTGGAGAGTATGACCTGCGCTTATGATCACCATCTCGCTCC
CTAGAAGGGCTTCTAGGTGATCTAGGGCTTCTAGGTGATCTAGGGTGCTCTGCTTGCCTT
CTAGGGGAAACAGAGTAATCATCACGCCGTCTTGGAG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004654A_C01 KMC004654A_c01
         (637 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

emb|CAC03602.1| SC35-like splicing factor SCL30, 30 kD [Arabidop...    57  2e-07
ref|NP_567021.1| arginine/serine-rich protein SCL30; protein id:...    57  2e-07
pir||T47685 probable RNA binding protein - Arabidopsis thaliana ...    51  1e-05
ref|NP_651272.1| CG13625-PA [Drosophila melanogaster] gi|7301183...    45  8e-04
ref|NP_498134.1| Putative nuclear protein, with a coiled coil do...    42  0.007

>emb|CAC03602.1| SC35-like splicing factor SCL30, 30 kD [Arabidopsis thaliana]
          Length = 262

 Score = 57.4 bits (137), Expect = 2e-07
 Identities = 42/103 (40%), Positives = 53/103 (50%), Gaps = 6/103 (5%)
 Frame = -3

Query: 635 PRRRDDYSVSPRRQAEHPRSPRSPRSPRSPSRERDGDHKRRSYSPPYDNGA------DQN 474
           PRR  D S S  R   +  +PR    P  P  E D ++ RRSYSP Y+  A      D+N
Sbjct: 166 PRRPSD-SRSRYRSRSYSPAPRRRGGP--PRGEEDENYSRRSYSPGYEGAAAAAPDRDRN 222

Query: 473 QGNGYTEKSVYKSEADRGQLKSSRRESRSPLRSRSRSADLSPR 345
             N   EK  Y++E  R   +  R  SRSP  SRSRS ++SPR
Sbjct: 223 GDNEIREKPGYEAEDRR---RGGRAVSRSPSGSRSRSVEVSPR 262

>ref|NP_567021.1| arginine/serine-rich protein SCL30; protein id: At3g55460.1,
           supported by cDNA: 21694., supported by cDNA:
           gi_20466365 [Arabidopsis thaliana]
           gi|20466366|gb|AAM20500.1| putative RNA binding protein
           [Arabidopsis thaliana] gi|21554261|gb|AAM63336.1|
           putative RNA binding protein [Arabidopsis thaliana]
           gi|22136316|gb|AAM91236.1| putative RNA binding protein
           [Arabidopsis thaliana]
          Length = 262

 Score = 57.4 bits (137), Expect = 2e-07
 Identities = 42/103 (40%), Positives = 53/103 (50%), Gaps = 6/103 (5%)
 Frame = -3

Query: 635 PRRRDDYSVSPRRQAEHPRSPRSPRSPRSPSRERDGDHKRRSYSPPYDNGA------DQN 474
           PRR  D S S  R   +  +PR    P  P  E D ++ RRSYSP Y+  A      D+N
Sbjct: 166 PRRPSD-SRSRYRSRSYSPAPRRRGGP--PRGEEDENYSRRSYSPGYEGAAAAAPDRDRN 222

Query: 473 QGNGYTEKSVYKSEADRGQLKSSRRESRSPLRSRSRSADLSPR 345
             N   EK  Y++E  R   +  R  SRSP  SRSRS ++SPR
Sbjct: 223 GDNEIREKPGYEAEDRR---RGGRAVSRSPSGSRSRSVEVSPR 262

>pir||T47685 probable RNA binding protein - Arabidopsis thaliana
           gi|7076789|emb|CAB75904.1| putative RNA binding protein
           [Arabidopsis thaliana]
          Length = 309

 Score = 50.8 bits (120), Expect = 1e-05
 Identities = 39/99 (39%), Positives = 49/99 (49%), Gaps = 6/99 (6%)
 Frame = -3

Query: 635 PRRRDDYSVSPRRQAEHPRSPRSPRSPRSPSRERDGDHKRRSYSPPYDNGA------DQN 474
           PRR  D S S  R   +  +PR    P  P  E D ++ RRSYSP Y+  A      D+N
Sbjct: 196 PRRPSD-SRSRYRSRSYSPAPRRRGGP--PRGEEDENYSRRSYSPGYEGAAAAAPDRDRN 252

Query: 473 QGNGYTEKSVYKSEADRGQLKSSRRESRSPLRSRSRSAD 357
             N   EK  Y++E  R   +  R  SRSP  SRSRS +
Sbjct: 253 GDNEIREKPGYEAEDRR---RGGRAVSRSPSGSRSRSVE 288

>ref|NP_651272.1| CG13625-PA [Drosophila melanogaster] gi|7301183|gb|AAF56315.1|
           CG13625-PA [Drosophila melanogaster]
           gi|15010486|gb|AAK77291.1| GH07383p [Drosophila
           melanogaster]
          Length = 647

 Score = 45.1 bits (105), Expect = 8e-04
 Identities = 36/105 (34%), Positives = 51/105 (48%), Gaps = 8/105 (7%)
 Frame = -3

