>KMC004523A_C01 KMC004523A_c01
GTATTCAAATTGAATTTCTTTGAAGTCGAACATATTCAAAATGAATAATTATTCATGATA
GCATCAAACTAATTAAGAAGACAACTATGAACTAAAACTAAAACGACTGAACAAGGTGCT
TGATATCGATTGGATAAGTGAAGGCTGGATTAGAAGCATTATATGAAGCAAATGCAGTGA
ATATGTTGGCAGCTTTTGTAGGATGGAATTCATCCCAGAAAATATACTCATTCCTATTCT
GGCATGGAATTTGATCACGAATACAAAGTCCATGTGCCCCTGTTTTGCAGCAGCTTGCAT
TCAAAACTTTGAAACCAAGTGAATTGCCAAGGGATCCAGCTGTACTGTTTATGAAGATGA
ATTTAGAATCAGCAGAGAACTTGTGATTGAATTTTGGCACAGAAGATTTAAGCTTGTTGT
TAAATATGATTGCAGCAGCATTCTCCTCTTCAACACAAGCTCCATTTGTCCCACGAGTAG
AAATG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004523A_C01 KMC004523A_c01
         (485 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_174260.1| lipase/hydrolase, putative; protein id: At1g296...   104  6e-22
gb|AAM64368.1| lipase/hydrolase, putative [Arabidopsis thaliana]      104  6e-22
gb|AAM65973.1| lipase/hydrolase, putative [Arabidopsis thaliana]       96  2e-19
ref|NP_174259.1| lipase/hydrolase, putative; protein id: At1g296...    96  2e-19
gb|AAM63021.1| GDSL-motif lipase/hydrolase-like protein [Arabido...    87  1e-16

>ref|NP_174260.1| lipase/hydrolase, putative; protein id: At1g29670.1, supported by
           cDNA: 23556., supported by cDNA: gi_18086454
           [Arabidopsis thaliana] gi|25336248|pir||A86420 probable
           lipase/hydrolase, 118270-120144 [imported] - Arabidopsis
           thaliana gi|12323546|gb|AAG51758.1|AC068667_37
           lipase/hydrolase, putative; 118270-120144 [Arabidopsis
           thaliana] gi|18086455|gb|AAL57681.1| At1g29670/F15D2_22
           [Arabidopsis thaliana]
          Length = 363

 Score =  104 bits (259), Expect = 6e-22
 Identities = 56/125 (44%), Positives = 76/125 (60%), Gaps = 8/125 (6%)
 Frame = -2

Query: 457 CVEEENAAAIIFNNKLKSSVPKFNHKFSADSKFIFINSTA---GSLGNS--LGFKVLNAS 293
           CV+  N+A  IFNNKL+S V + N+    D+KFI+IN+       + N    GF+V NA 
Sbjct: 239 CVDRINSANQIFNNKLRSLVDQLNNNHP-DAKFIYINAYGIFQDMITNPARFGFRVTNAG 297

Query: 292 CCKTGAHG---LCIRDQIPCQNRNEYIFWDEFHPTKAANIFTAFASYNASNPAFTYPIDI 122
           CC  G +     C+  Q PC++RN Y+FWD FHPT+AAN+  A  SYNA + +  YP+DI
Sbjct: 298 CCGIGRNAGQITCLPGQRPCRDRNAYVFWDAFHPTEAANVIIARRSYNAQSASDAYPMDI 357

Query: 121 KHLVQ 107
             L Q
Sbjct: 358 SRLAQ 362

>gb|AAM64368.1| lipase/hydrolase, putative [Arabidopsis thaliana]
          Length = 363

 Score =  104 bits (259), Expect = 6e-22
 Identities = 56/125 (44%), Positives = 76/125 (60%), Gaps = 8/125 (6%)
 Frame = -2

Query: 457 CVEEENAAAIIFNNKLKSSVPKFNHKFSADSKFIFINSTA---GSLGNS--LGFKVLNAS 293
           CV+  N+A  IFNNKL+S V + N+    D+KFI+IN+       + N    GF+V NA 
Sbjct: 239 CVDRINSANQIFNNKLRSLVDQLNNNHP-DAKFIYINAYGIFQDMITNPARFGFRVTNAG 297

Query: 292 CCKTGAHG---LCIRDQIPCQNRNEYIFWDEFHPTKAANIFTAFASYNASNPAFTYPIDI 122
           CC  G +     C+  Q PC++RN Y+FWD FHPT+AAN+  A  SYNA + +  YP+DI
Sbjct: 298 CCGIGRNAGQITCLPGQRPCRDRNAYVFWDAFHPTEAANVIIARRSYNAQSASDAYPMDI 357

