>KMC004453A_C01 KMC004453A_c01
cttttttactAGTCTAAACAAAGACTAAGAGGATTGCATAAAATTAAAGTGCAAACCAAA
TGAAGTATGACGTTTCTAACAAAGCTCTCAATCTAGTTTATCAACAACAACAACAACAAC
AACAAGCTAGTCCCCCAAGTATAGTTGGTCCCCAAATGGCTCCCGTGACAACAAGAGTAT
CAAGAATCTCTTCATTTGGAGATACTGATTCAAAAGGGTATGGAATGTTGGCTTGTTTTC
TCCAAAGATCCTTGCAGTCAACTGCAGAGATGAAAATTTCAGGAACCTCTTGATAGCATC
AGGACTATAGTCAATCTCTACTCCCCTACAAGACTTGTAAGTAGGCACTGAGGGAGTACG
ATAGGAGAAGTCATACTGAGCGTTGGCATAAAACTCAACAATAAGGTTGGCACATCCTAA
AGCAGTGTGTTTGCAAAGCATTTCACTCCACCTTTTCTTGGCTATGGCCTCCCCAATTTC
TCAAGGGTCCTCATCGGATGCTAATGTCATCGGCCTTT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004453A_C01 KMC004453A_c01
         (518 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_004330.1| pituitary tumor-transforming protein 1-interact...    41  0.009
ref|XP_215370.1| similar to Pituitary tumor-transforming gene 1 ...    37  0.10
pir||T17414 TipC protein - slime mold (Dictyostelium discoideum)...    28  0.35
dbj|BAB13348.1| ALR protein [Equus caballus]                           34  1.2
gb|AAB91452.1| CTG26 alternate open reading frame [Homo sapiens]       33  1.5

>ref|NP_004330.1| pituitary tumor-transforming protein 1-interacting protein
           precursor; chromosome  21 open reading frame 3 protein
           precursor; chromosome 21 open reading frame 1 protein
           precursor; putative surface glycoprotein C21orf1
           precursor; PTTG-binding factor precursor [Homo sapiens]
           gi|1711387|sp|P53801|PTTG_HUMAN Pituitary
           tumor-transforming gene 1 protein-interacting protein
           (Pituitary tumor-transforming gene protein binding
           factor) (PTTG-binding factor) (PBF)
           gi|1107703|emb|CAA90325.1| putative surface glycoprotein
           [Homo sapiens] gi|7717437|emb|CAB90552.1| putative
           surface glycoprotein C21orf1 precursor Accession No.
           Z50022 [Homo sapiens] gi|12653293|gb|AAH00415.1|AAH00415
           pituitary tumor-transforming 1 interacting protein [Homo
           sapiens] gi|15277511|gb|AAH12858.1|AAH12858 Similar to
           pituitary tumor-transforming 1 interacting protein [Homo
           sapiens] gi|17939492|gb|AAH19295.1|AAH19295 pituitary
           tumor-transforming 1 interacting protein [Homo sapiens]
           gi|18088699|gb|AAH20983.1|AAH20983 pituitary
           tumor-transforming 1 interacting protein [Homo sapiens]
           gi|21411022|gb|AAH31097.1| pituitary tumor-transforming
           1 interacting protein [Homo sapiens]
           gi|21707724|gb|AAH34250.1| pituitary tumor-transforming
           1 interacting protein [Homo sapiens]
          Length = 180

 Score = 40.8 bits (94), Expect = 0.009
 Identities = 21/64 (32%), Positives = 31/64 (47%), Gaps = 16/64 (25%)
 Frame = -2

Query: 247 LW--RKQANIPYPFESVSPNEEI--------------LDTLVVTGAIWGPTILGGLACCC 116
           LW    +A + YP  SV P   +               + L++T ++ G T+L G+A CC
Sbjct: 58  LWCNTNKACLDYPVTSVLPPASLCKLSSARWGVCWVNFEALIITMSVVGGTLLLGIAICC 117

