>KMC004373A_C01 KMC004373A_c01
gAAAGAAAACTCATGATAGGTAGTTCATTATAAAAATGGCTAAGATCAGGTTACTTTATG
AAACTGTGCCAACACATGGCACTAACATAACCATCACTAACATCACACAATGCAACAATC
AAATAGAACTAATATTATATTTTTTGAATCACACATCAAATTTATACATGCATATGAGAG
CATCTGTACCAATCAAAGGCACCAAAAGCTTAGTAAACAATAAACACACATAACAAAACG
TGCTATATTGACTCCATGGCCTTCATAGTTTCAAATCTGGGCTCTTGGGCCTGGAACTTG
AGGCCAGCATAGAGCTTCATGTAATCATCAAAAACAAATTTGGGGTAGAGTTGGCTTGTG
TCATCATTTTCCTTCACCAAGGCTGGTGCTGGAGAGATCATTGCATCATTGCCTGGGTTG
TAAAATGAGGCTATGGACATTCT

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004373A_C01 KMC004373A_c01
         (443 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

pir||T10813 1-aminocyclopropane-1-carboxylate oxidase (EC 1.4.3....   118  2e-26
sp|P54847|ACC3_CUCME 1-aminocyclopropane-1-carboxylate oxidase 3...   110  4e-24
gb|AAC67233.1| ACC oxidase 2 [Cucumis sativus]                        110  5e-24
dbj|BAA21541.1| 1-aminocyclopropane-1-carboxylic acid oxidase [A...   109  7e-24
gb|AAD28196.1|AF115261_1 1-aminocyclopropane-1-carboxylate oxida...   108  1e-23

>pir||T10813 1-aminocyclopropane-1-carboxylate oxidase (EC 1.4.3.-) ACO1 - mung
           bean gi|458338|gb|AAC48921.1|
           1-aminocylopropane-1-carboxylate oxidase homolog [Vigna
           radiata] gi|12802439|gb|AAK07883.1|AF315316_1 ACC
           oxidase [Vigna radiata] gi|1093118|prf||2102361A
           aminocyclopropane carboxylate oxidase
          Length = 317

 Score =  118 bits (295), Expect = 2e-26
 Identities = 54/66 (81%), Positives = 64/66 (96%)
 Frame = -1

Query: 443 RMSIASFYNPGNDAMISPAPALVKENDDTSQLYPKFVFDDYMKLYAGLKFQAQEPRFETM 264
           RMS+ASFYNPG+DA+ISPAPALVKE+D+TSQ+YPKFVF+DYMKLYAGLKFQA+EPRFE M
Sbjct: 244 RMSLASFYNPGDDAVISPAPALVKESDETSQVYPKFVFNDYMKLYAGLKFQAKEPRFEAM 303

Query: 263 KAMESI 246
           KA+ S+
Sbjct: 304 KAVSSV 309

>sp|P54847|ACC3_CUCME 1-aminocyclopropane-1-carboxylate oxidase 3 (ACC oxidase 3)
           (Ethylene-forming enzyme) (EFE) gi|2129504|pir||S66176
           ACC oxidase (clone ACO3) oxidase - muskmelon
           gi|1183900|emb|CAA64799.1| ACC oxidase [Cucumis melo]
          Length = 320

 Score =  110 bits (275), Expect = 4e-24
 Identities = 52/65 (80%), Positives = 58/65 (89%)
 Frame = -1

Query: 443 RMSIASFYNPGNDAMISPAPALVKENDDTSQLYPKFVFDDYMKLYAGLKFQAQEPRFETM 264
           RMSIASFYNPGNDA+I PAPALV+   + ++LYPKFVFDDYMKLY GLKFQA+EPRFE M
Sbjct: 245 RMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYVGLKFQAKEPRFEAM 304

Query: 263 KAMES 249
           KAMES
Sbjct: 305 KAMES 309

>gb|AAC67233.1| ACC oxidase 2 [Cucumis sativus]
          Length = 318

 Score =  110 bits (274), Expect = 5e-24
 Identities = 52/65 (80%), Positives = 58/65 (89%)
 Frame = -1

Query: 443 RMSIASFYNPGNDAMISPAPALVKENDDTSQLYPKFVFDDYMKLYAGLKFQAQEPRFETM 264
           RMSIASFYNPGNDA+I PAPALV+   + ++LYPKFVFDDYMKLY GLKFQA+EPRFE M
Sbjct: 243 RMSIASFYNPGNDAVIYPAPALVEGEQEKTKLYPKFVFDDYMKLYMGLKFQAKEPRFEAM 302

Query: 263 KAMES 249
           KAMES
Sbjct: 303 KAMES 307

>dbj|BAA21541.1| 1-aminocyclopropane-1-carboxylic acid oxidase [Actinidia deliciosa]
          Length = 317

 Score =  109 bits (273), Expect = 7e-24
 Identities = 54/66 (81%), Positives = 59/66 (88%), Gaps = 1/66 (1%)
 Frame = -1

Query: 443 RMSIASFYNPGNDAMISPAPALV-KENDDTSQLYPKFVFDDYMKLYAGLKFQAQEPRFET 267
           RMSIASFYNPG+DA+I PAPALV KE D   Q+YPKFVF+DYMKLYAGLKFQA+EPRFE 
Sbjct: 242 RMSIASFYNPGSDAVIYPAPALVDKEEDQQKQVYPKFVFEDYMKLYAGLKFQAKEPRFEA 301

Query: 266 MKAMES 249
           MKAMES
Sbjct: 302 MKAMES 307

>gb|AAD28196.1|AF115261_1 1-aminocyclopropane-1-carboxylate oxidase [Trifolium repens]
          Length = 292

 Score =  108 bits (270), Expect = 1e-23
 Identities = 51/67 (76%), Positives = 60/67 (89%), Gaps = 1/67 (1%)
 Frame = -1

Query: 443 RMSIASFYNPGNDAMISPAPALVKENDDTSQLYPKFVFDDYMKLYAGLKFQAQEPRFET- 267
           RMS+ASFYNP +DA+ISPAP L+KEN+ TS++YPKFVFDDYMKLY GLKFQA+EPRFE  
Sbjct: 218 RMSLASFYNPSDDAIISPAPTLLKENETTSEIYPKFVFDDYMKLYMGLKFQAKEPRFEAM 277

Query: 266 MKAMESI 246
           MKAM S+
Sbjct: 278 MKAMSSV 284

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 368,391,379
Number of Sequences: 1393205
Number of extensions: 7387448
Number of successful extensions: 20489
Number of sequences better than 10.0: 165
Number of HSP's better than 10.0 without gapping: 18669
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 20078
length of database: 448,689,247
effective HSP length: 123
effective length of database: 277,325,032
effective search space used: 6655800768
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR098e08_f BP083515 1 443 2 MR031h05_f BP078420 2 389

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