>KMC004437A_C01 KMC004437A_c01
AAATAATCAATTGTGAGATTCCATAACTAGATTTCACATAATTACTCAACAAAAATCACT
TTCCCATAAAGTATCTAAAGATAAATCACTTCACTTTCAATTCACATTAACTCACTTTTA
TCATAATCAATTATAACAAAACCAATTCTATCCAATATTCATTCTCACAAGCATTGATCC
AAACACGCGCCTGGTGAGCTTCTTCCAAGAAACAAAAGAATCATTCATGGGCAACCCGAA
TTACTTGCTTACCAACATTCTTGCCAAGGAAAAGCCCAGCCAAAGCAGCTGGAGCACTTT
CCAAGCCTTCATTCATGTCTTCAATATACACAATCTTTCCTTGCTTGTAATAACTTGAAA
CAAGCTCCAAAAACTGAGGATATAAGTGCAAGTAATCATGTTGCAGAAAACCCTGCATTT
TGATGCGCCTTATTATGAGATTGGATAAGTTGTATATCCCTTGGGGATTAGAAAGGCTGT
TCTGAGACACCATTCCACAAACTGCAACCCTGCCATGAATCCTCATGTTAAGGAGTGCAG
CATCAAGCATGTCGCCACCCA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004437A_C01 KMC004437A_c01
         (561 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

gb|AAK59836.1| At1g65560/F5I14_32 [Arabidopsis thaliana] gi|1849...   156  1e-37
pir||E96680 hypothetical protein F5I14.9 [imported] - Arabidopsi...   156  1e-37
ref|NP_176734.1| allyl alcohol dehydrogenase, putative; protein ...   156  1e-37
dbj|BAB90185.1| putative allyl alcohol dehydrogenase [Oryza sati...   154  5e-37
gb|AAM63201.1| quinone oxidoreductase-like protein [Arabidopsis ...   144  7e-34

>gb|AAK59836.1| At1g65560/F5I14_32 [Arabidopsis thaliana]
           gi|18491115|gb|AAL69524.1| At1g65560/F5I14_32
           [Arabidopsis thaliana]
          Length = 209

 Score =  156 bits (395), Expect = 1e-37
 Identities = 75/113 (66%), Positives = 95/113 (83%), Gaps = 1/113 (0%)
 Frame = -3

Query: 559 GGDMLDAALLNMRIHGRVAVCGMVSQNSLS-NPQGIYNLSNLIIRRIKMQGFLQHDYLHL 383
           GG MLDAALLNM++ GR+A+CGMVS  SLS + QGI NL + I +R++++GFLQ DYLH+
Sbjct: 97  GGSMLDAALLNMKVRGRIALCGMVSLQSLSTSSQGIKNLYSAIYKRLRLEGFLQSDYLHI 156

Query: 382 YPQFLELVSSYYKQGKIVYIEDMNEGLESAPAALAGLFLGKNVGKQVIRVAHE 224
           +PQFLE V  YYK+GKIVY+ED++EGL+ APAAL GLF GKN+GKQV+RVA E
Sbjct: 157 FPQFLENVKRYYKEGKIVYVEDISEGLDLAPAALVGLFSGKNIGKQVVRVAKE 209

>pir||E96680 hypothetical protein F5I14.9 [imported] - Arabidopsis thaliana
           gi|2190553|gb|AAB60917.1| Strong similarity to
           Arabidopsis zeta-crystallin-like protein (gb|Z49268).
           [Arabidopsis thaliana]
          Length = 432

 Score =  156 bits (395), Expect = 1e-37
 Identities = 75/113 (66%), Positives = 95/113 (83%), Gaps = 1/113 (0%)
 Frame = -3

Query: 559 GGDMLDAALLNMRIHGRVAVCGMVSQNSLS-NPQGIYNLSNLIIRRIKMQGFLQHDYLHL 383
           GG MLDAALLNM++ GR+A+CGMVS  SLS + QGI NL + I +R++++GFLQ DYLH+
Sbjct: 320 GGSMLDAALLNMKVRGRIALCGMVSLQSLSTSSQGIKNLYSAIYKRLRLEGFLQSDYLHI 379

