Nr search
BLASTX 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KMC004512A_C01 KMC004512A_c01
(388 letters)
Database: nr
1,393,205 sequences; 448,689,247 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAL66194.1|AF386512_1 cytochrome P450 [Pyrus communis] 96 8e-20
ref|NP_680110.1| Expressed protein; protein id: At3g48310.1, sup... 52 7e-10
gb|AAO17011.1| Hypothetical protein [Oryza sativa (japonica cult... 55 1e-09
ref|NP_680111.1| similar to cytochrome P450-like protein; protei... 55 2e-09
ref|NP_197878.1| cytochrome P450-like protein; protein id: At5g2... 51 6e-09
>gb|AAL66194.1|AF386512_1 cytochrome P450 [Pyrus communis]
Length = 506
Score = 96.3 bits (238), Expect = 8e-20
Identities = 49/102 (48%), Positives = 71/102 (69%)
Frame = +3
Query: 21 MQLLTISKVDSFESLRKREMLLTVTSLEKAAKAGQVVDLSEVMHNLLEDIVYKMVLGCSK 200
+QLL SK++SF LR+ E+ L V SL+ AA+AG+VVD SE + L+E I Y+MVLG
Sbjct: 135 LQLLCPSKIESFAPLRREEVGLLVQSLKVAAEAGEVVDFSEKVGELVEGITYRMVLGRKN 194
Query: 201 VSGFDLKGLIQRFMYLLGAFNLADHVPCLGALDXSGIKTKLQ 326
FDLKG+I+ ++L GAFN++D+VP L LD G+ +++
Sbjct: 195 DDMFDLKGIIEEALFLTGAFNISDYVPFLSPLDLQGLTKRMK 236
Score = 38.5 bits (88), Expect = 0.021
Identities = 24/55 (43%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
Frame = +1
Query: 223 GLFN--DLCTFLEPLI*QIMYLVLELWTLQGLKRSFKETSKALDQVLEKIIQEHE 381
G FN D FL PL LQGL + K SK +DQ+ EKIIQ+HE
Sbjct: 212 GAFNISDYVPFLSPL------------DLQGLTKRMKRVSKTVDQLFEKIIQDHE 254
>ref|NP_680110.1| Expressed protein; protein id: At3g48310.1, supported by cDNA:
gi_20465426 [Arabidopsis thaliana]
gi|13878403|sp|Q9STL1|C71M_ARATH Cytochrome P450 71A22
gi|7430647|pir||T06711 probable cytochrome P450
T29H11.170 - Arabidopsis thaliana
gi|4678357|emb|CAB41167.1| cytochrome P450-like protein
[Arabidopsis thaliana] gi|20465427|gb|AAM20137.1|
unknown protein [Arabidopsis thaliana]
gi|22136888|gb|AAM91788.1| unknown protein [Arabidopsis
thaliana]
Length = 490
Score = 52.0 bits (123), Expect(2) = 7e-10
Identities = 33/103 (32%), Positives = 59/103 (57%), Gaps = 1/103 (0%)
Frame = +3
Query: 21 MQLLTISKVDSFESLRKREMLLTVTSLEKAAKAGQVVDLSEVMHNLLEDIVYKMVLGCSK 200
+ LLT V SF ++R+ E+ L + ++K++ V+LSE++ +L D++ ++ LG
Sbjct: 133 LHLLTNKMVRSFRNVRQEEISLMMEKIQKSSSLQ--VNLSELLGSLTNDVISRVALGRKY 190
Query: 201 VSGFDLKGLIQRFMYLLGAFNLADHVPCLGALD-XSGIKTKLQ 326
D K L++R LLG F + +VP L +D SG+ +L+
Sbjct: 191 SDETDFKELMKRLTKLLGEFCVGTYVPWLAWIDWISGLDGQLK 233
Score = 31.2 bits (69), Expect(2) = 7e-10
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +1
Query: 301 LQGLKRSFKETSKALDQVLEKIIQEHE 381
+ GL K+T LD+ LEK++Q+HE
Sbjct: 225 ISGLDGQLKKTGNDLDEFLEKVVQDHE 251
>gb|AAO17011.1| Hypothetical protein [Oryza sativa (japonica cultivar-group)]
Length = 519
Score = 55.5 bits (132), Expect(2) = 1e-09
Identities = 28/74 (37%), Positives = 44/74 (58%), Gaps = 1/74 (1%)
Frame = +3
Query: 108 AAKAGQVVDLSEVMHNLLEDIVYKMVLGCSKVSGF-DLKGLIQRFMYLLGAFNLADHVPC 284
