>KMC004574A_C01 KMC004574A_c01
tttgAAGACAAACCATTTATTTATTTATTCATGGCAAACTTCTTAATTCATTTTAAAGCA
AATGCTCTTCTCCACTATGGAGAACAAAGACAAACCACAAATTTATTGCAATCTAAAACC
AATATTAAGAAATGGTTTCAAATTATCCCAAGGACAGAGGCTCAAATTATTTCTGATTAG
GCACCTTGATATAGCCTACATGGGTGGAATTCGCCACTCTAGTAAGGGTTTGTAGATCAT
ACGCTGCCCAACACCTTGAGTTATCCTTGTTGTAGAAATACCCCACACACTTGCAGTCTT
TGGTGCATTTGTTTCCACAATTAGTTTCACTTACTCTATCCCCAGTAGTGTATTTACTCA
TGTAATGCTCAACTCCTTCAAGTTTGTAGTAGTGGAATTCACTTGCCTTGCATGATGTTA
CAGCCTTAGCACTGCAATTGTTACTCCACCCAAATAGTCCATTCTCCAATGGGCAAGCAA
CACATTGATTTCCCTCACAAAGCCC

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004574A_C01 KMC004574A_c01
         (505 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAO15899.1| secreted glycoprotein [Linum usitatissimum]            146  1e-34
gb|AAN31898.1| putative glycoprotein (EP1) [Arabidopsis thaliana]     144  7e-34
ref|NP_178006.1| glycoprotein(EP1), putative; protein id: At1g78...   144  7e-34
pir||E86304 F6I1.9 protein - Arabidopsis thaliana gi|9802773|gb|...   142  2e-33
dbj|BAC42448.1| unknown protein [Arabidopsis thaliana]                142  2e-33

>gb|AAO15899.1| secreted glycoprotein [Linum usitatissimum]
          Length = 430

 Score =  146 bits (368), Expect = 1e-34
 Identities = 63/112 (56%), Positives = 84/112 (74%), Gaps = 3/112 (2%)
 Frame = -1

Query: 505 GLCEGNQCVACPLENGLFGWSNNCS--AKAVTSCKASEFHYYKLEGVEHYMSKYTTGDR- 335
           GLCE + CVACP  NGL GWS  C   A +V+SC   +F Y++L+GV+H+M+KYT GD  
Sbjct: 317 GLCEDSMCVACPTPNGLTGWSKECKPPAVSVSSCSGRKFKYFELKGVDHFMAKYTRGDSP 376

Query: 334 VSETNCGNKCTKDCKCVGYFYNKDNSRCWAAYDLQTLTRVANSTHVGYIKVP 179
           V+++ C  KC+KDCKC+GYFY  D+SRCW A +L+T++RVAN TH+ YIK P
Sbjct: 377 VAKSACEEKCSKDCKCLGYFYQSDSSRCWIANELKTMSRVANQTHLAYIKTP 428

>gb|AAN31898.1| putative glycoprotein (EP1) [Arabidopsis thaliana]
          Length = 441

 Score =  144 bits (362), Expect = 7e-34
 Identities = 55/110 (50%), Positives = 78/110 (70%)
 Frame = -1

Query: 505 GLCEGNQCVACPLENGLFGWSNNCSAKAVTSCKASEFHYYKLEGVEHYMSKYTTGDRVSE 326
           GLC+  QC ACP + GL GW   C + ++ SC    FHY+K+EG + +M+KY  G   +E
Sbjct: 325 GLCKKGQCNACPSDKGLLGWDETCKSPSLASCDPKTFHYFKIEGADSFMTKYNGGSSTTE 384

Query: 325 TNCGNKCTKDCKCVGYFYNKDNSRCWAAYDLQTLTRVANSTHVGYIKVPN 176
           + CG+KCT+DCKC+G+FYN+ +SRCW  Y+L+TLTR  +S+ V Y+K PN
Sbjct: 385 SACGDKCTRDCKCLGFFYNRKSSRCWLGYELKTLTRTGDSSLVAYVKTPN 434