Query: 382 YPQFLELVSSYYKQGKIVYIEDMNEGLESAPAALAGLFLGKNVGKQVIRVAHE 224
           +PQFLE V  YYK+GKIVY+ED++EGL+ APAAL GLF GKN+GKQV+RVA E
Sbjct: 380 FPQFLENVKRYYKEGKIVYVEDISEGLDLAPAALVGLFSGKNIGKQVVRVAKE 432

>ref|NP_176734.1| allyl alcohol dehydrogenase, putative; protein id: At1g65560.1,
           supported by cDNA: gi_14335113 [Arabidopsis thaliana]
          Length = 350

 Score =  156 bits (395), Expect = 1e-37
 Identities = 75/113 (66%), Positives = 95/113 (83%), Gaps = 1/113 (0%)
 Frame = -3

Query: 559 GGDMLDAALLNMRIHGRVAVCGMVSQNSLS-NPQGIYNLSNLIIRRIKMQGFLQHDYLHL 383
           GG MLDAALLNM++ GR+A+CGMVS  SLS + QGI NL + I +R++++GFLQ DYLH+
Sbjct: 238 GGSMLDAALLNMKVRGRIALCGMVSLQSLSTSSQGIKNLYSAIYKRLRLEGFLQSDYLHI 297

Query: 382 YPQFLELVSSYYKQGKIVYIEDMNEGLESAPAALAGLFLGKNVGKQVIRVAHE 224
           +PQFLE V  YYK+GKIVY+ED++EGL+ APAAL GLF GKN+GKQV+RVA E
Sbjct: 298 FPQFLENVKRYYKEGKIVYVEDISEGLDLAPAALVGLFSGKNIGKQVVRVAKE 350

>dbj|BAB90185.1| putative allyl alcohol dehydrogenase [Oryza sativa (japonica
           cultivar-group)]
          Length = 359

 Score =  154 bits (390), Expect = 5e-37
 Identities = 71/112 (63%), Positives = 95/112 (84%)
 Frame = -3

Query: 559 GGDMLDAALLNMRIHGRVAVCGMVSQNSLSNPQGIYNLSNLIIRRIKMQGFLQHDYLHLY 380
           GG MLDA LLNMR HGR+AVCGMVSQN+L++P GI+N+  L+ +RI+MQGF+Q D+LH++
Sbjct: 242 GGPMLDAVLLNMRTHGRIAVCGMVSQNALTDPVGIHNIFCLVPKRIRMQGFIQSDHLHMF 301

Query: 379 PQFLELVSSYYKQGKIVYIEDMNEGLESAPAALAGLFLGKNVGKQVIRVAHE 224
           PQF+  ++ +Y+ GKIVY+EDM+ GLE+APAAL GLF GKNVGK+V+ V+ E
Sbjct: 302 PQFVSDMAKHYRDGKIVYVEDMSIGLENAPAALVGLFSGKNVGKKVVCVSQE 353

>gb|AAM63201.1| quinone oxidoreductase-like protein [Arabidopsis thaliana]
          Length = 343

 Score =  144 bits (363), Expect = 7e-34
 Identities = 68/112 (60%), Positives = 87/112 (76%)
 Frame = -3

Query: 559 GGDMLDAALLNMRIHGRVAVCGMVSQNSLSNPQGIYNLSNLIIRRIKMQGFLQHDYLHLY 380
           GG MLDA LLNM  HGR+AVCGM+SQ +L N +G++NLSN+I +RI++QGF+  D+   Y
Sbjct: 232 GGKMLDAVLLNMNPHGRIAVCGMISQYNLENQEGVHNLSNIIYKRIRIQGFVVSDFYDKY 291

Query: 379 PQFLELVSSYYKQGKIVYIEDMNEGLESAPAALAGLFLGKNVGKQVIRVAHE 224
           P+FLELV    K+GKI Y+ED+ +GLE AP AL GLF GKNVGKQV+ +A E
Sbjct: 292 PKFLELVLPRIKEGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVIARE 343

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 476,649,770
Number of Sequences: 1393205
Number of extensions: 9890451
Number of successful extensions: 22763
Number of sequences better than 10.0: 125
Number of HSP's better than 10.0 without gapping: 22185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22701
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 20095422690
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MF060f12_f BP031480 1 474 2 MWM070g04_f AV765832 24 358 3 MR039b01_f BP078993 67 442 4 MFB046a06_f BP037326 100 584

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