Query: 115 CCCC 104
           CCCC
Sbjct: 118 CCCC 121

>ref|XP_215370.1| similar to Pituitary tumor-transforming gene 1 protein-interacting
           protein (Pituitary tumor-transforming gene protein
           binding factor) (PTTG-binding factor) (PBF) [Rattus
           norvegicus]
          Length = 174

 Score = 37.4 bits (85), Expect = 0.10
 Identities = 11/26 (42%), Positives = 19/26 (72%)
 Frame = -2

Query: 181 DTLVVTGAIWGPTILGGLACCCCCCC 104
           + L++T ++ G ++L G+  CCCCCC
Sbjct: 93  EALIITMSVLGGSVLLGITVCCCCCC 118

>pir||T17414 TipC protein - slime mold (Dictyostelium discoideum)
            gi|3420745|gb|AAC31916.1| TipC [Dictyostelium discoideum]
          Length = 3848

 Score = 28.1 bits (61), Expect(2) = 0.35
 Identities = 26/100 (26%), Positives = 44/100 (44%), Gaps = 1/100 (1%)
 Frame = +3

Query: 102  QQQQQQQQASPPSIVGPQMAPVTTRVSRISSFGDTDSKGYGMLACFLQRSLQSTAEMKIS 281
            QQQQQQQQ      +  Q +  ++  S I+S   T    +  L  F+  +++  +   ++
Sbjct: 3015 QQQQQQQQQQQQQSINQQTSLNSSSSSTITSTNSTVI--FEDLRVFINVTIRVKSSKSLT 3072

Query: 282  GTS**HQDYSQSLLPYK-TCK*ALREYDRRSHTERWHKTQ 398
             T     + +  L PY+   K     + R+  TE W K Q
Sbjct: 3073 TTMIILNEQNPELPPYRINNKTRFPLWIRQKKTEIWDKVQ 3112

 Score = 26.2 bits (56), Expect(2) = 0.35
 Identities = 12/23 (52%), Positives = 15/23 (65%)
 Frame = +3

Query: 57   QMKYDVSNKALNLVYQQQQQQQQ 125
            Q+  D SN+    + QQQQQQQQ
Sbjct: 2988 QLNVDSSNQQQQQLQQQQQQQQQ 3010

>dbj|BAB13348.1| ALR protein [Equus caballus]
          Length = 539

 Score = 33.9 bits (76), Expect = 1.2
 Identities = 24/81 (29%), Positives = 37/81 (45%), Gaps = 2/81 (2%)
 Frame = +3

Query: 93  LVYQQQQQQQQASPPSIVGPQ--MAPVTTRVSRISSFGDTDSKGYGMLACFLQRSLQSTA 266
           ++ QQQQQQQQ   P ++GPQ     V    SR+ S      +G G+    L  + Q   
Sbjct: 228 VLQQQQQQQQQQHHPGVLGPQGPHRQVLLNQSRVLSSPQLAQQGQGLTGHRLVTAQQQQQ 287

Query: 267 EMKISGTS**HQDYSQSLLPY 329
           + +  G+        Q L+P+
Sbjct: 288 QHQQQGSVAGLSHLQQGLMPH 308

>gb|AAB91452.1| CTG26 alternate open reading frame [Homo sapiens]
          Length = 124

 Score = 33.5 bits (75), Expect = 1.5
 Identities = 9/13 (69%), Positives = 11/13 (84%)
 Frame = -2

Query: 142 ILGGLACCCCCCC 104
           +L G+ CCCCCCC
Sbjct: 96  LLRGMGCCCCCCC 108

 Score = 30.8 bits (68), Expect = 9.8
 Identities = 8/10 (80%), Positives = 8/10 (80%)
 Frame = -2

Query: 133 GLACCCCCCC 104
           G  CCCCCCC
Sbjct: 101 GCCCCCCCCC 110

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 429,599,878
Number of Sequences: 1393205
Number of extensions: 9189304
Number of successful extensions: 92797
Number of sequences better than 10.0: 64
Number of HSP's better than 10.0 without gapping: 35891
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 85567
length of database: 448,689,247
effective HSP length: 115
effective length of database: 288,470,672
effective search space used: 16442828304
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MPD048d08_f AV773252 1 519 2 MR041b04_f BP079143 11 407

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