KMC004613A_c01
[Fasta Sequence]   [Nr Search]   [EST assemble image]  

Fasta Sequence
>KMC004613A_C01 KMC004613A_c01
AAATTTAAAATCAAACCTATTTATTGATATCATAAGAAAATCTGTGAAAACTCCTTACAA
AGATTATTCATCTTGCACTTTGGGATGACAATCCTTGGTATTCTTCAGAAAGAAGTAAAT
AATACACCATTTGGGTTTAGAACATAACAAAGTACCTTAGTAGTCAAGGTAAACAGGAGA
GGAAGTTAAAAAGTAGAAAAAGTTCTTGCCGGACATTTCAACCTTCCATATCAAGTTCAT
GGACGTTTCTAACATCACTCTTGTACGCGAAGTCAGCATCAAGATTCTCCATGGTTTTCC
CAAAAGCAAATGACTTTGTACCCATTGTTGTAAACGGCATTAAGCCGAAAGCTCGGGCAG
TTTTAATCTCCCTAGCAACCTTCCTTTGAGCCTTTGCACTAATTTTAGTCATGCTTCTCT
TTTTAAGAATTCCAGCCTCTGTTATGAAGTTTGCCAGGAATCTTACATTCCTGAAATCTG
CCTGACTAAGAACTTCTTTCGTGGTTACTTTGAATTCGGGCCTGATTGGA


Nr search

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004613A_C01 KMC004613A_c01
         (530 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_172201.1| hypothetical protein; protein id: At1g07210.1, ...   136  2e-31
gb|AAM93681.1| putative ribosomal protein [Oryza sativa (japonic...   123  1e-27
pdb|1I94|R Chain R, Crystal Structures Of The Small Ribosomal Su...    53  3e-06
sp|P80382|RS18_THETH 30S ribosomal protein S18 gi|10120581|pdb|1...    53  3e-06
ref|NP_230022.1| ribosomal protein S18 [Vibrio cholerae] gi|1138...    52  4e-06

>ref|NP_172201.1| hypothetical protein; protein id: At1g07210.1, supported by cDNA:
           gi_12083223 [Arabidopsis thaliana]
           gi|25406963|pir||C86207 hypothetical protein [imported]
           - Arabidopsis thaliana
           gi|8954025|gb|AAF82199.1|AC067971_7 Contains similarity
           to a ribosomal protein S18 - Chlamydomonas reinhardtii
           chloroplast (fragment) from Chlamydomonas reinhardtii
           gb|Y16473.  It contains a ribosomal protein S18 domain
           PF|01084.  EST gb|AI997380 comes from this gene.
           [Arabidopsis thaliana]
           gi|12083224|gb|AAG48771.1|AF332408_1 unknown protein
           [Arabidopsis thaliana]
          Length = 261

 Score =  136 bits (342), Expect = 2e-31
 Identities = 68/84 (80%), Positives = 74/84 (87%)
 Frame = -2

Query: 511 KVTTKEVLSQADFRNVRFLANFITEAGILKKRSMTKISAKAQRKVAREIKTARAFGLMPF 332
           +VTT+EVL  ADFRNVRFLANFITEAGI+ KR  T ISAKAQRK+AREIKTARAFGLMPF
Sbjct: 165 EVTTEEVLKNADFRNVRFLANFITEAGIIIKRKQTGISAKAQRKIAREIKTARAFGLMPF 224

Query: 331 TTMGTKSFAFGKTMENLDADFAYK 260
           TTMGTK+F FGKTMEN D DF Y+
Sbjct: 225 TTMGTKAFQFGKTMENRDQDFEYE 248

>gb|AAM93681.1| putative ribosomal protein [Oryza sativa (japonica cultivar-group)]
          Length = 274

 Score =  123 bits (309), Expect = 1e-27
 Identities = 65/102 (63%), Positives = 81/102 (78%), Gaps = 2/102 (1%)
 Frame = -2

Query: 529 PIRPEFKVTTKEVLSQADFRNVRFLANFITEAGILKKRSMTKISAKAQRKVAREIKTARA 350
           P RP+F+ TT+EVL +ADFRNVRFLANF+TEAGI+ KRS T+ISAKAQRKVAREIKTARA
Sbjct: 154 PPRPQFQTTTEEVLKKADFRNVRFLANFLTEAGIIIKRSQTRISAKAQRKVAREIKTARA 213