Query: 121 KHLVQ 107
             L Q
Sbjct: 358 SRLAQ 362

>gb|AAM65973.1| lipase/hydrolase, putative [Arabidopsis thaliana]
          Length = 364

 Score = 95.9 bits (237), Expect = 2e-19
 Identities = 53/125 (42%), Positives = 71/125 (56%), Gaps = 8/125 (6%)
 Frame = -2

Query: 457 CVEEENAAAIIFNNKLKSSVPKFNHKFSADSKFIFINSTAG-----SLGNSLGFKVLNAS 293
           CVE  N+A  IFNN+L S V + N+  S D+ F +IN+        +  ++ GF   N +
Sbjct: 240 CVERINSANRIFNNRLISMVQQLNNAHS-DASFTYINAYGAFQDIITNPSAYGFTNTNTA 298

Query: 292 CCKTGAHG---LCIRDQIPCQNRNEYIFWDEFHPTKAANIFTAFASYNASNPAFTYPIDI 122
           CC  G +G    C+  + PC NR+EY+FWD FHP+ AAN   A  SYNA   +  YPIDI
Sbjct: 299 CCGIGRNGGQLTCLPGEPPCLNRDEYVFWDAFHPSAAANTAIAKRSYNAQRSSDVYPIDI 358

Query: 121 KHLVQ 107
             L Q
Sbjct: 359 SQLAQ 363

>ref|NP_174259.1| lipase/hydrolase, putative; protein id: At1g29660.1, supported by
           cDNA: 6680., supported by cDNA: gi_15215767 [Arabidopsis
           thaliana] gi|25336246|pir||H86419 probable
           lipase/hydrolase, 114382-116051 [imported] - Arabidopsis
           thaliana gi|12323544|gb|AAG51756.1|AC068667_35
           lipase/hydrolase, putative; 114382-116051 [Arabidopsis
           thaliana] gi|15215768|gb|AAK91429.1| At1g29660/F15D2_21
           [Arabidopsis thaliana] gi|22137090|gb|AAM91390.1|
           At1g29660/F15D2_21 [Arabidopsis thaliana]
          Length = 364

 Score = 95.9 bits (237), Expect = 2e-19
 Identities = 53/125 (42%), Positives = 71/125 (56%), Gaps = 8/125 (6%)
 Frame = -2

Query: 457 CVEEENAAAIIFNNKLKSSVPKFNHKFSADSKFIFINSTAG-----SLGNSLGFKVLNAS 293
           CVE  N+A  IFNN+L S V + N+  S D+ F +IN+        +  ++ GF   N +
Sbjct: 240 CVERINSANRIFNNRLISMVQQLNNAHS-DASFTYINAYGAFQDIIANPSAYGFTNTNTA 298

Query: 292 CCKTGAHG---LCIRDQIPCQNRNEYIFWDEFHPTKAANIFTAFASYNASNPAFTYPIDI 122
           CC  G +G    C+  + PC NR+EY+FWD FHP+ AAN   A  SYNA   +  YPIDI
Sbjct: 299 CCGIGRNGGQLTCLPGEPPCLNRDEYVFWDAFHPSAAANTAIAKRSYNAQRSSDVYPIDI 358

Query: 121 KHLVQ 107
             L Q
Sbjct: 359 SQLAQ 363

>gb|AAM63021.1| GDSL-motif lipase/hydrolase-like protein [Arabidopsis thaliana]
          Length = 362

 Score = 87.0 bits (214), Expect = 1e-16
 Identities = 51/123 (41%), Positives = 67/123 (54%), Gaps = 8/123 (6%)
 Frame = -2

Query: 457 CVEEENAAAIIFNNKLKSSVPKFNHKFSADSKFIFINSTA---GSLGNSL--GFKVLNAS 293
           C E  N+A  IFN+KL S V  FN   + D+KF +IN+       + N    GF+V NA 
Sbjct: 238 CDERINSANRIFNSKLISIVDAFNQN-TPDAKFTYINAYGIFQDIITNPARYGFRVTNAG 296

Query: 292 CCKTGAHG---LCIRDQIPCQNRNEYIFWDEFHPTKAANIFTAFASYNASNPAFTYPIDI 122
           CC  G +     C+  Q PC NRNEY+FWD FHP +AANI     S+     +  +P DI
Sbjct: 297 CCGVGRNNGQITCLPGQAPCLNRNEYVFWDAFHPGEAANIVIGRRSFKREAASNAHPYDI 356

Query: 121 KHL 113
           + L
Sbjct: 357 QQL 359

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 370,986,139
Number of Sequences: 1393205
Number of extensions: 7278682
Number of successful extensions: 25264
Number of sequences better than 10.0: 181
Number of HSP's better than 10.0 without gapping: 24626
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25148
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 13980339936
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR053a02_f BP080048 1 485 2 MWM150b05_f AV767069 1 415

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