A +AG+ VDL E++ L +D++++ G G +L+ L+Q F L GAFN+ D +P
Sbjct: 169 ARRAGEAVDLGELIFGLTKDVIFRAAFGTRDGGGHGELEVLLQEFSKLFGAFNVGDFIPW 228
Query: 285 LGALDXSGIKTKLQ 326
L LD GI +L+
Sbjct: 229 LAWLDPHGINRRLR 242
Score = 26.9 bits (58), Expect(2) = 1e-09
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +1
Query: 307 GLKRSFKETSKALDQVLEKIIQEHES 384
G+ R + ALD V+++II EH S
Sbjct: 236 GINRRLRAARAALDSVIDRIIDEHVS 261
>ref|NP_680111.1| similar to cytochrome P450-like protein; protein id: At3g48320.1
[Arabidopsis thaliana] gi|13878404|sp|Q9STL2|C71L_ARATH
Cytochrome P450 71A21 gi|7430646|pir||T06710 probable
cytochrome P450 T29H11.160 - Arabidopsis thaliana
gi|4678356|emb|CAB41166.1| cytochrome P450-like protein
[Arabidopsis thaliana]
Length = 490
Score = 54.7 bits (130), Expect(2) = 2e-09
Identities = 32/102 (31%), Positives = 62/102 (60%), Gaps = 1/102 (0%)
Frame = +3
Query: 21 MQLLTISKVDSFESLRKREMLLTVTSLEKAAKAGQVVDLSEVMHNLLEDIVYKMVLGCSK 200
++LL+ V SF ++R+ E+ L + ++K++ V++SE++ +L D++ ++ LG
Sbjct: 133 LRLLSNKMVTSFRNVRQEEISLMMEKIQKSSSLQ--VNVSELLGSLTNDVISRIALGRKY 190
Query: 201 VSGFDLKGLIQRFMYLLGAFNLADHVPCLGALD-XSGIKTKL 323
D K L++R M L+G F++ +VP LG +D SG+ +L
Sbjct: 191 SGETDSKELMKRLMMLMGEFSVGTYVPWLGWIDWISGLDGQL 232
Score = 27.3 bits (59), Expect(2) = 2e-09
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +1
Query: 301 LQGLKRSFKETSKALDQVLEKIIQEH 378
+ GL +T LD+ LEK++Q+H
Sbjct: 225 ISGLDGQLNKTGNDLDEFLEKVVQDH 250
>ref|NP_197878.1| cytochrome P450-like protein; protein id: At5g24960.1 [Arabidopsis
thaliana] gi|13878366|sp|P58045|C71E_ARATH Cytochrome
P450 71A14
Length = 497
Score = 51.2 bits (121), Expect(2) = 6e-09
Identities = 29/96 (30%), Positives = 53/96 (55%), Gaps = 3/96 (3%)
Frame = +3
Query: 21 MQLLTISKVDSFESLRKREMLLTVTSLEKAAKAGQVVDLSEVMHNLLEDIVYKMVLG--- 191
+ LL KV SFE +R+ E+ + +EKA+ ++LSE++ L D+ ++ LG
Sbjct: 134 VNLLNKKKVQSFEKVREEEISEMMERVEKASSDSSPLNLSELLLTLTSDVTSRVSLGRKY 193
Query: 192 CSKVSGFDLKGLIQRFMYLLGAFNLADHVPCLGALD 299
+ S D K +++ L+G F + +++PCL +D
Sbjct: 194 SKEESMSDFKIQMRKITELVGGFPVGEYIPCLAWID 229
Score = 28.9 bits (63), Expect(2) = 6e-09
Identities = 11/26 (42%), Positives = 18/26 (68%)
Frame = +1
Query: 301 LQGLKRSFKETSKALDQVLEKIIQEH 378
L+G+ +E SKA ++EK++QEH
Sbjct: 231 LRGVDEKAEEVSKAFGDLMEKVLQEH 256
Database: nr
Posted date: Apr 1, 2003 2:05 AM
Number of letters in database: 448,689,247
Number of sequences in database: 1,393,205
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 318,668,404
Number of Sequences: 1393205
Number of extensions: 6340583
Number of successful extensions: 18985
Number of sequences better than 10.0: 133
Number of HSP's better than 10.0 without gapping: 17958
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18925
length of database: 448,689,247
effective HSP length: 104
effective length of database: 303,795,927
effective search space used: 7291102248
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
