>KMC004366A_C01 KMC004366A_c01
GGAATCAGTGATGAATCTTCTTTTAATAAATAAAGAAGTGATAAATGAAAGTTGTGAATT
ACAATTCATCCTCAAAGCTATAACAAAATACAATGAACATTGACTTGTGCTTTCCACATA
AAACTGCACAATTTTTAACATCCTTTTCAAATCCTAACTTTACTATATTGAACAAGTTAG
CATAGAGAAGATATCTTAGACTTCCCATTCCCTCATACACAAAAAGTGGAACTTTACCTG
AATTACACAGCCCTGTGTCCCTGGGATGATAATTAGAGCTTCCAGAATGTCTTGGTAGCA
ACTAGAATTTTTTCCCTGCCATGTGAGTCCGGTTCTTTATCATGAATGATAGGCTTCCAC
CTAACTGCACGAGCAATGGAAGCTACTTTGTTAATTACTTCAGGGGTATCCCTCACCACA
ACAGTTCCCTCCGGGCGCAACATTCGATCAATTTCCACCATCAAATCCACAATACTACAT
CTGCTTTTACCAGAATCTGGATCTTTTACTAGTGAATCAATGCTGTCCACATGGATCAGA
TCATAACTA

BLASTX 2.2.2 [Dec-14-2001]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= KMC004366A_C01 KMC004366A_c01
         (549 letters)

Database: nr 
           1,393,205 sequences; 448,689,247 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

ref|NP_567184.1| unknown protein; protein id: At4g00740.1, suppo...   138  5e-32
pir||B85010 hypothetical protein AT4g00740 [imported] - Arabidop...   110  1e-23
gb|AAK84446.1|AC087192_7 unknown protein [Oryza sativa (japonica...    88  6e-17
dbj|BAC22226.1| hypothetical protein~similar to Oryza sativa chr...    86  2e-16
dbj|BAA83350.1| Similar to Arabidopsis thaliana BAC F15P23 (AF12...    81  7e-15

>ref|NP_567184.1| unknown protein; protein id: At4g00740.1, supported by cDNA:
           gi_16648930, supported by cDNA: gi_16649086 [Arabidopsis
           thaliana] gi|16648931|gb|AAL24317.1| Unknown protein
           [Arabidopsis thaliana] gi|16649087|gb|AAL24395.1|
           Unknown protein [Arabidopsis thaliana]
           gi|23197886|gb|AAN15470.1| Unknown protein [Arabidopsis
           thaliana]
          Length = 600

 Score =  138 bits (347), Expect = 5e-32
 Identities = 63/91 (69%), Positives = 78/91 (85%)
 Frame = -2

Query: 548 SYDLIHVDSIDSLVKDPDSGKSRCSIVDLMVEIDRMLRPEGTVVVRDTPEVINKVASIAR 369
           +YD IHV  I+SL+K  DS KSRCS+VDLMVE+DR+LRPEG VV+RD+PEV++KVA +A 
Sbjct: 505 TYDFIHVSGIESLIKRQDSSKSRCSLVDLMVEMDRILRPEGKVVIRDSPEVLDKVARMAH 564

Query: 368 AVRWKPIIHDKEPDSHGREKILVATKTFWKL 276
           AVRW   IH+KEP+SHGREKIL+ATK+ WKL
Sbjct: 565 AVRWSSSIHEKEPESHGREKILIATKSLWKL 595

>pir||B85010 hypothetical protein AT4g00740 [imported] - Arabidopsis thaliana
           gi|4325338|gb|AAD17338.1| F15P23.2 gene product
           [Arabidopsis thaliana] gi|7267413|emb|CAB80883.1|
           predicted protein of unknown function [Arabidopsis
           thaliana]
          Length = 590

 Score =  110 bits (274), Expect = 1e-23
 Identities = 49/68 (72%), Positives = 61/68 (89%)
 Frame = -2

Query: 479 CSIVDLMVEIDRMLRPEGTVVVRDTPEVINKVASIARAVRWKPIIHDKEPDSHGREKILV 300
           CS+VDLMVE+DR+LRPEG VV+RD+PEV++KVA +A AVRW   IH+KEP+SHGREKIL+
Sbjct: 518 CSLVDLMVEMDRILRPEGKVVIRDSPEVLDKVARMAHAVRWSSSIHEKEPESHGREKILI 577