Query: 349 FGLMPFTTMGTKSFAFGKTME-NL-DADFAYKSDVRNVHELD 230
            GL+PFTTMG + F +G+++E NL + ++ Y    R   E D
Sbjct: 214 LGLLPFTTMGQRPFIYGRSVEVNLSEEEYGYDFVDRRAGEPD 255

>pdb|1I94|R Chain R, Crystal Structures Of The Small Ribosomal Subunit With
           Tetracycline, Edeine And If3 gi|14278568|pdb|1I95|R
           Chain R, Crystal Structure Of The 30s Ribosomal Subunit
           From Thermus Thermophilus In Complex With Edeine
           gi|14278589|pdb|1I96|R Chain R, Crystal Structure Of The
           30s Ribosomal Subunit From Thermus Thermophilus In
           Complex With The Translation Initiation Factor If3
           (C-Terminal Domain) gi|14278611|pdb|1I97|R Chain R,
           Crystal Structure Of The 30s Ribosomal Subunit From
           Thermus Thermophilus In Complex With Tetracycline
          Length = 87

 Score = 52.8 bits (125), Expect = 3e-06
 Identities = 29/68 (42%), Positives = 38/68 (55%), Gaps = 3/68 (4%)
 Frame = -2

Query: 523 RPEFKVTTKEVLSQ---ADFRNVRFLANFITEAGILKKRSMTKISAKAQRKVAREIKTAR 353
           RP  K   K  L +    D+RNV  L  F++E G +  R  T +S K QR +A+ IK AR
Sbjct: 14  RPSRKAKVKATLGEFDLRDYRNVEVLKRFLSETGKILPRRRTGLSGKEQRILAKTIKRAR 73

Query: 352 AFGLMPFT 329
             GL+PFT
Sbjct: 74  ILGLLPFT 81

>sp|P80382|RS18_THETH 30S ribosomal protein S18 gi|10120581|pdb|1FKA|R Chain R, Structure
           Of Functionally Activated Small Ribosomal Subunit At 3.3
           A Resolution gi|10835602|pdb|1FJG|R Chain R, Structure
           Of The Thermus Thermophilus 30s Ribosomal Subunit In
           Complex With The Antibiotics Streptomycin, Spectinomycin
           And Paromomycin gi|11514244|pdb|1G1X|C Chain C,
           Structure Of Ribosomal Proteins S15, S6, S18, And 16s
           Ribosomal Rna gi|11514249|pdb|1G1X|H Chain H, Structure
           Of Ribosomal Proteins S15, S6, S18, And 16s Ribosomal
           Rna gi|13096411|pdb|1HR0|R Chain R, Crystal Structure Of
           Initiation Factor If1 Bound To The 30s Ribosomal Subunit
           gi|13399809|pdb|1HNW|R Chain R, Structure Of The Thermus
           Thermophilus 30s Ribosomal Subunit In Complex With
           Tetracycline gi|13399831|pdb|1HNX|R Chain R, Structure
           Of The Thermus Thermophilus 30s Ribosomal Subunit In
           Complex With Pactamycin gi|13399853|pdb|1HNZ|R Chain R,
           Structure Of The Thermus Thermophilus 30s Ribosomal
           Subunit In Complex With Hygromycin B
           gi|14278027|pdb|1GIX|U Chain U, Crystal Structure Of The
           Ribosome At 5.5 A Resolution. This File, 1gix, Contains
           The 30s Ribosome Subunit, Three Trna, And Mrna
           Molecules. 50s Ribosome Subunit Is In The File 1giy
           gi|14278073|pdb|1IBK|R Chain R, Structure Of The Thermus
           Thermophilus 30s Ribosomal Subunit In Complex With The
           Antibiotic Paromomycin gi|14278094|pdb|1IBL|R Chain R,
           Structure Of The Thermus Thermophilus 30s Ribosomal
           Subunit In Complex With A Messenger Rna Fragment And
           Cognate Transfer Rna Anticodon Stem-Loop Bound At The A
           Site And With The Antibiotic Paromomycin
           gi|14278118|pdb|1IBM|R Chain R, Structure Of The Thermus
           Thermophilus 30s Ribosomal Subunit In Complex With A
           Messenger Rna Fragment And Cognate Transfer Rna
           Anticodon Stem-Loop Bound At The A Site
           gi|15826137|pdb|1JGO|U Chain U, The Path Of Messenger
           Rna Through The Ribosome. This File, 1jgo, Contains The
           30s Ribosome Subunit, Three Trna, And Mrna Molecules.
           50s Ribosome Subunit Is In The File 1giy
           gi|15826162|pdb|1JGP|U Chain U, The Path Of Messenger
           Rna Through The Ribosome. This File, 1jgp, Contains The
           30s Ribosome Subunit, Three Trna, And Mrna Molecules.
           50s Ribosome Subunit Is In The File 1giy
           gi|15826187|pdb|1JGQ|U Chain U, The Path Of Messenger
           Rna Through The Ribosome. This File, 1jgq, Contains The
           30s Ribosome Subunit, Three Trna, And Mrna Molecules.
           50s Ribosome Subunit Is In The File 1giy
           gi|20663903|pdb|1J5E|R Chain R, Structure Of The Thermus
           Thermophilus 30s Ribosomal Subunit
           gi|27065893|pdb|1N32|R Chain R, Structure Of The Thermus
           Thermophilus 30s Ribosomal Subunit Bound To Codon And
           Near-Cognate Transfer Rna Anticodon Stem-Loop Mismatched
           At The First Codon Position At The A Site With
           Paromomycin gi|27065920|pdb|1N33|R Chain R, Structure Of
           The Thermus Thermophilus 30s Ribosomal Subunit Bound To
           Codon And Near-Cognate Transfer Rna Anticodon Stem-Loop
           Mismatched At The Second Codon Position At The A Site
           With Paromomycin gi|27065947|pdb|1N34|R Chain R,
           Structure Of The Thermus Thermophilus 30s Ribosomal
           Subunit In The Presence Of Codon And
           Crystallographically Disordered Near-Cognate Transfer
           Rna Anticodon Stem-Loop Mismatched At The First Codon
           Position gi|27065973|pdb|1N36|R Chain R, Structure Of
           The Thermus Thermophilus 30s Ribosomal Subunit In The
           Presence Of Crystallographically Disordered Codon And
           Near-Cognate Transfer Rna Anticodon Stem-Loop Mismatched
           At The Second Codon Position gi|28373716|pdb|1ML5|U
           Chain U, Structure Of The E. Coli Ribosomal Termination
           Complex With Release Factor 2
           gi|6739549|gb|AAF27297.1|AF146075_3 ribosomal protein
           S18 [Thermus thermophilus]
          Length = 88