Query: 632 RRRDDYSVSPRRQAEHPRSPRSPRSPRS-----PSRERDGDH---KRRSYSPPYDNGADQ 477
           RRRDD    PRR+    +SP  PR P+      P R+RD D    ++R  SP   + +DQ
Sbjct: 335 RRRDDKQTPPRRRRNSDQSP--PRRPKDVDQSPPRRKRDFDQSPTRKRDKSPRRRHDSDQ 392

Query: 476 NQGNGYTEKSVYKSEADRGQLKSSRRESRSPLRSRSRSADLSPRH 342
           +    +  +S  +S   R + K  R+ SR    S S+SA   P H
Sbjct: 393 SPARNH--RSRERSPPPRNRFKEERK-SRWAKASPSKSASPPPTH 434

 Score = 34.3 bits (77), Expect = 1.4
 Identities = 25/74 (33%), Positives = 35/74 (46%), Gaps = 7/74 (9%)
 Frame = -3

Query: 635 PRRRDDYSVS-PRRQAEHPRSPRSPRSPRSPSRERDGD------HKRRSYSPPYDNGADQ 477
           PRR  D   S PRR+ +  +SP   R  +SP R  D D      H+ R  SPP  N   +
Sbjct: 355 PRRPKDVDQSPPRRKRDFDQSPTRKRD-KSPRRRHDSDQSPARNHRSRERSPPPRNRFKE 413

Query: 476 NQGNGYTEKSVYKS 435
            + + + + S  KS
Sbjct: 414 ERKSRWAKASPSKS 427

 Score = 34.3 bits (77), Expect = 1.4
 Identities = 31/103 (30%), Positives = 40/103 (38%), Gaps = 4/103 (3%)
 Frame = -3

Query: 632 RRRDDYSVSPRRQAEHPRSPRSPRSPRSPSRERDGDHKRR----SYSPPYDNGADQNQGN 465
           RRRD     PRR     R   S +SP    R++D    RR      SPP    AD     
Sbjct: 245 RRRDSDQSPPRR-----RRSNSDQSPPRRRRDKDSTPPRRRKDSDQSPPRRRKADDQSPV 299

Query: 464 GYTEKSVYKSEADRGQLKSSRRESRSPLRSRSRSADLSPRHGR 336
               K     ++D+   +  R   +SP+R R  S    PR  R
Sbjct: 300 RRERK-----DSDQSPPRKRRDNDQSPVRRRRDSDQSPPRRRR 337

 Score = 31.6 bits (70), Expect = 9.2
 Identities = 32/102 (31%), Positives = 45/102 (43%), Gaps = 7/102 (6%)
 Frame = -3

Query: 632 RRRDDYSVSPRRQAEHPRSP--RSPRSPRSPSRERDGDHKRRSYSPP---YDNGADQNQG 468
           RRRD  S  PRR+ +  +SP  R     +SP R    + K    SPP    DN     + 
Sbjct: 268 RRRDKDSTPPRRRKDSDQSPPRRRKADDQSPVRR---ERKDSDQSPPRKRRDNDQSPVRR 324

Query: 467 NGYTEKSVYKSEADRGQLKSSRRES--RSPLRSRSRSADLSP 348
              +++S  +   D  Q    RR +  +SP R R +  D SP
Sbjct: 325 RRDSDQSPPRRRRDDKQTPPRRRRNSDQSPPR-RPKDVDQSP 365

>ref|NP_498134.1| Putative nuclear protein, with a coiled coil domain, of bilaterial
           origin (52.8 kD) [Caenorhabditis elegans]
           gi|7503937|pir||T16418 hypothetical protein F52C9.7 -
           Caenorhabditis elegans gi|1055054|gb|AAA81056.1|
           Hypothetical protein F52C9.7 [Caenorhabditis elegans]
          Length = 451

 Score = 42.0 bits (97), Expect = 0.007
 Identities = 31/101 (30%), Positives = 44/101 (42%), Gaps = 1/101 (0%)
 Frame = -3

Query: 635 PRRRDDYSVSPRRQAEHPRSPRSPRSPRSPSRERDGDHKRRSYSPPYDNGADQNQGNGYT 456
           P+RR   S  PR+++  PR  R    PRSP R R+ + ++ S  P         +     
Sbjct: 160 PKRRRRPSEKPRKRSRSPRRER----PRSPKRSRE-ESRKLSRKPSSSRSKSPRRSREDP 214

Query: 455 EKSVYKSEADRGQ-LKSSRRESRSPLRSRSRSADLSPRHGR 336
            K   K    R +  +  RR+SRSP RSR     +S    R
Sbjct: 215 RKVARKPSRSRSRSAERLRRKSRSPRRSREEPRKMSRSRSR 255

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 506,076,454
Number of Sequences: 1393205
Number of extensions: 10626481
Number of successful extensions: 41612
Number of sequences better than 10.0: 537
Number of HSP's better than 10.0 without gapping: 34282
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 39641
length of database: 448,689,247
effective HSP length: 118
effective length of database: 284,291,057
effective search space used: 26439068301
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR073f10_f BP081631 1 354 2 SPD064b01_f BP049079 74 637

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