>KMC004442A_C01 KMC004442A_c01
ttttttttTTAGTTCTGATCAATCATTCAATTAATATTAATAGGATAAAAAGCTGGGAAA
CAAGGCTCTTCCAAGTGAGCCAACACACACAACAGAACAAAGCAACTACAGAAATAAACA
AGAAACAACATAAGAAATGGAAAAACAGATAACAGACACAACAAACAAGGTAGAAAGCAA
AGACAACTGATAACATGTTGTCCCTAAACTAAGTTTCTCCTATGTTGATATTCAACAAAC
ATATCCATTGCTAAAGTGTCCCGAAACGTTGTCCATGCCTAAGATACTTGAATAGTTGTG
ACCTCATTTGCAACATTACTTGTATATCCTGGGCCATTTGTATATCCTGCGTCGACCAGA
AAGTATTTACCATTCGGAATTTGAAGGTGGTTTTGACGACGTAAAGCATCTCGCAATACT
CGTGAATCTCCTGCCGAGCCTTCCCAACCAGGTAACACGTAAATAAACTTTAAATCTGGA
CCACAAACCCCTAACACATTCGTAGAGATGTCACCCTTTCTATTACGGTATCTAGGTTTA
TCTTCTGCTG

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004442A_C01 KMC004442A_c01
         (550 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAL11884.1|AF412282_1 putative transposase [Zea mays]              112  3e-24
ref|NP_198077.1| putative protein; protein id: At5g27250.1 [Arab...    94  8e-19
ref|NP_190050.1| putative protein; protein id: At3g44640.1 [Arab...    90  2e-17
pir||T01797 hypothetical protein A_TM021B04.9 - Arabidopsis thal...    83  2e-15
dbj|BAC16200.1| transposase-like [Oryza sativa (japonica cultiva...    80  1e-14

>gb|AAL11884.1|AF412282_1 putative transposase [Zea mays]
          Length = 298

 Score =  112 bits (280), Expect = 3e-24
 Identities = 51/73 (69%), Positives = 60/73 (81%)
 Frame = -3

Query: 542 DKPRYRNRKGDISTNVLGVCGPDLKFIYVLPGWEGSAGDSRVLRDALRRQNHLQIPNGKY 363
           D+ RYRNRK  I+TNVLGVC   +KF+YVL GWEGSA DSRVLRDA+ R +   IP+GKY
Sbjct: 185 DQGRYRNRKQQITTNVLGVCDRHMKFVYVLAGWEGSASDSRVLRDAMSRDDAFAIPSGKY 244

Query: 362 FLVDAGYTNGPGY 324
           +LVDAGYTNGPG+
Sbjct: 245 YLVDAGYTNGPGF 257

>ref|NP_198077.1| putative protein; protein id: At5g27250.1 [Arabidopsis thaliana]
          Length = 348

 Score = 94.4 bits (233), Expect = 8e-19
 Identities = 46/70 (65%), Positives = 53/70 (75%), Gaps = 1/70 (1%)
 Frame = -3

Query: 542 DKPRYRNRKGDISTNVLGVCGPDLKFIYVLPGWEGSAGDSRVLRDAL-RRQNHLQIPNGK 366
           +K  YRNRKG IS NVL  C  DL+FIYVL GWEGSA DS+VL+DAL RR N LQ+P GK
Sbjct: 130 EKASYRNRKGVISQNVLAACNFDLEFIYVLSGWEGSAHDSKVLQDALTRRTNRLQVPEGK 189

Query: 365 YFLVDAGYTN 336
           Y+L D G+ N
Sbjct: 190 YYLADCGFPN 199

>ref|NP_190050.1| putative protein; protein id: At3g44640.1 [Arabidopsis thaliana]
           gi|11358076|pir||T47439 hypothetical protein T18B22.40 -
           Arabidopsis thaliana gi|6967112|emb|CAB72466.1| putative
           protein [Arabidopsis thaliana]
          Length = 377

 Score = 89.7 bits (221), Expect = 2e-17
 Identities = 43/70 (61%), Positives = 51/70 (72%), Gaps = 1/70 (1%)
 Frame = -3

Query: 542 DKPRYRNRKGDISTNVLGVCGPDLKFIYVLPGWEGSAGDSRVLRDAL-RRQNHLQIPNGK 366
           D   +RNRKG IS NVL  C  DL+FIY+L GW+GSA DS+VL DAL R  N LQ+P GK
Sbjct: 163 DAASFRNRKGFISQNVLAACNFDLQFIYILSGWKGSAHDSKVLNDALTRNTNRLQVPEGK 222

Query: 365 YFLVDAGYTN 336
           ++LVD GY N
Sbjct: 223 FYLVDCGYAN 232

>pir||T01797 hypothetical protein A_TM021B04.9 - Arabidopsis thaliana
            gi|2191187|gb|AAB61072.1| contains similarity to a
            DNAJ-like domain [Arabidopsis thaliana]
          Length = 1609

 Score = 82.8 bits (203), Expect = 2e-15
 Identities = 44/71 (61%), Positives = 49/71 (68%), Gaps = 1/71 (1%)
 Frame = -3

Query: 542  DKPRYRNRKGDISTNVLGVCGPDLKFIYVLPGWEGSAGDSRVLRDAL-RRQNHLQIPNGK 366
            +K  YRNRKG IS NVL  C  DL+FIYVL GWEGSA DS+VL+DAL RR N LQ+P G 
Sbjct: 1238 EKASYRNRKGVISQNVLAACNFDLEFIYVLSGWEGSAHDSKVLQDALTRRTNRLQVPEGN 1297

Query: 365  YFLVDAGYTNG 333
               V  G T G
Sbjct: 1298 RLQVLKGITMG 1308

>dbj|BAC16200.1| transposase-like [Oryza sativa (japonica cultivar-group)]
          Length = 395

 Score = 80.5 bits (197), Expect = 1e-14
 Identities = 41/66 (62%), Positives = 49/66 (74%), Gaps = 1/66 (1%)
 Frame = -3

Query: 530 YRNRKGDISTNVLGVCGPDLKFIYVLPGWEGSAGDSRVLRDALRRQNH-LQIPNGKYFLV 354
           YRNRK  IS NV+  C  DLKF++V  GWEGSA D+RVL+DAL   NH  ++P GK+FLV
Sbjct: 185 YRNRKQTISQNVMVACDFDLKFVHVHAGWEGSASDARVLQDAL---NHGFEVPPGKFFLV 241

Query: 353 DAGYTN 336
           DAGY N
Sbjct: 242 DAGYAN 247

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 460,173,850
Number of Sequences: 1393205
Number of extensions: 9820543
Number of successful extensions: 31120
Number of sequences better than 10.0: 78
Number of HSP's better than 10.0 without gapping: 28957
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30946
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 18947112822
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MFB023h04_f BP035694 1 127 2 MPD087b02_f AV775693 8 519 3 MR043d04_f BP079322 11 398 4 SPD038c03_f BP047003 11 556 5 MR039h07_f BP079052 11 348 6 SPDL003d07_f BP052173 14 389 7 MR063e04_f BP080839 15 355 8 MWL039h03_f AV769236 15 456 9 MF087b03_f BP032857 18 451 10 SPD043g10_f BP047456 20 539 11 SPDL040a11_f BP054494 26 533 12 MFB096e10_f BP041008 32 553

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