Query: 299 ATKTFWKL 276
           ATK+ WKL
Sbjct: 578 ATKSLWKL 585

>gb|AAK84446.1|AC087192_7 unknown protein [Oryza sativa (japonica cultivar-group)]
          Length = 634

 Score = 88.2 bits (217), Expect = 6e-17
 Identities = 40/89 (44%), Positives = 62/89 (68%)
 Frame = -2

Query: 548 SYDLIHVDSIDSLVKDPDSGKSRCSIVDLMVEIDRMLRPEGTVVVRDTPEVINKVASIAR 369
           +YDL+H D++ S+ +D      RC I ++++E+DR+LRPEGT ++RDT +V+ KV +IA+
Sbjct: 544 TYDLLHADNLFSIYQD------RCDITNILLEMDRILRPEGTAIIRDTVDVLTKVQAIAK 597

Query: 368 AVRWKPIIHDKEPDSHGREKILVATKTFW 282
            +RW+  I D E      EK+LVA KT+W
Sbjct: 598 RMRWESRILDHEDGPFNPEKVLVAVKTYW 626

>dbj|BAC22226.1| hypothetical protein~similar to Oryza sativa chromosome 10,
           OSJNBa0005K07.2 [Oryza sativa (japonica cultivar-group)]
          Length = 663

 Score = 86.3 bits (212), Expect = 2e-16
 Identities = 43/89 (48%), Positives = 56/89 (62%)
 Frame = -2

Query: 548 SYDLIHVDSIDSLVKDPDSGKSRCSIVDLMVEIDRMLRPEGTVVVRDTPEVINKVASIAR 369
           +YD IH D I S  +D      RC +  +++E+DR+LRPEGTV+ RDT EV+ K+ SI  
Sbjct: 573 TYDFIHADKIFSFYQD------RCDVTYILLEMDRILRPEGTVIFRDTVEVLVKIQSITE 626

Query: 368 AVRWKPIIHDKEPDSHGREKILVATKTFW 282
            +RWK  I D E      EKILVA KT+W
Sbjct: 627 GMRWKSQIMDHESGPFNPEKILVAVKTYW 655

>dbj|BAA83350.1| Similar to Arabidopsis thaliana BAC F15P23 (AF128392) [Oryza sativa
           (japonica cultivar-group)] gi|25553569|dbj|BAC24840.1|
           OSJNBa0038F22.19 [Oryza sativa (japonica
           cultivar-group)]
          Length = 618

 Score = 81.3 bits (199), Expect = 7e-15
 Identities = 40/89 (44%), Positives = 60/89 (66%)
 Frame = -2

Query: 548 SYDLIHVDSIDSLVKDPDSGKSRCSIVDLMVEIDRMLRPEGTVVVRDTPEVINKVASIAR 369
           +YDLIH  S+ ++ KD      RC + D+++E+DR+LRPEGTV+ RD  +V+ K+ +IA 
Sbjct: 534 TYDLIHAYSLFTMYKD------RCEMEDILLEMDRVLRPEGTVIFRDDVDVLVKIKNIAD 587

Query: 368 AVRWKPIIHDKEPDSHGREKILVATKTFW 282
            +RW+  I D E     REKILV+ K++W
Sbjct: 588 GMRWESRIVDHEDGPMQREKILVSVKSYW 616

  Database: nr
    Posted date:  Apr 1, 2003  2:05 AM
  Number of letters in database: 448,689,247
  Number of sequences in database:  1,393,205
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 476,102,324
Number of Sequences: 1393205
Number of extensions: 10489752
Number of successful extensions: 24480
Number of sequences better than 10.0: 86
Number of HSP's better than 10.0 without gapping: 23464
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24426
length of database: 448,689,247
effective HSP length: 116
effective length of database: 287,077,467
effective search space used: 18947112822
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

EST assemble image

clone accession position 1 MR038e11_f BP078955 1 390 2 MFB098f02_f BP041147 1 476 3 SPDL093b10_f BP057821 3 474 4 MWM235e12_f AV768319 6 552 5 MR031c08_f BP078371 13 388

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