 Score = 52.8 bits (125), Expect = 3e-06
 Identities = 29/68 (42%), Positives = 38/68 (55%), Gaps = 3/68 (4%)
 Frame = -2

Query: 523 RPEFKVTTKEVLSQ---ADFRNVRFLANFITEAGILKKRSMTKISAKAQRKVAREIKTAR 353
           RP  K   K  L +    D+RNV  L  F++E G +  R  T +S K QR +A+ IK AR
Sbjct: 15  RPSRKAKVKATLGEFDLRDYRNVEVLKRFLSETGKILPRRRTGLSGKEQRILAKTIKRAR 74

Query: 352 AFGLMPFT 329
             GL+PFT
Sbjct: 75  ILGLLPFT 82

>ref|NP_230022.1| ribosomal protein S18 [Vibrio cholerae]
           gi|11387102|sp|Q9KUZ0|RS18_VIBCH 30S ribosomal protein
           S18 gi|11356096|pir||B82333 ribosomal protein S18 VC0368
           [imported] - Vibrio cholerae  (strain N16961 serogroup
           O1) gi|9654785|gb|AAF93541.1| ribosomal protein S18
           [Vibrio cholerae]
          Length = 75

 Score = 52.0 bits (123), Expect = 4e-06
 Identities = 28/65 (43%), Positives = 41/65 (63%)
 Frame = -2

Query: 523 RPEFKVTTKEVLSQADFRNVRFLANFITEAGILKKRSMTKISAKAQRKVAREIKTARAFG 344
           R +F   T E + + D+++V  L N+ITEAG +    +T  SAK QR++AR IK AR   
Sbjct: 7   RRKFCRFTAEGVQEIDYKDVATLKNYITEAGKIVPSRITGTSAKYQRQLARAIKRARYLA 66

Query: 343 LMPFT 329
           L+P+T
Sbjct: 67  LLPYT 71

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 411,617,545
Number of Sequences: 1393205
Number of extensions: 8073539
Number of successful extensions: 17959
Number of sequences better than 10.0: 107
Number of HSP's better than 10.0 without gapping: 17664
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 17953
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 17596710992
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)


EST assemble image


clone accession position
1 MPDL045d10_f AV778780 1 474
2 SPD097d02_f BP051743 1 531
3 MR067c10_f BP081143 1 398
4 MFB015g08_f BP035050 44 489




Lotus japonicus
Kazusa DNA Research Institute