>ref|NP_178006.1| glycoprotein(EP1), putative; protein id: At1g78850.1, supported by
           cDNA: gi_15450841 [Arabidopsis thaliana]
           gi|25406575|pir||H96817 hypothetical protein F9K20.10
           [imported] - Arabidopsis thaliana
           gi|3834309|gb|AAC83025.1| Strong similarity to
           glycoprotein EP1 gb|L16983 Daucus carota and a member of
           S locus glycoprotein family PF|00954.  ESTs gb|F13813,
           gb|T21052, gb|R30218 and gb|W43262 come from this gene.
           [Arabidopsis thaliana] gi|15450842|gb|AAK96692.1| Strong
           similarity to glycoprotein EP1 [Arabidopsis thaliana]
          Length = 441

 Score =  144 bits (362), Expect = 7e-34
 Identities = 55/110 (50%), Positives = 78/110 (70%)
 Frame = -1

Query: 505 GLCEGNQCVACPLENGLFGWSNNCSAKAVTSCKASEFHYYKLEGVEHYMSKYTTGDRVSE 326
           GLC+  QC ACP + GL GW   C + ++ SC    FHY+K+EG + +M+KY  G   +E
Sbjct: 325 GLCKKGQCNACPSDKGLLGWDETCKSPSLASCDPKTFHYFKIEGADSFMTKYNGGSSTTE 384

Query: 325 TNCGNKCTKDCKCVGYFYNKDNSRCWAAYDLQTLTRVANSTHVGYIKVPN 176
           + CG+KCT+DCKC+G+FYN+ +SRCW  Y+L+TLTR  +S+ V Y+K PN
Sbjct: 385 SACGDKCTRDCKCLGFFYNRKSSRCWLGYELKTLTRTGDSSLVAYVKAPN 434

>pir||E86304 F6I1.9 protein - Arabidopsis thaliana
           gi|9802773|gb|AAF99842.1|AC051629_9 Hypothetical protein
           [Arabidopsis thaliana]
          Length = 397

 Score =  142 bits (358), Expect = 2e-33
 Identities = 59/110 (53%), Positives = 76/110 (68%)
 Frame = -1

Query: 505 GLCEGNQCVACPLENGLFGWSNNCSAKAVTSCKASEFHYYKLEGVEHYMSKYTTGDRVSE 326
           G+CE NQCVACPL  GL GWS  C  K V SC    FHYY+L GVEH+M+KY  G  + E
Sbjct: 287 GICEDNQCVACPLGVGLMGWSKACKPKKVKSCDPKSFHYYRLGGVEHFMTKYNVGLALGE 346

Query: 325 TNCGNKCTKDCKCVGYFYNKDNSRCWAAYDLQTLTRVANSTHVGYIKVPN 176
           + C   C+ DCKC+GYF++K + +CW +Y+L TL +V++S  V YIK PN
Sbjct: 347 SKCRGLCSGDCKCLGYFFDKSSFKCWISYELGTLVKVSDSRKVAYIKTPN 396

>dbj|BAC42448.1| unknown protein [Arabidopsis thaliana]
          Length = 423

 Score =  142 bits (358), Expect = 2e-33
 Identities = 59/110 (53%), Positives = 76/110 (68%)
 Frame = -1

Query: 505 GLCEGNQCVACPLENGLFGWSNNCSAKAVTSCKASEFHYYKLEGVEHYMSKYTTGDRVSE 326
           G+CE NQCVACPL  GL GWS  C  K V SC    FHYY+L GVEH+M+KY  G  + E
Sbjct: 313 GICEDNQCVACPLGVGLMGWSKACKPKKVKSCDPKSFHYYRLGGVEHFMTKYNVGLALGE 372

Query: 325 TNCGNKCTKDCKCVGYFYNKDNSRCWAAYDLQTLTRVANSTHVGYIKVPN 176
           + C   C+ DCKC+GYF++K + +CW +Y+L TL +V++S  V YIK PN
Sbjct: 373 SKCRGLCSGDCKCLGYFFDKSSFKCWISYELGTLVKVSDSRKVAYIKTPN 422

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 437,520,296
Number of Sequences: 1393205
Number of extensions: 8920089
Number of successful extensions: 21535
Number of sequences better than 10.0: 67
Number of HSP's better than 10.0 without gapping: 20787
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21503
length of database: 448,689,247
effective HSP length: 114
effective length of database: 289,863,877
effective search space used: 15362785481
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFB029h02_f BP036148 1 494 2 MPDL050c04_f AV779024 5 507 3 MR061e03_f BP080681 13 229

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