>KMC004396A_C01 KMC004396A_c01
AGCCGAGAGAACAAATGTTAAAAATTCATTGAGGACAGAGGTACAGACAAATTCACATTA
AATAACTTGAATTTAAGTTTTTTCAACTTTGATCATATATCGTATGATATTATTTAGTGT
TACAGTAATGCTATTTCGAGCCTTTAATCGAAGTCCCTAAAAAACTCTCATGATGAACTA
GCACTGTTCTGATTCCAGGATTGGATCTCCTTTTTTTCAGCTTAGCTTATTTGAGCTTTC
TTCATGCTCCAAGCGCTCAATCTCGCGATCTTGACATCAGCAGTTCCATTGTTAAAAGCG
TAAATTTGTGCTTTATCATTTATGGCCAATGTGGGATAAACTCTGGCTGTGATGCAAGCC
TTTCCTTCTCCTCCAAAACTCTCCACAACAGAGTGATCAATCAAGGCTCTCAATGACAGC
TTCTCATAAATAATGTTCACATCAACAAAAGTTCCATAAGTGGTCCTATCATTTTCTTTA
TTCA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004396A_C01 KMC004396A_c01
         (484 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||T12095 beta-fructofuranosidase (EC 3.2.1.26), cell wall - f...   147  6e-35
pir||T06380 beta-fructofuranosidase (EC 3.2.1.26) - garden pea g...   140  1e-32
emb|CAD19322.1| exocellular acid invertase 1 [Beta vulgaris]          125  2e-28
emb|CAB95010.1| invertase [Beta vulgaris]                             125  2e-28
emb|CAA57389.1| beta-fructofuranosidase [Chenopodium rubrum]          122  3e-27

>pir||T12095 beta-fructofuranosidase (EC 3.2.1.26), cell wall - fava bean
           gi|861157|emb|CAA84527.1| cell wall invertase II;
           beta-furanofructosidase [Vicia faba]
          Length = 581

 Score =  147 bits (371), Expect = 6e-35
 Identities = 68/86 (79%), Positives = 80/86 (92%)
 Frame = -3

Query: 482 NKENDRTTYGTFVDVNIIYEKLSLRALIDHSVVESFGGEGKACITARVYPTLAINDKAQI 303
           NK+ND T+YGTF+DV++++EKLSLR LIDHSVVESFGGEGKACITARVYPTLA+NDKA I
Sbjct: 493 NKDNDLTSYGTFIDVDVLHEKLSLRTLIDHSVVESFGGEGKACITARVYPTLAVNDKALI 552

Query: 302 YAFNNGTADVKIARLSAWSMKKAQIS 225
           YAFNNGT DVKI  L+AWSMKKA+++
Sbjct: 553 YAFNNGTTDVKITSLNAWSMKKARLN 578

>pir||T06380 beta-fructofuranosidase (EC 3.2.1.26) - garden pea
           gi|3152880|gb|AAC17166.1| cell wall invertase;
           beta-fructofuranosidase [Pisum sativum]
          Length = 582

 Score =  140 bits (352), Expect = 1e-32
 Identities = 66/84 (78%), Positives = 77/84 (91%)
 Frame = -3

Query: 482 NKENDRTTYGTFVDVNIIYEKLSLRALIDHSVVESFGGEGKACITARVYPTLAINDKAQI 303
           NK+ND T+YGTFVDV+ ++EKLSLR LIDHSVVESFGGEG+AC+TARVYPTLAI+DKA +
Sbjct: 490 NKDNDMTSYGTFVDVDPLHEKLSLRTLIDHSVVESFGGEGRACVTARVYPTLAIHDKAML 549

Query: 302 YAFNNGTADVKIARLSAWSMKKAQ 231
           YAFNNGT+ VKI  LSAWSMKKA+
Sbjct: 550 YAFNNGTSAVKITSLSAWSMKKAK 573

>emb|CAD19322.1| exocellular acid invertase 1 [Beta vulgaris]
          Length = 567

 Score =  125 bits (314), Expect = 2e-28
 Identities = 62/86 (72%), Positives = 71/86 (82%)
 Frame = -3

Query: 482 NKENDRTTYGTFVDVNIIYEKLSLRALIDHSVVESFGGEGKACITARVYPTLAINDKAQI 303
           N  ND+TTYGTFVDVN I E LSLR LIDHSVVESFG +GK  ITARVYPTLAIN+KA +
Sbjct: 482 NPTNDKTTYGTFVDVNPIREGLSLRVLIDHSVVESFGAKGKNVITARVYPTLAINEKAHL 541

Query: 302 YAFNNGTADVKIARLSAWSMKKAQIS 225
           Y FN GT++V+I  L+AWSMKKA I+
Sbjct: 542 YVFNRGTSNVEITGLTAWSMKKANIA 567

>emb|CAB95010.1| invertase [Beta vulgaris]
          Length = 501

 Score =  125 bits (314), Expect = 2e-28
 Identities = 62/86 (72%), Positives = 71/86 (82%)
 Frame = -3

Query: 482 NKENDRTTYGTFVDVNIIYEKLSLRALIDHSVVESFGGEGKACITARVYPTLAINDKAQI 303
           N  ND+TTYGTFVDVN I E LSLR LIDHSVVESFG +GK  ITARVYPTLAIN+KA +
Sbjct: 416 NPTNDKTTYGTFVDVNPIREGLSLRVLIDHSVVESFGAKGKNVITARVYPTLAINEKAHL 475

Query: 302 YAFNNGTADVKIARLSAWSMKKAQIS 225
           Y FN GT++V+I  L+AWSMKKA I+
Sbjct: 476 YVFNRGTSNVEITGLTAWSMKKANIA 501

>emb|CAA57389.1| beta-fructofuranosidase [Chenopodium rubrum]
          Length = 573

 Score =  122 bits (305), Expect = 3e-27
 Identities = 60/87 (68%), Positives = 71/87 (80%), Gaps = 1/87 (1%)
 Frame = -3

Query: 482 NKENDRTTYGTFVDVNIIYEKLSLRALIDHSVVESFGGEGKACITARVYPTLAINDKA-Q 306
           N  ND+TTYG+FVDVN + E LSLR LIDHSVVESFG + K C+TARVYPTLAIN+KA  
Sbjct: 487 NPTNDKTTYGSFVDVNPVREDLSLRVLIDHSVVESFGAKRKECVTARVYPTLAINEKACN 546

Query: 305 IYAFNNGTADVKIARLSAWSMKKAQIS 225
           +Y FNNG +DV+I  L+AWSMKKA I+
Sbjct: 547 LYVFNNGKSDVEITGLTAWSMKKASIA 573

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 341,986,412
Number of Sequences: 1393205
Number of extensions: 6132420
Number of successful extensions: 13424
Number of sequences better than 10.0: 220
Number of HSP's better than 10.0 without gapping: 13208
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 13412
length of database: 448,689,247
effective HSP length: 113
effective length of database: 291,257,082
effective search space used: 13689082854
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR034h12_f BP078665 1 371 2 MWL031f10_f AV769089 1 484 3 MWM079d06_f AV766004 8 315 4 MR099c05_f BP083570 71 215

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